Microbiology: Staining and Microscopy
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Questions and Answers

What is the primary function of staining in microbiology?

  • To enhance the contrast of microbes for visualization (correct)
  • To kill microbes
  • To provide nutrition to microbes
  • To separate microbes from each other

What is the purpose of the decolorizer in the Gram staining process?

  • To add a counterstain to gram negative cells
  • To add color to gram positive cells
  • To wash away the stain from gram negative cell walls (correct)
  • To remove excess stain from gram positive cell walls

What is the difference between gram positive and gram negative bacteria with regards to their cell walls?

  • Gram positive has a cell wall, gram negative does not
  • Gram positive has a thick peptidoglycan layer, gram negative has a thin layer (correct)
  • Gram positive has an outer membrane, gram negative does not
  • Gram positive has a thin peptidoglycan layer, gram negative has a thick layer

What is the name of the primary stain used in the Gram staining process?

<p>Crystal Violet (C)</p> Signup and view all the answers

What is the function of the counterstain in the Gram staining process?

<p>To stain gram negative cells pink (A)</p> Signup and view all the answers

What is the purpose of the iodine mordant in the Gram staining process?

<p>To make the primary stain less soluble so it adheres to cell walls (C)</p> Signup and view all the answers

What is the main function of peptidoglycan in the bacterial cell wall?

<p>To provide tensile strength in two dimensions (D)</p> Signup and view all the answers

What is the main difference between Gram Positive and Gram Negative bacteria?

<p>The thickness of the peptidoglycan layer (C)</p> Signup and view all the answers

What is the function of teichoic acid in the Gram Positive bacterial cell wall?

<p>To enhance the rigidity of the cell wall (D)</p> Signup and view all the answers

What is the main characteristic of Archaea?

<p>They live in extreme environments (D)</p> Signup and view all the answers

What is the purpose of taxonomy in microbiology?

<p>To identify and describe microorganisms (D)</p> Signup and view all the answers

What is the significance of the discovery of Mycoplasma?

<p>They lack a cell wall (C)</p> Signup and view all the answers

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