Bacterial Culture Media Quiz

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Questions and Answers

Which type of agar is specifically designed for isolating Pseudomonas aeruginosa?

Hektoen enteric agar
Mannitol salt agar
Cetrimide agar (correct)
Bile esculin agar

What is the primary purpose of Hektoen enteric agar?

Isolate fecal bacteria from the Enterobacteriaceae family (correct)
Detect halophiles that ferment mannitol
Isolate lactose-fermenting bacteria
Differentiate urinary tract bacteria

CLED agar is primarily used to differentiate which types of bacteria?

Lactose-fermenting and non-lactose fermenting bacteria (correct)
Enterococci and Streptococci
Halophilic and non-halophilic bacteria
Gram-positive and Gram-negative bacteria

Which agar is used to isolate Enterococci and group D Streptococci?

<p>Bile esculin agar (D)</p> Signup and view all the answers

Mannitol salt agar is specifically effective in isolating which type of organisms?

<p>Halophiles that ferment mannitol (D)</p> Signup and view all the answers

Flashcards

Bile esculin agar

A selective medium used to isolate Enterococci and group D Streptococci.

Cetrimide agar

A selective medium used to isolate Pseudomonas aeruginosa, a common cause of infections.

CLED agar

A differential medium used to isolate and differentiate urinary tract bacteria. It inhibits the growth of Proteus species and allows for the identification of lactose fermenting bacteria based on their ability to produce acid and change the color of the medium.

Hektoen Enteric Agar (HEA)

A selective and differential medium used to isolate and identify Salmonella and Shigella species from fecal samples.

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Mannitol Salt Agar (MSA)

A selective and differential medium used to isolate and identify Staphylococcus aureus, a common cause of skin infections. It contains high salt concentration and the sugar mannitol. S. aureus ferments mannitol producing acid, which lowers the pH and turns the indicator yellow.

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Study Notes

Bacterial Culture Media

Bile esculin agar isolates Enterococci and group D Streptococci.
Cetrimide agar isolates Pseudomonas aeruginosa.
Cysteine-lactose electrolyte deficient (CLED) agar isolates and differentiates urinary tract bacteria. It inhibits Proetus species swarming, and differentiates lactose fermenters from non-lactose fermenters.
Hektoen enteric agar (HEA) isolates and recovers fecal bacteria of the Enterobacteriaceae family, particularly Salmonella and Shigella species.
Mannitol salt agar (MSA) isolates organisms, especially halophiles, that ferment mannitol, producing lactic acid.

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Description

Test your knowledge on various bacterial culture media used in microbiology. This quiz covers specific media like Bile esculin agar, Cetrimide agar, and Mannitol salt agar, and their applications in isolating different bacteria. Perfect for students and professionals in the field of microbiology.