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Questions and Answers
What method is used to confirm a diagnosis of diphtheria?
What method is used to confirm a diagnosis of diphtheria?
- Detection of 'phage plagues' in cultures of suspicious isolates
- Isolation of a typical colony on Tinsdale's agar (correct)
- Microscopic appearance of organisms stained with methylene blue
- Isolation of typical organisms from materials such as blood, showing invasiveness
Listeria monocytogenes has which notable characteristic?
Listeria monocytogenes has which notable characteristic?
- It is an extracellular pathogen
- It is a gram-negative coccus
- It is catalase-negative
- It can grow at refrigerator temperatures (4°C) (correct)
Which one of the following does N.meningitidis exhibit, but not N.gonorrhoeae?
Which one of the following does N.meningitidis exhibit, but not N.gonorrhoeae?
- Gram-negative cocci
- Ferments maltose (correct)
- Ferments glucose
- Oxidase positive
Which characteristic is NOT associated with Listeria monocytogenes?
Which characteristic is NOT associated with Listeria monocytogenes?
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Which method confirms the presence of toxin production by a suspicious isolate in diphtheria diagnosis?
Which method confirms the presence of toxin production by a suspicious isolate in diphtheria diagnosis?
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Study Notes
Diagnostic Methods for Diphtheria
- A diagnosis of diphtheria is confirmed by microscopic examination of organisms stained with methylene blue
- Isolation of a typical colony on Tinsdale's agar is another method of confirmation
- Invasive nature of the disease can be demonstrated by isolating typical organisms from materials such as blood
- Detection of "phage plagues" in cultures of suspicious isolates is also a diagnostic method
- Demonstration of toxin production by a suspicious isolate is a key diagnostic feature
Characteristics of Listeria monocytogenes
- Listeria monocytogenes can grow at refrigerator temperatures (4°C)
- It is an intracellular pathogen, not an extracellular one
- The bacterium is catalase-positive
- Listeria monocytogenes is a gram-positive rod, not a coccus
- It is a zoonotic pathogen, not strictly a human pathogen
Characteristics of Neisseria meningitidis
- Neisseria meningitidis ferments glucose, a characteristic that distinguishes it from Neisseria gonorrhoeae
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