12 Questions
What is the primary function of staining in microbiology?
To enhance the contrast of microbes for visualization
What is the purpose of the decolorizer in the Gram staining process?
To wash away the stain from gram negative cell walls
What is the difference between gram positive and gram negative bacteria with regards to their cell walls?
Gram positive has a thick peptidoglycan layer, gram negative has a thin layer
What is the name of the primary stain used in the Gram staining process?
Crystal Violet
What is the function of the counterstain in the Gram staining process?
To stain gram negative cells pink
What is the purpose of the iodine mordant in the Gram staining process?
To make the primary stain less soluble so it adheres to cell walls
What is the main function of peptidoglycan in the bacterial cell wall?
To provide tensile strength in two dimensions
What is the main difference between Gram Positive and Gram Negative bacteria?
The thickness of the peptidoglycan layer
What is the function of teichoic acid in the Gram Positive bacterial cell wall?
To enhance the rigidity of the cell wall
What is the main characteristic of Archaea?
They live in extreme environments
What is the purpose of taxonomy in microbiology?
To identify and describe microorganisms
What is the significance of the discovery of Mycoplasma?
They lack a cell wall
Learn about the different staining techniques used in microbiology to visualize microorganisms, including simple staining, differential staining, and special stains. Understand the principles of microscopy and the limitations of light microscopy. Identify the different types of stains, including acidic and basic dyes.
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