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Questions and Answers
What type of colonies do Vibrio species produce on TCBS agar?
What type of colonies do Vibrio species produce on TCBS agar?
Which organism does not require salt for growth?
Which organism does not require salt for growth?
What is the primary mode of transmission for cholera?
What is the primary mode of transmission for cholera?
What is used for enrichment broth to obtain growth of vibrios from stool samples?
What is used for enrichment broth to obtain growth of vibrios from stool samples?
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In addition to the fecal-oral route, what other method can lead to cholera infections?
In addition to the fecal-oral route, what other method can lead to cholera infections?
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Which method is directly used for observing pathogens under a microscope?
Which method is directly used for observing pathogens under a microscope?
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What is the primary purpose of using sheep blood agar in cultivation?
What is the primary purpose of using sheep blood agar in cultivation?
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What factor combination is essential for growing certain bacteria using culture media?
What factor combination is essential for growing certain bacteria using culture media?
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Which pathogen is known to be an uncommon cause of endocarditis?
Which pathogen is known to be an uncommon cause of endocarditis?
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What is the recommended swabbing technique for collecting samples?
What is the recommended swabbing technique for collecting samples?
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Which media provides both hemin and NAD necessary for certain bacterial growth?
Which media provides both hemin and NAD necessary for certain bacterial growth?
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What is centrifugation generally used for in the detection methods?
What is centrifugation generally used for in the detection methods?
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The term 'satelliting' in blood agar refers to which phenomenon?
The term 'satelliting' in blood agar refers to which phenomenon?
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What is the primary habitat of Helicobacter pylori?
What is the primary habitat of Helicobacter pylori?
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Which of the following is NOT a possible route of transmission for Helicobacter pylori?
Which of the following is NOT a possible route of transmission for Helicobacter pylori?
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What is one of the main modes of transmission for infections caused by Pasteurella multocida?
What is one of the main modes of transmission for infections caused by Pasteurella multocida?
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Which of the following best describes the role of the capsule in Pasteurella multocida?
Which of the following best describes the role of the capsule in Pasteurella multocida?
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What structure is associated with the adhesion of Bordetella pertussis?
What structure is associated with the adhesion of Bordetella pertussis?
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Which organ system is NOT mentioned as being infected by Pasteurella multocida?
Which organ system is NOT mentioned as being infected by Pasteurella multocida?
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Which toxin produced by Bordetella pertussis is related to the cholera toxin?
Which toxin produced by Bordetella pertussis is related to the cholera toxin?
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What complication can arise from bacteremia associated with Pasteurella multocida infections?
What complication can arise from bacteremia associated with Pasteurella multocida infections?
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What is the function of the Tracheal colonization factor in Bordetella pertussis?
What is the function of the Tracheal colonization factor in Bordetella pertussis?
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What characterizes the habitat of Pasteurella multocida?
What characterizes the habitat of Pasteurella multocida?
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Which of the following factors allows Helicobacter pylori to move within the stomach?
Which of the following factors allows Helicobacter pylori to move within the stomach?
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Which procedure is specifically noted for its special consideration in the laboratory diagnosis of Pasteurella infections?
Which procedure is specifically noted for its special consideration in the laboratory diagnosis of Pasteurella infections?
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What is the role of adenylate cyclase toxin produced by Bordetella pertussis?
What is the role of adenylate cyclase toxin produced by Bordetella pertussis?
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What is the significance of the dermonecrotic toxin in Bordetella pertussis?
What is the significance of the dermonecrotic toxin in Bordetella pertussis?
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Which of the following is NOT a risk factor for Pasteurella multocida infections?
Which of the following is NOT a risk factor for Pasteurella multocida infections?
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What type of bacteria is Pasteurella multocida classified as?
What type of bacteria is Pasteurella multocida classified as?
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What method is commonly used for the direct detection of B. pertussis in smears from nasopharyngeal material?
What method is commonly used for the direct detection of B. pertussis in smears from nasopharyngeal material?
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Which of the following is not a method for identifying Bordetella pertussis?
Which of the following is not a method for identifying Bordetella pertussis?
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What type of vaccines are used for the prevention of pertussis?
What type of vaccines are used for the prevention of pertussis?
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Which organism is part of the Mycobacterium tuberculosis complex?
Which organism is part of the Mycobacterium tuberculosis complex?
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Which characteristic do anaerobic bacteria possess?
Which characteristic do anaerobic bacteria possess?
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Which of the following is a type of Nontuberculous Mycobacterium?
Which of the following is a type of Nontuberculous Mycobacterium?
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What is a key characteristic of organisms belonging to the genus Mycobacterium?
What is a key characteristic of organisms belonging to the genus Mycobacterium?
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What is the primary virulence factor associated with Bordetella pertussis?
What is the primary virulence factor associated with Bordetella pertussis?
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What type of organism is responsible for tetanus?
What type of organism is responsible for tetanus?
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What primarily causes botulism?
What primarily causes botulism?
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Which of the following correctly describes the effects of tetanospasmin?
Which of the following correctly describes the effects of tetanospasmin?
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What is a common size range for aerosols produced in certain infections?
What is a common size range for aerosols produced in certain infections?
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What is a significant consequence of untreated muscle spasms caused by tetanospasmin?
What is a significant consequence of untreated muscle spasms caused by tetanospasmin?
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What type of environment can contribute to botulism toxin absorption?
What type of environment can contribute to botulism toxin absorption?
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What can trigger spasms in individuals affected by tetanus?
What can trigger spasms in individuals affected by tetanus?
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Which maneuver can also produce infectious aerosols in a laboratory?
Which maneuver can also produce infectious aerosols in a laboratory?
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Flashcards
Vibrio cholerae
Vibrio cholerae
A bacterium that can cause cholera, a serious diarrheal disease.
TCBS Agar
TCBS Agar
A type of culture medium that is selective for Vibrio species. It contains bile salts and thiosulfate, which inhibit the growth of other bacteria.
Alkaline Peptone Water
Alkaline Peptone Water
A specific type of water that is used to enrich the growth of Vibrio species. It is alkaline and has a pH of 8.4.
Fecal-Oral Route
Fecal-Oral Route
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Brackish or Salt Water
Brackish or Salt Water
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Pasteurella multocida Infection
Pasteurella multocida Infection
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Endotoxin
Endotoxin
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Antiphagocytic Capsule
Antiphagocytic Capsule
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Bacteremia
Bacteremia
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Metastatic Abscess Formation
Metastatic Abscess Formation
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Epidemiology
Epidemiology
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Mode of Transmission
Mode of Transmission
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Normal Flora
Normal Flora
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Helicobacter pylori
Helicobacter pylori
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Where does H. pylori live?
Where does H. pylori live?
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Why is H. pylori's motility important?
Why is H. pylori's motility important?
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How does H. pylori affect the stomach lining?
How does H. pylori affect the stomach lining?
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How is H. pylori spread?
How is H. pylori spread?
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How does H. pylori adhere to the stomach?
How does H. pylori adhere to the stomach?
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What is the role of Pertussis toxin in Bordetella pertussis?
What is the role of Pertussis toxin in Bordetella pertussis?
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What disease does Bordetella pertussis cause?
What disease does Bordetella pertussis cause?
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Gram Stain
Gram Stain
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CAP Agar
CAP Agar
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Sheep Blood Agar
Sheep Blood Agar
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Haemophilus ducreyi
Haemophilus ducreyi
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Mueller Hinton based Chocolate Agar supplemented with 1% Isovitalex
Mueller Hinton based Chocolate Agar supplemented with 1% Isovitalex
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Antigen Detection
Antigen Detection
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Direct Observation
Direct Observation
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Porphyrin Test
Porphyrin Test
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CAP (Charcoal Agar with Potato)
CAP (Charcoal Agar with Potato)
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Skirrow's Agar
Skirrow's Agar
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Modified Thayer-Martin Agar
Modified Thayer-Martin Agar
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Diagnostic Sensitivity
Diagnostic Sensitivity
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Columbia Agar
Columbia Agar
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Cultivation
Cultivation
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PCR (Polymerase Chain Reaction)
PCR (Polymerase Chain Reaction)
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Clostridium tetani
Clostridium tetani
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Tetanospasmin
Tetanospasmin
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Aerosols
Aerosols
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Reservoir
Reservoir
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Wound infection
Wound infection
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Neurotoxin
Neurotoxin
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Botulism
Botulism
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Study Notes
Vibrio, Plesiomonas, and Chromobacterium
- These microorganisms are often found in contaminated water, food, and soil.
- Vibrio cholerae: Transmission is fecal-oral route, contaminated water/food. Has two key toxins: Cholera toxin and Somatic O antigen. Key test is to distinguish from other halophilic Vibrio species using NaCl and string tests.
- Vibrio parahaemolyticus: Transmitted through ingestion of contaminated raw or undercooked seafood.
- Aeromonas spp.: Transmission via raw or undercooked contaminated seafood and water.
- Plesiomonas shigelloides: Ingestion of contaminated seafood and/or water.
- Chromobacterium violaceum: Exposure to contaminated soil or water.
Pathogenesis and Spectrum of Disease
- Vibrio cholerae: Causes diarrheal illness, characterized by rice-watery stools.
- Types of infections depend on the specific species and can include gastroenteritis, wound infections, and bacteremia.
- Laboratory Diagnosis: ELISA and latex agglutination tests are used to detect specific antigens or antibodies.
- Other organisms: Have various modes of transmission and produce different types of toxins.
Pasteurella and Similar Organisms
- General characteristics: Small, gram-negative bacilli, nonmotile, oxidase positive, ferment glucose.
- Habitat and Transmission: Associated with the nasopharynx and gastrointestinal tracts of wild and domestic animals. Transmission mainly by bites or scratches from animals.
- Virulence factors: Some types have endotoxin (contributing to inflammation) and/or antiphagocytic capsules.
- Laboratory Diagnosis: Special considerations are needed for proper specimen collection for testing.
Haemophilus
- Habitat and transmission: Found as part of the normal flora of the respiratory tract in humans. Transmission is likely via respiratory droplets.
- Pathogenesis and Spectrum of Disease (H. influenzae): Causes various infections, such as meningitis, epiglottitis, and pneumonia in different biogroups.
- Pathogenesis and Spectrum of Disease (H. ducreyi): Causes chancroid, a sexually transmitted disease (STD), characterized by painful genital ulcers.
- Laboratory Diagnosis: Special considerations for specimens, due to the fastidious nature of the bacteria and the need for specific growth factors.
Campylobacter, Arcobacter, and Helicobacter
- Helicobacter pylori: Found in the human stomach. Mode of transmission is unknown; possible routes are oral-oral and fecal-oral.
- Virulence factors: Includes CagA protein, adhesin, inflammation mediators, and cytotoxin.
- Conditions caused: Gastritis and peptic ulcer disease.
- Laboratory Diagnosis: Tissue biopsy is a useful diagnostic method.
- Other organisms: Have different pathogenic mechanisms and clinical presentations.
Anaerobic Bacteria
- General Characteristics: Cannot grow in the presence of oxygen.
- Specific examples: Clostridium perfringens, Clostridium tetani, Clostridium botulinum, and Clostridium difficile.
- Associated diseases: Gas gangrene (C. perfringens), Tetanus (C. tetani), Botulism (C. botulinum), and antibiotic-associated diarrhea (C. difficile).
Mycobacteria
- General Characteristics: Slow-growing, aerobic bacteria.
- Spectrum of Disease: Mycobacterium tuberculosis complex causes tuberculosis.
- Mycobacteria tuberculosis complex: Infections occur via airborne droplet nuclei.
- Other Mycobacteria: Have different diseases and transmission pathways.
Spirochetes
- General characteristics: Long, slender, helically curved, Gram-negative bacteria with axial fibrils.
- Specific examples: Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans.
- Spectrum of disease (Treponema pallidum): Syphilis
- Other organisms: Have different disease conditions and transmission pathways.
Obligate Intracellular Bacteria
- Chlamydia trachomatis: Associated with several infections.
- Laboratory Diagnosis: Uses special techniques to detect the organism.
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Description
Explore the fascinating world of Vibrio, Plesiomonas, and Chromobacterium. This quiz covers their transmission routes, diseases caused, and laboratory diagnosis methods. Test your knowledge on these important pathogens commonly associated with contaminated water and food.