Microbiology Culture Media Quiz

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What is the primary use of Bacteroides bile-esculin agar (BBE)?

Enrichment for fastidious anaerobes
Isolation and identification of *Bacteroides fragilis* (correct)
Selective medium for *Clostridium difficile*
Isolation of *Corynebacterium diphtheriae*

Which medium is selective for the isolation of Clostridium difficile?

Blood agar, anaerobic, CDC
Thioglycollate broth (THIO)
Cycloserine cefoxitin fructose egg yolk agar (CCFA) (correct)
Cooked meat medium

What characteristic indicates hydrolysis of esculin in Bacteroides bile-esculin agar?

Blackening of the agar (correct)
Production of gas bubbles
Formation of a yellow color
Presence of a metallic sheen

What does blood agar, anaerobic, CDC primarily support?

Isolation of pathogenic Clostridium (C) Signup and view all the answers

Which medium promotes the growth of Corynebacterium diphtheriae and indicates colony formation?

Cystine-tellurite blood agar (C) Signup and view all the answers

Which medium is primarily used for the isolation of Salmonella?

Bismuth sulfite agar (D) Signup and view all the answers

What does alkaline peptone water primarily target for recovery?

Vibrio and Aeromonas from stool (C) Signup and view all the answers

In which medium do S. typhi colonies appear black with a metallic sheen?

Bismuth sulfite agar (C) Signup and view all the answers

What is the function of bordet-Gengou medium?

Enrichment for Bordetella pertussis (A) Signup and view all the answers

What type of medium is Phenylethyl alcohol agar (PEA) considered?

All-purpose medium for aerobes and anaerobes (C) Signup and view all the answers

Bacteroides bile-esculin agar (BBE)

Selective differential medium for isolating Bacteroides fragilis. Bile salts and gentamicin inhibit other bacteria, while esculin hydrolysis causes blackening of the agar.

Blood agar, anaerobic, CDC

Enrichment medium for isolating fastidious anaerobes, especially pathogenic Clostridium. Contains yeast extract, L-cysteine, hemin, and vitamin K. Helps determine lecithinase and lipase production by bacteria.

Cycloserine cefoxitin fructose egg yolk agar (CCFA)

Selective for isolating a common intestinal pathogen, Clostridium difficile.

Cystine-tellurite blood agar

Selective for isolating Corynebacterium diphtheriae. Black colonies indicate growth of the bacteria.