Klebsiella and Pseudomonas Practical Microbiology Lab

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Questions and Answers

1- What are the important properties of Klebsiella bacteria?

1- Gram-negative, oxidase-negative, rod-shaped bacteria, non-motile. 2- They tend to be shorter and thicker when compared to others in the family Enterobacteriaceae. 3- Found in the large intestine but are also present in soil and water. 4- Has a very large polysaccharide capsule. 5- The species are aerobic but facultatively anaerobic.

3- What are the important properties of Pseudomonas bacteria?

1- A large group of strictly aerobic, non-sporing gram-negative bacteria motile by polar flagella. 2- Produce water-soluble pigments. 3- Opportunistic pathogens. P. aeruginosa produces two pigments useful in clinical and laboratory diagnosis: (1) pyocyanin, which can color the pus in a wound blue, and (2) pyoverdin (fluorescein), a yellow-green pigment that fluoresces under ultraviolet light.

2- What kind of colonies do Klebsiella organisms produce on differential agar? What are the distinguishing features of these organisms?

Organisms of this group produce lactose-fermenting (pink colored) colonies on differential agar such as MacConkey’s or EMB. These organisms are differentiated by the use of biochemical tests.

4- What pigments are produced by Pseudomonas aeruginosa and how are they useful in clinical and laboratory diagnosis?

<p>P. aeruginosa produces two pigments useful in clinical and laboratory diagnosis: (1) pyocyanin, which can color the pus in a wound blue, and (2) pyoverdin (fluorescein), a yellow-green pigment that fluoresces under ultraviolet light.</p> Signup and view all the answers

5- What kind of infections are usually caused by Klebsiella organisms?

<p>These organisms are usually opportunistic pathogens that cause nosocomial infections, especially pneumonia and urinary tract infections. K. pneumoniae is an important respiratory tract pathogen outside hospitals as well.</p> Signup and view all the answers

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Study Notes

Properties of Klebsiella Bacteria

Klebsiella bacteria have important properties that distinguish them from other microorganisms.

Colony Characteristics of Klebsiella

Klebsiella organisms produce mucoid colonies on differential agar.
The distinguishing features of these colonies are their large, moist, and mucoid appearance.

Properties of Pseudomonas Bacteria

Pseudomonas bacteria have distinct properties that set them apart from other bacteria.

Pigments Produced by Pseudomonas Aeruginosa

Pseudomonas aeruginosa produces two types of pigments: pyocyanin and pyoverdin.
These pigments are useful in clinical and laboratory diagnosis, as they help identify Pseudomonas aeruginosa infections.

Infections Caused by Klebsiella Organisms

Klebsiella organisms typically cause hospital-acquired infections, such as urinary tract infections, respiratory infections, and surgical wound infections.