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Questions and Answers
What is the primary advantage of autoclaving in the sterilization process?
What is the primary advantage of autoclaving in the sterilization process?
Which temperature is typically achieved in an autoclave for effective sterilization?
Which temperature is typically achieved in an autoclave for effective sterilization?
What is the process known as fractional sterilization primarily aimed at?
What is the process known as fractional sterilization primarily aimed at?
Which of the following is a correct description of pasteurization?
Which of the following is a correct description of pasteurization?
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In fractional sterilization, what happens after the initial exposure to steam?
In fractional sterilization, what happens after the initial exposure to steam?
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Why is steam pressure critical in the autoclaving process?
Why is steam pressure critical in the autoclaving process?
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Which microorganism is targeted specifically during pasteurization?
Which microorganism is targeted specifically during pasteurization?
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Study Notes
Autoclaving
- Uses steam pressure to increase sterilization temperature and reduce time.
- Maintains pressure 15 lb/sq inch above atmospheric pressure.
- Superheats water molecules to at least 121.5°C.
- Rapidly transfers heat to items (up to 15 meters).
- Commonly used to sterilize culture media, glassware, and metalware.
Fractional Sterilization (Tyndallization)
- Performed in a cyclical, on-off sterilization process.
- Initial sterilization with free-flowing steam at 100°C for 30 minutes.
- Allowed to cool to let bacterial spores germinate into vegetative cells.
- Resterilized one to three times depending on bacterial growth.
Pasteurization
- Reduces bacterial population of liquids (like milk).
- Heats liquids to 62.9°C for 30 minutes.
- Kills Mycobacterium tuberculosis and Q-fever agents.
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Description
This quiz covers key sterilization techniques in microbiology, including autoclaving, fractional sterilization, and pasteurization. Understand the processes, temperatures, and applications of each method to ensure effective sterilization in laboratory settings.
