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Questions and Answers
What type of media is used for the isolation of bacteria as pure culture?
What type of media is used for the isolation of bacteria as pure culture?
- Solid media
- Synthetic media
- Liquid media (correct)
- Routine laboratory media
Which characteristic makes agar an ideal solidifying agent in media?
Which characteristic makes agar an ideal solidifying agent in media?
- Agar liquefies at 37°C.
- Agar is bacteriologically inert. (correct)
- Agar is a protein extract.
- Agar is opaque.
Which type of media is used for profuse bacterial growth?
Which type of media is used for profuse bacterial growth?
- Liquid media (correct)
- Solid media
- Synthetic media
- Indicator media
Which type of bacteria grows in enriched media?
Which type of bacteria grows in enriched media?
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What additive is used to inhibit unwanted bacterial growth in selective media?
What additive is used to inhibit unwanted bacterial growth in selective media?
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Which is NOT a correct example of basal media?
Which is NOT a correct example of basal media?
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Which of these statements is true about Routine Laboratory Media?
Which of these statements is true about Routine Laboratory Media?
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Which component in Indicator media undergoes a visible change due to bacterial activity?
Which component in Indicator media undergoes a visible change due to bacterial activity?
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Study Notes
Media Classification by Consistency
- Solid media:
- Advantages: identification of bacteria by colony character, separation of mixed bacteria, and isolation of bacteria as pure culture
- Agar is commonly used to prepare solid media, which is:
- Bacteriologically inert
- Remains solid at 37°C
- Transparent
- Liquid media:
- Used for profuse growth, e.g., blood culture
- Mixed organisms cannot be separated
Media Classification by Chemical Composition
- Routine Laboratory Media:
- Chemically defined media prepared from pure chemical substances
- Used in research work
- Synthetic Media:
- Classified into six types:
- Basal media
- Enriched media
- Selective media
- Indicator media
- Transport media
- Storage media
- Classified into six types:
Synthetic Media Types
Basal Media
- Used for growth of bacteria that do not need enrichment
- Examples: Nutrient broth, nutrient agar, and peptone water
- Suitable for growth of Staphylococcus and Enterobacteriaceae
Enriched Media
- Enriched by adding blood, serum, or egg
- Examples: Blood agar and Lowenstein-Jensen media
- Suitable for growth of Streptococci
Selective Media
- Favour growth of a particular bacterium by inhibiting undesired bacteria
- Examples: MacConkey agar, Lowenstein-Jensen media, and tellurite media
- Antibiotic may be added to inhibit growth of undesired bacteria
Indicator Media
- Contain an indicator that changes in response to a particular organism
- Examples: Blood agar and MacConkey agar
- Indicator media are also known as differential media
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