Microbiology: Staining Procedures
18 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary reason for staining microorganisms in microscopy?

  • To resolve the problem of microorganisms appearing colorless (correct)
  • To observe the shape and size of the microorganisms
  • To identify the type of microorganisms
  • To determine the pH of the microorganisms

What is the composition of stains?

  • A complex salt of a dye acid with a dye base
  • A mixture of a cation and an anion (correct)
  • A mixture of a basic dye and an acidic dye
  • A mixture of a chromophore and a chromogen

What is the role of the chromophore in a stain?

  • It is the negatively charged ion in the stain
  • It is the complex salt of a dye acid with a dye base
  • It is the positively charged ion in the stain
  • It is the unsaturated group of molecules capable of producing color (correct)

Why are basic dyes more frequently used for staining bacteria?

<p>Because the colored cation is attracted to the negatively charged bacterial cell (C)</p> Signup and view all the answers

What is the result of using acidic dyes for staining bacteria?

<p>The background is stained, but the bacteria are not (A)</p> Signup and view all the answers

What is the purpose of negative staining?

<p>To observe the shape and size of the bacteria (A)</p> Signup and view all the answers

What is a neutral stain composed of?

<p>A complex salt of a dye acid with a dye base (B)</p> Signup and view all the answers

What is the term for a compound that contains a chromophore?

<p>Chromogen (C)</p> Signup and view all the answers

What is the net charge of bacteria at a pH of 7?

<p>Slightly negative (A)</p> Signup and view all the answers

What can be seen more easily using negative staining?

<p>Overall cell shape, size and capsules (B)</p> Signup and view all the answers

What type of ion is the chromophore in an acidic dye?

<p>Anion (B)</p> Signup and view all the answers

Why do basic dyes bind to bacterial cells?

<p>Because the bacterial cell is negatively charged (C)</p> Signup and view all the answers

What is the name of the complex salt of a dye acid with a dye base?

<p>Neutral stain (D)</p> Signup and view all the answers

What is the term for an unsaturated group of molecules that produces color?

<p>Chromophore (D)</p> Signup and view all the answers

What is the purpose of staining microorganisms?

<p>To make the microorganisms visible under a microscope (B)</p> Signup and view all the answers

What is the result of using a basic dye on a bacterial cell?

<p>The bacterial cell is stained (B)</p> Signup and view all the answers

What type of charge does a bacterial cell have at pH 7?

<p>Negatively charged (D)</p> Signup and view all the answers

What is the term for a compound that contains a chromophore?

<p>Chromogen (D)</p> Signup and view all the answers

More Like This

Use Quizgecko on...
Browser
Browser