Microbiology Quiz on Bacterial Media and Stains
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Questions and Answers

Which medium is used for the cultivation of Corynebacterium diphtheriae?

  • Lowenstein-Jensen
  • Bordet-Gengou
  • Chocolate Agar Plate
  • Loeffler’s medium (correct)
  • What is the primary function of ribosomes in bacterial cells?

  • Cell wall formation
  • Nucleic acid replication
  • Protein synthesis (correct)
  • Energy production
  • Which bacterium is specifically associated with the medium called Skirrow?

  • Mycobacterium
  • Bordetella pertussis
  • Leptospira
  • Helicobacter pylori (correct)
  • What type of stain is Giemsa classified as?

    <p>Differential stain (B)</p> Signup and view all the answers

    Which organism is commonly identified using the Lowenstein-Jensen medium?

    <p>Mycobacterium (A)</p> Signup and view all the answers

    What is the primary function of enrichment media in bacterial culture?

    <p>To selectively promote the growth of fastidious organisms (B)</p> Signup and view all the answers

    Which of the following is an example of differential media?

    <p>Mac Conkey agar (B)</p> Signup and view all the answers

    What is a unique feature of enriched media compared to simple media?

    <p>Includes added supplements like blood or nutrients (A)</p> Signup and view all the answers

    Which process involves a donor cell releasing DNA that is then absorbed by a recipient cell?

    <p>Transformation (B)</p> Signup and view all the answers

    Which of the following is NOT a macronutrient essential for bacterial growth?

    <p>Iron (A)</p> Signup and view all the answers

    What type of media would you use if you want to grow bacteria that require specific nutrients to thrive?

    <p>Enrichment media (B)</p> Signup and view all the answers

    What is the main purpose of using nutrient agar in the laboratory?

    <p>To provide a basic growth medium for bacteria (A)</p> Signup and view all the answers

    Which of the following is a form of energy production utilized by bacteria?

    <p>Respiration (B)</p> Signup and view all the answers

    What is the most likely causative agent for the patient's condition considering the provided information?

    <p>Enterococcus spp. (B)</p> Signup and view all the answers

    Which bacterial genus is noted for its gamma hemolysis among the options listed?

    <p>Enterococcus (C)</p> Signup and view all the answers

    In the context of the patient's surgery, which type of endocarditis is most likely associated with Enterococcus?

    <p>Subacute endocarditis (B)</p> Signup and view all the answers

    In cases of infective endocarditis, which bacterium is commonly associated with prosthetic valve infections?

    <p>Staphylococcus epidermidis (C)</p> Signup and view all the answers

    What is a common characteristic of Streptococcus bovis in relation to endocarditis?

    <p>Is usually linked to gastrointestinal pathology (B)</p> Signup and view all the answers

    Which condition is indicated by the patient's symptoms of chest pain and tachycardia two days post-operation?

    <p>Infective endocarditis (C)</p> Signup and view all the answers

    What indicates the type of endocarditis if there is no underlying heart condition as seen in the patient?

    <p>Acute endocarditis (D)</p> Signup and view all the answers

    Which of the following statements about Clostridium species is true?

    <p>They are associated with gas gangrene and other soft tissue infections. (B)</p> Signup and view all the answers

    Which of the following streptococci are known to be resistant to bacitracin?

    <p>Streptococcus agalactiae (B)</p> Signup and view all the answers

    What is the primary virulence factor associated with Group A Streptococcus, Streptococcus pyogenes?

    <p>M protein (A)</p> Signup and view all the answers

    Which organism is classified as gamma hemolytic and grows in hypertonic solutions?

    <p>Enterococcus faecium (A)</p> Signup and view all the answers

    What is the likely diagnosis for a four-year-old boy presenting with a scarlatiniform rash, strawberry tongue, and high-grade fever?

    <p>Scarlet Fever (A)</p> Signup and view all the answers

    Which type of hemolysis is characterized by complete lysis of red blood cells?

    <p>Beta hemolytic (D)</p> Signup and view all the answers

    What important sequela should be monitored in patients with Group A Streptococcus infections?

    <p>Rheumatic heart disease (D)</p> Signup and view all the answers

    What bacteria is classified as Group D and is known for its growth characteristics?

    <p>Streptococcus bovis (D)</p> Signup and view all the answers

    Which streptococci produce hydrogen peroxide and exhibit resistance?

    <p>Streptococcus salivarius (A)</p> Signup and view all the answers

    What is the primary effect of the heat-stable enterotoxin produced by Staphylococcus?

    <p>Functions as a superantigen (B)</p> Signup and view all the answers

    How long is the mean incubation period for emetic food poisoning caused by rice?

    <p>9 hours (C)</p> Signup and view all the answers

    What type of toxin does Clostridium botulinum produce?

    <p>Botulinum toxin (B)</p> Signup and view all the answers

    What symptom is specifically associated with Clostridium difficile infections?

    <p>Severe diarrhea (A)</p> Signup and view all the answers

    What laboratory test is used to identify the botulinum toxin?

    <p>Mouse Neutralization Test (A)</p> Signup and view all the answers

    Which food type is commonly linked to Clostridium perfringens poisoning?

    <p>Meat and vegetables (A)</p> Signup and view all the answers

    What is the mean duration of diarrhea caused by heat-labile enterotoxin?

    <p>20-36 hours (C)</p> Signup and view all the answers

    What serious condition is associated with the alpha-toxin of Clostridium perfringens?

    <p>Gas gangrene (A)</p> Signup and view all the answers

    Which of the following proteins prevents the fusion of phagolysosomes in neutrophils?

    <p>Opa proteins (C)</p> Signup and view all the answers

    What is the primary mode of action of beta-lactamase in gonococci?

    <p>Degrades beta-lactam rings in penicillin (B)</p> Signup and view all the answers

    Which specimen type is associated with a presumptive diagnosis of gonorrhea?

    <p>Endocervix (B)</p> Signup and view all the answers

    What virulence factor is primarily responsible for the endotoxic activity of gonococci?

    <p>Lipo-oligosaccharides (B)</p> Signup and view all the answers

    Which serotypes are primarily associated with the most cases of Neisseria meningitidis infection?

    <p>A, B, C (D)</p> Signup and view all the answers

    What is the most common anatomical site for the carriage of Neisseria meningitidis in humans?

    <p>Nares (C)</p> Signup and view all the answers

    Which treatment is effective against Neisseria meningitidis?

    <p>Chloramphenicol (D)</p> Signup and view all the answers

    Which statement regarding the disease process of Neisseria meningitidis is true?

    <p>Endotoxin causes hemorrhage and shock. (B)</p> Signup and view all the answers

    Which protein produced by gonococci helps in blocking serum bactericidal activity?

    <p>Rmp proteins (C)</p> Signup and view all the answers

    Which of the following statements correctly describes a characteristic of Neisseria species?

    <p>They can cause asymptomatic carriage. (A)</p> Signup and view all the answers

    Flashcards

    TCBS Agar

    A type of agar plate specifically designed for the cultivation of Vibrio cholerae.

    Loeffler's Medium

    A type of medium used to grow Corynebacterium diphtheriae, a bacterium responsible for diphtheria.

    Skirrow's Medium

    A type of medium used to grow Helicobacter pylori, a bacterium that commonly causes gastritis and ulcers.

    Lowenstein-Jensen Medium

    A type of medium used to grow Mycobacterium, a genus of bacteria that includes the one responsible for tuberculosis.

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    Bordet-Gengou Medium

    A type of medium used to grow Bordetella pertussis, a bacteria that causes whooping cough.

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    Simple/General Media

    A type of medium used in the lab that contains specific nutrients to support the growth of various bacteria. This medium is used in the laboratory without the addition of any extra supplements.

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    Enriched Media

    A type of medium designed to enhance the growth of fastidious organisms that require additional nutrients for survival. These mediums contain specific supplements added to the basic media.

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    Differential Media

    This type of media allows visualization of metabolic differences between groups of bacteria based on how they grow and interact with the medium.

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    Transformation

    The process by which a bacterium acquires a new genetic trait through the uptake of naked DNA from its environment. The donor cell lyses, releasing a fragment of its DNA which can then be taken up by a recipient cell.

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    Group D Streptococci

    A group of bacteria that are part of the normal flora of the intestines and oral cavity. Majority produce gamma hemolysis.

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    Acute Endocarditis

    A type of endocarditis that presents suddenly and aggressively, often in individuals without underlying heart problems. Involves native valves, commonly caused by Staphylococcus aureus.

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    Subacute Endocarditis

    A type of endocarditis that develops gradually, often in individuals with underlying heart conditions. Involves native or prosthetic valves, and is commonly associated with Viridans streptococci.

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    Endocarditis after GIT surgery

    A type of endocarditis that occurs after gastrointestinal surgery, often caused by Enterococcus.

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    Marantic Endocarditis

    A type of endocarditis that occurs in the presence of underlying conditions such as cancer or malnutrition. Often caused by Streptococcus bovis.

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    Staphylococcus aureus

    A type of bacteria commonly associated with acute endocarditis, particularly in native valves.

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    Staphylococcus epidermidis

    A type of bacteria commonly associated with endocarditis, particularly in prosthetic valves.

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    Streptococcus bovis

    A type of bacteria that can cause endocarditis and is commonly associated with Marantic endocarditis.

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    Viridans Streptococci (H2O2 resistant)

    Streptococci that tolerate the presence of hydrogen peroxide, including S. angionosus, S. salivarius, S. mutans, S. sanguis, and S. mitis.

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    Beta Hemolysis

    A type of hemolysis where red blood cells are completely lysed around bacterial colonies, resulting in a clear zone on a blood agar plate.

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    Gamma Hemolysis

    Streptococci that lack the ability to produce hemolysins, resulting in no lysis of red blood cells on a blood agar plate.

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    Lancefield Classification

    The classification of streptococci based on their antigenic characteristics, using serologic methods to differentiate them.

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    Group A Streptococcus (S. pyogenes)

    The most important serotype of Streptococcus pyogenes, known for its ability to cause scarlet fever and other serious infections.

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    Group B Streptococcus (S. agalactiae)

    A type of Streptococcus commonly found in the vagina and rectum, particularly associated with neonatal infections.

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    Enterococci (Group D)

    A group of bacteria that withstand high salt concentrations, commonly found in the gastrointestinal tract.

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    Erythrogenic Toxin

    A virulence factor of Streptococcus pyogenes, responsible for the characteristic scarlet fever rash.

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    What is the function of Por proteins in Neisseria gonorrhoeae?

    These proteins help Neisseria gonorrhoeae evade the immune system by preventing the formation of phagolysosomes within neutrophils, thus preventing the bacteria from being killed.

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    What role do Opa proteins play in Neisseria gonorrhoeae?

    Opa proteins are responsible for Neisseria gonorrhoeae adherence to both other bacterial cells and eukaryotic cells.

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    How do Rmp proteins contribute to Neisseria gonorrhoeae's survival?

    Rmp proteins help Neisseria gonorrhoeae evade the immune system by inducing the production of antibodies that block serum bactericidal activity against the bacteria.

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    What are LOS and what is their significance in Neisseria gonorrhoeae?

    Lipooligosaccharides (LOS) are found on the surface of Neisseria gonorrhoeae. They have endotoxic activity, meaning they can cause fever and inflammation.

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    What is IgA protease and what does it do?

    IgA protease is an enzyme produced by Neisseria gonorrhoeae that breaks down IgA antibodies, which are important for mucosal immunity.

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    What is beta-lactamase and what is its role in Neisseria gonorrhoeae?

    Beta-lactamase is an enzyme produced by Neisseria gonorrhoeae that breaks down penicillin and similar antibiotics.

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    What are plasmids in Neisseria gonorrhoeae and what is their significance?

    Plasmids in Neisseria gonorrhoeae often carry genes that confer resistance to antibiotics, making treatment more challenging.

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    What is Neisseria meningitidis and why is it important to understand?

    Meningococcal disease is usually caused by Neisseria meningitidis. This bacteria can cause serious infections like meningitis and septicemia.

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    What is the most common form of Neisseria meningitidis infection?

    The most common form of Neisseria meningitidis infection is asymptomatic carriage, meaning the bacteria are present in the body without causing symptoms.

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    How does Neisseria meningitidis infection progress?

    Meningococcal disease develops when the bacteria enter the bloodstream, reach the meninges, and cause inflammation. The onset is rapid and can be fatal.

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    Emetic Bacillus cereus food poisoning

    A type of food poisoning caused by Bacillus cereus, characterized by vomiting and abdominal cramps, with a shorter duration (8-10 hours). It is linked to heat-stable enterotoxin, similar to staphylococcal food poisoning.

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    Diarrheal Bacillus cereus food poisoning

    A type of food poisoning caused by Bacillus cereus, characterized by diarrhea, nausea, and abdominal cramps, lasting longer (20-36 hours). It is associated with heat-labile enterotoxin, resembling clostridial gastroenteritis.

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    Botulinum toxin

    A potent neurotoxin produced by Clostridium botulinum, which causes paralysis by blocking the release of acetylcholine at the synapse.

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    Botulism

    A type of food poisoning caused by Clostridium botulinum, leading to flaccid paralysis due to the blockage of acetylcholine release by the botulinum toxin. It can be fatal.

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    Clostridium perfringens food poisoning

    A common cause of food poisoning, characterized by diarrhea, nausea, and abdominal cramps. It is caused by Clostridium perfringens, which produces an alpha-toxin (lecithinase) detectable by Nagler's reaction.

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    Clostridium difficile infection

    A common cause of superinfection, usually occurring after antibiotic treatment, leading to severe diarrhea, abdominal cramping, and fever. It is caused by Clostridium difficile.

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    Pseudomembranous colitis

    A condition characterized by the formation of a pseudomembrane in the colon, usually caused by Clostridium difficile. It presents with severe diarrhea, abdominal cramps, and fever.

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    Superantigen

    A type of toxin that binds to major histocompatibility complex (MHC) molecules on antigen-presenting cells, causing an overactive immune response, leading to inflammation and tissue damage.

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    Study Notes

    Table of Contents

    • Introduction and Bacterial Recombination (Dr. Dacanay)
    • Introduction to Bacteriology and Gram-Positive Bacteria (Dr. Dacanay)
    • Streptococci (Dr. Dacanay)
    • Gram-Negative Bacteria (Dr. Dacanay)
    • Spore-forming and Non-Spore-Forming Rods (Dr. Dacanay)
    • Enterics (Dr. Dacanay)
    • Zoonotic Infections, Spirochetes and Atypical Organisms (Dr. Dacanay)
    • Medical Mycology and Pharmacology of Antifungals (Dr. Dacanay)
    • Medical Virology (Dr. Cruz)
    • Medical Parasitology (Dr. Cruz)
    • Introduction to Parasitology
    • Protozoan Infections
    • Nematode Infections
    • Cestode Infections
    • Arthropods of Medical Importance
    • Medical Parasitology (Dr. Pata)

    RA 8293

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    Description

    Test your knowledge on the various media types used for bacterial cultivation and the specific functions of ribosomes in bacterial cells. This quiz covers important concepts such as enrichment media, differential media, and unique staining techniques in microbiology.

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