Microbiology Colony Morphology Quiz
18 Questions
17 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the Gram stain result for Aeromonas?

  • Gram-positive
  • Gram-negative (correct)
  • Gram-variable
  • Does not stain with Gram stain

Which bacterial strain produces smooth, convex, translucent colonies?

  • C. mucosalis
  • C. fetus subsp. fetus (correct)
  • Aeromonas
  • Helicobacter spp.

What is the definitive identification test for Campylobacter jejuni?

  • Negative urease activity test
  • Negative oxidase test
  • Positive hippurate hydrolysis test (correct)
  • Negative hippurate hydrolysis test

Which bacterial strain demonstrates 'darting' motility?

<p>Campylobacter (D)</p> Signup and view all the answers

What is the culture medium used for definitive identification of H. pylori?

<p>Christensen’s urea medium at 37°C (C)</p> Signup and view all the answers

Which indirect test can be used to detect the presence of H. pylori?

<p>Urea Breath Test (A)</p> Signup and view all the answers

Which characteristic is unique to Pseudomonas stutzeri for differentiating it from other Pseudomonas species?

<p>Wrinkled, leathery, adherent colonies (A)</p> Signup and view all the answers

What characteristic is common to Pseudomonas mendocina and Pseudomonas pseudoalcaligenes/alciligenes?

<p>Positive for oxidase and ADH (A)</p> Signup and view all the answers

What is a distinguishing feature of Pseudomonas mendocina compared to other Pseudomonas species?

<p>Production of pyoverdine (B)</p> Signup and view all the answers

What property can be used to differentiate Pseudomonas pseudoalcaligenes from other Pseudomonas species?

<p>Weak ferment fructose (A)</p> Signup and view all the answers

Which feature is indicative of Pseudomonas mosellii among the given options?

<p>Optimal growth at 30°C (D)</p> Signup and view all the answers

What characteristic of Pseudomonas nonfluorescent group helps in distinguishing it from other Pseudomonas species?

<p>Light-yellow or brown pigment (B)</p> Signup and view all the answers

What causes urethritis?

<p>Neisseria gonorrhoeae, Chlamydia trachomatis, or Ureaplasma urealyticum (C)</p> Signup and view all the answers

What is the microscopic morphology associated with gonococcal urethritis?

<p>Gram-negative intracellular diplococci (A)</p> Signup and view all the answers

What is the recommended culture medium for identifying Neisseria gonorrhoeae?

<p>Chocolate Agar Plate (A)</p> Signup and view all the answers

Which biochemical test is used for definitive identification of Neisseria gonorrhoeae?

<p>Oxidase Test (B)</p> Signup and view all the answers

What color change indicates a positive result in the oxidase test for Neisseria gonorrhoeae?

<p>Purple within 10 seconds (D)</p> Signup and view all the answers

Which of the following is NOT a recommended specimen for direct microscopic examination?

<p>Pharyngeal swab (C)</p> Signup and view all the answers

Flashcards

Aeromonas Gram stain

Gram-negative

C. fetus subsp. fetus colonies

Smooth, convex, translucent colonies

Campylobacter jejuni identification

Positive hippurate hydrolysis test

Campylobacter motility

Darting motility

Signup and view all the flashcards

H. pylori identification medium

Christensen’s urea medium at 37°C

Signup and view all the flashcards

H. pylori detection (indirect)

Urea Breath Test

Signup and view all the flashcards

Pseudomonas stutzeri colony

Wrinkled, leathery, adherent colonies

Signup and view all the flashcards

Pseudomonas mendocina/pseudoalcaligenes/alciligenes

Positive for oxidase and ADH

Signup and view all the flashcards

Pseudomonas mendocina feature

Production of pyoverdine

Signup and view all the flashcards

Pseudomonas pseudoalcaligenes/alciligenes trait

Weak ferment fructose

Signup and view all the flashcards

Pseudomonas mosellii growth

Optimal growth at 30°C

Signup and view all the flashcards

Pseudomonas nonfluorescent group

Light-yellow or brown pigment

Signup and view all the flashcards

Urethritis cause

Neisseria gonorrhoeae, Chlamydia trachomatis, or Ureaplasma urealyticum

Signup and view all the flashcards

Gonococcal urethritis morphology

Gram-negative intracellular diplococci

Signup and view all the flashcards

Neisseria gonorrhoeae culture

Chocolate Agar Plate

Signup and view all the flashcards

Neisseria gonorrhoeae identification test

Oxidase Test

Signup and view all the flashcards

Oxidase Test positive color

Purple within 10 seconds

Signup and view all the flashcards

Inappropriate specimen for direct microscopic exam

Pharyngeal swab

Signup and view all the flashcards

Study Notes

Gram Stain and Identification

  • Aeromonas results in a Gram-negative stain.
  • Campylobacter jejuni is definitively identified using the oxidase test.
  • A bacterial strain producing smooth, convex, translucent colonies is typically associated with Staphylococcus aureus.
  • 'Darting' motility is exhibited by Campylobacter species.

Culture Media and Tests

  • The culture medium for identifying Helicobacter pylori is typically Campylobacter blood-free selective media.
  • Indirect tests for detecting Helicobacter pylori presence include serological tests for antibodies.
  • Neisseria gonorrhoeae is identified using Thayer-Martin agar.

Pseudomonas Characteristics

  • Pseudomonas stutzeri is uniquely differentiated by its ability to produce yellow colonies and its negative reaction to oxidase.
  • Pseudomonas mendocina and Pseudomonas pseudoalcaligenes/alcaligenes are commonly characterized by their greenish pigmentation on culture.
  • Pseudomonas mendocina is distinguished by its production of a specific brown pigment.
  • Pseudomonas pseudoalcaligenes can be differentiated by being nitrate-reducing.
  • Pseudomonas mosellii is noted for its ability to produce a sweet odor.

Urethritis and Gonorrhea

  • Urethritis can be caused by various pathogens, including Neisseria gonorrhoeae.
  • Microscopic morphology in gonococcal urethritis reveals Gram-negative diplococci within neutrophils.
  • The recommended culture medium for Neisseria gonorrhoeae is Chocolate agar.
  • The oxidase test for Neisseria gonorrhoeae shows a color change to dark purple for a positive result.
  • Specimens not recommended for direct microscopic examination include throat swabs.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Description

Test your knowledge on identifying bacteria based on colony morphology characteristics such as color, shape, and texture. Questions cover different types of colonies produced by bacteria like runny, spreading, and pigmented colonies.

More Like This

Use Quizgecko on...
Browser
Browser