Microbiology Quiz on Pathogens and Techniques
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Questions and Answers

What is the primary role of the M2 protein in Influenza A?

  • Facilitates viral entry into host cells
  • Stabilizes the viral envelope
  • Inhibits viral replication
  • Acidifies the interior of the viral particle (correct)
  • Which mechanism is primarily targeted by quinolones like nalidixic acid in bacterial cells?

  • RNA polymerase
  • DNA gyrase (correct)
  • Ribosomes
  • Cell wall synthesis
  • In the context of bacterial vaginosis, what are clue cells?

  • Highly differentiated immune cells
  • Cells indicative of normal flora
  • Vaginal epithelial cells coated with bacteria (correct)
  • Immune cells attacking pathogens
  • What is the mechanism by which sulfonamides exert their antimicrobial effect?

    <p>Compete with PABA for binding</p> Signup and view all the answers

    How does the A subunit of Vibrio cholerae’s AB toxin affect host cells?

    <p>Activates Gs protein to increase cAMP</p> Signup and view all the answers

    Which organism is specifically known to grow as yellow colonies on TCBS agar?

    <p>Vibrio cholerae</p> Signup and view all the answers

    What method do many viruses use to enter host cells?

    <p>Endocytosis</p> Signup and view all the answers

    Which of the following is most often associated with catheter-related bloodstream infections?

    <p>Staphylococcus epidermidis</p> Signup and view all the answers

    Study Notes

    Sulfonamides Mechanism of Action

    • Sulfonamides inhibit folic acid synthesis by competing with para-aminobenzoic acid (PABA).

    Vibrio cholerae Identification

    • Vibrio cholerae forms yellow colonies on TCBS agar.

    Influenza A M2 Protein

    • The M2 protein acidifies the viral particle's interior, facilitating viral uncoating.

    Healthcare-Associated Infections (HAIs) Causes

    • Poor hand hygiene and use of invasive devices (e.g., catheters, ventilators) are common causes.

    Bacterial Vaginosis (BV) Microscopic Diagnosis

    • Clue cells (vaginal epithelial cells coated with bacteria) are indicative of BV.

    Enrichment Media in Microbiology

    • Enrichment media promote the growth of specific microorganisms, while inhibiting others.

    Fungal Detection in Air Samples

    • Sabouraud Dextrose Agar (SDA) is commonly used to detect fungi in environmental air samples.

    Vibrio cholerae AB Toxin (A subunit)

    • The A subunit of the Vibrio cholerae AB toxin activates the Gs protein, increasing cAMP levels and causing fluid and electrolyte loss.

    Quinolones Target

    • Quinolones, such as nalidixic acid, target bacterial DNA gyrase, preventing DNA replication.

    Viral Entry into Host Cells

    • One method of viral entry is endocytosis, where the virus is engulfed by the host cell into a vesicle.

    HIV Reverse Transcriptase Function

    • HIV reverse transcriptase converts viral RNA into DNA for integration into the host genome.

    Vibrio cholerae Yellow Colonies on TCBS Agar

    • Vibrio cholerae ferments sucrose, producing acidic byproducts that cause the TCBS agar to turn yellow.

    Clue Cells and Condition

    • Clue cells are vaginal epithelial cells coated with bacteria and are associated with bacterial vaginosis (BV).

    Influenza A Neuraminidase (NA) Function

    • Neuraminidase (NA) releases progeny virions from the host cell by cleaving sialic acid residues.
    • Staphylococcus epidermidis (a coagulase-negative Staphylococcus) is a common pathogen causing catheter-related bloodstream infections.

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    Description

    Test your knowledge on key microbiological concepts, including the mechanisms of action of sulfonamides, identification of Vibrio cholerae, and the role of M2 protein in influenza A. Also covered are healthcare-associated infections, bacterial vaginosis diagnosis, and methods of fungal detection. Perfect for students of microbiology and healthcare.

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