AFB Stain Procedure Components Quiz

True or False: Filter paper should be dry during heating for proper penetration of the primary stain.

True or False: Coplin Jars are recommended for AFB staining due to their effectiveness in holding potential acid-fast bacilli.

True or False: Acid-fast organisms will appear blue when viewed under a microscope.

True or False: Mycolic acid forms a lipid shell around the organism that makes it more permeable.

True or False: A minimum of 500 fields should be viewed before declaring a slide negative in AFB staining.

True or False: Fuchsin is the secondary stain used in the Ziehl-Neelsen AFB staining procedure.

It is recommended to rinse the slide with alcohol after adding a few drops of decolorizer.

The purpose of stopping the decolorizer when the solvent is no longer colored as it flows over the slide is to avoid excess decolorization in gram-positive cells.

Basic fuchsin is used as a primary stain in the Gram staining procedure.

Acid-fastness is a characteristic feature of the genus Mycobacteria due to their ability to withstand strong acid decolorizers.

The concept of acid-fast staining is mentioned in the context of spot-AM collection techniques.

Bacteriolysis is an essential step in the acid-fast staining process.

Non-acid fast organisms can be easily decolorized with acid alcohol.

Acid-fastness can be observed (albeit weakly) in Nocardiella spp.

Brushing your teeth before sputum sample collection is recommended.

It is advisable to drink coffee or tea before sample collection.

The first spot specimen should be collected at the time of the first consultation.

Sputum sample for Xpert MTB/RIF should not be less than 5 ml.