Podcast
Questions and Answers
What type of organisms are typically grown on Simple media?
What type of organisms are typically grown on Simple media?
- Fastidious organisms
- Fungi
- Non fastidious organisms (correct)
- Viruses
Which component is difficult to estimate in Complex media like Blood Agar (BA)?
Which component is difficult to estimate in Complex media like Blood Agar (BA)?
- Blood cells
- Peptones
- Carbohydrates
- Growth factors (correct)
What is the main purpose of Enriched media in bacterial culture?
What is the main purpose of Enriched media in bacterial culture?
- Support the growth of fastidious bacteria (correct)
- Provide a simple environment for bacterial growth
- Inhibit bacterial growth
- Detect yeasts and fungi
Which type of media is specifically used for research purposes?
Which type of media is specifically used for research purposes?
What does the addition of blood, serum, or extracts do to agar or broth in Enriched media?
What does the addition of blood, serum, or extracts do to agar or broth in Enriched media?
What is a distinguishing characteristic of Synthetic media like Davis & Mingioli?
What is a distinguishing characteristic of Synthetic media like Davis & Mingioli?
What is the purpose of selective media in bacterial culture?
What is the purpose of selective media in bacterial culture?
Which of the following substances is commonly used in selective media to inhibit the growth of coliforms?
Which of the following substances is commonly used in selective media to inhibit the growth of coliforms?
In differential media, how is E. coli differentiated from Salmonella typhi on Mac Conkey agar?
In differential media, how is E. coli differentiated from Salmonella typhi on Mac Conkey agar?
What are the macronutrients necessary for bacterial growth?
What are the macronutrients necessary for bacterial growth?
Which type of organisms can grow on Mannitol Salt Agar (MSA) due to its high salt concentration?
Which type of organisms can grow on Mannitol Salt Agar (MSA) due to its high salt concentration?
Which type of media is suitable to grow bacteria when the numbers in the inoculum are suspected to be low?
Which type of media is suitable to grow bacteria when the numbers in the inoculum are suspected to be low?
What is the distinguishing characteristic between haemolytic and non-haemolytic organisms on blood agar?
What is the distinguishing characteristic between haemolytic and non-haemolytic organisms on blood agar?
What is the main characteristic of solid media for bacterial culture?
What is the main characteristic of solid media for bacterial culture?
What is the function of biphasic media in bacterial culture?
What is the function of biphasic media in bacterial culture?
What metabolic process occurs if mannitol is fermented on Mannitol Salt Agar (MSA)?
What metabolic process occurs if mannitol is fermented on Mannitol Salt Agar (MSA)?
Which nutrients may some bacteria need to be fed in addition to a carbon source for growth?
Which nutrients may some bacteria need to be fed in addition to a carbon source for growth?
What is the primary purpose of selective media in bacterial culture?
What is the primary purpose of selective media in bacterial culture?
Flashcards are hidden until you start studying
Study Notes
Simple Media
- Typically grows fast-growing, non-fastidious organisms
Complex Media
- Blood Agar (BA) has a difficult-to-estimate level of nutrients in the red blood cells.
Enriched Media
- Enriched media provides additional nutrients to support the growth of fastidious organisms
Research Media
- Synthetic media like Davis & Mingioli is specifically designed for research purposes due to its precisely defined chemical composition
Enriched Media Additions
- Adding blood, serum, or extracts to agar or broth provides additional growth factors and nutrients for fastidious organisms
Synthetic Media
- Synthetic media like Davis & Mingioli has a precisely defined chemical composition, which is essential for research.
Selective Media
- Selective media promotes the growth of certain microorganisms while inhibiting the growth of others.
Selective Media Substance
- Bile salts are commonly used in selective media to inhibit the growth of coliforms.
Differential Media
- E.coli ferments lactose, producing acid and turning the medium pink on Mac Conkey agar, while Salmonella typhi does not ferment lactose, leaving the medium unchanged.
Macronutrients
- Carbon, nitrogen, phosphorus, sulfur, potassium, magnesium, calcium, and iron are the essential macronutrients for bacterial growth.
Mannitol Salt Agar (MSA)
- MSA's high salt concentration allows halophilic organisms (salt-loving bacteria) to grow
Low Inoculum Growth
- Enriched media is suitable for growing bacteria when the numbers in the inoculum are suspected to be low
Haemolytic vs Non-Haemolytic Organisms
- Haemolytic organisms on blood agar cause hemolysis (breakdown of red blood cells), resulting in a clearing around the colony, whereas non-haemolytic organisms do not.
Solid Media
- Solid media provides a surface for bacterial growth and allows for colony isolation and characterization.
Biphasic Media
- Biphasic media, with both solid and liquid phases, is used for culturing bacteria that require an interface for growth
Mannitol Fermentation
- Mannitol fermentation on MSA causes acid production, resulting in a yellow halo around the colony.
Additional Nutrient Needs
- Some bacteria require specific growth factors like vitamins, amino acids, or purines in addition to a carbon source.
Selective Media Purpose
- Selective media inhibits the growth of unwanted microorganisms and allows the growth of specific target organisms.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
