Microbiology Quiz: Bacterial Diseases and Laboratory Techniques

Which one of the following organisms is least likely to cause non-bloody diarrhea for the past 14 hours?

The class of an immunoglobulin is determined by:

Immunological memory is predominantly the function of which of the following?

All of the following are true with respect to IgM antibodies EXCEPT which one?

The cells of the adaptive immune system are T- and B-cell lymphocytes. Both are:

Which technique is used to isolate a single type of microorganism from a mixed culture?

Which of the following is NOT made using a pure culture?

What is the primary purpose of using a pure culture in microbial experiments?

Diarrhea Causes

A 30-year-old woman with non-bloody diarrhea for 14 hours is unlikely to be caused by Streptococcus pyogens.
Clostridium difficile, Shigella dysenteriae, and Salmonella enteritidis are more likely causes of diarrhea.

Microbiology Techniques

Pure culture: a culture that contains only one type of microorganism, important for studying the characteristics of a specific microbe.
Spread plates: a type of plate used to isolate and count microorganisms, prepared by spreading a small volume of culture onto the surface of the plate.
Streak plates: a type of plate used to isolate microorganisms, prepared by streaking a small volume of culture onto the surface of the plate in a specific pattern.
Pour plates: a type of plate used to count microorganisms, prepared by pouring a small volume of culture into a molten agar medium.

Immunology

The class of an immunoglobulin is determined by the heavy chain type.
Immunological memory is predominantly the function of cells of the adaptive immune system.
IgM antibodies:
fix complement
occur on the surface of lymphocytes
predominate in the primary response to antigen
are glycoproteins
do not mediate allergic reactions
T- and B-cell lymphocytes are antigen-specific, long-lived cells with "memory" for the antigen.

This quiz tests your knowledge of bacterial diseases and laboratory techniques used to isolate and identify microorganisms. Questions cover the causative agents of diarrhea and the preparation of pure cultures.