Podcast
Questions and Answers
What is the purpose of the coarse adjustment knob on a compound microscope?
What is the purpose of the coarse adjustment knob on a compound microscope?
- To adjust the fine focus
- To change the magnification levels
- To improve focus at low powers (correct)
- To adjust the light source
Advanced compound microscopes do not require any external light source.
Advanced compound microscopes do not require any external light source.
True (A)
What are the three types of magnification available on a microscope?
What are the three types of magnification available on a microscope?
Scanning, Low, and High
The total magnification of a microscope is calculated by multiplying the ocular magnification by the __________ magnification.
The total magnification of a microscope is calculated by multiplying the ocular magnification by the __________ magnification.
Match the following microscope types with their characteristics:
Match the following microscope types with their characteristics:
What is one of the major roles of microbes in waste management?
What is one of the major roles of microbes in waste management?
Non-pathogenic microbes can be harmful.
Non-pathogenic microbes can be harmful.
What type of gas is primarily produced by certain microbes during their metabolic processes?
What type of gas is primarily produced by certain microbes during their metabolic processes?
Microorganisms can assist in the manufacturing of _____ used in food products.
Microorganisms can assist in the manufacturing of _____ used in food products.
Match the following microorganisms with their respective roles:
Match the following microorganisms with their respective roles:
Which process is NOT associated with microbes in food manufacturing?
Which process is NOT associated with microbes in food manufacturing?
Microbes can contribute to bioremediation efforts.
Microbes can contribute to bioremediation efforts.
Name one beneficial interaction between microbial communities.
Name one beneficial interaction between microbial communities.
What type of organism produces organic compounds primarily?
What type of organism produces organic compounds primarily?
Molds and mushrooms are unicellular organisms.
Molds and mushrooms are unicellular organisms.
What is the total magnification formula used in microscopes?
What is the total magnification formula used in microscopes?
The lens ability to distinguish two points is known as the ______.
The lens ability to distinguish two points is known as the ______.
Which type of microscope uses immersion oil to reduce light refraction?
Which type of microscope uses immersion oil to reduce light refraction?
Match the following types of microscopy with their descriptions:
Match the following types of microscopy with their descriptions:
A light microscope uses only one type of lens to magnify objects.
A light microscope uses only one type of lens to magnify objects.
What is the refractive index?
What is the refractive index?
Which type of microscope uses electrons to scan the surface of specimens?
Which type of microscope uses electrons to scan the surface of specimens?
The eyepiece of a microscope is fixed and cannot be changed for different magnifications.
The eyepiece of a microscope is fixed and cannot be changed for different magnifications.
What type of specimens might be used with a Transmission Electron Microscope?
What type of specimens might be used with a Transmission Electron Microscope?
The total magnification of a microscope is determined by multiplying the magnification of the eyepiece and the ________.
The total magnification of a microscope is determined by multiplying the magnification of the eyepiece and the ________.
Match the parts of a microscope with their functions:
Match the parts of a microscope with their functions:
What common magnification does the eyepiece of a microscope have?
What common magnification does the eyepiece of a microscope have?
Stage clips are used to hold the slide in place on the stage.
Stage clips are used to hold the slide in place on the stage.
What is the purpose of the diaphragm in a microscope?
What is the purpose of the diaphragm in a microscope?
What is the total magnification when using a 10x eyepiece and a 40x objective lens?
What is the total magnification when using a 10x eyepiece and a 40x objective lens?
Francesco Redi demonstrated that spontaneous generation occurs.
Francesco Redi demonstrated that spontaneous generation occurs.
Who was the first person to observe microorganisms?
Who was the first person to observe microorganisms?
Robert Hooke published a book called ______, which featured drawings of biological materials.
Robert Hooke published a book called ______, which featured drawings of biological materials.
Match the following individuals with their contributions:
Match the following individuals with their contributions:
Which magnification corresponds to the Low Power field of a microscope?
Which magnification corresponds to the Low Power field of a microscope?
Scanning magnification is 10x.
Scanning magnification is 10x.
In what year did Hans and Zacharias Janssen create the first compound microscope?
In what year did Hans and Zacharias Janssen create the first compound microscope?
Who constructed the first electron microscope?
Who constructed the first electron microscope?
Louis Pasteur's experiments with swan-necked flasks proved that microorganisms can arise by spontaneous generation.
Louis Pasteur's experiments with swan-necked flasks proved that microorganisms can arise by spontaneous generation.
What significant development did Carl Zeiss and Ernst Abbe contribute to microscopy?
What significant development did Carl Zeiss and Ernst Abbe contribute to microscopy?
Staining that uses more than one stain to distinguish different features of microorganisms is called __________.
Staining that uses more than one stain to distinguish different features of microorganisms is called __________.
Match the types of stains with their descriptions:
Match the types of stains with their descriptions:
What is a smear in microbiology?
What is a smear in microbiology?
Gram-negative bacteria are more susceptible to penicillin compared to Gram-positive bacteria.
Gram-negative bacteria are more susceptible to penicillin compared to Gram-positive bacteria.
What is the purpose of a mordant in staining?
What is the purpose of a mordant in staining?
The cationic chromophore in a basic dye is __________ charged.
The cationic chromophore in a basic dye is __________ charged.
Which process is used to fix microbes to a slide?
Which process is used to fix microbes to a slide?
Flashcards
Waste Decomposers
Waste Decomposers
Organisms that can break down organic waste effectively and without producing unpleasant smells.
Pathogenic Microbes
Pathogenic Microbes
Microbes that cause diseases.
Biofilm
Biofilm
A community of microbes living in a specific area, often attached to a surface.
Methane Production
Methane Production
A form of energy production that involves breaking down organic matter to produce methane gas.
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Bioremediation
Bioremediation
The process of using microbes to clean up pollution.
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Scientific Names
Scientific Names
A scientific naming system used for organisms, where the genus is capitalized and the specific epithet is lowercase.
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Fermentation in Food
Fermentation in Food
The process of using microbes to produce fermented foods like yogurt, cheese, and bread.
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Photosynthesis in Microbes
Photosynthesis in Microbes
The ability of microbes to perform photosynthesis and fix carbon dioxide for their growth and energy production.
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Amoeba
Amoeba
A single-celled eukaryotic organism that produces its own food through the breakdown of organic molecules like sugars.
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Fungi
Fungi
A group of organisms that includes molds, mushrooms and yeasts. They are eukaryotic and have chitin cell walls. They obtain their energy through the breakdown of organic materials.
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Light Microscope
Light Microscope
A type of microscope that uses light to magnify objects. It uses a combination of lenses to magnify the image.
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Resolution
Resolution
The ability of a lens to distinguish between two closely spaced objects. This is measured in nanometers.
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Refractive Index
Refractive Index
The bending of light as it passes from one medium to another. This is a key property that affects how light interacts with objects.
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Immersion oil
Immersion oil
A type of light microscope that uses a special oil between the objective lens and the specimen to reduce light bending and improve image clarity.
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Compound Light Microscope
Compound Light Microscope
A simple light microscope that uses two lenses to magnify objects. The objective lens magnifies the object, and the ocular lens magnifies the image created by the objective lens.
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Total Magnification
Total Magnification
The total magnification of a compound light microscope is calculated by multiplying the magnification of the objective lens by the magnification of the ocular lens.
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Illuminator
Illuminator
A specialized light source used in some advanced compound microscopes.
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Fine Adjustment Knob
Fine Adjustment Knob
A knob used for fine adjustments to focus the microscope lenses, often used with higher magnification.
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Coarse Adjustment Knob
Coarse Adjustment Knob
A knob used for larger adjustments to focus the microscope lenses, usually for lower magnification.
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Ocular Lens
Ocular Lens
The lens you look through on a microscope, typically has a fixed magnification.
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Scanning Electron Microscope (SEM)
Scanning Electron Microscope (SEM)
A microscope that uses electrons to magnify objects up to two million times. It scans the surface of the specimen.
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Transmission Electron Microscope (TEM)
Transmission Electron Microscope (TEM)
A microscope that uses electrons to magnify objects. It passes electrons through a very thin specimen, revealing its internal structure.
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Eyepiece
Eyepiece
The part of a microscope that you look through to view the specimen. It typically has a magnification of 10x.
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Objective Lenses
Objective Lenses
The lenses located near the specimen that magnify it. Different objectives provide different magnifications (4x, 10x, 40x, etc.).
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Stage
Stage
The platform where you place the specimen to be viewed. It often has a hole to allow light to pass through the specimen.
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Stage Clips
Stage Clips
Two clips on the stage that hold the slide in place while you view the specimen.
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Diaphragm
Diaphragm
A device located beneath the stage, used to adjust the amount of light that passes through the specimen. This helps control the brightness and contrast of view.
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Specimen
Specimen
The stained, prepared specimen being viewed under the microscope. It can be a thin slice, a smear, or a cultured sample.
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Compound Microscope
Compound Microscope
A type of microscopy that uses two lenses: an objective lens and an eyepiece, each magnifying the image.
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Francesco Redi
Francesco Redi
A 17th-century Italian physician who refuted the idea of spontaneous generation by demonstrating that flies breed maggots, not that meat magically produces them.
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Spontaneous Generation
Spontaneous Generation
The idea that living organisms can arise from non-living matter, which was disproven by experiments like Redi's.
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Cell Theory
Cell Theory
A theory that states that all living organisms are composed of one or more cells, and the cell is the fundamental unit of life.
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Anton van Leeuwenhoek
Anton van Leeuwenhoek
A Dutch lens grinder who, in the 17th century, is credited with the first observations of microorganisms using improved microscopes.
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Micrographia
Micrographia
A scientific publication by Robert Hooke in 1660, containing detailed drawings and observations of biological materials made with advanced microscopy.
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Who was Louis Pasteur?
Who was Louis Pasteur?
A microbiologist known for his experiments (swan-necked flasks) that disproved spontaneous generation.
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What is the difference between a simple microscope and a compound microscope?
What is the difference between a simple microscope and a compound microscope?
A simple microscope uses only one lens, while a compound microscope uses two or more lenses to magnify the object.
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What is staining?
What is staining?
Using a dye to color a microbe and emphasize its structure.
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What is a smear?
What is a smear?
A smear is a thin film of a microbe solution on a slide, fixed to attach and kill microbes.
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What is a simple stain?
What is a simple stain?
This type of staining uses only one dye. It helps visualize the basic morphology (shape) of a microbe.
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What is a differential stain?
What is a differential stain?
This staining technique uses multiple dyes to distinguish between different types of microbes.
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What is the Gram stain?
What is the Gram stain?
The Gram stain is a differential staining technique used to classify bacteria into Gram-positive and Gram-negative.
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What is a special stain?
What is a special stain?
Used to distinguish special parts of microbes, such as capsules, endospores, and flagella.
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What is a basic dye?
What is a basic dye?
A basic dye has a positively charged chromophore that binds to negatively charged components of the microbe, staining the microbe itself.
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What is an acidic dye?
What is an acidic dye?
An acidic dye has a negatively charged chromophore that is repelled by the microbe, staining the background.
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Microbiology BIOL333
- Course Information:
- Professor: Dr. Rana El Hajj
- Email: r.hajj@bau.edu.lb
- Attendance is mandatory
- Assessment 1 (Midterm): 30% of final grade
- Assessment 2 (Presentations): 20% of final grade
Topics in the Assignment
- Bacterial Interactions: An application on bacterial interactions in the environment; including quorum sensing.
- Pathogenic Bacteria: An application on a pathogenic bacterium in the human body; and its virulence factors.
- Hand Sanitizers: How do they kill microbes? Are they safe?
- Pasteurization: Pasteurization of milk, types of bacteria killed, types remaining.
- Biofilms: Biofilms in Hospitals, examples, and effects on health.
- Cholera: Cholera updates
- Bioremediation: Principle and an application about it.
Introduction to the Microbial World
- Classification: Organisms were previously classified into animal or plant kingdoms.
- Woese's System: Carl Woese developed a new classification system based on molecular and cellular characteristics.
- Domains: Three domains:
- Eubacteria: Bacteria with peptidoglycan cell walls
- Archaea: Prokaryotes without peptidoglycan cell walls
- Eucarya: Organism in the kingdoms: Protista, Fungi, Plantae, and Animalia
What is Microbiology?
- Definition: The study of microorganisms (usually less than 1 mm in diameter) and their activities.
- Excluded Entities: Viruses, viroids, and prions are not considered organisms.
- Prokaryotes: Bacteria and archaea
- Eukaryotes: Algae, fungi, and protozoa
Microbiology Themes
- Basic Microbiology: Disease-related (etiology, epidemiology, infection control), environmentally-related and industrially-related
- Applied Microbiology: By organism, by process (microbes and their roles)
Measurement Units
- Units of Measurement: Includes nanometers (nm), micrometers (µm), millimeters (mm).
Comparison of Domains
- Bacteria, Archaea, and Eucarya: Comparison based on size, nuclear membrane, cell wall composition, and cytoplasmic structures (mitochondria, chloroplasts, and cytoskeleton).
Eukaryotic Members
- Algae, Fungi and Protozoa: A comparison of the various types within these groupings based on cell organization, energy source, and size; a breakdown of species within the groups.
History of Microbiology
- Early Discoveries: Robert Hooke described fungi and protozoa. Antony van Leeuwenhoek observed bacteria and yeast, and created powerful microscopes with limited resolution.
- Spontaneous Generation: Italian naturalist Spallanzani provided evidence that life does not spontaneously arise.
- Germ Theory: Pasteur provided strong arguments with his 'swan-necked flask' experiments.
- Koch's Postulates: A set of criteria to link a microbe to a specific disease.
- Other discoveries: The discovery of viruses, antibiotics and the development of other laboratory techniques and procedures.
Microbiology and the Lab
- Koch's Postulates: Steps for identifying diseases and their causes (description and detailed diagram)
- Microbial Laboratory Techniques: Techniques for staining, culturing, and working with pure cultures, and the importance of aseptic techniques.
- Microbial Growth Media: Use of culture media to grow microbes in the lab.
Microorganisms and Food
- Food Preservation: How microorganisms can be used to preserve foods (e.g., tempeh).
- Food Manufacture: How microorganisms assist in food manufacturing (e.g., cheese, bread.)
- Energy: How microorganisms are involved in energy production (e.g., methane production).
- Environmental Applications: Use in water treatment and waste management.
Microbes and Disease
- Microbial Diseases: Overview of how some microbes cause diseases.
Naming and Classifying Microorganisms
- Scientific Names: The binomial system of nomenclature (e.g., Escherichia coli).
- Classification Levels: Hierarchical levels from domain to species, focusing on microbial types.
Types of Eukaryotes
- Protozoa: Unicellular organisms, gaining energy through absorption or ingestion of organic chemicals. They can move using pseudopods, cilia, or flagella.
- Algae: Unicellular or multicellular organisms, performing photosynthesis, and have cellulose cell walls
- Fungi: Eukaryotic, obtaining energy through organic chemicals; molds and mushrooms are multicellular.
- Multicellular Animal parasites: Like flatworms and roundworms.
Microscopy
- Compound Light Microscope: Magnification techniques, resolution, contrast, refractive index, and immersion oil use.
- Types of Microscopes: Light, stereoscopic, SEM, and TEM microscopes; advantages and disadvantages of each
- Parts of a Microscope: Detailed descriptions of different parts, their functions, and how they work together.
- Types of Stains: Gram staining, Acid-fast stain, and differential stains - descriptions, procedures and uses.
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