Microbiology Quiz
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Questions and Answers

What method is used to confirm a diagnosis of diphtheria?

  • Detection of 'phage plagues' in cultures of suspicious isolates
  • Isolation of a typical colony on Tinsdale's agar (correct)
  • Microscopic appearance of organisms stained with methylene blue
  • Isolation of typical organisms from materials such as blood, showing invasiveness
  • Listeria monocytogenes has which notable characteristic?

  • It is an extracellular pathogen
  • It is a gram-negative coccus
  • It is catalase-negative
  • It can grow at refrigerator temperatures (4°C) (correct)
  • Which one of the following does N.meningitidis exhibit, but not N.gonorrhoeae?

  • Gram-negative cocci
  • Ferments maltose (correct)
  • Ferments glucose
  • Oxidase positive
  • Which characteristic is NOT associated with Listeria monocytogenes?

    <p>It is catalase-negative</p> Signup and view all the answers

    Which method confirms the presence of toxin production by a suspicious isolate in diphtheria diagnosis?

    <p>Demonstration of toxin production by a suspicious isolate</p> Signup and view all the answers

    Study Notes

    Diagnostic Methods for Diphtheria

    • A diagnosis of diphtheria is confirmed by microscopic examination of organisms stained with methylene blue
    • Isolation of a typical colony on Tinsdale's agar is another method of confirmation
    • Invasive nature of the disease can be demonstrated by isolating typical organisms from materials such as blood
    • Detection of "phage plagues" in cultures of suspicious isolates is also a diagnostic method
    • Demonstration of toxin production by a suspicious isolate is a key diagnostic feature

    Characteristics of Listeria monocytogenes

    • Listeria monocytogenes can grow at refrigerator temperatures (4°C)
    • It is an intracellular pathogen, not an extracellular one
    • The bacterium is catalase-positive
    • Listeria monocytogenes is a gram-positive rod, not a coccus
    • It is a zoonotic pathogen, not strictly a human pathogen

    Characteristics of Neisseria meningitidis

    • Neisseria meningitidis ferments glucose, a characteristic that distinguishes it from Neisseria gonorrhoeae

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    Test your knowledge of microbiology with these questions on diagnosing bacterial infections, including diphtheria and Listeria monocytogenes.

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