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10 Questions

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Microbiology Lab Staining Procedure

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Questions and Answers

What is the purpose of using a paper towel in the malachite green staining procedure?

To absorb excess dye

To mix the primary dye with the counterstain

To prevent the dye from evaporating too quickly (correct)

To fix the bacteria to the slide

Why is it important to remove and discard the paper towel after 5 minutes?

To allow the counterstain to bind to the bacteria

To prevent the dye from drying on the slide (correct)

To fix the bacteria to the slide

To prevent the growth of contaminants

What is the purpose of washing the slide with water after staining with malachite green?

To apply the counterstain

To remove excess dye (correct)

To decolorize the bacteria

To fix the bacteria to the slide

What is the recommended procedure for applying the counterstain safranin?

Flood the smear with safranin and leave for 1 minute Signup and view all the answers

What is the purpose of using Nigrosin in the negative staining procedure?

To create a negatively charged environment for the bacteria Signup and view all the answers

What is the recommended angle for holding the second slide in the negative staining procedure?

45-degree angle Signup and view all the answers

Why is it important not to heat fix the slide in the negative staining procedure?

To prevent the bacteria from being fixed to the slide Signup and view all the answers

What is the recommended procedure for decolorizing the bacteria?

None of the above Signup and view all the answers

What is the purpose of using safranin as a counterstain?

To enhance the visibility of the bacteria under the microscope Signup and view all the answers

What is the purpose of applying immersion oil to the smear?

To enhance the visibility of the bacteria under the microscope Signup and view all the answers

Microbiology Lab Staining Procedure

Choose a study mode

Podcast

Questions and Answers

What is the purpose of using a paper towel in the malachite green staining procedure?

Why is it important to remove and discard the paper towel after 5 minutes?

What is the purpose of washing the slide with water after staining with malachite green?

What is the recommended procedure for applying the counterstain safranin?

What is the purpose of using Nigrosin in the negative staining procedure?

What is the recommended angle for holding the second slide in the negative staining procedure?

Why is it important not to heat fix the slide in the negative staining procedure?

What is the recommended procedure for decolorizing the bacteria?

What is the purpose of using safranin as a counterstain?

What is the purpose of applying immersion oil to the smear?

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