Fungal Staining Techniques Quiz
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Questions and Answers

Which stain is best for filamentous fungi and yeasts, and is recommended for histoplasma found in blood and bone marrow?

  • India Ink
  • Gomori’s Methenamine Silver (GMS) Stain
  • Giemsa or Wright Stain (correct)
  • Mucicarmine Stain
  • Which stain is used for capsule demonstration and stains the fungal capsule red, including Cryptococcus neoformans?

  • Calcofluor White
  • Gomori’s Methenamine Silver (GMS) Stain
  • Mucicarmine Stain (correct)
  • India Ink
  • What is the standard culture for isolation of fungal agents, where different fungi appear differently?

  • SABOURAUD’S DEXTROSE AGAR (SDA) (correct)
  • Mucicarmine Stain
  • Calcofluor White
  • Gomori’s Methenamine Silver (GMS) Stain
  • Which stain is used for negative staining to highlight the heavily thickened capsule of Cryptococcus neoformans?

    <p>India Ink</p> Signup and view all the answers

    Which stain binds to the components of the fungal cell wall specifically the chitin and cellulose, and makes it fluorescent upon using wood’s lamp?

    <p>Calcofluor White</p> Signup and view all the answers

    Which test is helpful among immunocompetent individuals for diagnosing fungal infection?

    <p>Serological testing</p> Signup and view all the answers

    Which fungal infection is characterized by spherules?

    <p>Coccidioidomycoses</p> Signup and view all the answers

    Which fungal infection is associated with yeasts and pseudohyphae in skin scrapings?

    <p>Pityriasis versicolor</p> Signup and view all the answers

    Which method is used for rapid diagnosis of fungal organisms?

    <p>MALDI-TOF-MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry)</p> Signup and view all the answers

    Which fungal infection is characterized by cysts (asci) in pulmonary specimens?

    <p>Pneumocystis jirovecii</p> Signup and view all the answers

    Which type of mycosis affects the outermost layer of the skin or grows along hair shafts?

    <p>Superficial mycosis</p> Signup and view all the answers

    What causes patches of hypo-or-hyperpigmentation on the neck, shoulders, chest, and back in a condition known as tinea versicolor?

    <p>Malassezia furfur</p> Signup and view all the answers

    Which superficial mycosis is associated with 'spaghetti and meatballs' appearance under a microscope?

    <p>Pityriasis versicolor</p> Signup and view all the answers

    What is the characteristic feature of tinea nigra, a superficial mycosis caused by Hortaea werneckii?

    <p>Dark discoloration of the palm/soles</p> Signup and view all the answers

    Which superficial mycosis is characterized by small firm black nodules on the hair shaft?

    <p>Black piedra</p> Signup and view all the answers

    Which group of mycoses is the only classification that is infective to other organisms?

    <p>Cutaneous Mycoses</p> Signup and view all the answers

    Which mycosis classification involves the hair and epidermis ONLY?

    <p>Superficial Mycoses</p> Signup and view all the answers

    Which mycosis classification usually resides in soil or on vegetation and may enter subcutaneous tissue via traumatic inoculation?

    <p>Subcutaneous Mycoses</p> Signup and view all the answers

    Which mycosis classification is most prevalent in the world and involves the skin, hair, and nails?

    <p>Cutaneous Mycoses</p> Signup and view all the answers

    Which mycosis classification appears in patients with a low immune system and includes Candida and Aspergillus fumigatus?

    <p>Opportunistic Mycoses</p> Signup and view all the answers

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