Klebsiella and Pseudomonas Practical Microbiology Lab

1- Gram-negative, oxidase-negative, rod-shaped bacteria, non-motile. 2- They tend to be shorter and thicker when compared to others in the family Enterobacteriaceae. 3- Found in the large intestine but are also present in soil and water. 4- Has a very large polysaccharide capsule. 5- The species are aerobic but facultatively anaerobic.

1- A large group of strictly aerobic, non-sporing gram-negative bacteria motile by polar flagella. 2- Produce water-soluble pigments. 3- Opportunistic pathogens. P. aeruginosa produces two pigments useful in clinical and laboratory diagnosis: (1) pyocyanin, which can color the pus in a wound blue, and (2) pyoverdin (fluorescein), a yellow-green pigment that fluoresces under ultraviolet light.

Organisms of this group produce lactose-fermenting (pink colored) colonies on differential agar such as MacConkey’s or EMB. These organisms are differentiated by the use of biochemical tests.

<p>P. aeruginosa produces two pigments useful in clinical and laboratory diagnosis: (1) pyocyanin, which can color the pus in a wound blue, and (2) pyoverdin (fluorescein), a yellow-green pigment that fluoresces under ultraviolet light.</p> Signup and view all the answers

<p>These organisms are usually opportunistic pathogens that cause nosocomial infections, especially pneumonia and urinary tract infections. K. pneumoniae is an important respiratory tract pathogen outside hospitals as well.</p> Signup and view all the answers