BIOL 2260 Microscopy, Staining, and Classification Quiz
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Questions and Answers

What is the main factor influencing the level of detail we can observe in microscopic subjects?

  • Resolution
  • Contrast
  • Wavelength of radiation (correct)
  • Magnification
  • In the electromagnetic spectrum, which type of radiation has the widest wavelength?

  • Infrared
  • Microwave
  • Visible light
  • Radio waves (correct)
  • What type of radiation has the strongest spectrum in the electromagnetic spectrum?

  • Microwave
  • X-rays
  • UV light
  • Gamma rays (correct)
  • What happens to the image when light passes through a convex lens in a microscope?

    <p>The image is enlarged and inverted</p> Signup and view all the answers

    What factor ensures that the microscopic specimen stands out through differences in intensity?

    <p>Contrast</p> Signup and view all the answers

    Which factor provides the ability to distinguish two separate points as distinct in microscopy?

    <p>Resolution</p> Signup and view all the answers

    Which type of microscope uses light in phase to enhance contrast?

    <p>Phase-contrast microscope</p> Signup and view all the answers

    What does the dark-field microscope best used to see?

    <p>Translucent specimen</p> Signup and view all the answers

    Which type of microscope uses UV light onto the specimen to increase resolution and contrast?

    <p>Fluorescence microscope</p> Signup and view all the answers

    What does confocal microscopes use alongside fluorescent dyes to construct 3-D images?

    <p>Laser</p> Signup and view all the answers

    What is the typical magnification range of electron microscopes?

    <p>100x to 100,000x</p> Signup and view all the answers

    What is required for specimens when using an electron microscope?

    <p>Vacuum</p> Signup and view all the answers

    Which type of contrast do phase-contrast microscopes enhance?

    <p>Contrast in light phases</p> Signup and view all the answers

    What does dark-field microscopes direct onto the specimen to increase contrast?

    <p>Light from the sides at an angle</p> Signup and view all the answers

    How is total magnification calculated in compound microscopes?

    <p>Magnification of ocular lens times magnification of objective lens</p> Signup and view all the answers

    What does fluorescence microscopy use UV light onto the specimen for?

    <p>To increase resolution and contrast</p> Signup and view all the answers

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