Podcast
Questions and Answers
What does the sulfate indole motility (SIM) tube test for?
What does the sulfate indole motility (SIM) tube test for?
- Sulfate reduction, indole production, and motility (correct)
- Amino acid deamination, hydrogen sulfide, and fermentation
- Fermentation, nitrate reduction, and motility
- Protein synthesis, nitrate reduction, and indole production
The production of hydrogen sulfide (H2S) in a SIM test is an aerobic process.
The production of hydrogen sulfide (H2S) in a SIM test is an aerobic process.
False (B)
What reagent must be added to a culture tube to visualize indole production?
What reagent must be added to a culture tube to visualize indole production?
Kovac’s reagent
The _____ produced when sulfate salts are metabolized will form a BLACK insoluble metal sulfide precipitate.
The _____ produced when sulfate salts are metabolized will form a BLACK insoluble metal sulfide precipitate.
Which of the following procedures is NOT part of the sulfate indole motility test?
Which of the following procedures is NOT part of the sulfate indole motility test?
Match the bacteria with their common characteristics:
Match the bacteria with their common characteristics:
Indole is created from the breakdown of the amino acid lysine.
Indole is created from the breakdown of the amino acid lysine.
In the motility test using SIM media, how can you determine motility?
In the motility test using SIM media, how can you determine motility?
What temperature should the bacteria be incubated at during the tests?
What temperature should the bacteria be incubated at during the tests?
A yellow color in the phenylalanine deaminase test indicates a positive result.
A yellow color in the phenylalanine deaminase test indicates a positive result.
What color change indicates a positive result for the indole test?
What color change indicates a positive result for the indole test?
The enzyme __________ removes the amine group from phenylalanine during deamination.
The enzyme __________ removes the amine group from phenylalanine during deamination.
Match the type of test with its purpose:
Match the type of test with its purpose:
What is produced when phenylalanine is deaminated?
What is produced when phenylalanine is deaminated?
The Voges-Proskauer test detects the immediate production of CO2 during glucose fermentation.
The Voges-Proskauer test detects the immediate production of CO2 during glucose fermentation.
What reagent is used to test for phenylpyruvic acid in the phenylalanine test?
What reagent is used to test for phenylpyruvic acid in the phenylalanine test?
After the methyl red reagent is added to the MR test, a __________ color indicates a positive result.
After the methyl red reagent is added to the MR test, a __________ color indicates a positive result.
Match the bacteria with the tests they are used in:
Match the bacteria with the tests they are used in:
What indicates a positive test result in the phenylalanine deaminase test?
What indicates a positive test result in the phenylalanine deaminase test?
It is essential to read the results of the phenylalanine test immediately after it is performed.
It is essential to read the results of the phenylalanine test immediately after it is performed.
What is the role of Kovac's Reagent in the indole test?
What is the role of Kovac's Reagent in the indole test?
The MRVP tests assess the fermentation of __________.
The MRVP tests assess the fermentation of __________.
What color indicates positive glucose fermentation in the Enterotube II results?
What color indicates positive glucose fermentation in the Enterotube II results?
Gas production in Enterotube II is indicated by the wax being lifted.
Gas production in Enterotube II is indicated by the wax being lifted.
What would you circle on the results sheet for positive lactose fermentation?
What would you circle on the results sheet for positive lactose fermentation?
A positive result for H2S production in the Enterotube II results in a _______ color.
A positive result for H2S production in the Enterotube II results in a _______ color.
Match the Enterotube II tests with their positive result colors:
Match the Enterotube II tests with their positive result colors:
Which test requires separate testing and is not included in the Enterotube II?
Which test requires separate testing and is not included in the Enterotube II?
What color indicates a positive VP test result?
What color indicates a positive VP test result?
A negative nitrate reduction test indicates that nitrate was indeed reduced to nitrite.
A negative nitrate reduction test indicates that nitrate was indeed reduced to nitrite.
The Enterotube II can provide a five-digit reference number directly after testing.
The Enterotube II can provide a five-digit reference number directly after testing.
What is the possible code for Salmonella spp. indicated in the text?
What is the possible code for Salmonella spp. indicated in the text?
What is the main purpose of the nitrate reduction test?
What is the main purpose of the nitrate reduction test?
If no red color appears after adding zinc dust in a nitrate reduction test, the test is considered _____
If no red color appears after adding zinc dust in a nitrate reduction test, the test is considered _____
The substrate tested for carbohydrate fermentation in Enterotube II includes _______.
The substrate tested for carbohydrate fermentation in Enterotube II includes _______.
Which substrate is NOT included in the Enterotube II's biochemical components?
Which substrate is NOT included in the Enterotube II's biochemical components?
What type of environment is recommended for the incubation of the API 20e strip?
What type of environment is recommended for the incubation of the API 20e strip?
The Voges-Proskauer test is part of the Enterotube II.
The Voges-Proskauer test is part of the Enterotube II.
The Enterotube II can be inoculated from multiple colonies on an agar plate.
The Enterotube II can be inoculated from multiple colonies on an agar plate.
In the Enterotube II, what does a negative result for dulcitol fermentation indicate?
In the Enterotube II, what does a negative result for dulcitol fermentation indicate?
What happens if a red color is observed after adding nitrate A and nitrate B solutions?
What happens if a red color is observed after adding nitrate A and nitrate B solutions?
To find the identification of a microorganism using Enterotube II, the user must locate the _______ number.
To find the identification of a microorganism using Enterotube II, the user must locate the _______ number.
The API-20e test is primarily used for identifying _____ shaped bacteria.
The API-20e test is primarily used for identifying _____ shaped bacteria.
Match the following components of the Enterotube II with their functions:
Match the following components of the Enterotube II with their functions:
What is the positive result for PA deaminase in the Enterotube II?
What is the positive result for PA deaminase in the Enterotube II?
What temperature should the nitrate broth be incubated at?
What temperature should the nitrate broth be incubated at?
Zinc dust is used to catalyze the reduction of nitrite back to nitrate.
Zinc dust is used to catalyze the reduction of nitrite back to nitrate.
What role does zinc dust play in the nitrate reduction test?
What role does zinc dust play in the nitrate reduction test?
The _____ test is used to identify E. coli using the Enterotube II system.
The _____ test is used to identify E. coli using the Enterotube II system.
Which of the following tests uses a profile number to identify bacterial species?
Which of the following tests uses a profile number to identify bacterial species?
Enterobacteriaceae are characterized by being gram-positive bacteria.
Enterobacteriaceae are characterized by being gram-positive bacteria.
What is the primary enzyme responsible for lipid hydrolysis in the spirit blue agar assay?
What is the primary enzyme responsible for lipid hydrolysis in the spirit blue agar assay?
Starch is small enough to pass through the bacterial cell membrane without hydrolysis.
Starch is small enough to pass through the bacterial cell membrane without hydrolysis.
What color does iodine turn when it complexes with starch?
What color does iodine turn when it complexes with starch?
The enzyme that breaks down casein in milk is called _____ .
The enzyme that breaks down casein in milk is called _____ .
Match the bacterial species with the assay they are used in:
Match the bacterial species with the assay they are used in:
What indicates a positive result in the starch hydrolysis test?
What indicates a positive result in the starch hydrolysis test?
The spirit blue dye serves as a nutritional component for bacteria in spirit blue agar.
The spirit blue dye serves as a nutritional component for bacteria in spirit blue agar.
What environmental condition is required for incubation of the plates?
What environmental condition is required for incubation of the plates?
In the lipase assay, the presence of a clear halo indicates the _____ of tributyrin.
In the lipase assay, the presence of a clear halo indicates the _____ of tributyrin.
Match the type of agar with its associated test:
Match the type of agar with its associated test:
What happens if bacterial species do not produce casease on skim milk agar?
What happens if bacterial species do not produce casease on skim milk agar?
The hydrolysis of starch is detectable without using an indicator.
The hydrolysis of starch is detectable without using an indicator.
Which two enzymes are involved in starch hydrolysis?
Which two enzymes are involved in starch hydrolysis?
The presence of _____ positive bacteria in spirit blue agar is indicated by a clear halo around colonies.
The presence of _____ positive bacteria in spirit blue agar is indicated by a clear halo around colonies.
Which of the following bacteria can contribute to the development of dental caries?
Which of the following bacteria can contribute to the development of dental caries?
Streptococcus pyogenes is responsible for diseases primarily in the throat.
Streptococcus pyogenes is responsible for diseases primarily in the throat.
What type of streptococci is characterized by producing a transparent zone on blood agar?
What type of streptococci is characterized by producing a transparent zone on blood agar?
The _____ test is used to differentiate group A and B beta hemolytic streptococci.
The _____ test is used to differentiate group A and B beta hemolytic streptococci.
Match the following bacteria with their characteristics:
Match the following bacteria with their characteristics:
Which laboratory test is used to identify Streptococcus pneumoniae?
Which laboratory test is used to identify Streptococcus pneumoniae?
Viridans streptococci are primarily associated with causing infections in the gastrointestinal tract.
Viridans streptococci are primarily associated with causing infections in the gastrointestinal tract.
What is the group associated with Streptococcus agalactiae?
What is the group associated with Streptococcus agalactiae?
Lancefield groups are used to classify streptococci based on their cell wall _____ ability.
Lancefield groups are used to classify streptococci based on their cell wall _____ ability.
Match the following bacteria with the diseases they cause:
Match the following bacteria with the diseases they cause:
Which of the following types of streptococci form dark green colonies on blood agar?
Which of the following types of streptococci form dark green colonies on blood agar?
Staphylococcus aureus is typically resistant to antibiotics.
Staphylococcus aureus is typically resistant to antibiotics.
What are the characteristics of Staphylococci?
What are the characteristics of Staphylococci?
Invasive infections by S. pneumoniae may include _____ and septicemia.
Invasive infections by S. pneumoniae may include _____ and septicemia.
Which Staphylococcus species is known for causing acute urinary tract infections in young women?
Which Staphylococcus species is known for causing acute urinary tract infections in young women?
Staphylococcus aureus can produce toxins that have harmful effects on the host.
Staphylococcus aureus can produce toxins that have harmful effects on the host.
What is the main disease associated with Staphylococcus aureus enterotoxin?
What is the main disease associated with Staphylococcus aureus enterotoxin?
Staphylococcus saprophyticus is ________ in coagulase tests.
Staphylococcus saprophyticus is ________ in coagulase tests.
Match the Staphylococcus species with their key characteristics:
Match the Staphylococcus species with their key characteristics:
Which characteristic distinguishes MRSA from other Staphylococcus species?
Which characteristic distinguishes MRSA from other Staphylococcus species?
Staphylococcus saprophyticus is part of the normal flora.
Staphylococcus saprophyticus is part of the normal flora.
What symptoms are commonly associated with toxic shock syndrome caused by Staphylococcus aureus?
What symptoms are commonly associated with toxic shock syndrome caused by Staphylococcus aureus?
Staphylococcus aureus is sensitive to low doses of ________.
Staphylococcus aureus is sensitive to low doses of ________.
A 22-year-old female with painful urination tested positive for catalase and was resistant to novobiocin. What organism is most likely causing her symptoms?
A 22-year-old female with painful urination tested positive for catalase and was resistant to novobiocin. What organism is most likely causing her symptoms?
Staphylococcus aureus can cause food intoxication through enterotoxins.
Staphylococcus aureus can cause food intoxication through enterotoxins.
What is the hemolytic reaction of Staphylococcus aureus on blood agar?
What is the hemolytic reaction of Staphylococcus aureus on blood agar?
The Gram stain of a sputum sample produced ________ that were catalase negative and formed diplococci.
The Gram stain of a sputum sample produced ________ that were catalase negative and formed diplococci.
Match the case with the likely pathogen:
Match the case with the likely pathogen:
What is the primary mechanism of disease for Staphylococcus aureus associated with food contamination?
What is the primary mechanism of disease for Staphylococcus aureus associated with food contamination?
What pathogen is most commonly associated with urinary tract infections (UTIs)?
What pathogen is most commonly associated with urinary tract infections (UTIs)?
Gram-positive cocci in chains indicate the presence of Streptococcus species.
Gram-positive cocci in chains indicate the presence of Streptococcus species.
What is the primary cause of diarrhea and colitis in individuals infected with CDIFF?
What is the primary cause of diarrhea and colitis in individuals infected with CDIFF?
Infant botulism can occur if newborns are fed honey.
Infant botulism can occur if newborns are fed honey.
What are the typical symptoms of a urinary tract infection?
What are the typical symptoms of a urinary tract infection?
The procedure to test urine includes a dip stick that reveals the presence of nitrites, white blood cells, and ______.
The procedure to test urine includes a dip stick that reveals the presence of nitrites, white blood cells, and ______.
What is the typical symptom of tetanus caused by Clostridium tetani?
What is the typical symptom of tetanus caused by Clostridium tetani?
Clostridium perfringens often causes a condition known as _____ gangrene.
Clostridium perfringens often causes a condition known as _____ gangrene.
Match the bacteria to their potential classification or condition:
Match the bacteria to their potential classification or condition:
Match the types of botulism with their descriptions:
Match the types of botulism with their descriptions:
Which Gram stain result indicates a respiratory infection caused by Streptococcus pneumoniae?
Which Gram stain result indicates a respiratory infection caused by Streptococcus pneumoniae?
Which antibiotics can replace the current treatment for Clostridium difficile infection?
Which antibiotics can replace the current treatment for Clostridium difficile infection?
A productive cough is characterized by coughing up sputum.
A productive cough is characterized by coughing up sputum.
What is the purpose of using a candle jar in throat culture procedures?
What is the purpose of using a candle jar in throat culture procedures?
Clostridium botulinum toxins primarily affect the digestive system.
Clostridium botulinum toxins primarily affect the digestive system.
The process of examining sputum for white blood cells aims to identify potential __________.
The process of examining sputum for white blood cells aims to identify potential __________.
What is the common source of contamination associated with Clostridium perfringens food intoxication?
What is the common source of contamination associated with Clostridium perfringens food intoxication?
Match the urinary symptoms with their definitions:
Match the urinary symptoms with their definitions:
The DTaP vaccine is required for children in the _____ to control tetanus infections.
The DTaP vaccine is required for children in the _____ to control tetanus infections.
What should be used to read the dip-stick test results?
What should be used to read the dip-stick test results?
The presence of β-hemolytic zones on blood agar indicates non-invasive infections.
The presence of β-hemolytic zones on blood agar indicates non-invasive infections.
Which of the following is NOT a symptom of pneumonia?
Which of the following is NOT a symptom of pneumonia?
Which of the following is a symptom of botulism?
Which of the following is a symptom of botulism?
What is the significance of finding more than 100,000 CFU/mL in a quantitative urine test?
What is the significance of finding more than 100,000 CFU/mL in a quantitative urine test?
The main method by which intestinal bacteria can cause urinary tract infections is through improper hygiene.
The main method by which intestinal bacteria can cause urinary tract infections is through improper hygiene.
To conduct a qualitative test of urine samples, use _____ agar and _____ agar.
To conduct a qualitative test of urine samples, use _____ agar and _____ agar.
What is the goal of performing a Gram stain on a throat culture?
What is the goal of performing a Gram stain on a throat culture?
Match the bacterial pathogens with their associated conditions:
Match the bacterial pathogens with their associated conditions:
The presence of beta-hemolytic zones on culture indicates the possibility of __________ infection.
The presence of beta-hemolytic zones on culture indicates the possibility of __________ infection.
After incubation, what should you check on the blood agar for throat culture?
After incubation, what should you check on the blood agar for throat culture?
What is the purpose of streak plating urine samples onto agar plates?
What is the purpose of streak plating urine samples onto agar plates?
It is acceptable to shake the dip-stick before use.
It is acceptable to shake the dip-stick before use.
How does E. coli appear on MacConkey agar?
How does E. coli appear on MacConkey agar?
The dilution factor when using a sterile loop that carries 0.001 mL of urine is _____.
The dilution factor when using a sterile loop that carries 0.001 mL of urine is _____.
Which antibiotic is commonly associated with CDIFF infections?
Which antibiotic is commonly associated with CDIFF infections?
Urine samples with 120 colonies would indicate a urinary tract infection.
Urine samples with 120 colonies would indicate a urinary tract infection.
What is the incubation temperature for agar plates after streaking?
What is the incubation temperature for agar plates after streaking?
CDIFF is often associated with _____ following extensive antibiotic use.
CDIFF is often associated with _____ following extensive antibiotic use.
What is recorded in Table 20.1?
What is recorded in Table 20.1?
Match the following tables with their purpose:
Match the following tables with their purpose:
Flashcards
Sulfate Reduction
Sulfate Reduction
The process where microorganisms metabolize sulfate salts or sulfur-containing amino acids, producing hydrogen sulfide (H2S).
Hydrogen Sulfide (H2S)
Hydrogen Sulfide (H2S)
A gas produced during sulfate reduction in anaerobic conditions.
SIM Tube
SIM Tube
A tube containing semi-solid agar medium used for three tests: sulfate reduction, motility, and indole production.
Motility Test
Motility Test
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Indole Production
Indole Production
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Kovac's Reagent
Kovac's Reagent
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Nitrate Reduction Test
Nitrate Reduction Test
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Bacterial Motility
Bacterial Motility
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Stab inoculation
Stab inoculation
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Hydrogen Sulfide production
Hydrogen Sulfide production
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Indole test
Indole test
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Phenylalanine deaminase test
Phenylalanine deaminase test
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Deamination
Deamination
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Phenylalanine agar
Phenylalanine agar
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Ferric chloride reagent
Ferric chloride reagent
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Methyl Red test
Methyl Red test
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Voges-Proskauer test
Voges-Proskauer test
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MRVP tubes
MRVP tubes
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Mixed acid fermentation
Mixed acid fermentation
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Acetoin
Acetoin
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Diacetyl
Diacetyl
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Glucose fermentation
Glucose fermentation
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Positive Nitrate Reduction
Positive Nitrate Reduction
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Negative Nitrate Reduction
Negative Nitrate Reduction
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Zinc Dust in Nitrate Reduction
Zinc Dust in Nitrate Reduction
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Methyl Red Test (MR)
Methyl Red Test (MR)
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Positive MR Test
Positive MR Test
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VP Test (Voges-Proskauer)
VP Test (Voges-Proskauer)
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Positive VP Test
Positive VP Test
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Enterotube II
Enterotube II
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API-20e
API-20e
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Enterobacteriaceae
Enterobacteriaceae
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MacConkey Agar
MacConkey Agar
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XLT-4 Agar
XLT-4 Agar
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Lipase Test
Lipase Test
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Tributyrin Oil
Tributyrin Oil
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Spirit Blue Agar
Spirit Blue Agar
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Starch Hydrolysis
Starch Hydrolysis
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Amylase
Amylase
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Oligo-1,6-glucosidase
Oligo-1,6-glucosidase
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Protein Hydrolysis (Protease Test)
Protein Hydrolysis (Protease Test)
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Casein
Casein
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Casease
Casease
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Skim Milk Agar
Skim Milk Agar
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Zone of Clearing
Zone of Clearing
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Positive Hydrolysis
Positive Hydrolysis
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Negative Hydrolysis
Negative Hydrolysis
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Extracellular Enzymatic Activities
Extracellular Enzymatic Activities
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Lysine Decarboxylase
Lysine Decarboxylase
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H2S Production
H2S Production
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Adonitol Fermentation
Adonitol Fermentation
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Arabinose Fermentation
Arabinose Fermentation
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Sorbitol Fermentation
Sorbitol Fermentation
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Dulcitol Fermentation
Dulcitol Fermentation
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PA Deaminase
PA Deaminase
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Urea Hydrolysis
Urea Hydrolysis
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Citrate Utilization
Citrate Utilization
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Lancefield Groups
Lancefield Groups
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α-Hemolytic Streptococci
α-Hemolytic Streptococci
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Optochin Sensitivity
Optochin Sensitivity
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Streptococcus pneumoniae Characteristics
Streptococcus pneumoniae Characteristics
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Viridans Streptococci Characteristics
Viridans Streptococci Characteristics
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β-Hemolytic Streptococci
β-Hemolytic Streptococci
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Bacitracin Sensitivity
Bacitracin Sensitivity
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Streptococcus pyogenes Characteristics
Streptococcus pyogenes Characteristics
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Streptococcus agalactiae Characteristics
Streptococcus agalactiae Characteristics
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Staphylococci Characteristics
Staphylococci Characteristics
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Staphylococcus epidermidis Characteristics
Staphylococcus epidermidis Characteristics
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Opportunistic Pathogens
Opportunistic Pathogens
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Dip-stick test
Dip-stick test
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Qualitative test
Qualitative test
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β-hemolytic zone
β-hemolytic zone
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Quantitative test
Quantitative test
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Bacterial cell count
Bacterial cell count
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Urinary tract infection (UTI)
Urinary tract infection (UTI)
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Colony-forming unit (CFU)
Colony-forming unit (CFU)
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Sterile loop
Sterile loop
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Streak plating
Streak plating
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Incubation
Incubation
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Nosocomial infection
Nosocomial infection
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Clostridium difficile
Clostridium difficile
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Antibiotic-associated colitis
Antibiotic-associated colitis
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Pseudomembranous colitis
Pseudomembranous colitis
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Staphylococcus epidermidis
Staphylococcus epidermidis
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Staphylococcus saprophyticus
Staphylococcus saprophyticus
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Staphylococcus aureus
Staphylococcus aureus
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What are the three main mechanisms of human disease caused by S. aureus?
What are the three main mechanisms of human disease caused by S. aureus?
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Food Intoxication
Food Intoxication
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Toxic Shock Syndrome (TSS)
Toxic Shock Syndrome (TSS)
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Methicillin-Resistant Staphylococcus aureus (MRSA)
Methicillin-Resistant Staphylococcus aureus (MRSA)
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What are the characteristics that help differentiate Staphylococcus species?
What are the characteristics that help differentiate Staphylococcus species?
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Alpha hemolytic
Alpha hemolytic
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Catalase Test
Catalase Test
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Coagulase Test
Coagulase Test
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Novobiocin
Novobiocin
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Case study 1: What pathogen is likely causing John's mother's respiratory symptoms?
Case study 1: What pathogen is likely causing John's mother's respiratory symptoms?
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Case study 2: What organism is most likely causing the 27-year-old female's symptoms?
Case study 2: What organism is most likely causing the 27-year-old female's symptoms?
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Case study 3: What organism is causing the 22-year-old female's dysuria?
Case study 3: What organism is causing the 22-year-old female's dysuria?
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Case study 4: What pathogen is most likely causing the 5-day-old baby's sepsis?
Case study 4: What pathogen is most likely causing the 5-day-old baby's sepsis?
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What is a UTI?
What is a UTI?
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What are the common symptoms of a UTI?
What are the common symptoms of a UTI?
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What causes UTIs?
What causes UTIs?
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How is a UTI diagnosed?
How is a UTI diagnosed?
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What is a dip-stick test for UTI?
What is a dip-stick test for UTI?
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Why are nitrites present in the urine during a UTI?
Why are nitrites present in the urine during a UTI?
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Why are leukocyte esterase and white blood cells present in UTI?
Why are leukocyte esterase and white blood cells present in UTI?
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What is Escherichia coli (E. coli) in the context of UTI?
What is Escherichia coli (E. coli) in the context of UTI?
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What are some alternative causes of UTI besides E. coli?
What are some alternative causes of UTI besides E. coli?
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What are some ways to prevent UTIs?
What are some ways to prevent UTIs?
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What is a 'causal catch' urine sample?
What is a 'causal catch' urine sample?
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How does 'holding on' affect UTI risk?
How does 'holding on' affect UTI risk?
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What is a catheter and how is it related to UTIs?
What is a catheter and how is it related to UTIs?
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How does 'bloodstream' affect UTI?
How does 'bloodstream' affect UTI?
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What is the major bacteria causing UTIs?
What is the major bacteria causing UTIs?
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Clostridium difficile (C. diff)
Clostridium difficile (C. diff)
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C. diff Toxins
C. diff Toxins
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C. diff Treatment
C. diff Treatment
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Clostridium botulinum
Clostridium botulinum
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Botulism Symptoms
Botulism Symptoms
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Botulism Types
Botulism Types
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Clostridium tetani
Clostridium tetani
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Tetanus Symptoms
Tetanus Symptoms
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Clostridium perfringens
Clostridium perfringens
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Gas Gangrene Symptoms
Gas Gangrene Symptoms
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Study Notes
Clostridium Species Overview
- Clostridium species are gram-positive, rod-shaped bacteria.
- They are obligately anaerobic and spore-forming.
- Several species cause various diseases, including intestinal issues.
Clostridium Difficile
- A common nosocomial infection.
- Causes colitis and diarrhea, often after antibiotic use.
- Symptoms include severe colon inflammation and black water diarrhea.
- Produces toxins A and B that damage colon cells.
- Treatment options include yogurt, fecal transplants, and antibiotic replacement.
Clostridium Botulinum
- Produces toxins (A, B, E) linked to neuroparalytic diseases.
- Food botulism is related to low-acid, home-canned foods.
- Wound botulism from contaminated wounds.
- Infant botulism—linked to feeding honey to infants.
- Treatment includes equine antitoxins and antibiotics.
Clostridium Tetani
- Causes "lockjaw" (muscle spasms).
- Infection usually from wounds, burns, or ulcers.
- Tetanospasmin toxin is released by germinating spores.
- Death can result from breathing muscle interference.
- DTaP vaccination is a primary prevention method.
Clostridium Perfringens
- β-Hemolytic double zones on blood agar.
- Five serotypes linked to diverse toxins.
- Causes gas gangrene (tissue damage, gas production).
- Food intoxication associated with contaminated meats (low temp/long time cooking).
- Symptoms include diarrhea, cramps, and abdominal pain.
- Food safety measures include thorough cooking of poultry/meat products
Klebsiella pneumoniae
- Gram-negative rod shape bacteria.
- Possesses a heavy capsule.
- Causes severe pneumonia.
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Description
This quiz focuses on the Sulfate Indole Motility (SIM) test, a crucial procedure in microbiology for understanding bacterial characteristics. You will explore aspects like hydrogen sulfide production, indole visualization, and the interpretation of test results. Test your knowledge on procedures, bacterial traits, and biochemical reactions involved in the SIM test.