Microbiology SIM Test Overview
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Questions and Answers

What does the sulfate indole motility (SIM) tube test for?

  • Sulfate reduction, indole production, and motility (correct)
  • Amino acid deamination, hydrogen sulfide, and fermentation
  • Fermentation, nitrate reduction, and motility
  • Protein synthesis, nitrate reduction, and indole production

The production of hydrogen sulfide (H2S) in a SIM test is an aerobic process.

False (B)

What reagent must be added to a culture tube to visualize indole production?

Kovac’s reagent

The _____ produced when sulfate salts are metabolized will form a BLACK insoluble metal sulfide precipitate.

<p>H2S</p> Signup and view all the answers

Which of the following procedures is NOT part of the sulfate indole motility test?

<p>Adding glucose as a carbon source (C)</p> Signup and view all the answers

Match the bacteria with their common characteristics:

<p>Staphylococcus aureus = Gram-positive cocci Escherichia coli = Gram-negative bacilli Bacillus subtilis = Endospore-forming Proteus vulgaris = Known for urease production Pseudomonas aeruginosa = Opportunistic pathogen Enterobacter aerogenes = Lactose fermenter</p> Signup and view all the answers

Indole is created from the breakdown of the amino acid lysine.

<p>False (B)</p> Signup and view all the answers

In the motility test using SIM media, how can you determine motility?

<p>By observing the diffusion of the inoculum from the stab line</p> Signup and view all the answers

What temperature should the bacteria be incubated at during the tests?

<p>37°C (B)</p> Signup and view all the answers

A yellow color in the phenylalanine deaminase test indicates a positive result.

<p>False (B)</p> Signup and view all the answers

What color change indicates a positive result for the indole test?

<p>cherry red</p> Signup and view all the answers

The enzyme __________ removes the amine group from phenylalanine during deamination.

<p>phenylalanine deaminase</p> Signup and view all the answers

Match the type of test with its purpose:

<p>Indole Test = Detects the production of indole from tryptophan Phenylalanine Deaminase Test = Detects phenylpyruvic acid Methyl Red Test = Determines stable acid production from glucose Voges-Proskauer Test = Detects acetoin production</p> Signup and view all the answers

What is produced when phenylalanine is deaminated?

<p>Phenylpyruvic acid (B)</p> Signup and view all the answers

The Voges-Proskauer test detects the immediate production of CO2 during glucose fermentation.

<p>False (B)</p> Signup and view all the answers

What reagent is used to test for phenylpyruvic acid in the phenylalanine test?

<p>ferric chloride</p> Signup and view all the answers

After the methyl red reagent is added to the MR test, a __________ color indicates a positive result.

<p>red</p> Signup and view all the answers

Match the bacteria with the tests they are used in:

<p>Escherichia coli = MR and VP tests Enterobacter aerogenes = MR and VP tests Specific species = Indole and phenylalanine tests</p> Signup and view all the answers

What indicates a positive test result in the phenylalanine deaminase test?

<p>Dark green color (B)</p> Signup and view all the answers

It is essential to read the results of the phenylalanine test immediately after it is performed.

<p>True (A)</p> Signup and view all the answers

What is the role of Kovac's Reagent in the indole test?

<p>To detect indole production</p> Signup and view all the answers

The MRVP tests assess the fermentation of __________.

<p>glucose</p> Signup and view all the answers

What color indicates positive glucose fermentation in the Enterotube II results?

<p>Yellow (C)</p> Signup and view all the answers

Gas production in Enterotube II is indicated by the wax being lifted.

<p>True (A)</p> Signup and view all the answers

What would you circle on the results sheet for positive lactose fermentation?

<p>2</p> Signup and view all the answers

A positive result for H2S production in the Enterotube II results in a _______ color.

<p>Black</p> Signup and view all the answers

Match the Enterotube II tests with their positive result colors:

<p>Glucose fermentation = Yellow Urea hydrolysis = Red or purple Lysine decarboxylase = Purple Citrate = Blue</p> Signup and view all the answers

Which test requires separate testing and is not included in the Enterotube II?

<p>Indole production (B)</p> Signup and view all the answers

What color indicates a positive VP test result?

<p>Red (C)</p> Signup and view all the answers

A negative nitrate reduction test indicates that nitrate was indeed reduced to nitrite.

<p>False (B)</p> Signup and view all the answers

The Enterotube II can provide a five-digit reference number directly after testing.

<p>False (B)</p> Signup and view all the answers

What is the possible code for Salmonella spp. indicated in the text?

<p>6704752</p> Signup and view all the answers

What is the main purpose of the nitrate reduction test?

<p>To determine the reduction of nitrate to nitrite in a broth culture.</p> Signup and view all the answers

If no red color appears after adding zinc dust in a nitrate reduction test, the test is considered _____

<p>positive</p> Signup and view all the answers

The substrate tested for carbohydrate fermentation in Enterotube II includes _______.

<p>Glucose</p> Signup and view all the answers

Which substrate is NOT included in the Enterotube II's biochemical components?

<p>Sucrose (A)</p> Signup and view all the answers

What type of environment is recommended for the incubation of the API 20e strip?

<p>35°C (B)</p> Signup and view all the answers

The Voges-Proskauer test is part of the Enterotube II.

<p>True (A)</p> Signup and view all the answers

The Enterotube II can be inoculated from multiple colonies on an agar plate.

<p>False (B)</p> Signup and view all the answers

In the Enterotube II, what does a negative result for dulcitol fermentation indicate?

<p>Any other color</p> Signup and view all the answers

What happens if a red color is observed after adding nitrate A and nitrate B solutions?

<p>The test is positive for nitrite presence.</p> Signup and view all the answers

To find the identification of a microorganism using Enterotube II, the user must locate the _______ number.

<p>5-digit</p> Signup and view all the answers

The API-20e test is primarily used for identifying _____ shaped bacteria.

<p>rod</p> Signup and view all the answers

Match the following components of the Enterotube II with their functions:

<p>Glucose = Sugar fermentation Indole = Tryptophan degradation Urea = Hydrolysis of urea Citrate = Utilization of citrate as a carbon source</p> Signup and view all the answers

What is the positive result for PA deaminase in the Enterotube II?

<p>Black or smoky gray (D)</p> Signup and view all the answers

What temperature should the nitrate broth be incubated at?

<p>37°C (C)</p> Signup and view all the answers

Zinc dust is used to catalyze the reduction of nitrite back to nitrate.

<p>False (B)</p> Signup and view all the answers

What role does zinc dust play in the nitrate reduction test?

<p>It catalyzes the reduction of any unreduced nitrate to nitrite.</p> Signup and view all the answers

The _____ test is used to identify E. coli using the Enterotube II system.

<p>procedure</p> Signup and view all the answers

Which of the following tests uses a profile number to identify bacterial species?

<p>API-20e test (D)</p> Signup and view all the answers

Enterobacteriaceae are characterized by being gram-positive bacteria.

<p>False (B)</p> Signup and view all the answers

What is the primary enzyme responsible for lipid hydrolysis in the spirit blue agar assay?

<p>Lipase (D)</p> Signup and view all the answers

Starch is small enough to pass through the bacterial cell membrane without hydrolysis.

<p>False (B)</p> Signup and view all the answers

What color does iodine turn when it complexes with starch?

<p>blue to dark brown</p> Signup and view all the answers

The enzyme that breaks down casein in milk is called _____ .

<p>casease</p> Signup and view all the answers

Match the bacterial species with the assay they are used in:

<p>Staphylococcus aureus = Spirit blue agar Escherichia coli = Starch hydrolysis Bacillus cereus = Starch hydrolysis</p> Signup and view all the answers

What indicates a positive result in the starch hydrolysis test?

<p>Clear colorless zone around the bacterial growth (C)</p> Signup and view all the answers

The spirit blue dye serves as a nutritional component for bacteria in spirit blue agar.

<p>False (B)</p> Signup and view all the answers

What environmental condition is required for incubation of the plates?

<p>37°C</p> Signup and view all the answers

In the lipase assay, the presence of a clear halo indicates the _____ of tributyrin.

<p>hydrolysis</p> Signup and view all the answers

Match the type of agar with its associated test:

<p>Spirit blue agar = Lipid hydrolysis Starch agar = Starch hydrolysis Skim milk agar = Casein hydrolysis Gelatin agar = Gelatinase production</p> Signup and view all the answers

What happens if bacterial species do not produce casease on skim milk agar?

<p>They will not produce a clear zone. (D)</p> Signup and view all the answers

The hydrolysis of starch is detectable without using an indicator.

<p>False (B)</p> Signup and view all the answers

Which two enzymes are involved in starch hydrolysis?

<p>Amylase and oligo-1,6-glucosidase</p> Signup and view all the answers

The presence of _____ positive bacteria in spirit blue agar is indicated by a clear halo around colonies.

<p>lipase</p> Signup and view all the answers

Which of the following bacteria can contribute to the development of dental caries?

<p>All of the above (D)</p> Signup and view all the answers

Streptococcus pyogenes is responsible for diseases primarily in the throat.

<p>True (A)</p> Signup and view all the answers

What type of streptococci is characterized by producing a transparent zone on blood agar?

<p>β-Hemolytic streptococci</p> Signup and view all the answers

The _____ test is used to differentiate group A and B beta hemolytic streptococci.

<p>bacitracin</p> Signup and view all the answers

Match the following bacteria with their characteristics:

<p>Streptococcus pneumoniae = Gram-positive, lancet-shaped cocci S. agalactiae = Causes neonatal pneumonia Viridans streptococci = Leading cause of subacute bacterial endocarditis S. pyogenes = Responsible for strep throat</p> Signup and view all the answers

Which laboratory test is used to identify Streptococcus pneumoniae?

<p>Optochin sensitivity (C)</p> Signup and view all the answers

Viridans streptococci are primarily associated with causing infections in the gastrointestinal tract.

<p>True (A)</p> Signup and view all the answers

What is the group associated with Streptococcus agalactiae?

<p>Group B</p> Signup and view all the answers

Lancefield groups are used to classify streptococci based on their cell wall _____ ability.

<p>antigen</p> Signup and view all the answers

Match the following bacteria with the diseases they cause:

<p>Staphylococcus epidermidis = Catheter-related infections S. pneumoniae = Community-acquired pneumonia S. pyogenes = Strep throat S. agalactiae = Neonatal meningitis</p> Signup and view all the answers

Which of the following types of streptococci form dark green colonies on blood agar?

<p>α-Hemolytic streptococci (A)</p> Signup and view all the answers

Staphylococcus aureus is typically resistant to antibiotics.

<p>False (B)</p> Signup and view all the answers

What are the characteristics of Staphylococci?

<p>Gram-positive cocci, catalase positive, facultatively anaerobic.</p> Signup and view all the answers

Invasive infections by S. pneumoniae may include _____ and septicemia.

<p>bacteremia</p> Signup and view all the answers

Which Staphylococcus species is known for causing acute urinary tract infections in young women?

<p>Staphylococcus saprophyticus (D)</p> Signup and view all the answers

Staphylococcus aureus can produce toxins that have harmful effects on the host.

<p>True (A)</p> Signup and view all the answers

What is the main disease associated with Staphylococcus aureus enterotoxin?

<p>gastroenteritis</p> Signup and view all the answers

Staphylococcus saprophyticus is ________ in coagulase tests.

<p>negative</p> Signup and view all the answers

Match the Staphylococcus species with their key characteristics:

<p>Staphylococcus epidermidis = Negative coagulase test, sensitive to novobiocin Staphylococcus saprophyticus = Negative coagulase test, resistant to novobiocin Staphylococcus aureus = Positive coagulase test, sensitive to novobiocin</p> Signup and view all the answers

Which characteristic distinguishes MRSA from other Staphylococcus species?

<p>Resistant to methicillin (B)</p> Signup and view all the answers

Staphylococcus saprophyticus is part of the normal flora.

<p>False (B)</p> Signup and view all the answers

What symptoms are commonly associated with toxic shock syndrome caused by Staphylococcus aureus?

<p>fever, rash, hypotension, and shock</p> Signup and view all the answers

Staphylococcus aureus is sensitive to low doses of ________.

<p>novobiocin</p> Signup and view all the answers

A 22-year-old female with painful urination tested positive for catalase and was resistant to novobiocin. What organism is most likely causing her symptoms?

<p>Staphylococcus saprophyticus (D)</p> Signup and view all the answers

Staphylococcus aureus can cause food intoxication through enterotoxins.

<p>True (A)</p> Signup and view all the answers

What is the hemolytic reaction of Staphylococcus aureus on blood agar?

<p>beta-hemolytic</p> Signup and view all the answers

The Gram stain of a sputum sample produced ________ that were catalase negative and formed diplococci.

<p>gram-positive cocci</p> Signup and view all the answers

Match the case with the likely pathogen:

<p>Case 1 = Streptococcus pneumoniae Case 2 = Staphylococcus aureus Case 3 = Staphylococcus saprophyticus Case 4 = Group B Streptococcus</p> Signup and view all the answers

What is the primary mechanism of disease for Staphylococcus aureus associated with food contamination?

<p>Food intoxication (D)</p> Signup and view all the answers

What pathogen is most commonly associated with urinary tract infections (UTIs)?

<p>Escherichia coli (D)</p> Signup and view all the answers

Gram-positive cocci in chains indicate the presence of Streptococcus species.

<p>True (A)</p> Signup and view all the answers

What is the primary cause of diarrhea and colitis in individuals infected with CDIFF?

<p>Toxin A and B (C)</p> Signup and view all the answers

Infant botulism can occur if newborns are fed honey.

<p>True (A)</p> Signup and view all the answers

What are the typical symptoms of a urinary tract infection?

<p>Dysuria</p> Signup and view all the answers

The procedure to test urine includes a dip stick that reveals the presence of nitrites, white blood cells, and ______.

<p>blood</p> Signup and view all the answers

What is the typical symptom of tetanus caused by Clostridium tetani?

<p>Lockjaw</p> Signup and view all the answers

Clostridium perfringens often causes a condition known as _____ gangrene.

<p>Gas</p> Signup and view all the answers

Match the bacteria to their potential classification or condition:

<p>Escherichia coli = Most common UTI pathogen Klebsiella = Less common UTI pathogen Streptococcus = Gram-positive cocci in chains Staphylococcus = Gram-positive cocci in clusters</p> Signup and view all the answers

Match the types of botulism with their descriptions:

<p>Food botulism = Related to home canned foods Wound botulism = Infections in contaminated wounds Infant botulism = Caused by feeding honey to infants Equine botulism = Primary treatment involves horse antitoxins</p> Signup and view all the answers

Which Gram stain result indicates a respiratory infection caused by Streptococcus pneumoniae?

<p>Gram-positive diplococci (B)</p> Signup and view all the answers

Which antibiotics can replace the current treatment for Clostridium difficile infection?

<p>Vancomycin (C)</p> Signup and view all the answers

A productive cough is characterized by coughing up sputum.

<p>True (A)</p> Signup and view all the answers

What is the purpose of using a candle jar in throat culture procedures?

<p>Create a microaerophilic environment</p> Signup and view all the answers

Clostridium botulinum toxins primarily affect the digestive system.

<p>False (B)</p> Signup and view all the answers

The process of examining sputum for white blood cells aims to identify potential __________.

<p>infection</p> Signup and view all the answers

What is the common source of contamination associated with Clostridium perfringens food intoxication?

<p>Contaminated meat</p> Signup and view all the answers

Match the urinary symptoms with their definitions:

<p>Dysuria = Painful urination Hematuria = Blood in urine Nocturia = Frequent nighttime urination Polyuria = Excessive urination</p> Signup and view all the answers

The DTaP vaccine is required for children in the _____ to control tetanus infections.

<p>U.S.</p> Signup and view all the answers

What should be used to read the dip-stick test results?

<p>A color chart (C)</p> Signup and view all the answers

The presence of β-hemolytic zones on blood agar indicates non-invasive infections.

<p>False (B)</p> Signup and view all the answers

Which of the following is NOT a symptom of pneumonia?

<p>Diarrhea (B)</p> Signup and view all the answers

Which of the following is a symptom of botulism?

<p>Double vision (A)</p> Signup and view all the answers

What is the significance of finding more than 100,000 CFU/mL in a quantitative urine test?

<p>It indicates a urinary tract infection (UTI).</p> Signup and view all the answers

The main method by which intestinal bacteria can cause urinary tract infections is through improper hygiene.

<p>True (A)</p> Signup and view all the answers

To conduct a qualitative test of urine samples, use _____ agar and _____ agar.

<p>blood; MacConkey</p> Signup and view all the answers

What is the goal of performing a Gram stain on a throat culture?

<p>Identify the type of bacteria present</p> Signup and view all the answers

Match the bacterial pathogens with their associated conditions:

<p>Clostridium difficile = Diarrhea and colitis Clostridium tetani = Tetanus Clostridium botulinum = Botulism Clostridium perfringens = Food poisoning</p> Signup and view all the answers

The presence of beta-hemolytic zones on culture indicates the possibility of __________ infection.

<p>streptococcal</p> Signup and view all the answers

After incubation, what should you check on the blood agar for throat culture?

<p>Colony morphology (B)</p> Signup and view all the answers

What is the purpose of streak plating urine samples onto agar plates?

<p>To isolate and quantify bacteria (D)</p> Signup and view all the answers

It is acceptable to shake the dip-stick before use.

<p>False (B)</p> Signup and view all the answers

How does E. coli appear on MacConkey agar?

<p>As pink colonies indicating lactose fermentation.</p> Signup and view all the answers

The dilution factor when using a sterile loop that carries 0.001 mL of urine is _____.

<p>1000</p> Signup and view all the answers

Which antibiotic is commonly associated with CDIFF infections?

<p>Ampicillin (C)</p> Signup and view all the answers

Urine samples with 120 colonies would indicate a urinary tract infection.

<p>False (B)</p> Signup and view all the answers

What is the incubation temperature for agar plates after streaking?

<p>35°C</p> Signup and view all the answers

CDIFF is often associated with _____ following extensive antibiotic use.

<p>diarrhea</p> Signup and view all the answers

What is recorded in Table 20.1?

<p>Results of the dip-stick test (C)</p> Signup and view all the answers

Match the following tables with their purpose:

<p>Table 20.1 = Dip-stick test results Table 20.2 = Qualitative test results Table 20.3 = Quantitative test results</p> Signup and view all the answers

Flashcards

Sulfate Reduction

The process where microorganisms metabolize sulfate salts or sulfur-containing amino acids, producing hydrogen sulfide (H2S).

Hydrogen Sulfide (H2S)

A gas produced during sulfate reduction in anaerobic conditions.

SIM Tube

A tube containing semi-solid agar medium used for three tests: sulfate reduction, motility, and indole production.

Motility Test

A test to determine if bacteria can move.

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Indole Production

The metabolic breakdown of the amino acid tryptophan, producing indole.

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Kovac's Reagent

A chemical used to detect the presence of indole.

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Nitrate Reduction Test

A test used to determine if bacteria can reduce nitrates to nitrites or nitrogen gas.

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Bacterial Motility

The ability of bacteria to move from one location to another.

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Stab inoculation

Inserting a needle into a culture media, commonly used to test bacterial motility and hydrogen sulfide production

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Hydrogen Sulfide production

A process where certain bacteria produce hydrogen sulfide gas as a byproduct of their metabolism.

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Indole test

A biochemical test used to detect the production of indole by bacteria.

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Phenylalanine deaminase test

A test to determine if a bacterium produces the enzyme phenylalanine deaminase.

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Deamination

The removal of an amino group from an amino acid.

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Phenylalanine agar

A medium used in the phenylalanine deaminase test.

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Ferric chloride reagent

A chemical used to detect phenylpyruvic acid.

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Methyl Red test

A test identifying if a bacteria produces acid end products from glucose.

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Voges-Proskauer test

Detects acetoin production during glucose fermentation.

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MRVP tubes

Broths used in both Methyl Red and Voges-Proskauer tests

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Mixed acid fermentation

A type of glucose fermentation that produces various acids and potentially gas.

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Acetoin

An intermediate compound in certain bacterial fermentations.

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Diacetyl

A compound formed from oxidation of acetoin, producing a positive result in a Voges-Proskauer test.

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Glucose fermentation

Process of bacteria breaking down glucose.

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Positive Nitrate Reduction

Indicates the bacteria have enzymes to reduce nitrate to nitrite, which can be detected by adding reagents. A red color indicates a positive result.

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Negative Nitrate Reduction

Can mean the nitrate was not reduced, or it was reduced to another form, like nitrogen gas (N2). Zinc dust is used to determine if nitrate was not reduced.

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Zinc Dust in Nitrate Reduction

Zinc dust is added to the culture to catalyze the reduction of any remaining nitrate to nitrite. If a red color appears, the test is negative because the nitrate wasn't reduced by the bacteria.

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Methyl Red Test (MR)

Tests for the production of mixed acids (e.g., lactic, acetic, formic) during the fermentation of glucose. A red color indicates a positive result.

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Positive MR Test

Indicates high acid production during glucose fermentation, likely due to the production of mixed acids. This often applies to certain types of bacteria like E. coli.

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VP Test (Voges-Proskauer)

Tests for the production of acetoin (a precursor to diacetyl), another byproduct of glucose fermentation. A red color indicates a positive result.

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Positive VP Test

Suggests the bacteria can produce acetoin, which is later oxidized to diacetyl. Enterobacter species usually show a positive VP reaction.

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Enterotube II

A rapid, multi-component test system used to identify bacteria belonging to the Enterobacteriaceae family. It contains 12 compartments with various biochemical substrates for testing.

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API-20e

A test strip with 20 compartments containing dehydrated substrates used to identify enteric gram-negative rods. It allows rapid identification based on color changes and profile analysis.

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Enterobacteriaceae

A family of gram-negative, rod-shaped bacteria that are often found in the intestines of humans and animals.

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MacConkey Agar

A selective and differential medium used to isolate and differentiate members of the Enterobacteriaceae.

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XLT-4 Agar

A selective and diagnostic medium used for the isolation of Salmonella from food samples and clinical specimens.

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Lipase Test

A test that uses spirit blue agar to detect the presence of lipase, an enzyme that breaks down fats.

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Tributyrin Oil

A common type of fat used in lipase tests. It forms a light blue complex with spirit blue dye.

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Spirit Blue Agar

A medium used in lipase tests that contains tributyrin oil and spirit blue dye. Lipase positive bacteria will create a clear halo around their colonies.

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Starch Hydrolysis

The breakdown of starch into simpler sugars by bacterial enzymes.

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Amylase

An enzyme that breaks down starch into smaller units.

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Oligo-1,6-glucosidase

An enzyme that breaks down branching units of starch.

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Protein Hydrolysis (Protease Test)

A test that determines if bacteria can break down proteins by producing the enzyme protease.

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Casein

A protein found in milk that can be broken down by the enzyme casease.

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Casease

An enzyme that breaks down casein, the protein in milk.

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Skim Milk Agar

A medium used to test for casein hydrolysis. It contains skim milk, which is a good source of casein.

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Zone of Clearing

A clear area around a bacterial colony on skim milk agar, indicating casein hydrolysis.

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Positive Hydrolysis

Indicates that the bacteria are able to break down the substrate, such as starch or casein.

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Negative Hydrolysis

Indicates that the bacteria are unable to break down the substrate.

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Extracellular Enzymatic Activities

The production of enzymes by bacteria that are released outside of the cell to break down substances in the surrounding environment.

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Lysine Decarboxylase

The enzyme that removes the carboxyl group from lysine, producing cadaverine. A positive result is indicated by a purple color change in the medium.

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H2S Production

The production of hydrogen sulfide (H2S) gas, often indicated by a black precipitate. A positive result suggests the bacteria can use sulfur-containing compounds.

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Adonitol Fermentation

The ability of bacteria to ferment the sugar adonitol. A positive result is indicated by a yellow color change in the medium.

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Arabinose Fermentation

The ability of bacteria to ferment the sugar arabinose. A positive result is indicated by a yellow color change in the medium.

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Sorbitol Fermentation

The ability of bacteria to ferment the sugar sorbitol. A positive result is indicated by a yellow color change in the medium.

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Dulcitol Fermentation

The ability of bacteria to ferment the sugar dulcitol. A positive result is indicated by a yellow color change in the medium.

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PA Deaminase

The enzyme that removes the amino group from phenylalanine, producing phenylpyruvic acid. A positive result is indicated by the formation of a black or smoky gray precipitate.

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Urea Hydrolysis

The ability of bacteria to break down urea into ammonia and carbon dioxide. A positive result is indicated by a pink or red color change in the medium.

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Citrate Utilization

The ability of bacteria to use citrate as a carbon source. A positive result is indicated by a blue color change in the medium.

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Lancefield Groups

A classification system grouping streptococci based on their cell wall antigens. Group A (S. pyogenes), Group B (S. agalactiae), and Group D (Enterococcus spp.) are clinically significant.

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α-Hemolytic Streptococci

Streptococci that partially break down red blood cells in blood agar, resulting in a greenish discoloration. Examples include Streptococcus pneumoniae and Viridans Streptococcus.

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Optochin Sensitivity

A test used to differentiate Streptococcus pneumoniae from other α-hemolytic streptococci. S. pneumoniae is sensitive to optochin, showing a zone of inhibition around the disc.

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Streptococcus pneumoniae Characteristics

Gram-positive, lancet-shaped diplococci (pairs) that cause pneumonia, otitis media (ear infections), bacteremia, and septicemia.

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Viridans Streptococci Characteristics

Resident bacteria in the mouth and gut, producing a greenish color on blood agar. Examples: S. bovis, S. mitis, S. oralis, and others. Can cause endocarditis.

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β-Hemolytic Streptococci

Streptococci that completely destroy red blood cells in blood agar, creating a clear zone. Examples include S. pyogenes (Group A) and S. agalactiae (Group B).

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Bacitracin Sensitivity

A test used to differentiate Group A (S. pyogenes) from other β-hemolytic streptococci. Group A is sensitive to bacitracin.

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Streptococcus pyogenes Characteristics

Gram-positive cocci in chains. A major human pathogen causing strep throat, tonsillitis, pharyngitis, and skin infections.

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Streptococcus agalactiae Characteristics

A major cause of neonatal infections, including pneumonia, septicemia, and meningitis in newborns.

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Staphylococci Characteristics

Gram-positive cocci, arranged in grape-like clusters. Commonly found on skin and in the upper respiratory tract.

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Staphylococcus epidermidis Characteristics

The most common staphylococcus species found on human skin and mucous membranes.

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Opportunistic Pathogens

Microorganisms that are normally harmless but can cause disease under certain conditions, like when the host's immune system is weakened.

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Dip-stick test

A rapid test using a strip to detect presence of substances like white blood cells, nitrites, or blood in urine.

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Qualitative test

A test to determine if a substance is present or absent. It doesn't provide the exact amount.

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β-hemolytic zone

A clear zone around bacterial colonies on blood agar plates, indicating the bacteria produce an enzyme that breaks down red blood cells.

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Quantitative test

Measures the amount of a substance, giving a numerical value.

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Bacterial cell count

A count of the number of bacteria in a sample, typically expressed as colony-forming units (CFU) per milliliter (mL).

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Urinary tract infection (UTI)

An infection in the urinary tract, often caused by bacteria.

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Colony-forming unit (CFU)

A single bacterium or a group of bacteria that can grow and form a visible colony on an agar plate.

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Sterile loop

A loop sterilized by heat or other methods that is used to transfer bacteria or other microorganisms.

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Streak plating

A method for separating bacteria on an agar plate by spreading a sample across its surface.

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Incubation

The process of growing bacteria at a specific temperature and for a specific duration.

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Nosocomial infection

An infection acquired in a hospital or healthcare setting.

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Clostridium difficile

A bacterium that can cause severe diarrhea and colitis, especially after antibiotic use.

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Antibiotic-associated colitis

Inflammation of the colon that occurs after taking antibiotics.

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Pseudomembranous colitis

A severe form of antibiotic-associated colitis characterized by plaque-like lesions in the colon.

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Staphylococcus epidermidis

A common skin bacterium, often found as part of the normal flora, but can cause infections in individuals with weakened immune systems or those with invasive medical devices.

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Staphylococcus saprophyticus

A bacterium associated with urinary tract infections, particularly in young, sexually active women.

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Staphylococcus aureus

A bacterium known for its ability to cause various infections, including food poisoning, skin infections, and more serious conditions like toxic shock syndrome.

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What are the three main mechanisms of human disease caused by S. aureus?

The three main mechanisms are food intoxication, toxic shock syndrome, and MRSA.

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Food Intoxication

An illness caused by consuming food contaminated with S. aureus enterotoxin, which causes severe gastroenteritis.

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Toxic Shock Syndrome (TSS)

A serious, life-threatening condition caused by a toxin produced by S. aureus, which can lead to fever, rash, hypotension, and shock.

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Methicillin-Resistant Staphylococcus aureus (MRSA)

A strain of S. aureus that is resistant to many antibiotics, including methicillin, making it difficult to treat.

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What are the characteristics that help differentiate Staphylococcus species?

Characteristics include Gram-stain results, hemolytic reaction, catalase test, mannitol salt agar test, coagulase test, and reaction to novobiocin.

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Alpha hemolytic

Describes bacteria that partially break down red blood cells, creating a green zone around their colonies on blood agar.

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Catalase Test

A test used to identify bacteria that produce the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen.

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Coagulase Test

A test used to identify bacteria that produce the enzyme coagulase, which causes the clotting of blood plasma.

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Novobiocin

An antibiotic used to differentiate Staphylococcus species based on their sensitivity or resistance.

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Case study 1: What pathogen is likely causing John's mother's respiratory symptoms?

Streptococcus pneumoniae is likely causing John's mother's respiratory symptoms based on the provided information.

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Case study 2: What organism is most likely causing the 27-year-old female's symptoms?

Staphylococcus aureus is the most likely culprit causing the 27-year-old female's symptoms, particularly given the history of using a contraceptive sponge and development of a rash and hypotension.

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Case study 3: What organism is causing the 22-year-old female's dysuria?

Staphylococcus saprophyticus is causing the 22-year-old female's dysuria, as it is known for causing UTIs in young women.

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Case study 4: What pathogen is most likely causing the 5-day-old baby's sepsis?

Streptococcus agalactiae (Group B Streptococcus) is the most likely pathogen causing the 5-day-old baby's sepsis, given its age and the presence of beta-hemolytic, catalase-negative cocci resistant to bacitracin.

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What is a UTI?

A urinary tract infection (UTI) is an infection of the urinary tract, typically caused by bacteria. It often involves the bladder and urethra, but can also affect the kidneys.

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What are the common symptoms of a UTI?

Common symptoms of a UTI include dysuria (painful urination), frequent urination, urgency to urinate, blood in the urine, and cloudy or foul-smelling urine.

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What causes UTIs?

UTIs are usually caused by bacteria that normally live in the digestive tract. These bacteria can enter the urethra and travel to the bladder, leading to infection.

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How is a UTI diagnosed?

A UTI can be diagnosed through a urine test, where a doctor checks for the presence of bacteria, white blood cells, and blood. A dip-stick test can also be used to detect nitrites and leukocyte esterase.

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What is a dip-stick test for UTI?

A urine dip-stick test is a quick and easy way to check for signs of UTI. It detects nitrites (produced by bacteria), leukocyte esterase (an enzyme from white blood cells), and blood in the urine.

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Why are nitrites present in the urine during a UTI?

Certain bacteria, like E. coli, in the urinary tract can convert nitrates (present in urine) into nitrites. This conversion is a sign of a UTI.

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Why are leukocyte esterase and white blood cells present in UTI?

When there's a UTI, your body sends white blood cells (immune cells) to fight the infection. These cells release leukocyte esterase, an enzyme that can be detected in urine.

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What is Escherichia coli (E. coli) in the context of UTI?

E. coli is a common bacterium found in the gut. It's the most frequent cause of UTIs, often accounting for 80% of cases. It can sometimes release enzymes that break down red blood cells, creating a beta-hemolytic zone on a blood agar plate.

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What are some alternative causes of UTI besides E. coli?

Besides E. coli, other bacteria like Klebsiella, Enterobacter, Serratia, and Proteus can also cause UTIs.

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What are some ways to prevent UTIs?

You can help prevent UTIs by peeing when you feel the need, wiping front to back after using the bathroom, staying well-hydrated, and avoiding holding your pee for too long.

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What is a 'causal catch' urine sample?

A 'causal catch' urine sample is a urine specimen collected for testing, typically for UTI diagnosis, that's taken immediately after urination, without any specific preparation beforehand.

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How does 'holding on' affect UTI risk?

Holding your pee for too long can increase the risk of UTI by allowing bacteria that have entered the urethra to have more time to ascend into the bladder.

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What is a catheter and how is it related to UTIs?

A catheter is a tube inserted into the bladder to help drain urine. Leaving a catheter in place for a long time without changing it increases the risk of UTI because bacteria can travel up the catheter and into the bladder.

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How does 'bloodstream' affect UTI?

In some cases, bacteria may travel through the bloodstream and lodge in the kidneys, leading to a more serious type of UTI called pyelonephritis.

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What is the major bacteria causing UTIs?

Escherichia coli (E. coli) is the most common bacteria causing UTIs, accounting for about 80% of cases.

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Clostridium difficile (C. diff)

A bacterium that colonizes the gut and releases toxins A and B, causing diarrhea and inflammation of the large intestine (colitis).

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C. diff Toxins

Toxins A and B released by C. difficile, which bind to receptors in the colon's lining, triggering inflammation and diarrhea.

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C. diff Treatment

Treatments include consuming lactic acid-rich products like yogurt, fecal transplants, or replacing antibiotics with vancomycin.

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Clostridium botulinum

A bacterium that produces neurotoxins, primarily A, B, and E, causing neuroparalytic diseases like botulism.

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Botulism Symptoms

Typical symptoms include double vision, difficulty speaking and swallowing, and muscle weakness.

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Botulism Types

The three types are food botulism from low-acid canned foods, wound botulism from infected wounds, and infant botulism from honey consumption.

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Clostridium tetani

A bacterium causing tetanus, characterized by muscle spasms and stiffness, commonly called 'lockjaw'.

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Tetanus Symptoms

Muscle contractions without relaxation (tetany) leading to lockjaw and muscle spasms throughout the body, sometimes fatal.

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Clostridium perfringens

A bacterium responsible for gas gangrene, a serious infection causing tissue damage and gas production in wounds or dead tissue.

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Gas Gangrene Symptoms

Gas gangrene starts in anaerobic areas like wounds and dead tissue. It spreads rapidly, causing tissue damage, gas production, and potentially septic shock.

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Study Notes

Clostridium Species Overview

  • Clostridium species are gram-positive, rod-shaped bacteria.
  • They are obligately anaerobic and spore-forming.
  • Several species cause various diseases, including intestinal issues.

Clostridium Difficile

  • A common nosocomial infection.
  • Causes colitis and diarrhea, often after antibiotic use.
  • Symptoms include severe colon inflammation and black water diarrhea.
  • Produces toxins A and B that damage colon cells.
  • Treatment options include yogurt, fecal transplants, and antibiotic replacement.

Clostridium Botulinum

  • Produces toxins (A, B, E) linked to neuroparalytic diseases.
  • Food botulism is related to low-acid, home-canned foods.
  • Wound botulism from contaminated wounds.
  • Infant botulism—linked to feeding honey to infants.
  • Treatment includes equine antitoxins and antibiotics.

Clostridium Tetani

  • Causes "lockjaw" (muscle spasms).
  • Infection usually from wounds, burns, or ulcers.
  • Tetanospasmin toxin is released by germinating spores.
  • Death can result from breathing muscle interference.
  • DTaP vaccination is a primary prevention method.

Clostridium Perfringens

  • β-Hemolytic double zones on blood agar.
  • Five serotypes linked to diverse toxins.
  • Causes gas gangrene (tissue damage, gas production).
  • Food intoxication associated with contaminated meats (low temp/long time cooking).
  • Symptoms include diarrhea, cramps, and abdominal pain.
  • Food safety measures include thorough cooking of poultry/meat products

Klebsiella pneumoniae

  • Gram-negative rod shape bacteria.
  • Possesses a heavy capsule.
  • Causes severe pneumonia.

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This quiz focuses on the Sulfate Indole Motility (SIM) test, a crucial procedure in microbiology for understanding bacterial characteristics. You will explore aspects like hydrogen sulfide production, indole visualization, and the interpretation of test results. Test your knowledge on procedures, bacterial traits, and biochemical reactions involved in the SIM test.

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