Fungal Staining Techniques Quiz

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Questions and Answers

Which stain is best for filamentous fungi and yeasts, and is recommended for histoplasma found in blood and bone marrow?

  • India Ink
  • Gomori’s Methenamine Silver (GMS) Stain
  • Giemsa or Wright Stain (correct)
  • Mucicarmine Stain

Which stain is used for capsule demonstration and stains the fungal capsule red, including Cryptococcus neoformans?

  • Calcofluor White
  • Gomori’s Methenamine Silver (GMS) Stain
  • Mucicarmine Stain (correct)
  • India Ink

What is the standard culture for isolation of fungal agents, where different fungi appear differently?

  • SABOURAUD’S DEXTROSE AGAR (SDA) (correct)
  • Mucicarmine Stain
  • Calcofluor White
  • Gomori’s Methenamine Silver (GMS) Stain

Which stain is used for negative staining to highlight the heavily thickened capsule of Cryptococcus neoformans?

<p>India Ink (C)</p> Signup and view all the answers

Which stain binds to the components of the fungal cell wall specifically the chitin and cellulose, and makes it fluorescent upon using wood’s lamp?

<p>Calcofluor White (C)</p> Signup and view all the answers

Which test is helpful among immunocompetent individuals for diagnosing fungal infection?

<p>Serological testing (A)</p> Signup and view all the answers

Which fungal infection is characterized by spherules?

<p>Coccidioidomycoses (B)</p> Signup and view all the answers

Which fungal infection is associated with yeasts and pseudohyphae in skin scrapings?

<p>Pityriasis versicolor (B)</p> Signup and view all the answers

Which method is used for rapid diagnosis of fungal organisms?

<p>MALDI-TOF-MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) (B)</p> Signup and view all the answers

Which fungal infection is characterized by cysts (asci) in pulmonary specimens?

<p>Pneumocystis jirovecii (A)</p> Signup and view all the answers

Which type of mycosis affects the outermost layer of the skin or grows along hair shafts?

<p>Superficial mycosis (B)</p> Signup and view all the answers

What causes patches of hypo-or-hyperpigmentation on the neck, shoulders, chest, and back in a condition known as tinea versicolor?

<p>Malassezia furfur (B)</p> Signup and view all the answers

Which superficial mycosis is associated with 'spaghetti and meatballs' appearance under a microscope?

<p>Pityriasis versicolor (C)</p> Signup and view all the answers

What is the characteristic feature of tinea nigra, a superficial mycosis caused by Hortaea werneckii?

<p>Dark discoloration of the palm/soles (C)</p> Signup and view all the answers

Which superficial mycosis is characterized by small firm black nodules on the hair shaft?

<p>Black piedra (D)</p> Signup and view all the answers

Which group of mycoses is the only classification that is infective to other organisms?

<p>Cutaneous Mycoses (D)</p> Signup and view all the answers

Which mycosis classification involves the hair and epidermis ONLY?

<p>Superficial Mycoses (C)</p> Signup and view all the answers

Which mycosis classification usually resides in soil or on vegetation and may enter subcutaneous tissue via traumatic inoculation?

<p>Subcutaneous Mycoses (D)</p> Signup and view all the answers

Which mycosis classification is most prevalent in the world and involves the skin, hair, and nails?

<p>Cutaneous Mycoses (A)</p> Signup and view all the answers

Which mycosis classification appears in patients with a low immune system and includes Candida and Aspergillus fumigatus?

<p>Opportunistic Mycoses (D)</p> Signup and view all the answers

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