Microbiology: Coliforms and Fecal Coliforms in Food
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Questions and Answers

What is the primary reason why coliforms are used as indicators of contamination?

  • They are the primary pathogens in foodborne illnesses
  • They are the most common type of bacteria found in food
  • They react similarly to pathogens in the natural environment (correct)
  • They are easily cultured and identified in a laboratory setting
  • What is the term for all members of the coliform bacteria group?

  • Klebsiella
  • Fecal coliforms
  • Total coliforms (correct)
  • Enterobacter
  • Which of the following foods is a common source of coliforms?

  • Pasteurized milk
  • White brined cheese (correct)
  • Cooked poultry
  • Canned vegetables
  • What is the characteristic of coliforms that allows them to be used as indicators of contamination?

    <p>Their ability to ferment lactose</p> Signup and view all the answers

    What is the primary habitat of coliforms?

    <p>Soil and vegetation</p> Signup and view all the answers

    What is the implication of detecting coliforms in food?

    <p>The food is contaminated with other pathogenic organisms</p> Signup and view all the answers

    What is the primary function of the tetrazolium indicator in the Petrifilm EC/Coliform Count Plate?

    <p>To facilitate colony enumeration</p> Signup and view all the answers

    Why is it essential to perform the presumptive test before the confirmatory test in coliform detection?

    <p>To ensure the statistical validity of the method</p> Signup and view all the answers

    What is the purpose of the 5-bromo-4-chloro-3-indolyl-D-glucuronide (BCIG) in the Petrifilm EC/Coliform Count Plate?

    <p>To detect the presence of glucuronidase activity</p> Signup and view all the answers

    What is the primary advantage of using the Petrifilm EC/Coliform Count Plate in food and beverage industries?

    <p>It reduces the time required for testing</p> Signup and view all the answers

    What is the significance of Modified Violet Red Bile (VRB) nutrients in the Petrifilm EC/Coliform Count Plate?

    <p>It inhibits the growth of non-coliform bacteria</p> Signup and view all the answers

    What is the primary implication of coliform contamination in food and beverages?

    <p>All of the above</p> Signup and view all the answers

    What is the primary habitat of Escherichia coli bacteria?

    <p>Lower part of the intestines of humans and animals</p> Signup and view all the answers

    What is the function of 0.1% peptone water in the detection of coliforms?

    <p>It is a minimal nutrient media designed to reduce multiplication of microorganisms</p> Signup and view all the answers

    What is the significance of detecting fecal coliforms in food and water?

    <p>It indicates the presence and risk of pathogenic microorganisms</p> Signup and view all the answers

    What is the purpose of using Lauryl sulfate tryptose broth (LSTB) in the detection of coliforms?

    <p>It is used for the presumptive testing for the presence of coliforms</p> Signup and view all the answers

    What is the characteristic of Escherichia coli that makes it a member of the thermotolerant coliform group?

    <p>It can ferment lactose in 48 hours at 44.5ºC</p> Signup and view all the answers

    What is the significance of recovering fecal coliforms from processed food?

    <p>It indicates post-sanitation and post-process contaminations</p> Signup and view all the answers

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