18 Questions
What type of organisms are typically grown on Simple media?
Non fastidious organisms
Which component is difficult to estimate in Complex media like Blood Agar (BA)?
Growth factors
What is the main purpose of Enriched media in bacterial culture?
Support the growth of fastidious bacteria
Which type of media is specifically used for research purposes?
Synthetic media
What does the addition of blood, serum, or extracts do to agar or broth in Enriched media?
Support the growth of fastidious bacteria
What is a distinguishing characteristic of Synthetic media like Davis & Mingioli?
Every component is well known
What is the purpose of selective media in bacterial culture?
Inhibit the growth of specific bacterial groups while allowing the growth of others
Which of the following substances is commonly used in selective media to inhibit the growth of coliforms?
Bile salts
In differential media, how is E. coli differentiated from Salmonella typhi on Mac Conkey agar?
E. coli turns red-pink
What are the macronutrients necessary for bacterial growth?
C, H, O, N, P, S, K+, Ca2+
Which type of organisms can grow on Mannitol Salt Agar (MSA) due to its high salt concentration?
Staphylococcus spp.
Which type of media is suitable to grow bacteria when the numbers in the inoculum are suspected to be low?
Liquid (broth)
What is the distinguishing characteristic between haemolytic and non-haemolytic organisms on blood agar?
Partial lysis for haemolytic organisms
What is the main characteristic of solid media for bacterial culture?
Contains agar and solidifies
What is the function of biphasic media in bacterial culture?
Combines the benefits of both liquid and solid media
What metabolic process occurs if mannitol is fermented on Mannitol Salt Agar (MSA)?
Acidic products are formed
Which nutrients may some bacteria need to be fed in addition to a carbon source for growth?
Vitamins, amino acids, blood
What is the primary purpose of selective media in bacterial culture?
Promote the growth of specific bacteria while inhibiting others
Test your knowledge on bacterial growth media with this quiz. Learn about the indicators for differentiating pathogenic and non-pathogenic Staphylococcus, as well as the use of enriched media for supporting the growth of fastidious bacteria.
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