Questions and Answers
What is the purpose of the Phenylalanine Deaminase lab?
To detect organisms which are capable of producing phenylalanine deaminase
What type of media was used for the phenylalanine deaminase lab?
Phenylalanine slants
What chemical reagent was added to the phenylalanine deaminase lab and why was it added?
FeCl3 or Ferric chloride. It helps determine if phenylalanine deaminase is produced by being a visual indicator.
When was FeCl3 added?
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What does the result to the left mean if the left image is yellow?
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What are the ingredients for this lab?
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How long does this lab need to be incubated for?
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Why is FeCl3 added to the agar?
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Is this lab selective or differential reagent?
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What is the function of phenylalanine deaminase?
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Which genera of bacteria can produce this enzyme?
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What disease is associated with these genera?
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What is the name of the amino acid provided in the agar?
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What is deamination?
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What is decarboxylation?
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Phenylalanine is broken down to?
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Explain what a positive result looks like and what it means?
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What other experiment will we perform with deaminase activity?
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Why isn't mineral oil added?
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Can an organism still grow in this media but test negative for phenylalanine deaminase?
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What is the end product that is being detected for this lab?
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Why are enzymes or biochemical reactions needed?
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What is enzyme composition?
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Enzymes interact with what?
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What are the functions of enzymes?
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What is the suffix for enzyme names?
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What is protein catabolism?
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What does an amino acid structure contain?
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What biochemical group is being removed from the phenylalanine deaminase lab?
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How would a positive result look like and what does it mean?
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What media was used for this lab?
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How does the biochemical reaction of phenylpyruvic acid look like?
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Explain what is happening in the biochemical reaction for phenylalanine deaminase lab.
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Study Notes
Phenylalanine Deaminase Lab Overview
- Aimed at detecting organisms capable of producing phenylalanine deaminase.
- Utilizes phenylalanine slants as culture media.
Reagents and Procedures
- Ferric chloride (FeCl3) is added post-incubation to serve as a visual indicator for enzyme production.
- Incubation lasts for 24 hours on the surface of the slant before adding FeCl3.
- A negative result shows no color change (yellow), indicating no phenylalanine deaminase production.
Ingredients and Chemical Processes
- Media consists of yeast extract, phenylalanine, salt, and agar.
- Deamination involves the removal of the amino group (-NH2) from phenylalanine, producing ammonia and phenylpyruvic acid.
- Decarboxylation, an anaerobic process, removes the carboxyl group (-COOH) and can lower pH.
Interpretation of Results
- A dark green slant result signifies a positive test, indicating phenylalanine was deaminated to phenylpyruvic acid, confirmed by FeCl3 reaction.
- Organisms can grow in the media but may test negative for phenylalanine deaminase.
Associated Bacteria and Diseases
- Genera able to produce the enzyme include Proteus (associated with UTIs), Morganella, and Providencia which causes opportunistic infections.
Additional Information
- Enzymes are proteins that catalyze biochemical reactions, often ending with the suffix "-ase."
- Enzymatic reactions require specific substrates and facilitate processes such as digestion and metabolism.
- Amino acid structures consist of a central carbon, an amino group, a carboxyl group, and a variable side chain.
Related Experiments
- Further experiments involving deaminase activity can be performed using Lysine Iron Agar.
- Mineral oil is not added since it results in inhibiting necessary oxygen during the deamination process.
Understanding Phenylalanine Pathway
- The biochemical reaction transforms phenylalanine into phenylpyruvic acid, highlighting the organism's enzymatic capability.
- The end product of interest is phenylpyruvic acid, essential for identifying microbial characteristics in this lab.
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Description
This quiz focuses on the Phenylalanine Deaminase lab, specifically designed to assess the understanding of its purpose, the media used, and the reagents involved. It includes crucial terminology and definitions that are vital for microbiology studies.