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Microbiology Lab Staining Procedure

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Questions and Answers

What is the purpose of using a paper towel in the malachite green staining procedure?

To absorb excess dye
To mix the primary dye with the counterstain
To prevent the dye from evaporating too quickly (correct)
To fix the bacteria to the slide

Why is it important to remove and discard the paper towel after 5 minutes?

To allow the counterstain to bind to the bacteria
To prevent the dye from drying on the slide (correct)
To fix the bacteria to the slide
To prevent the growth of contaminants

What is the purpose of washing the slide with water after staining with malachite green?

To apply the counterstain
To remove excess dye (correct)
To decolorize the bacteria
To fix the bacteria to the slide

What is the recommended procedure for applying the counterstain safranin?

Flood the smear with safranin and leave for 1 minute (C) Signup and view all the answers

What is the purpose of using Nigrosin in the negative staining procedure?

To create a negatively charged environment for the bacteria (C) Signup and view all the answers

What is the recommended angle for holding the second slide in the negative staining procedure?

45-degree angle (D) Signup and view all the answers

Why is it important not to heat fix the slide in the negative staining procedure?

To prevent the bacteria from being fixed to the slide (A) Signup and view all the answers

What is the recommended procedure for decolorizing the bacteria?

None of the above (D) Signup and view all the answers

What is the purpose of using safranin as a counterstain?

To enhance the visibility of the bacteria under the microscope (A) Signup and view all the answers

What is the purpose of applying immersion oil to the smear?

To enhance the visibility of the bacteria under the microscope (C) Signup and view all the answers

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