Podcast
Questions and Answers
True or False: Filter paper should be dry during heating for proper penetration of the primary stain.
True or False: Filter paper should be dry during heating for proper penetration of the primary stain.
False
True or False: Coplin Jars are recommended for AFB staining due to their effectiveness in holding potential acid-fast bacilli.
True or False: Coplin Jars are recommended for AFB staining due to their effectiveness in holding potential acid-fast bacilli.
False
True or False: Acid-fast organisms will appear blue when viewed under a microscope.
True or False: Acid-fast organisms will appear blue when viewed under a microscope.
False
True or False: Mycolic acid forms a lipid shell around the organism that makes it more permeable.
True or False: Mycolic acid forms a lipid shell around the organism that makes it more permeable.
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True or False: A minimum of 500 fields should be viewed before declaring a slide negative in AFB staining.
True or False: A minimum of 500 fields should be viewed before declaring a slide negative in AFB staining.
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True or False: Fuchsin is the secondary stain used in the Ziehl-Neelsen AFB staining procedure.
True or False: Fuchsin is the secondary stain used in the Ziehl-Neelsen AFB staining procedure.
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It is recommended to rinse the slide with alcohol after adding a few drops of decolorizer.
It is recommended to rinse the slide with alcohol after adding a few drops of decolorizer.
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The purpose of stopping the decolorizer when the solvent is no longer colored as it flows over the slide is to avoid excess decolorization in gram-positive cells.
The purpose of stopping the decolorizer when the solvent is no longer colored as it flows over the slide is to avoid excess decolorization in gram-positive cells.
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Basic fuchsin is used as a primary stain in the Gram staining procedure.
Basic fuchsin is used as a primary stain in the Gram staining procedure.
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Acid-fastness is a characteristic feature of the genus Mycobacteria due to their ability to withstand strong acid decolorizers.
Acid-fastness is a characteristic feature of the genus Mycobacteria due to their ability to withstand strong acid decolorizers.
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The concept of acid-fast staining is mentioned in the context of spot-AM collection techniques.
The concept of acid-fast staining is mentioned in the context of spot-AM collection techniques.
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Bacteriolysis is an essential step in the acid-fast staining process.
Bacteriolysis is an essential step in the acid-fast staining process.
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Non-acid fast organisms can be easily decolorized with acid alcohol.
Non-acid fast organisms can be easily decolorized with acid alcohol.
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Acid-fastness can be observed (albeit weakly) in Nocardiella spp.
Acid-fastness can be observed (albeit weakly) in Nocardiella spp.
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Brushing your teeth before sputum sample collection is recommended.
Brushing your teeth before sputum sample collection is recommended.
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It is advisable to drink coffee or tea before sample collection.
It is advisable to drink coffee or tea before sample collection.
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The first spot specimen should be collected at the time of the first consultation.
The first spot specimen should be collected at the time of the first consultation.
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Sputum sample for Xpert MTB/RIF should not be less than 5 ml.
Sputum sample for Xpert MTB/RIF should not be less than 5 ml.
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