10 Questions
What is the purpose of using a paper towel in the malachite green staining procedure?
To prevent the dye from evaporating too quickly
Why is it important to remove and discard the paper towel after 5 minutes?
To prevent the dye from drying on the slide
What is the purpose of washing the slide with water after staining with malachite green?
To remove excess dye
What is the recommended procedure for applying the counterstain safranin?
Flood the smear with safranin and leave for 1 minute
What is the purpose of using Nigrosin in the negative staining procedure?
To create a negatively charged environment for the bacteria
What is the recommended angle for holding the second slide in the negative staining procedure?
45-degree angle
Why is it important not to heat fix the slide in the negative staining procedure?
To prevent the bacteria from being fixed to the slide
What is the recommended procedure for decolorizing the bacteria?
None of the above
What is the purpose of using safranin as a counterstain?
To enhance the visibility of the bacteria under the microscope
What is the purpose of applying immersion oil to the smear?
To enhance the visibility of the bacteria under the microscope
Learn the steps to prepare and stain slides for microbiology lab analysis, including fixation, staining, and rinsing. Follow the procedure to ensure accurate results.
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