Microbiology Media Terms Quiz

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Questions and Answers

What is the purpose of streak plate method on agar plate?

To obtain isolated, individual colonies (correct)
To observe bacterial motility
To create a mixed culture
To promote the growth of microorganisms

Which type of media consists of broth and agar?

Differential media
Selective media
Nutrient media
Solid media (correct)

What does the term 'inoculate' refer to in microbiology?

The process of preparing media components
The process of observing bacterial colonies
The process of sterilizing media
The process of adding microorganisms to media (correct)

Which method is used for inoculating agar slant?

Fish Tail method (C)

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What is the purpose of using differential media?

To separate bacteria based on a metabolic process (A)

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Which agar is used to isolate and differentiate gram-negative organisms?

EMB agar (D)

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What is the purpose of using selective media?

To encourage the growth of some bacteria while discouraging others (D)

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What is the indicator for mannitol fermentation in MSA (Mannitol Salt Agar)?

Phenol red (B)

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What is the purpose of using MacConkey agar?

To isolate and differentiate gram-negative organisms (C)

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What does the presence of black color in SIM tubes indicate?

Hydrogen sulfide (H2S) production (C)

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What is the purpose of using blood agar?

Isolation and cultivation of fastidious bacteria (B)

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What is the function of the Durham tube in Phenol Red Broths?

To trap gas produced during carbohydrate fermentation (A)

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What is the purpose of using Starch Agar?

To check for the presence of amylase via starch hydrolysis (D)

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What is the purpose of using Eosin Methylene Blue Agar (EMB)?

To isolate Gram-negative bacteria (C)

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What is the purpose of using MSA (Mannitol Salt Agar)?

For isolation and differentiation of Staphylococcus species (A)

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What does the colorless appearance of colonies on MacConkey agar indicate?

No lactose fermentation (B)

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Match the type of media with its description:

Broth = Liquid media Solid = Broth + Agar media Agar Plate = Used for streak to isolate/Quadrant Method Agar Slant = Used for Fish Tail method

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Match the method of inoculating media with the appropriate media type:

Streak to isolate/Quadrant Method = Agar Plate Dip – n – Swish Method = Broth Fish Tail method = Agar Slant Straight Line Stab Streak To Isolate Method = Agar Deep

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Match the term with its definition:

Media = Material used for the growth of microorganisms Inoculate = The process by which microorganisms are added to media Colony = A group of genetically identical bacteria Selective and Differential Media Components = Media components with specific functions

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Match the agar plate method with its purpose:

Streak to isolate/Quadrant Method = To obtain isolated, individual colonies Dip – n – Swish Method = N/A Fish Tail method = N/A Straight Line Stab Streak To Isolate Method = N/A

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Match the bacterial colonies on agar with the appropriate method:

Bacterium 1 - 12 = Streak To Isolate Method N/A = N/A

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Match the following agar media with their primary purpose:

Blood agar = Isolation and cultivation of fastidious bacteria MacConkey Agar = Isolate and differentiate gram negative organisms Starch Agar = To check for the presence of amylase via starch hydrolysis EMB (Eosin Methylene Blue Agar) = Isolate Gram negative bacteria

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Match the following methods with their primary usage:

Dip-n-Swish Method = Used to inoculate broth with an inoculating loop Fish Tail Method = Used to inoculate an agar slant with an inoculating loop Straight Line Stab = Used to inoculate an agar deep with an inoculating needle Hot Pink Colonies on MacConkey Agar = (+) for lactose fermentation

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Match the following terms with their corresponding definition:

Differential media = Helps separate bacteria based on a metabolic process Selective media = Contains an ingredient that encourages the growth of some bacteria, but discourages the growth of other bacteria Beta-hemolysis = Complete breakdown of hemoglobin; clearing of medium Transport media = Used for transporting specimens to the laboratory

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Match the following tubes with their test results:

Phenol Red Broths = Used to determine carbohydrate fermentation with or without gas production SIM tubes = Sulfide, indole, motility; used to determine H2S production, indole presence, and motility Carbohydrate Utilization Tubes = Used for enzyme tests to determine carbohydrate fermentation MSA (Mannitol Salt Agar) = For isolation and differentiation of Staphylococcus species

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Match the following agar media with their indicator and corresponding color result:

MSA (Mannitol Salt Agar) = Indicator: phenol red; Yellow = acidic = (+) for mannitol fermentation EMB (Eosin Methylene Blue Agar) = Indicator: neutral red; Metallic green/blue black colonies = (+) vigorous lactose fermentors Phenol Red Broths = Indicator: phenol red; A for acid (yellow) = (+) for carbohydrate fermentation Starch Agar = Reagent: Iodine; Halo = (+) for Amylase

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Flashcards

Streak Plate Method Purpose

To obtain isolated, individual colonies of microorganisms.

Solid Media Composition

A type of media that contains both broth (liquid) and agar (solidifying agent).

Inoculate Definition

The process of introducing microorganisms into a culture medium.

Fish Tail Method

A method of inoculation used for agar slants, creating a zig-zag pattern.

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Purpose of Differential Media

To distinguish between different types of bacteria based on their metabolic activities.

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EMB Agar Purpose

A selective and differential agar used to isolate and differentiate Gram-negative organisms.

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Purpose of Selective Media

To promote the growth of certain bacteria while inhibiting the growth of others.

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MSA Indicator

Phenol red indicates whether mannitol fermentation has occurred in MSA. It turns yellow in acidic conditions, indicating fermentation.

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MacConkey Agar Purpose

To isolate and differentiate Gram-negative organisms.

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Black Color in SIM Tubes

Indicates the production of hydrogen sulfide (H2S) by the microorganism.

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Purpose of Blood Agar

For isolation and cultivation of bacteria that require specific growth factors (fastidious bacteria).

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Durham Tube Function

To trap any gas produced during the fermentation of carbohydrates.

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Purpose of Starch Agar

To determine if a microorganism produces amylase, an enzyme that hydrolyzes starch.

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EMB Agar Purpose

To selectively isolate Gram-negative bacteria.

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MSA Purpose

For the isolation and differentiation of Staphylococcus species, especially Staphylococcus aureus.

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Colorless Colonies on MacConkey

Indicates that the bacteria are unable to ferment lactose.

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Study Notes

Microbiology Media and Techniques

Streak plate method is used to isolate and identify individual bacterial colonies on an agar plate.

Media Types

Semi-solid media consists of broth and agar.
Differential media is used to distinguish between different types of bacteria based on their growth characteristics.
Selective media is used to selectively grow certain types of bacteria while inhibiting others.
Mannitol Salt Agar (MSA) is used to isolate and differentiate gram-positive organisms.
MacConkey agar is used to isolate and differentiate gram-negative organisms.

Inoculation Techniques

Inoculate refers to the introduction of microorganisms into a medium or culture.
The agar slant is inoculated using the slope method.

Media Purpose and Indicators

Blood agar is used to detect hemolytic activity of bacteria.
The Durham tube in Phenol Red Broths is used to detect gas production.
Starch Agar is used to detect starch hydrolysis.
Eosin Methylene Blue Agar (EMB) is used to differentiate between lactose-fermenting and non-lactose-fermenting bacteria.
MSA (Mannitol Salt Agar) is used to detect mannitol fermentation, indicated by a yellow color.

Test Results

The presence of black color in SIM tubes indicates the production of hydrogen sulfide.
Colorless appearance of colonies on MacConkey agar indicates non-lactose fermentation.

Media and Method Matches

Agar slant: slope method
Starch Agar: detect starch hydrolysis
Blood agar: detect hemolytic activity
MacConkey agar: isolate and differentiate gram-negative organisms
Durham tube: detect gas production
Eosin Methylene Blue Agar (EMB): differentiate between lactose-fermenting and non-lactose-fermenting bacteria
MSA (Mannitol Salt Agar): detect mannitol fermentation
SIM tubes: detect hydrogen sulfide production

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Microbiology Media Terms Quiz

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Questions and Answers

What is the purpose of streak plate method on agar plate?

Which type of media consists of broth and agar?

What does the term 'inoculate' refer to in microbiology?

Which method is used for inoculating agar slant?

What is the purpose of using differential media?

Which agar is used to isolate and differentiate gram-negative organisms?

What is the purpose of using selective media?

What is the indicator for mannitol fermentation in MSA (Mannitol Salt Agar)?

What is the purpose of using MacConkey agar?

What does the presence of black color in SIM tubes indicate?

What is the purpose of using blood agar?

What is the function of the Durham tube in Phenol Red Broths?

What is the purpose of using Starch Agar?

What is the purpose of using Eosin Methylene Blue Agar (EMB)?

What is the purpose of using MSA (Mannitol Salt Agar)?

What does the colorless appearance of colonies on MacConkey agar indicate?

Match the type of media with its description:

Match the method of inoculating media with the appropriate media type:

Match the term with its definition:

Match the agar plate method with its purpose:

Match the bacterial colonies on agar with the appropriate method:

Match the following agar media with their primary purpose:

Match the following methods with their primary usage:

Match the following terms with their corresponding definition:

Match the following tubes with their test results:

Match the following agar media with their indicator and corresponding color result:

Flashcards

Streak Plate Method Purpose

Solid Media Composition

Inoculate Definition

Fish Tail Method

Purpose of Differential Media

EMB Agar Purpose

Purpose of Selective Media

MSA Indicator

MacConkey Agar Purpose

Black Color in SIM Tubes

Purpose of Blood Agar

Durham Tube Function

Purpose of Starch Agar

EMB Agar Purpose

MSA Purpose

Colorless Colonies on MacConkey

Study Notes

Microbiology Media and Techniques

Media Types

Inoculation Techniques

Media Purpose and Indicators

Test Results

Media and Method Matches

Studying That Suits You

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