16 Questions
What is the main factor influencing the level of detail we can observe in microscopic subjects?
Wavelength of radiation
In the electromagnetic spectrum, which type of radiation has the widest wavelength?
Radio waves
What type of radiation has the strongest spectrum in the electromagnetic spectrum?
Gamma rays
What happens to the image when light passes through a convex lens in a microscope?
The image is enlarged and inverted
What factor ensures that the microscopic specimen stands out through differences in intensity?
Contrast
Which factor provides the ability to distinguish two separate points as distinct in microscopy?
Resolution
Which type of microscope uses light in phase to enhance contrast?
Phase-contrast microscope
What does the dark-field microscope best used to see?
Translucent specimen
Which type of microscope uses UV light onto the specimen to increase resolution and contrast?
Fluorescence microscope
What does confocal microscopes use alongside fluorescent dyes to construct 3-D images?
Laser
What is the typical magnification range of electron microscopes?
100x to 100,000x
What is required for specimens when using an electron microscope?
Vacuum
Which type of contrast do phase-contrast microscopes enhance?
Contrast in light phases
What does dark-field microscopes direct onto the specimen to increase contrast?
Light from the sides at an angle
How is total magnification calculated in compound microscopes?
Magnification of ocular lens times magnification of objective lens
What does fluorescence microscopy use UV light onto the specimen for?
To increase resolution and contrast
Test your understanding of electromagnetic radiation in microscopy, the influence of wavelength on level of detail, magnification, resolution, contrast techniques such as staining, and the relevance of these concepts to microbiology.
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