Microbiology: Types of Media
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Questions and Answers

What type of media is used for the isolation of bacteria as pure culture?

  • Solid media
  • Synthetic media
  • Liquid media (correct)
  • Routine laboratory media
  • Which characteristic makes agar an ideal solidifying agent in media?

  • Agar liquefies at 37°C.
  • Agar is bacteriologically inert. (correct)
  • Agar is a protein extract.
  • Agar is opaque.
  • Which type of media is used for profuse bacterial growth?

  • Liquid media (correct)
  • Solid media
  • Synthetic media
  • Indicator media
  • Which type of bacteria grows in enriched media?

    <p>Streptococci</p> Signup and view all the answers

    What additive is used to inhibit unwanted bacterial growth in selective media?

    <p>Antibiotic</p> Signup and view all the answers

    Which is NOT a correct example of basal media?

    <p>Lowenstein-Jensen media</p> Signup and view all the answers

    Which of these statements is true about Routine Laboratory Media?

    <p>These are chemically defined media used in research work.</p> Signup and view all the answers

    Which component in Indicator media undergoes a visible change due to bacterial activity?

    <p>Neutral red</p> Signup and view all the answers

    Study Notes

    Media Classification by Consistency

    • Solid media:
      • Advantages: identification of bacteria by colony character, separation of mixed bacteria, and isolation of bacteria as pure culture
      • Agar is commonly used to prepare solid media, which is:
        • Bacteriologically inert
        • Remains solid at 37°C
        • Transparent
    • Liquid media:
      • Used for profuse growth, e.g., blood culture
      • Mixed organisms cannot be separated

    Media Classification by Chemical Composition

    • Routine Laboratory Media:
      • Chemically defined media prepared from pure chemical substances
      • Used in research work
    • Synthetic Media:
      • Classified into six types:
        • Basal media
        • Enriched media
        • Selective media
        • Indicator media
        • Transport media
        • Storage media

    Synthetic Media Types

    Basal Media

    • Used for growth of bacteria that do not need enrichment
    • Examples: Nutrient broth, nutrient agar, and peptone water
    • Suitable for growth of Staphylococcus and Enterobacteriaceae

    Enriched Media

    • Enriched by adding blood, serum, or egg
    • Examples: Blood agar and Lowenstein-Jensen media
    • Suitable for growth of Streptococci

    Selective Media

    • Favour growth of a particular bacterium by inhibiting undesired bacteria
    • Examples: MacConkey agar, Lowenstein-Jensen media, and tellurite media
    • Antibiotic may be added to inhibit growth of undesired bacteria

    Indicator Media

    • Contain an indicator that changes in response to a particular organism
    • Examples: Blood agar and MacConkey agar
    • Indicator media are also known as differential media

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    Description

    This quiz covers the different types of media in microbiology, including their consistency and chemical composition, with a focus on solid media and its advantages.

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