Microbiology of Vibrio and Related Bacteria
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Questions and Answers

What type of colonies do Vibrio species produce on TCBS agar?

  • Blue or green colonies
  • Yellow or green colonies (correct)
  • Clear colonies
  • Red or white colonies
  • Which organism does not require salt for growth?

  • Vibrio parahaemolyticus
  • Chromobacterium violaceum
  • Vibrio harveyi
  • Vibrio cholerae (correct)
  • What is the primary mode of transmission for cholera?

  • Fecal-oral route (correct)
  • Vector-borne transmission
  • Direct contact transmission
  • Airborne transmission
  • What is used for enrichment broth to obtain growth of vibrios from stool samples?

    <p>Alkaline peptone water (D)</p> Signup and view all the answers

    In addition to the fecal-oral route, what other method can lead to cholera infections?

    <p>Ingestion of contaminated water (B)</p> Signup and view all the answers

    Which method is directly used for observing pathogens under a microscope?

    <p>Gram stain (D)</p> Signup and view all the answers

    What is the primary purpose of using sheep blood agar in cultivation?

    <p>To exhibit hemolysis (A)</p> Signup and view all the answers

    What factor combination is essential for growing certain bacteria using culture media?

    <p>Both X and V factors (B)</p> Signup and view all the answers

    Which pathogen is known to be an uncommon cause of endocarditis?

    <p>H. aphrophilus (B)</p> Signup and view all the answers

    What is the recommended swabbing technique for collecting samples?

    <p>Swab in selective medium within 10 minutes (B)</p> Signup and view all the answers

    Which media provides both hemin and NAD necessary for certain bacterial growth?

    <p>Chocolate Agar (C)</p> Signup and view all the answers

    What is centrifugation generally used for in the detection methods?

    <p>Concentration of bacterial cells (D)</p> Signup and view all the answers

    The term 'satelliting' in blood agar refers to which phenomenon?

    <p>Dependency on V factor for growth (C)</p> Signup and view all the answers

    What is the primary habitat of Helicobacter pylori?

    <p>Human gastric mucosa (B)</p> Signup and view all the answers

    Which of the following is NOT a possible route of transmission for Helicobacter pylori?

    <p>Airborne droplets (B)</p> Signup and view all the answers

    What is one of the main modes of transmission for infections caused by Pasteurella multocida?

    <p>Animal bites or scratches (B)</p> Signup and view all the answers

    Which of the following best describes the role of the capsule in Pasteurella multocida?

    <p>It protects against immune attack (A)</p> Signup and view all the answers

    What structure is associated with the adhesion of Bordetella pertussis?

    <p>Fimbriae (C)</p> Signup and view all the answers

    Which organ system is NOT mentioned as being infected by Pasteurella multocida?

    <p>Nervous system (B)</p> Signup and view all the answers

    Which toxin produced by Bordetella pertussis is related to the cholera toxin?

    <p>Pertussis toxin (B)</p> Signup and view all the answers

    What complication can arise from bacteremia associated with Pasteurella multocida infections?

    <p>Metastatic abscess formation (D)</p> Signup and view all the answers

    What is the function of the Tracheal colonization factor in Bordetella pertussis?

    <p>Facilitate attachment to respiratory epithelium (B)</p> Signup and view all the answers

    What characterizes the habitat of Pasteurella multocida?

    <p>Normal flora of wild and domestic animals (D)</p> Signup and view all the answers

    Which of the following factors allows Helicobacter pylori to move within the stomach?

    <p>Flagella (A)</p> Signup and view all the answers

    Which procedure is specifically noted for its special consideration in the laboratory diagnosis of Pasteurella infections?

    <p>Gram stain analysis (D)</p> Signup and view all the answers

    What is the role of adenylate cyclase toxin produced by Bordetella pertussis?

    <p>Hemolyzes red cells and activates cyclic AMP (C)</p> Signup and view all the answers

    What is the significance of the dermonecrotic toxin in Bordetella pertussis?

    <p>Causes tissue damage (C)</p> Signup and view all the answers

    Which of the following is NOT a risk factor for Pasteurella multocida infections?

    <p>Contaminated food handling (A)</p> Signup and view all the answers

    What type of bacteria is Pasteurella multocida classified as?

    <p>Gram-negative bacilli (D)</p> Signup and view all the answers

    What method is commonly used for the direct detection of B. pertussis in smears from nasopharyngeal material?

    <p>One step immunochromatographic assay (A)</p> Signup and view all the answers

    Which of the following is not a method for identifying Bordetella pertussis?

    <p>Inoculation in nutrient broth (B)</p> Signup and view all the answers

    What type of vaccines are used for the prevention of pertussis?

    <p>Whole cell vaccines and acellular vaccines (A)</p> Signup and view all the answers

    Which organism is part of the Mycobacterium tuberculosis complex?

    <p>Mycobacterium bovis (A)</p> Signup and view all the answers

    Which characteristic do anaerobic bacteria possess?

    <p>They will not grow in the presence of oxygen (A)</p> Signup and view all the answers

    Which of the following is a type of Nontuberculous Mycobacterium?

    <p>All of the above (D)</p> Signup and view all the answers

    What is a key characteristic of organisms belonging to the genus Mycobacterium?

    <p>They cannot be cultured under aerobic conditions (C)</p> Signup and view all the answers

    What is the primary virulence factor associated with Bordetella pertussis?

    <p>Adenylate cyclase toxin (C)</p> Signup and view all the answers

    What type of organism is responsible for tetanus?

    <p>Clostridium tetani (D)</p> Signup and view all the answers

    What primarily causes botulism?

    <p>Ingestion of preformed toxin (A)</p> Signup and view all the answers

    Which of the following correctly describes the effects of tetanospasmin?

    <p>It causes generalized muscle spasms. (D)</p> Signup and view all the answers

    What is a common size range for aerosols produced in certain infections?

    <p>1 to 5 μm (C)</p> Signup and view all the answers

    What is a significant consequence of untreated muscle spasms caused by tetanospasmin?

    <p>Respiratory failure (A)</p> Signup and view all the answers

    What type of environment can contribute to botulism toxin absorption?

    <p>Nonacidic vegetable or mushroom foodstuffs (C)</p> Signup and view all the answers

    What can trigger spasms in individuals affected by tetanus?

    <p>A mild touch or minor stimuli (A)</p> Signup and view all the answers

    Which maneuver can also produce infectious aerosols in a laboratory?

    <p>Manipulations of lesions (C)</p> Signup and view all the answers

    Flashcards

    Vibrio cholerae

    A bacterium that can cause cholera, a serious diarrheal disease.

    TCBS Agar

    A type of culture medium that is selective for Vibrio species. It contains bile salts and thiosulfate, which inhibit the growth of other bacteria.

    Alkaline Peptone Water

    A specific type of water that is used to enrich the growth of Vibrio species. It is alkaline and has a pH of 8.4.

    Fecal-Oral Route

    A type of transmission in which the pathogen moves from the feces of an infected person to another individual through contaminated water or food.

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    Brackish or Salt Water

    An environment that is salty or has a high salt concentration. Certain bacteria thrive in this kind of environment.

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    Pasteurella multocida Infection

    A type of bacterial infection often acquired through animal bites or scratches.

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    Endotoxin

    A component of the outer membrane of certain bacteria, released upon bacterial lysis, and contributing to inflammation.

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    Antiphagocytic Capsule

    The capsule surrounding some bacteria prevents immune system cells from engulfing and destroying the bacteria.

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    Bacteremia

    An infection that spreads from the initial site to the bloodstream.

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    Metastatic Abscess Formation

    The spread of an infection from the original site to distant locations within the body.

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    Epidemiology

    The study of the occurrence, distribution, and determinants of health and disease in populations.

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    Mode of Transmission

    How a pathogen spreads from one person to another.

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    Normal Flora

    Bacteria found naturally in a particular environment.

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    Helicobacter pylori

    The bacterium responsible for causing gastritis, peptic ulcers, and stomach cancer. It is a curved, microaerophilic, Gram-negative rod and colonizes the stomach lining.

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    Where does H. pylori live?

    The primary habitat for H. pylori is within the human gastric mucosa.

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    Why is H. pylori's motility important?

    H. pylori's ability to move helps it navigate the stomach lining.

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    How does H. pylori affect the stomach lining?

    H. pylori doesn't invade the stomach tissue but sits on the surface.

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    How is H. pylori spread?

    The mode of transmission for H. pylori is still not fully understood. Current theories include oral-oral, fecal-oral, and mother-to-child spread.

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    How does H. pylori adhere to the stomach?

    H. pylori uses various factors and structures like fimbriae, FHA, pertactin, and BrkA, to stick to the stomach lining.

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    What is the role of Pertussis toxin in Bordetella pertussis?

    The Pertussis toxin produced by Bordetella pertussis is a potent toxin that can cause significant damage to the host.

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    What disease does Bordetella pertussis cause?

    Bordetella pertussis causes Whooping Cough, a serious respiratory illness.

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    Gram Stain

    A staining technique used to visualize bacteria under a microscope. It differentiates bacteria based on their cell wall structure.

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    CAP Agar

    A type of culture medium used to grow Haemophilus species in a lab setting. This medium provides essential nutrients like hemin (X factor) and NAD (V factor) that allow for the growth of these bacteria.

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    Sheep Blood Agar

    A type of culture medium that is often incorporated with sheep blood. It allows Haemophilus species to thrive, as the blood provides the V factor needed (NAD). This medium helps in observing a phenomenon known as "satelliting", where Haemophilus species grow in a ring around colonies of Staphylococcus species that are able to produce V factor.

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    Haemophilus ducreyi

    A specific type of Haemophilus species known for its ability to cause chancroid, a sexually transmitted infection.

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    Mueller Hinton based Chocolate Agar supplemented with 1% Isovitalex

    A type of medium used to grow Haemophilus ducreyi in a lab setting. It is a chocolate agar (which contains heated blood) that is supplemented with Isovitalex, a mixture of essential growth factors.

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    Antigen Detection

    A method of detecting Haemophilus influenzae type b (Hib) by identifying its capsular polysaccharide antigen, which is a specific molecule found on the surface of the bacteria.

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    Direct Observation

    A method of detecting Haemophilus infection by directly observing the bacteria under a microscope using a Gram stain.

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    Porphyrin Test

    A test used to determine the presence of specific enzymes in Haemophilus bacteria. It is a way to indirectly identify the bacteria based on the products produced by these specific enzymes.

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    CAP (Charcoal Agar with Potato)

    A type of bacterial culture medium that is specifically designed to grow Bordetella pertussis, the bacterium responsible for whooping cough.

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    Skirrow's Agar

    A bacterial culture medium used to grow and isolate anaerobic bacteria (those that cannot survive in oxygen).

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    Modified Thayer-Martin Agar

    A type of bacterial culture medium that is selective for the growth of Neisseria gonorrhoeae and Neisseria meningitidis. This agar typically includes a combination of antibiotics that inhibit the growth of most other bacteria.

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    Diagnostic Sensitivity

    The ability of a diagnostic test to correctly identify individuals who have the disease.

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    Columbia Agar

    A type of bacterial culture medium that is selective for the growth of Streptococcus pyogenes.

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    Cultivation

    A bacterial culture technique that involves culturing bacteria on a solid medium and then using a microscope to visualize the colonies of bacteria generated.

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    PCR (Polymerase Chain Reaction)

    A method to identify and analyze DNA fragments in a sample, often used for diagnosing infections.

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    Clostridium tetani

    A bacterium that causes tetanus, characterized by muscle spasms and potentially fatal respiratory failure.

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    Tetanospasmin

    A substance that disrupts nerve impulses, specifically causing muscle spasms in tetanus.

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    Aerosols

    Tiny particles of infectious material released into the air, often by coughing, sneezing, or speaking.

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    Reservoir

    The primary source from which an infectious agent can be transmitted to a susceptible host.

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    Wound infection

    The process by which an organism, like a bacterium, enters the body and establishes an infection.

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    Neurotoxin

    A toxin produced by certain bacteria that acts on the nervous system, causing paralysis.

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    Botulism

    The ingestion of a preformed toxin produced by bacteria, leading to food poisoning and paralysis.

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    Study Notes

    Vibrio, Plesiomonas, and Chromobacterium

    • These microorganisms are often found in contaminated water, food, and soil.
    • Vibrio cholerae: Transmission is fecal-oral route, contaminated water/food. Has two key toxins: Cholera toxin and Somatic O antigen. Key test is to distinguish from other halophilic Vibrio species using NaCl and string tests.
    • Vibrio parahaemolyticus: Transmitted through ingestion of contaminated raw or undercooked seafood.
    • Aeromonas spp.: Transmission via raw or undercooked contaminated seafood and water.
    • Plesiomonas shigelloides: Ingestion of contaminated seafood and/or water.
    • Chromobacterium violaceum: Exposure to contaminated soil or water.

    Pathogenesis and Spectrum of Disease

    • Vibrio cholerae: Causes diarrheal illness, characterized by rice-watery stools.
    • Types of infections depend on the specific species and can include gastroenteritis, wound infections, and bacteremia.
    • Laboratory Diagnosis: ELISA and latex agglutination tests are used to detect specific antigens or antibodies.
    • Other organisms: Have various modes of transmission and produce different types of toxins.

    Pasteurella and Similar Organisms

    • General characteristics: Small, gram-negative bacilli, nonmotile, oxidase positive, ferment glucose.
    • Habitat and Transmission: Associated with the nasopharynx and gastrointestinal tracts of wild and domestic animals. Transmission mainly by bites or scratches from animals.
    • Virulence factors: Some types have endotoxin (contributing to inflammation) and/or antiphagocytic capsules.
    • Laboratory Diagnosis: Special considerations are needed for proper specimen collection for testing.

    Haemophilus

    • Habitat and transmission: Found as part of the normal flora of the respiratory tract in humans. Transmission is likely via respiratory droplets.
    • Pathogenesis and Spectrum of Disease (H. influenzae): Causes various infections, such as meningitis, epiglottitis, and pneumonia in different biogroups.
    • Pathogenesis and Spectrum of Disease (H. ducreyi): Causes chancroid, a sexually transmitted disease (STD), characterized by painful genital ulcers.
    • Laboratory Diagnosis: Special considerations for specimens, due to the fastidious nature of the bacteria and the need for specific growth factors.

    Campylobacter, Arcobacter, and Helicobacter

    • Helicobacter pylori: Found in the human stomach. Mode of transmission is unknown; possible routes are oral-oral and fecal-oral.
    • Virulence factors: Includes CagA protein, adhesin, inflammation mediators, and cytotoxin.
    • Conditions caused: Gastritis and peptic ulcer disease.
    • Laboratory Diagnosis: Tissue biopsy is a useful diagnostic method.
    • Other organisms: Have different pathogenic mechanisms and clinical presentations.

    Anaerobic Bacteria

    • General Characteristics: Cannot grow in the presence of oxygen.
    • Specific examples: Clostridium perfringens, Clostridium tetani, Clostridium botulinum, and Clostridium difficile.
    • Associated diseases: Gas gangrene (C. perfringens), Tetanus (C. tetani), Botulism (C. botulinum), and antibiotic-associated diarrhea (C. difficile).

    Mycobacteria

    • General Characteristics: Slow-growing, aerobic bacteria.
    • Spectrum of Disease: Mycobacterium tuberculosis complex causes tuberculosis.
    • Mycobacteria tuberculosis complex: Infections occur via airborne droplet nuclei.
    • Other Mycobacteria: Have different diseases and transmission pathways.

    Spirochetes

    • General characteristics: Long, slender, helically curved, Gram-negative bacteria with axial fibrils.
    • Specific examples: Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans.
    • Spectrum of disease (Treponema pallidum): Syphilis
    • Other organisms: Have different disease conditions and transmission pathways.

    Obligate Intracellular Bacteria

    • Chlamydia trachomatis: Associated with several infections.
    • Laboratory Diagnosis: Uses special techniques to detect the organism.

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    Description

    Explore the fascinating world of Vibrio, Plesiomonas, and Chromobacterium. This quiz covers their transmission routes, diseases caused, and laboratory diagnosis methods. Test your knowledge on these important pathogens commonly associated with contaminated water and food.

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