Microbiology Safety Practices Quiz
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Questions and Answers

Which of the following viruses is an example of a hazard group 4 microbe?

  • Chickenpox virus
  • Lassa fever virus (correct)
  • Influenza virus
  • Mumps virus

    • What is a primary control measure in a category 4 laboratory?

  • Positive pressure respirator
  • Microbiological safety cabinet
  • Chemical fume hood
  • Bio-hazard suit (correct)

    • What is the function of a High Efficiency Particulate Air (HEPA) filter in microbiological safety cabinets?

  • To filter out viruses and bacteria (correct)
  • To provide oxygen to the user
  • To maintain stable temperatures
  • To increase airflow speed

    • Which type of microbiological safety cabinet has a downflow HEPA filter?

    <p>Class II</p> Signup and view all the answers

    What is a requirement for microbiological safety cabinets used at containment level 2 and 3 during aerosol exposure activities?

    <p>They must be correctly selected and installed</p> Signup and view all the answers

    What should be avoided regarding worker positioning in a microbiological safety cabinet setting?

    <p>Having another worker at an adjacent bench</p> Signup and view all the answers

    Which of the following is NOT classified as a micro-organism?

    <p>Cellulose</p> Signup and view all the answers

    Which of the following statements about moving microbes between laboratories is true?

    <p>It is problematic.</p> Signup and view all the answers

    What characteristic distinguishes a class III microbiological safety cabinet?

    <p>It is designed for high-risk pathogens.</p> Signup and view all the answers

    What is the primary purpose of using aseptic techniques when handling micro-organisms?

    <p>To prevent contamination</p> Signup and view all the answers

    Which of the following practices should be avoided to adhere to good microbiological practice?

    <p>Wearing open-toed shoes in the laboratory</p> Signup and view all the answers

    Which equipment is necessary for work at containment level 2 and 3 when there is a risk of aerosol exposure?

    <p>Microbiological safety cabinets</p> Signup and view all the answers

    What needs to be ensured for the effective use of microbiological safety cabinets?

    <p>They must be maintained and used correctly.</p> Signup and view all the answers

    What does the Howie report focus on?

    <p>Prevention of infection in clinical labs</p> Signup and view all the answers

    What should be done immediately after using a sterilization loop?

    <p>Sterilize it in a Bunsen flame</p> Signup and view all the answers

    Which organization is NOT involved in the control of the handling of micro-organisms?

    <p>Food and Drug Administration (FDA)</p> Signup and view all the answers

    What type of shoe is considered safe in the laboratory environment?

    <p>Closed-toed shoes</p> Signup and view all the answers

    What does COSHH stand for?

    <p>Control of Substance Hazardous to Health</p> Signup and view all the answers

    What is one of the primary reasons for implementing safe working practices in a laboratory?

    <p>To reduce contact with sources of infection</p> Signup and view all the answers

    Which of the following practices helps to prevent the inhalation of airborne hazards in а laboratory?

    <p>Using proper containment equipment</p> Signup and view all the answers

    What is a known route of transmission of microbes through skin contact?

    <p>Skin puncture by needle or other sharps</p> Signup and view all the answers

    Which governing bodies are primarily responsible for laboratory safety and regulations?

    <p>Health and safety authorities</p> Signup and view all the answers

    What is the primary responsibility of an employer in terms of safety for employees working with microbial pathogens?

    <p>Ensure safety training and appropriate equipment.</p> Signup and view all the answers

    What type of injury can occur due to the ingestion route of transmission?

    <p>Infection from contaminated fingers</p> Signup and view all the answers

    Which statement about good microbiological practice is true?

    <p>It reduces the risk of contamination</p> Signup and view all the answers

    Which hazard group contains microorganisms with the highest risk to human health?

    <p>Hazard Group 4</p> Signup and view all the answers

    What is meant by 'containment' in a laboratory setting?

    <p>The use of barriers to prevent the escape of harmful substances</p> Signup and view all the answers

    Which of the following is a characteristic of Hazard Group 1 pathogens?

    <p>Have the lowest level of risk.</p> Signup and view all the answers

    What type of injury does the route of transmission through conjunctiva typically refer to?

    <p>Injury from splashes of infectious material into the eye</p> Signup and view all the answers

    What type of microorganism is Saccharomyces cerevisiae classified as?

    <p>A yeast in Hazard Group 1</p> Signup and view all the answers

    Which of the following pathogens is classified as a Hazard Group 3 microbe?

    <p>Mycobacterium tuberculosis</p> Signup and view all the answers

    Which safety measure is essential for handling microbes in a laboratory setting?

    <p>Regular disinfection of surfaces.</p> Signup and view all the answers

    Which of the following is an example of a parasite in Hazard Group 2?

    <p>Toxoplasma gondii</p> Signup and view all the answers

    Which of the following viruses is included in Hazard Group 2?

    <p>Epstein-barr virus</p> Signup and view all the answers

    Which hazard group requires police escort during transport?

    <p>Hazard Group 4</p> Signup and view all the answers

    What should the primary container for transporting microbes be made of?

    <p>Metal or plastic</p> Signup and view all the answers

    Which agency is NOT primarily involved in regulating genetic manipulation organisms (GMO)?

    <p>Environmental Protection Agency (EPA)</p> Signup and view all the answers

    What is a requirement for keeping GM risk assessments?

    <p>They should be kept for 10 years after the work ends.</p> Signup and view all the answers

    What is the primary rationale behind regulating the transport of hazardous microbes?

    <p>To ensure safety and prevent unknown risks.</p> Signup and view all the answers

    What is the main focus of health surveillance of employees in microbiology laboratories?

    <p>To detect adverse reactions to prevent health issues.</p> Signup and view all the answers

    Which of the following is NOT a characteristic of Hazard Group 4 microbes?

    <p>Have low pathogenicity.</p> Signup and view all the answers

    What primary purpose does the GM committee serve within laboratory settings?

    <p>To oversee compliance with genetic manipulation regulations.</p> Signup and view all the answers

    Study Notes

    Safe Working Practices

    • Safe working practices are crucial in all laboratories, especially in microbiology where risks may be unknown.
    • Safe working practices are highly regulated by numerous government bodies, including HSE, ACDP, NaCTSO, and DEFRA.

    Hazard Grouping

    • Microorganisms are categorized into hazard groups (HGs) based on the potential risk they pose.
    • HG1 poses the lowest risk, while HG4 poses the highest.
    • Microorganisms within the same HG are handled at the same containment level in the laboratory.

    Containment Levels

    • Containment levels (Laboratory Containment Levels, LCLs) are measures taken to prevent the spread of microbes.
    • Containment levels increase as the potential risk of the microorganism increases.
    • Laboratories handling HG1 microorganisms typically use level 1 containment. Higher-risk microorganisms (higher HGs) need higher levels of containment.

    Aseptic Techniques

    • Aseptic techniques are essential when handling microorganisms to avoid contamination of the environment, personnel, or the specimens being handled.
    • Aseptic procedures include avoiding eating, drinking, smoking, or makeup application in the lab.
    • Lab equipment, such as loops, should be sterilized after use.
    • All specimens and materials must be clearly labelled.

    Route of Microbe Transmission

    • Ingestion (eating, drinking, smoking in laboratory).
    • Skin (percutaneous) - needle punctures, animal bites, existing cuts.
    • Conjunctiva (splashes in eyes, contamination).
    • Inhalation (inhaling airborne hazards like aerosols).

    Good Microbiological Practices (GMP)

    • GMP involves appropriate handling techniques, cleanliness, and containment practices when working with microorganisms.
    • Maintaining a tidy laboratory, properly cleaning up spills, promptly sterilizing tools, labelling specimens, and adhering to containment are all essential GMP components.

    Current Legislation

    • Current legislation and regulations ensure the safe control of microorganisms.
    • Legislation on the handling of microorganisms is overseen by a number of governing bodies.
    • Government bodies like the Health and Safety Executive (HSE) and the Advisory Committee on Dangerous Pathogens (ACDP) create and enforce relevant regulations.
    • Safety reports and codes of practice generated by these agencies provide guidelines and preventative measures for safety in clinical laboratories and post-mortem rooms.

    GMO (Genetically Modified Organism)

    • Microbes' genetic makeup can change naturally (like antibiotic resistance) or intentionally in a lab setting.
    • This can require the hazard group to be adjusted (increased) based on the new potential risks.
    • GMOs are regulated by various agencies including the Scientific Advisory Committee on Genetic Modification, HSE, ACDP, DEFRA, and SSREAD.
    • GMOs are subject to additional surveillance and monitoring for potential adverse effects on health.
    • It's critical to utilize the least hazardous microbe feasible for genetic manipulation.

    Risk Assessment

    • Microorganisms are categorized into hazard groups based on their potential risks to humans.
    • Microbiological safety cabinets (MSCs), suitable for containment levels 2 and 3 work, are needed when working with organisms that pose an aerosol risk.
    • These cabinets are vital for controlling aerosolization, which is a risk of direct exposure.
    • The proper type, installation, use, and maintenance of MSCs are crucial.
    • Risk assessments should include considerations for human health, animal health, and the environment.
    • Risk assessments must be kept updated yearly and for the duration of the project.
    • Risk assessments should remain on file for 10 years after the project is completed.

    Movement of Microorganisms

    • The movement of pathogens between laboratories has historically caused problems.
    • Tightening regulations, like strict transit requirements for pathogens in risk levels 3 and 4, have been implemented for moving infectious substances.
    • Handling and transport of high-risk biological specimens require strict governmental approval.

    Category 4 Laboratories

    • Category 4 laboratories utilize secondary containment measures to further minimize the risk of exposure.
    • Bio-hazard suits, equipment separation, and stringent procedures are used to prevent accidental release and exposure to dangerous pathogens.

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    Related Documents

    Safe Working Practices & Legislation (2020) - Student Notes PDF

    Description

    Test your knowledge on safe working practices in microbiology laboratories. This quiz covers hazard grouping, containment levels, and the importance of aseptic techniques in preventing microbial contamination. Ensure you understand the regulations and guidelines for lab safety.

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