Microbiology Overview and Classification
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Questions and Answers

Which of the following is an example of a fomite?

  • A contaminated needle (correct)
  • A coughing person
  • An infected animal
  • A mosquito
  • Which of the following is a characteristic of airborne transmission?

  • The pathogen is transmitted through direct contact.
  • The pathogen travels less than a meter.
  • The pathogen is transmitted through contaminated food.
  • The pathogen travels more than 1 meter. (correct)
  • What is the term for bacteria that live in a healthy person without causing harm but can become pathogens under certain conditions?

  • Commensal bacteria
  • Transient flora
  • Virulent bacteria
  • Opportunistic pathogens (correct)
  • Which of the following is NOT a mode of transmission for infectious agents?

    <p>Genetic inheritance (B)</p> Signup and view all the answers

    Which of the following diseases is NOT typically transmitted through contaminated food or water?

    <p>Measles (A)</p> Signup and view all the answers

    What type of vector carries pathogens passively on their body parts?

    <p>Mechanical vector (A)</p> Signup and view all the answers

    Which of the following is an example of an application of applied microbiology in the medical field?

    <p>Development of antibiotics (D)</p> Signup and view all the answers

    Which of the following is an example of a transient flora?

    <p>Bacillus laterosporus (B)</p> Signup and view all the answers

    Which of the following is a major factor that can disrupt the balance of normal flora and lead to opportunistic infections?

    <p>Weakened immune system (B)</p> Signup and view all the answers

    What term describes the transmission of a pathogen through direct physical contact from one person to another?

    <p>Direct contact transmission (B)</p> Signup and view all the answers

    Which of these organisms are classified as prokaryotes?

    <p>Bacteria (C)</p> Signup and view all the answers

    Which branch of microbiology focuses on the study of how microorganisms impact human health?

    <p>Public health microbiology (B)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of a bacterial spore?

    <p>Metabolically active (A)</p> Signup and view all the answers

    What is the primary difference between bacteria and archaea?

    <p>Archaea are found in extreme environments while bacteria are not. (D)</p> Signup and view all the answers

    Which of these is an example of a non-cellular microorganism?

    <p>Virus (A)</p> Signup and view all the answers

    What is the name of the process by which bacteria form spores?

    <p>Sporulation (D)</p> Signup and view all the answers

    Which of the following is NOT a branch of microbiology?

    <p>Botany (C)</p> Signup and view all the answers

    What is the primary function of algae within the ecosystem?

    <p>Nutrient production through photosynthesis (A)</p> Signup and view all the answers

    Which type of microorganism is responsible for causing mad cow disease?

    <p>Prion (D)</p> Signup and view all the answers

    What is the primary difference between viroids and viruses?

    <p>Viroids lack a protein coat, while viruses have a protein coat. (B)</p> Signup and view all the answers

    What is the primary function of lysosomes in a cell?

    <p>Breakdown of cellular waste and foreign particles (D)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of inflammation?

    <p>Coldness (C)</p> Signup and view all the answers

    What is the main function of cilia found on the surface of certain cells?

    <p>Moving substances across the cell surface (C)</p> Signup and view all the answers

    Which type of immune cell is responsible for producing antibodies?

    <p>B-cells (B)</p> Signup and view all the answers

    What is an example of a nonspecific defense mechanism?

    <p>Phagocytosis by macrophages (D)</p> Signup and view all the answers

    What is the function of interferon?

    <p>Preventing viral infections in uninfected cells (D)</p> Signup and view all the answers

    Which of the following is a characteristic of systemic inflammation?

    <p>Increased white blood cell count (leukocytosis) (D)</p> Signup and view all the answers

    What is the main difference between a killer T-cell and a memory T-cell?

    <p>Killer T-cells destroy infected cells, while memory T-cells provide a faster and more intense immune response upon re-exposure to the same antigen (D)</p> Signup and view all the answers

    What type of cell is responsible for the 'secondary response' in antibody-mediated immunity?

    <p>Memory B-cells (D)</p> Signup and view all the answers

    Which of the following accurately describes the process of germination in microorganisms?

    <p>Dormant spore forms change into active vegetative forms (D)</p> Signup and view all the answers

    Flashcards

    Germination

    Process where spore form changes into vegetative form when conditions improve.

    Mitochondria

    Organelles that produce ATP, the energy currency of the cell.

    Ribosomes

    Small RNA granules involved in protein synthesis, some are free, others attached to ER.

    Endoplasmic Reticulum (ER)

    Network for synthesis and transport; has smooth (lipids) & rough (proteins) types.

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    Golgi Apparatus

    Flat sacs that package and ship proteins and lipids from the ER.

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    Lysosomes

    Sacs filled with enzymes to digest waste and bacteria.

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    Phagocytosis

    Process by which cells ingest harmful particles; performed by phagocytes.

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    Cell-mediated Immunity

    Immunity produced by T-cells mainly against viruses and fungi.

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    Antibody-mediated Immunity

    Humoral immunity produced by B-cells mainly against bacteria.

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    Normal Flora

    Resident microorganisms that live in and on the body throughout a person's life.

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    Transient Flora

    Microorganisms that temporarily populate a host before disappearing.

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    Opportunistic Pathogens

    Normal flora that cause disease when the balance is disrupted.

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    Contact Transmission

    Transfer of pathogens through direct or indirect contact.

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    Droplet Transmission

    Spread of pathogens via respiratory droplets within 1 meter.

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    Airborne Transmission

    Pathogen spread through the air over distances greater than 1 meter.

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    Food-borne Transmission

    Spread of pathogens through contaminated food.

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    Bodily Fluid Transmission

    Spread of infections through human bodily fluids.

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    Vector Transmission

    Pathogen spread by animals, especially insects.

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    Bioremediation

    Using microorganisms to remove pollutants from the environment.

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    Microbiology

    The study of organisms too small to see without magnification.

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    Microorganisms

    Tiny organisms including bacteria, viruses, fungi, protozoa, and more.

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    Prokaryotes

    Organisms with no nucleus, e.g., bacteria and archaea.

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    Eukaryotes

    Organisms with a mature nucleus, e.g., fungi and algae.

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    Taxonomy

    The formal system of organizing and classifying living organisms.

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    Sporulation

    The process where vegetative cells form highly resistant spores.

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    Endospores

    Inert, resting forms of bacteria that can survive extreme conditions.

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    Viruses

    Noncellular entities that consist of nucleic acid and protein coat.

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    Prions

    Infectious proteins that can cause disease but lack nucleic acid.

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    Study Notes

    Microbiology

    • Study of organisms too small to see without magnification
    • Originated with invention of microscope
    • Microorganisms include bacteria, viruses, fungi, protozoa, helminths (worms), algae, prions

    Branches of Study

    • Immunology
    • Public health microbiology and epidemiology
    • Food, dairy, and aquatic microbiology
    • Biotechnology
    • Genetic engineering and recombinant DNA technology
    • Manipulating genes

    Microbes' Roles

    • Nutrient production and energy flow
    • Decomposition
    • Biotechnology (food, drug, vaccine production)
    • Genetic engineering
    • Bioremediation
    • Infectious disease

    Classification of Microorganisms (Protists)

    I - Cellular

    • Prokaryotes: lack a true nucleus, include archaea, bacteria, & blue-green algae
    • Eukaryotes: have a nucleus, examples include algae (except blue-green), fungi, & protozoans.

    II - Non-Cellular

    • Viruses, viroids, prions

    • Taxonomy: The formal system of organizing, classifying, and naming living organisms. - Bacteria: prokaryotic, unicellular organisms that are found in varied environments - Archaea: prokaryotic, unicellular organisms that thrive in harsh environments (high salt, extreme temperatures) - Algae: eukaryotic, unicellular or multicellular organisms, that photosynthesize - Fungi: eukaryotic, unicellular or multicellular organisms - Protozoa: eukaryotic, unicellular or multicellular organisms that are animal-like - Viruses: noncellular particles, need electron microscopy for visualization

    Bacterial Life Cycle

    • Vegetative Form: metabolically active, growing, and multiplying, causes infection
    • Spore Form: inert, resting, non-growing, and non-multiplying resistant form

    Cytoplasmic Organelles

    • Mitochondria: powerhouses of cells, generate ATP
    • Ribosomes: protein synthesis
    • Endoplasmic Reticulum (ER): lipid and protein synthesis; transport - Smooth ER: lipid synthesis - Rough ER: protein synthesis
    • Golgi Apparatus: shipping center for cellular products
    • Lysosomes: digestive enzymes
    • Centrioles: involved in cell division
    • Cilia/Flagella: movement of substances/ cells
    • Chloroplasts: (in algae and plants) convert light energy into chemical energy

    Defense Mechanism Against Infection

    • Nonspecific Mechanisms: act against all harmful agents (nonspecific resistance)
    • Specific Mechanisms: act against specific agents (immunity)
    • Virulence: degree of pathogenicity or disease-causing power

    Nonspecific Defense Mechanisms

    • Intact skin and mucous membranes
    • Fluids (tears, saliva, urine) flush pathogens
    • Complements (proteins) activate phagocytosis and inflammation
    • Interferons: protect uninfected cells from viruses
    • Phagocytosis (neutrophils, monocytes/macrophages)
    • Inflammation (redness, heat, swelling, pain)

    Specific Defense Mechanisms (Immunity)

    • Cell-mediated Immunity: uses T-cells (killer T-cells, memory T-cells) targeting viruses and fungi
    • Antibody-mediated Immunity: uses B-cells (plasma cells, memory B-cells) targeting bacteria with antibodies

    Normal Flora (Microbiota)

    • Resident Flora: normal microbiota present throughout a person's life
    • Transient Flora: microorganisms that remain for a short time

    Modes of Transmission

    • Contact (direct or indirect)
    • Droplet (less than 1 meter)
    • Airborne (greater than 1 meter)
    • Waterborne and Foodborne
    • Vectorborne.

    Applied Microbiology

    • Includes various applications (food production, alcoholic beverages, water treatment, pharmaceuticals, agriculture, bioremediation, forensics, energy)

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