Microbiology Media Composition Quiz

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Questions and Answers

What is the characteristic of a chemically defined medium?

  • It contains a mixture of proteins and extracts
  • Exact amount of pure chemicals is known (correct)
  • Supports the growth of a wide variety of microbes
  • It provides minimal and basic growth requirements

Which type of media inhibits the growth of some bacteria while selecting for the growth of others?

  • Media composed of a mixture of proteins and extracts
  • Selective media (correct)
  • Complex nutrient media
  • General purpose media

What is the primary difference between general purpose media and selective media?

  • Selective media supports the growth of a wide variety of microbes
  • Selective media inhibits the growth of some bacteria while selecting for the growth of others (correct)
  • General purpose media contains known amounts of pure chemicals
  • General purpose media inhibits the growth of some bacteria

Which type of media provides the minimal and basic growth requirements for bacteria but not all microbes can grow on it?

<p>Chemically defined medium (C)</p> Signup and view all the answers

What is the composition of a complex medium?

<p>It contains a mixture of proteins and extracts (C)</p> Signup and view all the answers

What makes Mannitol Salt Agar a selective medium?

<p>It selects for salt tolerant bacteria (B)</p> Signup and view all the answers

What is the role of phenol red in Mannitol Salt Agar?

<p>It serves as a pH indicator (A)</p> Signup and view all the answers

Why is MacConkey Agar considered a selective medium?

<p>It contains bile salts and crystal violet that inhibit the growth of Gram-positive bacteria (D)</p> Signup and view all the answers

What is the function of neutral red in MacConkey Agar?

<p>It acts as a pH indicator (B)</p> Signup and view all the answers

What is the differential ingredient in MacConkey Agar?

<p>Lactose sugar (A)</p> Signup and view all the answers

Flashcards

Chemically Defined Medium

A medium where the exact amount of each pure chemical is known.

Selective Media

This type of media inhibits the growth of some bacteria while selecting for the growth of others.

Difference Between General and Selective Media

General purpose media supports a wide range of microbes, while selective media inhibits some and favors others.

Chemically Defined Medium

Provides the minimal, basic growth requirements, but not all microbes can grow on it.

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Complex Medium

Contains a mixture of proteins and extracts; exact composition is unknown.

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Mannitol Salt Agar's Selectivity

The high salt concentration selects for salt-tolerant bacteria.

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Phenol Red in Mannitol Salt Agar

It is a pH indicator that shows if mannitol fermentation has occurred, turning yellow in acidic conditions.

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MacConkey Agar's Selectivity

Bile salts and crystal violet inhibit Gram-positive bacteria, allowing Gram-negative bacteria to grow.

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Neutral Red in MacConkey Agar

It is a pH indicator that turns red in acidic conditions, indicating lactose fermentation.

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Differential Ingredient in MacConkey Agar

Lactose is fermented by some bacteria, leading to acid production and a color change.

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