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Microbiology: Staining and Microscopy

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Questions and Answers

What is the primary function of staining in microbiology?

To enhance the contrast of microbes for visualization (correct)

To kill microbes

To provide nutrition to microbes

To separate microbes from each other

What is the purpose of the decolorizer in the Gram staining process?

To add a counterstain to gram negative cells

To add color to gram positive cells

To wash away the stain from gram negative cell walls (correct)

To remove excess stain from gram positive cell walls

What is the difference between gram positive and gram negative bacteria with regards to their cell walls?

Gram positive has a cell wall, gram negative does not

Gram positive has a thick peptidoglycan layer, gram negative has a thin layer (correct)

Gram positive has an outer membrane, gram negative does not

Gram positive has a thin peptidoglycan layer, gram negative has a thick layer

What is the name of the primary stain used in the Gram staining process?

Crystal Violet Signup and view all the answers

What is the function of the counterstain in the Gram staining process?

To stain gram negative cells pink Signup and view all the answers

What is the purpose of the iodine mordant in the Gram staining process?

To make the primary stain less soluble so it adheres to cell walls Signup and view all the answers

What is the main function of peptidoglycan in the bacterial cell wall?

To provide tensile strength in two dimensions Signup and view all the answers

What is the main difference between Gram Positive and Gram Negative bacteria?

The thickness of the peptidoglycan layer Signup and view all the answers

What is the function of teichoic acid in the Gram Positive bacterial cell wall?

To enhance the rigidity of the cell wall Signup and view all the answers

What is the main characteristic of Archaea?

They live in extreme environments Signup and view all the answers

What is the purpose of taxonomy in microbiology?

To identify and describe microorganisms Signup and view all the answers

What is the significance of the discovery of Mycoplasma?

They lack a cell wall Signup and view all the answers