Microbiology: Gram Staining and Bacterial Characteristics
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Questions and Answers

What is the purpose of the crystal violet dye in the gram stain process?

  • To differentiate between gram-positive and gram-negative bacteria (correct)
  • To stain the bacteria purple
  • To decolorize the bacteria
  • To remove excess iodine solution
  • What is the primary difference between gram-positive and gram-negative bacteria?

  • Their reaction to the gram stain process (correct)
  • Their cell wall structure
  • Their ability to form spores
  • Their ability to grow in the presence of oxygen
  • What is the term for bacteria that require oxygen to grow?

  • Anaerobes
  • Aerobes (correct)
  • Obligate aerobes
  • Facultative anaerobes
  • What is the function of the capsule in bacteria?

    <p>To enhance the bacterium's ability to resist the body's defense mechanisms</p> Signup and view all the answers

    What is the term for bacteria that can grow in the presence or absence of oxygen?

    <p>Facultative anaerobes</p> Signup and view all the answers

    What is the term for the highly resistant form of bacteria that can survive extreme conditions?

    <p>Spores</p> Signup and view all the answers

    What is the term for single-cell organisms with complex internal structures?

    <p>Protozoa</p> Signup and view all the answers

    What is the term for the thick-walled resting stage of protozoa that can survive outside a host?

    <p>Cysts</p> Signup and view all the answers

    What is the term for a type of fungus that causes opportunistic infections, such as oral candidiasis?

    <p>Candida albicans</p> Signup and view all the answers

    What is the primary cause of genital lesions?

    <p>Herpes simplex virus type 2</p> Signup and view all the answers

    Which virus is transmitted through the bite of an infectious mosquito?

    <p>Zika Virus</p> Signup and view all the answers

    What is the primary effect of the West Nile virus on the human body?

    <p>Inflammation of the brain and spinal cord</p> Signup and view all the answers

    What is the primary purpose of surface barriers?

    <p>To protect surfaces from becoming contaminated</p> Signup and view all the answers

    What is a disadvantage of using surface barriers?

    <p>They add plastics to the environment</p> Signup and view all the answers

    What is the primary mode of transmission of the Ebola virus?

    <p>Direct contact of broken skin or mucous membrane with blood and bodily fluids</p> Signup and view all the answers

    What is the benefit of precleaning and disinfecting surfaces between patients?

    <p>It may be less expensive than using surface barriers</p> Signup and view all the answers

    What is the primary effect of the H1N1 Flu virus on the human body?

    <p>Respiratory disease</p> Signup and view all the answers

    What is the primary mode of transmission of the Cytomegalovirus?

    <p>Through most body fluids</p> Signup and view all the answers

    What is a challenge of precleaning and disinfecting surfaces?

    <p>It is a time-consuming process</p> Signup and view all the answers

    What is the primary effect of the Herpes Zoster virus on the human body?

    <p>Causes herpes zoster or chickenpox and shingles</p> Signup and view all the answers

    Why is it important to clean and disinfect surfaces in a dental setting?

    <p>To reduce the risk of infection</p> Signup and view all the answers

    What is the primary mode of transmission of the Epstein-Barr Virus?

    <p>Through most body fluids</p> Signup and view all the answers

    What is the purpose of precleaning in a dental setting?

    <p>To remove bioburden</p> Signup and view all the answers

    How long should a person wait after returning to North America before seeking dental treatment if they have been exposed to the Ebola virus?

    <p>21 days</p> Signup and view all the answers

    Why is it important to use a variety of shapes and sizes of surface barriers?

    <p>To ensure a proper fit on all surfaces</p> Signup and view all the answers

    What is a challenge of using surface barriers during dental treatment?

    <p>They may become dislodged during treatment</p> Signup and view all the answers

    What is an advantage of using surface barriers in a dental setting?

    <p>They reduce the handling and storage of chemicals</p> Signup and view all the answers

    What type of disinfectant is capable of killing spores?

    <p>Sporicidal</p> Signup and view all the answers

    What is the purpose of the first step in the 7-step process of instrument processing?

    <p>To transport contaminated instruments to the processing area</p> Signup and view all the answers

    What type of disinfectant is effective against a wide variety of microbes?

    <p>Synthetic phenol compounds</p> Signup and view all the answers

    What is the purpose of using a holding solution?

    <p>To temporarily store instruments before cleaning</p> Signup and view all the answers

    Why is alcohol not used in dentistry?

    <p>It is ineffective at removing bioburden</p> Signup and view all the answers

    What is the purpose of using a hands-free, mechanical process in instrument cleaning?

    <p>To reduce the risk of exposure to persons and the environment</p> Signup and view all the answers

    What type of disinfectant is an example of an intermediate level disinfectant?

    <p>Iodophors</p> Signup and view all the answers

    What is the purpose of sterilization in instrument processing?

    <p>To completely eliminate all microorganisms from instruments</p> Signup and view all the answers

    What is the primary purpose of using a rigid leak-proof container in instrument transportation?

    <p>To reduce the risk of exposure to persons and the environment</p> Signup and view all the answers

    What is the primary use of mouth mirrors and amalgam condensers?

    <p>For cleaning and basic disinfection procedures</p> Signup and view all the answers

    What is the purpose of a unidirectional flow in an instrument processing area?

    <p>To prevent cross-contamination</p> Signup and view all the answers

    What is the advantage of using steam autoclave for sterilization?

    <p>It does not corrode instruments</p> Signup and view all the answers

    What is the disadvantage of using unsaturated chemical vapour for sterilization?

    <p>It damages some plastic and rubber items</p> Signup and view all the answers

    What is the advantage of using a dry heat oven for sterilization?

    <p>It does not corrode instruments</p> Signup and view all the answers

    What is the purpose of using process integrators or multi-parameter indicators in sterilization monitoring?

    <p>To respond to the combination of pressure, temperature, and time</p> Signup and view all the answers

    What is the advantage of using rapid heat transfer for sterilization?

    <p>It is a very fast process</p> Signup and view all the answers

    What is the purpose of ultrasonic cleaner testing with 5x5 lightweight aluminum foil?

    <p>To test the effectiveness of the ultrasonic cleaner</p> Signup and view all the answers

    What is the disadvantage of using steam autoclave for sterilization of non-stainless steel instruments?

    <p>It may rust non-stainless steel instruments</p> Signup and view all the answers

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