Klebsiella and Pseudomonas Practical Microbiology Lab
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Questions and Answers

1- What are the important properties of Klebsiella bacteria?

1- Gram-negative, oxidase-negative, rod-shaped bacteria, non-motile. 2- They tend to be shorter and thicker when compared to others in the family Enterobacteriaceae. 3- Found in the large intestine but are also present in soil and water. 4- Has a very large polysaccharide capsule. 5- The species are aerobic but facultatively anaerobic.

3- What are the important properties of Pseudomonas bacteria?

1- A large group of strictly aerobic, non-sporing gram-negative bacteria motile by polar flagella. 2- Produce water-soluble pigments. 3- Opportunistic pathogens. P. aeruginosa produces two pigments useful in clinical and laboratory diagnosis: (1) pyocyanin, which can color the pus in a wound blue, and (2) pyoverdin (fluorescein), a yellow-green pigment that fluoresces under ultraviolet light.

2- What kind of colonies do Klebsiella organisms produce on differential agar? What are the distinguishing features of these organisms?

Organisms of this group produce lactose-fermenting (pink colored) colonies on differential agar such as MacConkey’s or EMB. These organisms are differentiated by the use of biochemical tests.

4- What pigments are produced by Pseudomonas aeruginosa and how are they useful in clinical and laboratory diagnosis?

<p>P. aeruginosa produces two pigments useful in clinical and laboratory diagnosis: (1) pyocyanin, which can color the pus in a wound blue, and (2) pyoverdin (fluorescein), a yellow-green pigment that fluoresces under ultraviolet light.</p> Signup and view all the answers

5- What kind of infections are usually caused by Klebsiella organisms?

<p>These organisms are usually opportunistic pathogens that cause nosocomial infections, especially pneumonia and urinary tract infections. K. pneumoniae is an important respiratory tract pathogen outside hospitals as well.</p> Signup and view all the answers

Study Notes

Properties of Klebsiella Bacteria

  • Klebsiella bacteria have important properties that distinguish them from other microorganisms.

Colony Characteristics of Klebsiella

  • Klebsiella organisms produce mucoid colonies on differential agar.
  • The distinguishing features of these colonies are their large, moist, and mucoid appearance.

Properties of Pseudomonas Bacteria

  • Pseudomonas bacteria have distinct properties that set them apart from other bacteria.

Pigments Produced by Pseudomonas Aeruginosa

  • Pseudomonas aeruginosa produces two types of pigments: pyocyanin and pyoverdin.
  • These pigments are useful in clinical and laboratory diagnosis, as they help identify Pseudomonas aeruginosa infections.

Infections Caused by Klebsiella Organisms

  • Klebsiella organisms typically cause hospital-acquired infections, such as urinary tract infections, respiratory infections, and surgical wound infections.

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Test your knowledge of Klebsiella and Pseudomonas with this practical microbiology lab quiz. Learn about the important properties and diseases caused by these gram-negative, oxidase-negative, rod-shaped bacteria.

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