BIOL 2260 Microscopy, Staining, and Classification Quiz

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Questions and Answers

What is the main factor influencing the level of detail we can observe in microscopic subjects?

  • Resolution
  • Contrast
  • Wavelength of radiation (correct)
  • Magnification

In the electromagnetic spectrum, which type of radiation has the widest wavelength?

  • Infrared
  • Microwave
  • Visible light
  • Radio waves (correct)

What type of radiation has the strongest spectrum in the electromagnetic spectrum?

  • Microwave
  • X-rays
  • UV light
  • Gamma rays (correct)

What happens to the image when light passes through a convex lens in a microscope?

<p>The image is enlarged and inverted (C)</p> Signup and view all the answers

What factor ensures that the microscopic specimen stands out through differences in intensity?

<p>Contrast (A)</p> Signup and view all the answers

Which factor provides the ability to distinguish two separate points as distinct in microscopy?

<p>Resolution (C)</p> Signup and view all the answers

Which type of microscope uses light in phase to enhance contrast?

<p>Phase-contrast microscope (D)</p> Signup and view all the answers

What does the dark-field microscope best used to see?

<p>Translucent specimen (A)</p> Signup and view all the answers

Which type of microscope uses UV light onto the specimen to increase resolution and contrast?

<p>Fluorescence microscope (D)</p> Signup and view all the answers

What does confocal microscopes use alongside fluorescent dyes to construct 3-D images?

<p>Laser (B)</p> Signup and view all the answers

What is the typical magnification range of electron microscopes?

<p>100x to 100,000x (A)</p> Signup and view all the answers

What is required for specimens when using an electron microscope?

<p>Vacuum (D)</p> Signup and view all the answers

Which type of contrast do phase-contrast microscopes enhance?

<p>Contrast in light phases (C)</p> Signup and view all the answers

What does dark-field microscopes direct onto the specimen to increase contrast?

<p>Light from the sides at an angle (B)</p> Signup and view all the answers

How is total magnification calculated in compound microscopes?

<p>Magnification of ocular lens times magnification of objective lens (C)</p> Signup and view all the answers

What does fluorescence microscopy use UV light onto the specimen for?

<p>To increase resolution and contrast (A)</p> Signup and view all the answers

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