Podcast
Questions and Answers
What is the primary reason for using immersion oil with the oil immersion lens?
What is the primary reason for using immersion oil with the oil immersion lens?
- To increase the numerical aperture and improve resolution. (correct)
- To improve contrast by reducing scattering of light.
- To increase the magnification of the objective lens.
- To prevent damage to the objective lens by reducing friction.
Which of the following actions is NOT considered a proper aseptic technique?
Which of the following actions is NOT considered a proper aseptic technique?
- Cooling the inoculating loop before introducing it into the culture.
- Holding a test tube at a 45° angle when transferring culture.
- Leaving the lid of a petri dish open while transferring culture. (correct)
- Flaming the inoculating loop before and after use.
Which of the following characteristics is NOT typically observed in bacterial growth in a broth culture?
Which of the following characteristics is NOT typically observed in bacterial growth in a broth culture?
- Turbidity (cloudiness)
- Uniform distribution of bacteria throughout the broth (correct)
- Surface film formation
- Pellet formation at the bottom
When performing a 4-quadrant streak plate, what is the primary goal?
When performing a 4-quadrant streak plate, what is the primary goal?
Which of the following is NOT a potential source of contamination to microbial cultures?
Which of the following is NOT a potential source of contamination to microbial cultures?
What is the term that describes the ability to distinguish between two closely spaced objects as separate entities under a microscope?
What is the term that describes the ability to distinguish between two closely spaced objects as separate entities under a microscope?
Which of the following actions is NOT critical for proper handling of a Bunsen burner?
Which of the following actions is NOT critical for proper handling of a Bunsen burner?
What is the primary function of a wet mount in microscopy?
What is the primary function of a wet mount in microscopy?
What type of staining technique is used to identify the presence of Trichomonas in a wet mount?
What type of staining technique is used to identify the presence of Trichomonas in a wet mount?
Which of the following bacterial shapes is NOT named after its scientific name?
Which of the following bacterial shapes is NOT named after its scientific name?
What is the main purpose of heat-fixing a bacterial smear before staining?
What is the main purpose of heat-fixing a bacterial smear before staining?
Which of the following is NOT a component of a Gram stain?
Which of the following is NOT a component of a Gram stain?
What is the primary reason for performing a Gram stain?
What is the primary reason for performing a Gram stain?
Flashcards
Wet Mount
Wet Mount
A lab technique used to examine fresh samples for specific infections like Trichomonas.
Bacterial Shapes
Bacterial Shapes
The three basic bacterial shapes are cocci (round), bacilli (rod), and spirilli (spiral).
Heat-Fixed Smear
Heat-Fixed Smear
A preparation technique to keep specimen fixed on a slide during staining.
Gram Staining
Gram Staining
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Mordant
Mordant
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Lab Safety Techniques
Lab Safety Techniques
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Bunsen Burner Usage
Bunsen Burner Usage
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Aseptic Technique
Aseptic Technique
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Microscope Parts
Microscope Parts
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Total Magnification
Total Magnification
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4-Quadrant Streak Plate
4-Quadrant Streak Plate
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Clinical Specimen Transport
Clinical Specimen Transport
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Study Notes
Lab Exam 1 Study Outline
- Lab Safety and Techniques: Review Lab Safety quiz (pages 1-4). Focus on proper Bunsen burner use, flame recognition, inoculating loop technique, tube capping, handling materials, and inoculation procedures for broths, slants, and plates.
Microscopy - Lab 1
- Microscope Parts: Learn the parts of the microscope and their functions.
- Microscopy Terms: Understand parfocal, resolution, and the reason oil immersion lenses use oil (same refractive index as glass to avoid light loss due to air).
- Magnification: Calculate total magnification.
Aseptic Technique - Labs 2-7
- Transfer of Cultures: Understand how to transfer cultures from slants, broths, or plate colonies.
- Inoculation: Learn how to inoculate slants, broths, and plates.
- Slide Preparation: Practice making wet mounts and heat-fixed smears.
- Contamination Control: Know possible sources of contamination (e.g., leaving cultures open, improper angle of holding).
Growth - Labs 2 and 7
- Growth Characteristics: Understand bacterial growth characteristics in broth.
- Streak Plate Technique: Master the 4-quadrant streak plate method to isolate mixed cultures.
- Pure Colonies: Recognize the goal of obtaining pure colonies using the streak plate technique.
Clinical Specimens
- Collection and Transport: Understand parameters for collecting/transporting clinical specimens. Refer back to the review quiz on page 2-12.
- Specimen Microscopy (Labs 3 & 4): Identify various specimens under a microscope, such as Trichomonas, Ova and Parasites.
- Bacterial Shapes Identification Learn to recognize and name bacterial shapes: cocci, bacilli, and spirilla. Use their scientific terms.
Staining - Simple, Lab 4
- Simple Stains: Understand how a simple stain, like methylene blue, works by knowing the charge of the stain.
Staining - Gram, Lab 5
- Gram Staining Procedure: Learn the Gram staining process, focusing on the roles of crystal violet, iodine, decolorizer, and safranin in differentiating Gram-positive and Gram-negative bacteria.
- Mordant role: Identify the role of iodine in the Gram-stain process.
- Practical Application: The reason why Gram staining is useful, i.e., identifying bacteria in less than 5 minutes.
- Culture Age: Understand why young cultures are important for reliable staining results.
Staining - Endospore, Lab 6
- Endospore Staining Procedure: Learn the technique for identifying endospore-forming bacteria.
- Heat Role: Use of heat fixing is necessary to drive the malachite green stain into the endospore.
- Importance of Bacterial Culture Age: Recognize why older cultures are necessary.
- Endospore Recognition: Can differentiate between endospores and vegetative bacterial cells.
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