Microbiology Lab Techniques Quiz
24 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary reason for emphasizing aseptic techniques in a microbiology lab?

To prevent contamination and disease spread from pathogenic microorganisms.

Explain the significance of understanding host and parasite relationships in microbiology.

It helps in understanding how diseases are caused and the mechanisms of infection.

Why is it necessary to use personal protective equipment (PPE) in a microbiology laboratory?

To protect individuals from exposure to harmful microorganisms and prevent laboratory-acquired infections.

What action should be taken if an equipment breakage occurs in the lab?

<p>Report the breakage immediately to the instructor.</p> Signup and view all the answers

Describe the protocol that must be followed before and after using an inoculating loop.

<p>Flame the inoculating loop or needle before and after each use to sterilize it.</p> Signup and view all the answers

What is the best prevention method to mitigate communicable diseases according to the course?

<p>Prevention is the best way rather than cure.</p> Signup and view all the answers

Why is it important to keep the workbench free from clusters of objects?

<p>To maintain a sterile workspace and reduce the risk of contamination during experiments.</p> Signup and view all the answers

What is the first step you must take before starting any laboratory exercise?

<p>Wash off the bench top with the disinfectant provided.</p> Signup and view all the answers

What is the primary purpose of a microscope in a laboratory setting?

<p>To observe tiny organisms that cannot be seen by the naked eye.</p> Signup and view all the answers

How does a freezer differ from a refrigerator in terms of material preservation?

<p>A freezer preserves materials below the freezing point of water.</p> Signup and view all the answers

Why is a hot air oven effective for sterilization?

<p>It operates effectively at temperatures between 160-300°C.</p> Signup and view all the answers

What is the function of biosafety cabinets in the laboratory?

<p>To prevent infections from very infectious bacteria.</p> Signup and view all the answers

What is the use of a pH meter in laboratory procedures?

<p>To measure the pH of media and reagents.</p> Signup and view all the answers

Describe the role of Durham tubes in fermentation media.

<p>They are used to detect gas production during sugar fermentation.</p> Signup and view all the answers

What is the purpose of an inoculation loop?

<p>To inoculate test samples into culture media.</p> Signup and view all the answers

How are microtitre plates utilized in laboratory testing?

<p>They are primarily used for ELISA tests.</p> Signup and view all the answers

Why is it essential to tie back long hair and wear fitted clothing in a microbiology laboratory?

<p>It prevents contamination of cultures and minimizes the risk of hair or loose clothing coming into contact with hazardous materials.</p> Signup and view all the answers

What is the purpose of sterilizing inoculating needles and loops before and after use?

<p>Sterilizing prevents the transfer of pathogenic microorganisms and maintains a sterile environment during procedures.</p> Signup and view all the answers

Describe the correct method for handling bottle stoppers in a microbiology lab.

<p>Stoppers should be held without touching the part that may become contaminated, and never placed on the bench.</p> Signup and view all the answers

What is the recommended procedure for flaming the loop before using it?

<p>The loop should be flamed until it is red hot, then allowed to cool before touching a culture.</p> Signup and view all the answers

Explain why the mouth of the tube must be flamed after inserting the inoculating loop.

<p>Flaming the mouth of the tube kills any potential contaminants that may have entered during the loop insertion.</p> Signup and view all the answers

What are two key pieces of equipment used for sterilization and culturing in a microbiology lab?

<p>An autoclave for sterilization and an incubator for culturing bacterial and fungal samples.</p> Signup and view all the answers

At what temperature and pressure does an autoclave operate effectively for sterilization?

<p>It operates at 121°C and 15 Psig for 15 minutes.</p> Signup and view all the answers

Why should lab personnel work with both hands during inoculation procedures?

<p>Using both hands allows one to hold the culture container while the other handles the inoculation loop, reducing the risk of contamination.</p> Signup and view all the answers

Flashcards

Aseptic Procedures

Methods to prevent contamination in microbiology.

Inoculating Tools

Needles and loops used for transferring cultures.

Flame Sterilization

Using fire to sterilize inoculating tools and containers.

Culture Handling

Procedures for safely managing bacterial and fungal cultures.

Signup and view all the flashcards

Autoclave

Equipment that sterilizes using high temperature and pressure.

Signup and view all the flashcards

Incubator

Device used for growing bacteria and fungi at controlled temperatures.

Signup and view all the flashcards

Microbial Culture

Process of growing microorganisms in a lab setting.

Signup and view all the flashcards

Personal Hygiene in Lab

Washing hands and tying back hair to avoid contamination.

Signup and view all the flashcards

Aseptic technique

Practices to prevent contamination by pathogens in microbiology.

Signup and view all the flashcards

Disinfection

Process to eliminate or reduce harmful microorganisms on surfaces or objects.

Signup and view all the flashcards

Sterilization

Complete destruction of all microorganisms in a material or object.

Signup and view all the flashcards

Pathogenic organisms

Microorganisms capable of causing disease.

Signup and view all the flashcards

Epidemiology

Study of how diseases spread and can be controlled in populations.

Signup and view all the flashcards

Immunity

The body's ability to resist or fight infection.

Signup and view all the flashcards

Personal protective equipment (PPE)

Gear that protects laboratory workers from exposure to hazards.

Signup and view all the flashcards

Laboratory rules

Guidelines ensuring safety and cleanliness in the lab environment.

Signup and view all the flashcards

Microscope

An instrument for observing tiny organisms not visible to the naked eye.

Signup and view all the flashcards

Refrigerator

Appliance used for preservation of samples, cultures, media, and sensitive materials at cool temperatures.

Signup and view all the flashcards

Freezer

Similar to a refrigerator, but preserves materials at temperatures below water's freezing point.

Signup and view all the flashcards

Hot Air Oven

Device used for sterilizing materials that cannot be exposed to moist heat, effective at 160-300°C.

Signup and view all the flashcards

Biosafety Cabinets

Safety equipment designed to prevent infections from infectious bacteria during lab work.

Signup and view all the flashcards

Vortex

A device used to mix liquids, especially in test tubes.

Signup and view all the flashcards

PH Meter

Instrument for measuring the pH of media and reagents.

Signup and view all the flashcards

Centrifuge

Equipment that separates components by spinning them at high speeds.

Signup and view all the flashcards

Study Notes

Introduction to Practical Course in Microbiology and Immunology

  • This course introduces practical applications of microbiology and immunology.
  • Students will learn basic microbiology knowledge, aseptic techniques, disinfection, and sterilization.
  • Handling materials contaminated with pathogenic organisms is a key component of the course.
  • The course aims to teach the biological characteristics of important disease-causing bacteria.
  • The relationship between microorganisms and diseases will be emphasized.
  • Students and staff must understand how microorganisms can cause disease and practice aseptic technique.
  • Students should be knowledgeable of epidemiology, pathogenesis, the host-parasite relationship and the development of resistance to infection.
  • The importance of prevention over cure is also highlighted.

General Laboratory Rules

  • Eating, drinking, and smoking in the lab are prohibited, including mouth pipetting.
  • Personal protective equipment (PPE) like lab coats and gloves is mandatory.
  • Barefoot entry is not allowed; shoes must be worn.
  • Report any equipment breakage to instructors promptly.
  • Keep the work area clear of unnecessary equipment.
  • Disinfect work surfaces before and after use.
  • Wash hands with soap and water before leaving the lab and whenever there's potential contamination.
  • Cultures cannot be removed from the lab without instructor permission.

Aseptic Procedures in Microbiology Laboratory

  • Procedures in microbiology labs should minimize the risk of contamination to oneself, the work area and cultures.
  • Inoculating needles and loops must be sterilized in a flame before and after use.
  • Bottles and tubes should not be touched unnecessarily.
  • Never put the lids/tops on benches.
  • Always flame the mouths of bottles and tubes before and after use.

Aseptic Technique in Microbiology

  • Flame loops in Bunsen burners/spirit lamps until red hot before and after use.
  • Introduce loops slowly to prevent spraying of materials.
  • Cool the loop down before picking cultures or specimens.
  • Hold the culture plate or tube while flaming the mouth, using correct hygiene procedures.
  • Replace lids/tops after use.
  • Sterilize tools thoroughly after use.
  • Everything and every surface in the lab is potentially infectious; exercise caution.

Important Equipment in Microbiology Laboratory

  • Microbiology is a broad scientific discipline involving techniques from microscopy to genetic analysis and much more.
  • The wide variety of techniques and testing require varied and specialized equipment.
  • Subsequent slides list the equipment and reagents for microbiological examination.

Autoclave

  • Equipment used for sterilizing glassware and media.
  • Effective sterilization occurs at 121°C and 15 Psig pressure for 15 minutes.

Incubator

  • Used for bacterial and fungal cultures (prokaryotic and eukaryotic cells).

Microscope

  • Observe tiny organisms that are not visible to the naked eye.
  • Aids in magnification and resolution.

Refrigerator

  • For sample, culture, media, and sensitive materials preservation.

Freezer

  • For sample, culture, media, and sensitive materials preservation at temperatures below freezing point of water

Hot Air Oven

  • Used for sterilizing materials not suitable for moist heat.
  • Effective sterilization occurs at 160-300°C.

Biosafety Cabinets

  • Prevention of infection, especially with highly infectious bacteria like Bacillus anthracis, various mycobacterium species, and aspergillus species.

Petri dishes/culture plates/agar plates

  • Support culture medium, contain cultures and specimens.
  • Sterile disposable swabs, e.g., for specimen collection;
  • Serological test slides (use with immunoassays).
  • Universal bottles are used for sample collection and media preparation.

Vortex

  • Mixing liquids in test tubes.

Magnetic Stirrer

  • Mixing liquids in beakers and conical flasks.

pH Meter

  • Measure pH of media and reagents.

Candle Jar

  • For cultivating anaerobic organisms.

Centrifuge

  • Separate supernatant and pellet materials.
  • Used for preserving cell samples, viruses, and fungal spores.

Durham tubes

  • Detect gas production in fermentation media.
  • Used for specific microbiological tests as part of overall lab testing procedures.

Haematologic (blood) Plates

  • Test for viral cultures.


Inoculation Loop

  • Transfer bacterial or fungal cultures.


Microtitre Plates

  • Used in ELISA tests.


Canister

  • Carrying and storage of petri dish equipment.


Washing Bottles

  • Used to wash equipment, commonly using distilled water.


Cool Box

  • Transport samples.


Vacutainer tubes

  • Blood sample collection.


Measuring Cylinder

  • Measure specific volumes of reagents/water.


Tally Counter

  • Count bacteria/colonies.


Micropipette

  • Measured volumes.


Water Bath

  • Warms culture media
  • Cannot be autoclaved
  • Used for maintaining a consistent temperature during certain procedures.

Weighing Balance

  • Measuring mass of media, chemicals, and reagents.

Syringes

  • For blood specimen collection.


Bijou Bottles

  • Hold culture medium and solutions for biochemical tests.

Safety Cabinet

safety cabinet is used as a critical piece of equipment in microbiology laboratories to handle potentially infectious materials safely.

Incubator(Details)

  • Copper steel chambers facilitate the circulation of warm water/air by electrical current, providing optimal growth conditions for microorganisms in the lab.


Electronic Balance

  • Weighs chemical substances accurately in lab settings.


Petri Plate(Details)

  • Developed by J.R. Petri.
  • Used for culturing various types of microorganisms in a controlled environment and observed for certain characteristics expected from the organism under study. 


Spirit Lamp


  • Heat chemical substances (e.g., within apparatus)


Bunsen Burner

  • Sterilizes metallic instruments, glass tubes, and flasks, heating components for important procedures.

Centrifuge (Details)

  • Separates sediments (typically biological or chemical) from a suspension.


Autoclave (Details)

  • Maintains high pressure and temperatures to effectively sterilize materials during lab procedures.
  • Sterilizes glassware, media, etc., for various microbiology lab procedures.

Inoculation Loop (Details)


  • Transferring microbiological growth, facilitating lab procedures.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Related Documents

Practical 1 Microbiology PDF

Description

Test your knowledge on critical practices in microbiology labs, including aseptic techniques, use of PPE, and equipment protocols. This quiz covers essential laboratory procedures that ensure safety and accuracy in microbiological research. Understanding these concepts is vital for anyone working in the field.

More Like This

Microbiology_5_Contamination
32 questions

Microbiology_5_Contamination

FrugalCombination3009 avatar
FrugalCombination3009
Microbiology Safety Practices Quiz
42 questions
Use Quizgecko on...
Browser
Browser