Microbiology of Vibrio and Related Bacteria

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Questions and Answers

What type of colonies do Vibrio species produce on TCBS agar?

  • Blue or green colonies
  • Yellow or green colonies (correct)
  • Clear colonies
  • Red or white colonies

Which organism does not require salt for growth?

  • Vibrio parahaemolyticus
  • Chromobacterium violaceum
  • Vibrio harveyi
  • Vibrio cholerae (correct)

What is the primary mode of transmission for cholera?

  • Fecal-oral route (correct)
  • Vector-borne transmission
  • Direct contact transmission
  • Airborne transmission

What is used for enrichment broth to obtain growth of vibrios from stool samples?

<p>Alkaline peptone water (D)</p> Signup and view all the answers

In addition to the fecal-oral route, what other method can lead to cholera infections?

<p>Ingestion of contaminated water (B)</p> Signup and view all the answers

Which method is directly used for observing pathogens under a microscope?

<p>Gram stain (D)</p> Signup and view all the answers

What is the primary purpose of using sheep blood agar in cultivation?

<p>To exhibit hemolysis (A)</p> Signup and view all the answers

What factor combination is essential for growing certain bacteria using culture media?

<p>Both X and V factors (B)</p> Signup and view all the answers

Which pathogen is known to be an uncommon cause of endocarditis?

<p>H. aphrophilus (B)</p> Signup and view all the answers

What is the recommended swabbing technique for collecting samples?

<p>Swab in selective medium within 10 minutes (B)</p> Signup and view all the answers

Which media provides both hemin and NAD necessary for certain bacterial growth?

<p>Chocolate Agar (C)</p> Signup and view all the answers

What is centrifugation generally used for in the detection methods?

<p>Concentration of bacterial cells (D)</p> Signup and view all the answers

The term 'satelliting' in blood agar refers to which phenomenon?

<p>Dependency on V factor for growth (C)</p> Signup and view all the answers

What is the primary habitat of Helicobacter pylori?

<p>Human gastric mucosa (B)</p> Signup and view all the answers

Which of the following is NOT a possible route of transmission for Helicobacter pylori?

<p>Airborne droplets (B)</p> Signup and view all the answers

What is one of the main modes of transmission for infections caused by Pasteurella multocida?

<p>Animal bites or scratches (B)</p> Signup and view all the answers

Which of the following best describes the role of the capsule in Pasteurella multocida?

<p>It protects against immune attack (A)</p> Signup and view all the answers

What structure is associated with the adhesion of Bordetella pertussis?

<p>Fimbriae (C)</p> Signup and view all the answers

Which organ system is NOT mentioned as being infected by Pasteurella multocida?

<p>Nervous system (B)</p> Signup and view all the answers

Which toxin produced by Bordetella pertussis is related to the cholera toxin?

<p>Pertussis toxin (B)</p> Signup and view all the answers

What complication can arise from bacteremia associated with Pasteurella multocida infections?

<p>Metastatic abscess formation (D)</p> Signup and view all the answers

What is the function of the Tracheal colonization factor in Bordetella pertussis?

<p>Facilitate attachment to respiratory epithelium (B)</p> Signup and view all the answers

What characterizes the habitat of Pasteurella multocida?

<p>Normal flora of wild and domestic animals (D)</p> Signup and view all the answers

Which of the following factors allows Helicobacter pylori to move within the stomach?

<p>Flagella (A)</p> Signup and view all the answers

Which procedure is specifically noted for its special consideration in the laboratory diagnosis of Pasteurella infections?

<p>Gram stain analysis (D)</p> Signup and view all the answers

What is the role of adenylate cyclase toxin produced by Bordetella pertussis?

<p>Hemolyzes red cells and activates cyclic AMP (C)</p> Signup and view all the answers

What is the significance of the dermonecrotic toxin in Bordetella pertussis?

<p>Causes tissue damage (C)</p> Signup and view all the answers

Which of the following is NOT a risk factor for Pasteurella multocida infections?

<p>Contaminated food handling (A)</p> Signup and view all the answers

What type of bacteria is Pasteurella multocida classified as?

<p>Gram-negative bacilli (D)</p> Signup and view all the answers

What method is commonly used for the direct detection of B. pertussis in smears from nasopharyngeal material?

<p>One step immunochromatographic assay (A)</p> Signup and view all the answers

Which of the following is not a method for identifying Bordetella pertussis?

<p>Inoculation in nutrient broth (B)</p> Signup and view all the answers

What type of vaccines are used for the prevention of pertussis?

<p>Whole cell vaccines and acellular vaccines (A)</p> Signup and view all the answers

Which organism is part of the Mycobacterium tuberculosis complex?

<p>Mycobacterium bovis (A)</p> Signup and view all the answers

Which characteristic do anaerobic bacteria possess?

<p>They will not grow in the presence of oxygen (A)</p> Signup and view all the answers

Which of the following is a type of Nontuberculous Mycobacterium?

<p>All of the above (D)</p> Signup and view all the answers

What is a key characteristic of organisms belonging to the genus Mycobacterium?

<p>They cannot be cultured under aerobic conditions (C)</p> Signup and view all the answers

What is the primary virulence factor associated with Bordetella pertussis?

<p>Adenylate cyclase toxin (C)</p> Signup and view all the answers

What type of organism is responsible for tetanus?

<p>Clostridium tetani (D)</p> Signup and view all the answers

What primarily causes botulism?

<p>Ingestion of preformed toxin (A)</p> Signup and view all the answers

Which of the following correctly describes the effects of tetanospasmin?

<p>It causes generalized muscle spasms. (D)</p> Signup and view all the answers

What is a common size range for aerosols produced in certain infections?

<p>1 to 5 μm (C)</p> Signup and view all the answers

What is a significant consequence of untreated muscle spasms caused by tetanospasmin?

<p>Respiratory failure (A)</p> Signup and view all the answers

What type of environment can contribute to botulism toxin absorption?

<p>Nonacidic vegetable or mushroom foodstuffs (C)</p> Signup and view all the answers

What can trigger spasms in individuals affected by tetanus?

<p>A mild touch or minor stimuli (A)</p> Signup and view all the answers

Which maneuver can also produce infectious aerosols in a laboratory?

<p>Manipulations of lesions (C)</p> Signup and view all the answers

Flashcards

Vibrio cholerae

A bacterium that can cause cholera, a serious diarrheal disease.

TCBS Agar

A type of culture medium that is selective for Vibrio species. It contains bile salts and thiosulfate, which inhibit the growth of other bacteria.

Alkaline Peptone Water

A specific type of water that is used to enrich the growth of Vibrio species. It is alkaline and has a pH of 8.4.

Fecal-Oral Route

A type of transmission in which the pathogen moves from the feces of an infected person to another individual through contaminated water or food.

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Brackish or Salt Water

An environment that is salty or has a high salt concentration. Certain bacteria thrive in this kind of environment.

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Pasteurella multocida Infection

A type of bacterial infection often acquired through animal bites or scratches.

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Endotoxin

A component of the outer membrane of certain bacteria, released upon bacterial lysis, and contributing to inflammation.

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Antiphagocytic Capsule

The capsule surrounding some bacteria prevents immune system cells from engulfing and destroying the bacteria.

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Bacteremia

An infection that spreads from the initial site to the bloodstream.

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Metastatic Abscess Formation

The spread of an infection from the original site to distant locations within the body.

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Epidemiology

The study of the occurrence, distribution, and determinants of health and disease in populations.

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Mode of Transmission

How a pathogen spreads from one person to another.

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Normal Flora

Bacteria found naturally in a particular environment.

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Helicobacter pylori

The bacterium responsible for causing gastritis, peptic ulcers, and stomach cancer. It is a curved, microaerophilic, Gram-negative rod and colonizes the stomach lining.

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Where does H. pylori live?

The primary habitat for H. pylori is within the human gastric mucosa.

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Why is H. pylori's motility important?

H. pylori's ability to move helps it navigate the stomach lining.

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How does H. pylori affect the stomach lining?

H. pylori doesn't invade the stomach tissue but sits on the surface.

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How is H. pylori spread?

The mode of transmission for H. pylori is still not fully understood. Current theories include oral-oral, fecal-oral, and mother-to-child spread.

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How does H. pylori adhere to the stomach?

H. pylori uses various factors and structures like fimbriae, FHA, pertactin, and BrkA, to stick to the stomach lining.

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What is the role of Pertussis toxin in Bordetella pertussis?

The Pertussis toxin produced by Bordetella pertussis is a potent toxin that can cause significant damage to the host.

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What disease does Bordetella pertussis cause?

Bordetella pertussis causes Whooping Cough, a serious respiratory illness.

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Gram Stain

A staining technique used to visualize bacteria under a microscope. It differentiates bacteria based on their cell wall structure.

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CAP Agar

A type of culture medium used to grow Haemophilus species in a lab setting. This medium provides essential nutrients like hemin (X factor) and NAD (V factor) that allow for the growth of these bacteria.

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Sheep Blood Agar

A type of culture medium that is often incorporated with sheep blood. It allows Haemophilus species to thrive, as the blood provides the V factor needed (NAD). This medium helps in observing a phenomenon known as "satelliting", where Haemophilus species grow in a ring around colonies of Staphylococcus species that are able to produce V factor.

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Haemophilus ducreyi

A specific type of Haemophilus species known for its ability to cause chancroid, a sexually transmitted infection.

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Mueller Hinton based Chocolate Agar supplemented with 1% Isovitalex

A type of medium used to grow Haemophilus ducreyi in a lab setting. It is a chocolate agar (which contains heated blood) that is supplemented with Isovitalex, a mixture of essential growth factors.

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Antigen Detection

A method of detecting Haemophilus influenzae type b (Hib) by identifying its capsular polysaccharide antigen, which is a specific molecule found on the surface of the bacteria.

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Direct Observation

A method of detecting Haemophilus infection by directly observing the bacteria under a microscope using a Gram stain.

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Porphyrin Test

A test used to determine the presence of specific enzymes in Haemophilus bacteria. It is a way to indirectly identify the bacteria based on the products produced by these specific enzymes.

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CAP (Charcoal Agar with Potato)

A type of bacterial culture medium that is specifically designed to grow Bordetella pertussis, the bacterium responsible for whooping cough.

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Skirrow's Agar

A bacterial culture medium used to grow and isolate anaerobic bacteria (those that cannot survive in oxygen).

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Modified Thayer-Martin Agar

A type of bacterial culture medium that is selective for the growth of Neisseria gonorrhoeae and Neisseria meningitidis. This agar typically includes a combination of antibiotics that inhibit the growth of most other bacteria.

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Diagnostic Sensitivity

The ability of a diagnostic test to correctly identify individuals who have the disease.

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Columbia Agar

A type of bacterial culture medium that is selective for the growth of Streptococcus pyogenes.

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Cultivation

A bacterial culture technique that involves culturing bacteria on a solid medium and then using a microscope to visualize the colonies of bacteria generated.

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PCR (Polymerase Chain Reaction)

A method to identify and analyze DNA fragments in a sample, often used for diagnosing infections.

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Clostridium tetani

A bacterium that causes tetanus, characterized by muscle spasms and potentially fatal respiratory failure.

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Tetanospasmin

A substance that disrupts nerve impulses, specifically causing muscle spasms in tetanus.

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Aerosols

Tiny particles of infectious material released into the air, often by coughing, sneezing, or speaking.

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Reservoir

The primary source from which an infectious agent can be transmitted to a susceptible host.

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Wound infection

The process by which an organism, like a bacterium, enters the body and establishes an infection.

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Neurotoxin

A toxin produced by certain bacteria that acts on the nervous system, causing paralysis.

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Botulism

The ingestion of a preformed toxin produced by bacteria, leading to food poisoning and paralysis.

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Study Notes

Vibrio, Plesiomonas, and Chromobacterium

  • These microorganisms are often found in contaminated water, food, and soil.
  • Vibrio cholerae: Transmission is fecal-oral route, contaminated water/food. Has two key toxins: Cholera toxin and Somatic O antigen. Key test is to distinguish from other halophilic Vibrio species using NaCl and string tests.
  • Vibrio parahaemolyticus: Transmitted through ingestion of contaminated raw or undercooked seafood.
  • Aeromonas spp.: Transmission via raw or undercooked contaminated seafood and water.
  • Plesiomonas shigelloides: Ingestion of contaminated seafood and/or water.
  • Chromobacterium violaceum: Exposure to contaminated soil or water.

Pathogenesis and Spectrum of Disease

  • Vibrio cholerae: Causes diarrheal illness, characterized by rice-watery stools.
  • Types of infections depend on the specific species and can include gastroenteritis, wound infections, and bacteremia.
  • Laboratory Diagnosis: ELISA and latex agglutination tests are used to detect specific antigens or antibodies.
  • Other organisms: Have various modes of transmission and produce different types of toxins.

Pasteurella and Similar Organisms

  • General characteristics: Small, gram-negative bacilli, nonmotile, oxidase positive, ferment glucose.
  • Habitat and Transmission: Associated with the nasopharynx and gastrointestinal tracts of wild and domestic animals. Transmission mainly by bites or scratches from animals.
  • Virulence factors: Some types have endotoxin (contributing to inflammation) and/or antiphagocytic capsules.
  • Laboratory Diagnosis: Special considerations are needed for proper specimen collection for testing.

Haemophilus

  • Habitat and transmission: Found as part of the normal flora of the respiratory tract in humans. Transmission is likely via respiratory droplets.
  • Pathogenesis and Spectrum of Disease (H. influenzae): Causes various infections, such as meningitis, epiglottitis, and pneumonia in different biogroups.
  • Pathogenesis and Spectrum of Disease (H. ducreyi): Causes chancroid, a sexually transmitted disease (STD), characterized by painful genital ulcers.
  • Laboratory Diagnosis: Special considerations for specimens, due to the fastidious nature of the bacteria and the need for specific growth factors.

Campylobacter, Arcobacter, and Helicobacter

  • Helicobacter pylori: Found in the human stomach. Mode of transmission is unknown; possible routes are oral-oral and fecal-oral.
  • Virulence factors: Includes CagA protein, adhesin, inflammation mediators, and cytotoxin.
  • Conditions caused: Gastritis and peptic ulcer disease.
  • Laboratory Diagnosis: Tissue biopsy is a useful diagnostic method.
  • Other organisms: Have different pathogenic mechanisms and clinical presentations.

Anaerobic Bacteria

  • General Characteristics: Cannot grow in the presence of oxygen.
  • Specific examples: Clostridium perfringens, Clostridium tetani, Clostridium botulinum, and Clostridium difficile.
  • Associated diseases: Gas gangrene (C. perfringens), Tetanus (C. tetani), Botulism (C. botulinum), and antibiotic-associated diarrhea (C. difficile).

Mycobacteria

  • General Characteristics: Slow-growing, aerobic bacteria.
  • Spectrum of Disease: Mycobacterium tuberculosis complex causes tuberculosis.
  • Mycobacteria tuberculosis complex: Infections occur via airborne droplet nuclei.
  • Other Mycobacteria: Have different diseases and transmission pathways.

Spirochetes

  • General characteristics: Long, slender, helically curved, Gram-negative bacteria with axial fibrils.
  • Specific examples: Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans.
  • Spectrum of disease (Treponema pallidum): Syphilis
  • Other organisms: Have different disease conditions and transmission pathways.

Obligate Intracellular Bacteria

  • Chlamydia trachomatis: Associated with several infections.
  • Laboratory Diagnosis: Uses special techniques to detect the organism.

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