Microbiology Colony Morphology Quiz
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Questions and Answers

What is the Gram stain result for Aeromonas?

  • Gram-positive
  • Gram-negative (correct)
  • Gram-variable
  • Does not stain with Gram stain
  • Which bacterial strain produces smooth, convex, translucent colonies?

  • C. mucosalis
  • C. fetus subsp. fetus (correct)
  • Aeromonas
  • Helicobacter spp.
  • What is the definitive identification test for Campylobacter jejuni?

  • Negative urease activity test
  • Negative oxidase test
  • Positive hippurate hydrolysis test (correct)
  • Negative hippurate hydrolysis test
  • Which bacterial strain demonstrates 'darting' motility?

    <p>Campylobacter</p> Signup and view all the answers

    What is the culture medium used for definitive identification of H. pylori?

    <p>Christensen’s urea medium at 37°C</p> Signup and view all the answers

    Which indirect test can be used to detect the presence of H. pylori?

    <p>Urea Breath Test</p> Signup and view all the answers

    Which characteristic is unique to Pseudomonas stutzeri for differentiating it from other Pseudomonas species?

    <p>Wrinkled, leathery, adherent colonies</p> Signup and view all the answers

    What characteristic is common to Pseudomonas mendocina and Pseudomonas pseudoalcaligenes/alciligenes?

    <p>Positive for oxidase and ADH</p> Signup and view all the answers

    What is a distinguishing feature of Pseudomonas mendocina compared to other Pseudomonas species?

    <p>Production of pyoverdine</p> Signup and view all the answers

    What property can be used to differentiate Pseudomonas pseudoalcaligenes from other Pseudomonas species?

    <p>Weak ferment fructose</p> Signup and view all the answers

    Which feature is indicative of Pseudomonas mosellii among the given options?

    <p>Optimal growth at 30°C</p> Signup and view all the answers

    What characteristic of Pseudomonas nonfluorescent group helps in distinguishing it from other Pseudomonas species?

    <p>Light-yellow or brown pigment</p> Signup and view all the answers

    What causes urethritis?

    <p>Neisseria gonorrhoeae, Chlamydia trachomatis, or Ureaplasma urealyticum</p> Signup and view all the answers

    What is the microscopic morphology associated with gonococcal urethritis?

    <p>Gram-negative intracellular diplococci</p> Signup and view all the answers

    What is the recommended culture medium for identifying Neisseria gonorrhoeae?

    <p>Chocolate Agar Plate</p> Signup and view all the answers

    Which biochemical test is used for definitive identification of Neisseria gonorrhoeae?

    <p>Oxidase Test</p> Signup and view all the answers

    What color change indicates a positive result in the oxidase test for Neisseria gonorrhoeae?

    <p>Purple within 10 seconds</p> Signup and view all the answers

    Which of the following is NOT a recommended specimen for direct microscopic examination?

    <p>Pharyngeal swab</p> Signup and view all the answers

    Study Notes

    Gram Stain and Identification

    • Aeromonas results in a Gram-negative stain.
    • Campylobacter jejuni is definitively identified using the oxidase test.
    • A bacterial strain producing smooth, convex, translucent colonies is typically associated with Staphylococcus aureus.
    • 'Darting' motility is exhibited by Campylobacter species.

    Culture Media and Tests

    • The culture medium for identifying Helicobacter pylori is typically Campylobacter blood-free selective media.
    • Indirect tests for detecting Helicobacter pylori presence include serological tests for antibodies.
    • Neisseria gonorrhoeae is identified using Thayer-Martin agar.

    Pseudomonas Characteristics

    • Pseudomonas stutzeri is uniquely differentiated by its ability to produce yellow colonies and its negative reaction to oxidase.
    • Pseudomonas mendocina and Pseudomonas pseudoalcaligenes/alcaligenes are commonly characterized by their greenish pigmentation on culture.
    • Pseudomonas mendocina is distinguished by its production of a specific brown pigment.
    • Pseudomonas pseudoalcaligenes can be differentiated by being nitrate-reducing.
    • Pseudomonas mosellii is noted for its ability to produce a sweet odor.

    Urethritis and Gonorrhea

    • Urethritis can be caused by various pathogens, including Neisseria gonorrhoeae.
    • Microscopic morphology in gonococcal urethritis reveals Gram-negative diplococci within neutrophils.
    • The recommended culture medium for Neisseria gonorrhoeae is Chocolate agar.
    • The oxidase test for Neisseria gonorrhoeae shows a color change to dark purple for a positive result.
    • Specimens not recommended for direct microscopic examination include throat swabs.

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    Test your knowledge on identifying bacteria based on colony morphology characteristics such as color, shape, and texture. Questions cover different types of colonies produced by bacteria like runny, spreading, and pigmented colonies.

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