Microbiology Chapter 5 Flashcards
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Microbiology Chapter 5 Flashcards

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Questions and Answers

What is metabolism?

The chemical reactions in the cell that obtain/utilize energy, breakdown compounds to obtain building blocks for synthesis, or conduct synthesis of cellular compounds & structures.

What is the need for metabolism?

Precursor molecules and energy (electron source/flow).

What is anabolism?

Synthesis.

What is catabolism?

<p>Breakdown of compounds to obtain energy &amp; building blocks.</p> Signup and view all the answers

What are oxidation-reduction cycles?

<p>Movement of electrons/energy about the cell.</p> Signup and view all the answers

What are electron carriers?

<p>NAD, NADP, FAD.</p> Signup and view all the answers

What is ATP?

<p>An energy molecule used for synthesis, active transport, and cell movement.</p> Signup and view all the answers

What are enzymes?

<p>Proteins with Primary to Quaternary level structures.</p> Signup and view all the answers

What is a substrate?

<p>The compound that an enzyme acts upon.</p> Signup and view all the answers

What effects enzymes?

<p>Temperature and pH.</p> Signup and view all the answers

What is fermentation?

<p>A metabolic process that begins with pyruvic acid, less efficient than respiration.</p> Signup and view all the answers

The primary structure of proteins is the sequence of __________.

<p>amino acids.</p> Signup and view all the answers

The process by which glucose is broken down in the Krebs cycle produces __________ ATPs.

<ol start="38"> <li></li> </ol> Signup and view all the answers

Which of the following are electron carriers?

<p>NAD</p> Signup and view all the answers

Which of the following is a result of enzyme activity?

<p>Increase in reaction rate</p> Signup and view all the answers

What do carbohydrates catabolism involve?

<p>Conversion of glucose into amino acids, sugar, fats, etc.</p> Signup and view all the answers

What is feedback inhibition?

<p>Product of enzyme action returns to earlier steps and stops the reaction.</p> Signup and view all the answers

Which types of metabolic pathways produce sugars from noncarbohydrate molecules?

<p>Gluconeogenesis</p> Signup and view all the answers

Study Notes

Metabolism

  • Defined as the sum of chemical reactions within a cell to obtain/utilize energy, synthesize cellular components, and break down compounds for building blocks.
  • Requires precursor molecules and energy sources for electron flow.

Anabolism and Catabolism

  • Anabolism involves synthesis processes within the cell.
  • Catabolism refers to the breakdown of compounds to harvest energy and building blocks.

Electron Transport and Carriers

  • Oxidation-Reduction cycles involve the movement of electrons, often carried by hydrogen (H-).
  • Key electron carriers include NAD (Nicotinamide Adenine Dinucleotide), NADP (Nicotinamide Adenine Dinucleotide Phosphate), and FAD (Flavin Adenine Dinucleotide).

ATP Functionality

  • ATP serves as the primary energy currency of the cell, fueling synthesis, active transport, and cellular movement.

Enzyme Structure and Function

  • Enzymes can exist as standalone or as part of holoenzymes, which consist of an apoenzyme and cofactors.
  • Enzymatic activity depends on structural integrity, influenced by pH and temperature.

Enzyme Inhibition

  • Competitive Inhibition involves the inhibitor binding to the active site, impacting substrate saturation without denaturing the enzyme.
  • Noncompetitive Inhibition occurs when allosteric sites are occupied, altering enzyme activity without interfering with the active site.
  • Feedback Inhibition involves end products returning to inhibit upstream reactions.

Fermentation and Respiration

  • Carbohydrate catabolism processes include aerobic respiration—which fully converts glucose to CO2 and H2O—and fermentation, which produces lactic acid or ethanol with lesser ATP yield.
  • Different fermentation products include lactic acid (yogurt), ethanol (beer, wine), and carbon dioxide (Swiss cheese).

Cellular Respiration Pathways

  • Key metabolic pathways include glycolysis, the Krebs cycle, and the electron transport chain.
  • NADPH plays a crucial role in synthesizing nucleotides, steroids, and fatty acids.

Amino Acid and Nucleotide Biosynthesis

  • Amino acids are synthesized from precursor molecules derived from glycolysis, the Krebs cycle, and the Pentose Phosphate Pathway.
  • Nucleotides consist of a 5C sugar, a phosphate group, and a nitrogenous base produced from these pathways.

Regulation of Metabolism

  • Cellular metabolism is regulated based on substrate availability and gene expression.
  • Both enzyme control and cellular transport mechanisms are essential in metabolic regulation, with transport proteins modulating substrate concentrations.

Basic Precursor Metabolites

  • Twelve precursor metabolites serve as building blocks for macromolecules and are derived from glycolysis and the Krebs cycle.
  • Specific metabolites are linked to sugar, fatty acids, and amino acid synthesis.

Lipid and Protein Catabolism

  • Lipid catabolism occurs through hydrolysis and beta oxidation, while protein catabolism is utilized when glucose and fats are depleted, relying on amino acid breakdown.

Amphibolic Pathways

  • Amphibolic reactions are capable of functioning in both catabolic and anabolic directions, providing metabolic flexibility (e.g., lipids).

Importance of Fermentation Products

  • Fermentation products serve practical purposes in food production (cheese, yogurt) and can be used to identify microbial species based on the specific products they generate.

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Test your understanding of key concepts in metabolism through these flashcards based on Chapter 5 of Microbiology. This quiz covers essential terms such as metabolism, anabolism, and catabolism, providing definitions and explanations for better comprehension.

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