Microbiology: Staining Procedures
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Questions and Answers

What is the primary reason for staining microorganisms in microscopy?

  • To resolve the problem of microorganisms appearing colorless (correct)
  • To observe the shape and size of the microorganisms
  • To identify the type of microorganisms
  • To determine the pH of the microorganisms

    • What is the composition of stains?

  • A complex salt of a dye acid with a dye base
  • A mixture of a cation and an anion (correct)
  • A mixture of a basic dye and an acidic dye
  • A mixture of a chromophore and a chromogen

    • What is the role of the chromophore in a stain?

  • It is the negatively charged ion in the stain
  • It is the complex salt of a dye acid with a dye base
  • It is the positively charged ion in the stain
  • It is the unsaturated group of molecules capable of producing color (correct)

    • Why are basic dyes more frequently used for staining bacteria?

    <p>Because the colored cation is attracted to the negatively charged bacterial cell</p> Signup and view all the answers

    What is the result of using acidic dyes for staining bacteria?

    <p>The background is stained, but the bacteria are not</p> Signup and view all the answers

    What is the purpose of negative staining?

    <p>To observe the shape and size of the bacteria</p> Signup and view all the answers

    What is a neutral stain composed of?

    <p>A complex salt of a dye acid with a dye base</p> Signup and view all the answers

    What is the term for a compound that contains a chromophore?

    <p>Chromogen</p> Signup and view all the answers

    What is the net charge of bacteria at a pH of 7?

    <p>Slightly negative</p> Signup and view all the answers

    What can be seen more easily using negative staining?

    <p>Overall cell shape, size and capsules</p> Signup and view all the answers

    What type of ion is the chromophore in an acidic dye?

    <p>Anion</p> Signup and view all the answers

    Why do basic dyes bind to bacterial cells?

    <p>Because the bacterial cell is negatively charged</p> Signup and view all the answers

    What is the name of the complex salt of a dye acid with a dye base?

    <p>Neutral stain</p> Signup and view all the answers

    What is the term for an unsaturated group of molecules that produces color?

    <p>Chromophore</p> Signup and view all the answers

    What is the purpose of staining microorganisms?

    <p>To make the microorganisms visible under a microscope</p> Signup and view all the answers

    What is the result of using a basic dye on a bacterial cell?

    <p>The bacterial cell is stained</p> Signup and view all the answers

    What type of charge does a bacterial cell have at pH 7?

    <p>Negatively charged</p> Signup and view all the answers

    What is the term for a compound that contains a chromophore?

    <p>Chromogen</p> Signup and view all the answers

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