Microbiology Media Terms Quiz

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Questions and Answers

What is the purpose of streak plate method on agar plate?

To obtain isolated, individual colonies (correct)
To observe bacterial motility
To create a mixed culture
To promote the growth of microorganisms

Which type of media consists of broth and agar?

Differential media
Selective media
Nutrient media
Solid media (correct)

What does the term 'inoculate' refer to in microbiology?

The process of preparing media components
The process of observing bacterial colonies
The process of sterilizing media
The process of adding microorganisms to media (correct)

Which method is used for inoculating agar slant?

Fish Tail method (C) Signup and view all the answers

What is the purpose of using differential media?

To separate bacteria based on a metabolic process (A) Signup and view all the answers

Which agar is used to isolate and differentiate gram-negative organisms?

EMB agar (D) Signup and view all the answers

What is the purpose of using selective media?

To encourage the growth of some bacteria while discouraging others (D) Signup and view all the answers

What is the indicator for mannitol fermentation in MSA (Mannitol Salt Agar)?

Phenol red (B) Signup and view all the answers

What is the purpose of using MacConkey agar?

To isolate and differentiate gram-negative organisms (C) Signup and view all the answers

What does the presence of black color in SIM tubes indicate?

Hydrogen sulfide (H2S) production (C) Signup and view all the answers

What is the purpose of using blood agar?

Isolation and cultivation of fastidious bacteria (B) Signup and view all the answers

What is the function of the Durham tube in Phenol Red Broths?

To trap gas produced during carbohydrate fermentation (A) Signup and view all the answers

What is the purpose of using Starch Agar?

To check for the presence of amylase via starch hydrolysis (D) Signup and view all the answers

What is the purpose of using Eosin Methylene Blue Agar (EMB)?

To isolate Gram-negative bacteria (C) Signup and view all the answers

What is the purpose of using MSA (Mannitol Salt Agar)?

For isolation and differentiation of Staphylococcus species (A) Signup and view all the answers

What does the colorless appearance of colonies on MacConkey agar indicate?

No lactose fermentation (B) Signup and view all the answers

Match the type of media with its description:

Broth = Liquid media Solid = Broth + Agar media Agar Plate = Used for streak to isolate/Quadrant Method Agar Slant = Used for Fish Tail method Signup and view all the answers

Match the method of inoculating media with the appropriate media type:

Streak to isolate/Quadrant Method = Agar Plate Dip – n – Swish Method = Broth Fish Tail method = Agar Slant Straight Line Stab Streak To Isolate Method = Agar Deep Signup and view all the answers

Match the term with its definition:

Media = Material used for the growth of microorganisms Inoculate = The process by which microorganisms are added to media Colony = A group of genetically identical bacteria Selective and Differential Media Components = Media components with specific functions Signup and view all the answers

Match the agar plate method with its purpose:

Streak to isolate/Quadrant Method = To obtain isolated, individual colonies Dip – n – Swish Method = N/A Fish Tail method = N/A Straight Line Stab Streak To Isolate Method = N/A Signup and view all the answers

Match the bacterial colonies on agar with the appropriate method:

Bacterium 1 - 12 = Streak To Isolate Method N/A = N/A Signup and view all the answers

Match the following agar media with their primary purpose:

Blood agar = Isolation and cultivation of fastidious bacteria MacConkey Agar = Isolate and differentiate gram negative organisms Starch Agar = To check for the presence of amylase via starch hydrolysis EMB (Eosin Methylene Blue Agar) = Isolate Gram negative bacteria Signup and view all the answers

Match the following methods with their primary usage:

Dip-n-Swish Method = Used to inoculate broth with an inoculating loop Fish Tail Method = Used to inoculate an agar slant with an inoculating loop Straight Line Stab = Used to inoculate an agar deep with an inoculating needle Hot Pink Colonies on MacConkey Agar = (+) for lactose fermentation Signup and view all the answers

Match the following terms with their corresponding definition:

Differential media = Helps separate bacteria based on a metabolic process Selective media = Contains an ingredient that encourages the growth of some bacteria, but discourages the growth of other bacteria Beta-hemolysis = Complete breakdown of hemoglobin; clearing of medium Transport media = Used for transporting specimens to the laboratory Signup and view all the answers

Match the following tubes with their test results:

Phenol Red Broths = Used to determine carbohydrate fermentation with or without gas production SIM tubes = Sulfide, indole, motility; used to determine H2S production, indole presence, and motility Carbohydrate Utilization Tubes = Used for enzyme tests to determine carbohydrate fermentation MSA (Mannitol Salt Agar) = For isolation and differentiation of Staphylococcus species Signup and view all the answers

Match the following agar media with their indicator and corresponding color result:

MSA (Mannitol Salt Agar) = Indicator: phenol red; Yellow = acidic = (+) for mannitol fermentation EMB (Eosin Methylene Blue Agar) = Indicator: neutral red; Metallic green/blue black colonies = (+) vigorous lactose fermentors Phenol Red Broths = Indicator: phenol red; A for acid (yellow) = (+) for carbohydrate fermentation Starch Agar = Reagent: Iodine; Halo = (+) for Amylase Signup and view all the answers

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Study Notes

Microbiology Media and Techniques

Streak plate method is used to isolate and identify individual bacterial colonies on an agar plate.

Media Types

Semi-solid media consists of broth and agar.
Differential media is used to distinguish between different types of bacteria based on their growth characteristics.
Selective media is used to selectively grow certain types of bacteria while inhibiting others.
Mannitol Salt Agar (MSA) is used to isolate and differentiate gram-positive organisms.
MacConkey agar is used to isolate and differentiate gram-negative organisms.

Inoculation Techniques

Inoculate refers to the introduction of microorganisms into a medium or culture.
The agar slant is inoculated using the slope method.

Media Purpose and Indicators

Blood agar is used to detect hemolytic activity of bacteria.
The Durham tube in Phenol Red Broths is used to detect gas production.
Starch Agar is used to detect starch hydrolysis.
Eosin Methylene Blue Agar (EMB) is used to differentiate between lactose-fermenting and non-lactose-fermenting bacteria.
MSA (Mannitol Salt Agar) is used to detect mannitol fermentation, indicated by a yellow color.

Test Results

The presence of black color in SIM tubes indicates the production of hydrogen sulfide.
Colorless appearance of colonies on MacConkey agar indicates non-lactose fermentation.

Media and Method Matches

Agar slant: slope method
Starch Agar: detect starch hydrolysis
Blood agar: detect hemolytic activity
MacConkey agar: isolate and differentiate gram-negative organisms
Durham tube: detect gas production
Eosin Methylene Blue Agar (EMB): differentiate between lactose-fermenting and non-lactose-fermenting bacteria
MSA (Mannitol Salt Agar): detect mannitol fermentation
SIM tubes: detect hydrogen sulfide production