Microbiology Overview and Classification

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Questions and Answers

Which of the following is an example of a fomite?

  • A contaminated needle (correct)
  • A coughing person
  • An infected animal
  • A mosquito

Which of the following is a characteristic of airborne transmission?

  • The pathogen is transmitted through direct contact.
  • The pathogen travels less than a meter.
  • The pathogen is transmitted through contaminated food.
  • The pathogen travels more than 1 meter. (correct)

What is the term for bacteria that live in a healthy person without causing harm but can become pathogens under certain conditions?

  • Commensal bacteria
  • Transient flora
  • Virulent bacteria
  • Opportunistic pathogens (correct)

Which of the following is NOT a mode of transmission for infectious agents?

<p>Genetic inheritance (B)</p> Signup and view all the answers

Which of the following diseases is NOT typically transmitted through contaminated food or water?

<p>Measles (A)</p> Signup and view all the answers

What type of vector carries pathogens passively on their body parts?

<p>Mechanical vector (A)</p> Signup and view all the answers

Which of the following is an example of an application of applied microbiology in the medical field?

<p>Development of antibiotics (D)</p> Signup and view all the answers

Which of the following is an example of a transient flora?

<p>Bacillus laterosporus (B)</p> Signup and view all the answers

Which of the following is a major factor that can disrupt the balance of normal flora and lead to opportunistic infections?

<p>Weakened immune system (B)</p> Signup and view all the answers

What term describes the transmission of a pathogen through direct physical contact from one person to another?

<p>Direct contact transmission (B)</p> Signup and view all the answers

Which of these organisms are classified as prokaryotes?

<p>Bacteria (C)</p> Signup and view all the answers

Which branch of microbiology focuses on the study of how microorganisms impact human health?

<p>Public health microbiology (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic of a bacterial spore?

<p>Metabolically active (A)</p> Signup and view all the answers

What is the primary difference between bacteria and archaea?

<p>Archaea are found in extreme environments while bacteria are not. (D)</p> Signup and view all the answers

Which of these is an example of a non-cellular microorganism?

<p>Virus (A)</p> Signup and view all the answers

What is the name of the process by which bacteria form spores?

<p>Sporulation (D)</p> Signup and view all the answers

Which of the following is NOT a branch of microbiology?

<p>Botany (C)</p> Signup and view all the answers

What is the primary function of algae within the ecosystem?

<p>Nutrient production through photosynthesis (A)</p> Signup and view all the answers

Which type of microorganism is responsible for causing mad cow disease?

<p>Prion (D)</p> Signup and view all the answers

What is the primary difference between viroids and viruses?

<p>Viroids lack a protein coat, while viruses have a protein coat. (B)</p> Signup and view all the answers

What is the primary function of lysosomes in a cell?

<p>Breakdown of cellular waste and foreign particles (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of inflammation?

<p>Coldness (C)</p> Signup and view all the answers

What is the main function of cilia found on the surface of certain cells?

<p>Moving substances across the cell surface (C)</p> Signup and view all the answers

Which type of immune cell is responsible for producing antibodies?

<p>B-cells (B)</p> Signup and view all the answers

What is an example of a nonspecific defense mechanism?

<p>Phagocytosis by macrophages (D)</p> Signup and view all the answers

What is the function of interferon?

<p>Preventing viral infections in uninfected cells (D)</p> Signup and view all the answers

Which of the following is a characteristic of systemic inflammation?

<p>Increased white blood cell count (leukocytosis) (D)</p> Signup and view all the answers

What is the main difference between a killer T-cell and a memory T-cell?

<p>Killer T-cells destroy infected cells, while memory T-cells provide a faster and more intense immune response upon re-exposure to the same antigen (D)</p> Signup and view all the answers

What type of cell is responsible for the 'secondary response' in antibody-mediated immunity?

<p>Memory B-cells (D)</p> Signup and view all the answers

Which of the following accurately describes the process of germination in microorganisms?

<p>Dormant spore forms change into active vegetative forms (D)</p> Signup and view all the answers

Flashcards

Germination

Process where spore form changes into vegetative form when conditions improve.

Mitochondria

Organelles that produce ATP, the energy currency of the cell.

Ribosomes

Small RNA granules involved in protein synthesis, some are free, others attached to ER.

Endoplasmic Reticulum (ER)

Network for synthesis and transport; has smooth (lipids) & rough (proteins) types.

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Golgi Apparatus

Flat sacs that package and ship proteins and lipids from the ER.

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Lysosomes

Sacs filled with enzymes to digest waste and bacteria.

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Phagocytosis

Process by which cells ingest harmful particles; performed by phagocytes.

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Cell-mediated Immunity

Immunity produced by T-cells mainly against viruses and fungi.

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Antibody-mediated Immunity

Humoral immunity produced by B-cells mainly against bacteria.

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Normal Flora

Resident microorganisms that live in and on the body throughout a person's life.

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Transient Flora

Microorganisms that temporarily populate a host before disappearing.

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Opportunistic Pathogens

Normal flora that cause disease when the balance is disrupted.

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Contact Transmission

Transfer of pathogens through direct or indirect contact.

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Droplet Transmission

Spread of pathogens via respiratory droplets within 1 meter.

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Airborne Transmission

Pathogen spread through the air over distances greater than 1 meter.

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Food-borne Transmission

Spread of pathogens through contaminated food.

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Bodily Fluid Transmission

Spread of infections through human bodily fluids.

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Vector Transmission

Pathogen spread by animals, especially insects.

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Bioremediation

Using microorganisms to remove pollutants from the environment.

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Microbiology

The study of organisms too small to see without magnification.

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Microorganisms

Tiny organisms including bacteria, viruses, fungi, protozoa, and more.

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Prokaryotes

Organisms with no nucleus, e.g., bacteria and archaea.

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Eukaryotes

Organisms with a mature nucleus, e.g., fungi and algae.

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Taxonomy

The formal system of organizing and classifying living organisms.

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Sporulation

The process where vegetative cells form highly resistant spores.

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Endospores

Inert, resting forms of bacteria that can survive extreme conditions.

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Viruses

Noncellular entities that consist of nucleic acid and protein coat.

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Prions

Infectious proteins that can cause disease but lack nucleic acid.

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Study Notes

Microbiology

  • Study of organisms too small to see without magnification
  • Originated with invention of microscope
  • Microorganisms include bacteria, viruses, fungi, protozoa, helminths (worms), algae, prions

Branches of Study

  • Immunology
  • Public health microbiology and epidemiology
  • Food, dairy, and aquatic microbiology
  • Biotechnology
  • Genetic engineering and recombinant DNA technology
  • Manipulating genes

Microbes' Roles

  • Nutrient production and energy flow
  • Decomposition
  • Biotechnology (food, drug, vaccine production)
  • Genetic engineering
  • Bioremediation
  • Infectious disease

Classification of Microorganisms (Protists)

I - Cellular

  • Prokaryotes: lack a true nucleus, include archaea, bacteria, & blue-green algae
  • Eukaryotes: have a nucleus, examples include algae (except blue-green), fungi, & protozoans.

II - Non-Cellular

  • Viruses, viroids, prions

  • Taxonomy: The formal system of organizing, classifying, and naming living organisms. - Bacteria: prokaryotic, unicellular organisms that are found in varied environments - Archaea: prokaryotic, unicellular organisms that thrive in harsh environments (high salt, extreme temperatures) - Algae: eukaryotic, unicellular or multicellular organisms, that photosynthesize - Fungi: eukaryotic, unicellular or multicellular organisms - Protozoa: eukaryotic, unicellular or multicellular organisms that are animal-like - Viruses: noncellular particles, need electron microscopy for visualization

Bacterial Life Cycle

  • Vegetative Form: metabolically active, growing, and multiplying, causes infection
  • Spore Form: inert, resting, non-growing, and non-multiplying resistant form

Cytoplasmic Organelles

  • Mitochondria: powerhouses of cells, generate ATP
  • Ribosomes: protein synthesis
  • Endoplasmic Reticulum (ER): lipid and protein synthesis; transport - Smooth ER: lipid synthesis - Rough ER: protein synthesis
  • Golgi Apparatus: shipping center for cellular products
  • Lysosomes: digestive enzymes
  • Centrioles: involved in cell division
  • Cilia/Flagella: movement of substances/ cells
  • Chloroplasts: (in algae and plants) convert light energy into chemical energy

Defense Mechanism Against Infection

  • Nonspecific Mechanisms: act against all harmful agents (nonspecific resistance)
  • Specific Mechanisms: act against specific agents (immunity)
  • Virulence: degree of pathogenicity or disease-causing power

Nonspecific Defense Mechanisms

  • Intact skin and mucous membranes
  • Fluids (tears, saliva, urine) flush pathogens
  • Complements (proteins) activate phagocytosis and inflammation
  • Interferons: protect uninfected cells from viruses
  • Phagocytosis (neutrophils, monocytes/macrophages)
  • Inflammation (redness, heat, swelling, pain)

Specific Defense Mechanisms (Immunity)

  • Cell-mediated Immunity: uses T-cells (killer T-cells, memory T-cells) targeting viruses and fungi
  • Antibody-mediated Immunity: uses B-cells (plasma cells, memory B-cells) targeting bacteria with antibodies

Normal Flora (Microbiota)

  • Resident Flora: normal microbiota present throughout a person's life
  • Transient Flora: microorganisms that remain for a short time

Modes of Transmission

  • Contact (direct or indirect)
  • Droplet (less than 1 meter)
  • Airborne (greater than 1 meter)
  • Waterborne and Foodborne
  • Vectorborne.

Applied Microbiology

  • Includes various applications (food production, alcoholic beverages, water treatment, pharmaceuticals, agriculture, bioremediation, forensics, energy)

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