Aseptic Technique Quiz: Laboratory Practice and Procedures

Study Notes

Fundamental Rules of Aseptic Techniques

Ensure the environment is free from external sources of contamination, such as open windows or doorways.
Sterilize all tools and implements to be used.
Keep sterilized equipment easily accessible to prevent touching or leaning on surfaces.
Use a Bunsen burner to create an upward flow of air, reducing the risk of contamination.
Flame test tubes and other vessels around the cap and neck when opening to prevent contaminants from entering.
Sterilize wire loops by flaming over a Bunsen burner, heating to glowing red, to destroy microorganisms.
Use disposable pipettes and remove them from packaging without touching the tip to any surface.
Keep pots of disinfectant nearby for disposal of used items.
Transfer cultures between agar plates quickly and in a controlled manner.
Cut away from the body and dispose of sharp instruments and specimens according to instructions.

Physical Techniques

Grinding

Grinding is a method of reducing the size of hard materials or sharpening tools, often done in several stages.
Grinding is done after crushing to produce desired fineness of end products.
Grinding can be done wet or dry, depending on the process, and may require drying materials beforehand.
Various machines are used for grinding, including hand-cranked stones, handheld power tools, and industrial machines.

Drying

Drying is one of the oldest preservation processes available, used in food supply chain.
Agar plates should be opened facing away from the user and only just enough to complete the work.
Tools, clothing, and equipment can be sterilized in an autoclave, but disposable items are becoming increasingly used in critically sterile fields.

Dissection

Dissection is the process of studying internal parts of a body or plant.
Dissection is often used in scientific research, such as animal dissection.
Dissection tools and setup include:
- Placing the frog in a pan and rinsing it with water.
- Pinning the frog to secure its limbs to the pan.
- Beginning the first skin incision.

Description

Test your knowledge of fundamental rules of aseptic techniques in laboratory settings, including environment control, sterilization, and equipment handling. Learn how to minimize contamination risks and ensure a safe working environment.