Biology Chapter 1: Organisms and Diseases
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Questions and Answers

Match the following microbial types with their cellular characteristics:

Bacteria = Prokaryotic, unicellular Archaea = Prokaryotic, unicellular Fungi = Eukaryotic, unicellular or multicellular Viruses = Non-cellular, protein + nucleic acid

Match the following symbiotic relationships with their descriptions:

Mutualism = Host and Microbe benefit Commensalism = Host unaffected, Microbe benefits Parasitism = Host hurt, Microbe benefits Opportunistic Pathogens = Prey on weak host

Match the following terms with their definitions:

Endemic diseases = Occurring in a specific community Sporadic diseases = Occurring without concentration Pandemics = Internationally occurring Emerging diseases = New or re-populating disease

Match the following scientists with their contributions to microbiology:

<p>Leeuwenhoek = Microscope Pasteur = Swan neck flasks, disproved abiogenesis Koch = Four postulates of disease Jenner = Smallpox vaccination</p> Signup and view all the answers

Match the following bond types with their descriptions:

<p>Covalent bonds = Sharing of electrons Ionic bonds = Losing or gaining electrons Hydrogen bonds = Weak polar bond between hydrogen and a molecule Nonpolar covalent bonds = Equal sharing of electrons</p> Signup and view all the answers

Match the following chemical reactions with their descriptions:

<p>Synthesis = A + B = AB Decomposition = AB = A + B Exchange = AB + CD = AD + CB Hydrolysis = Breaking down bonds with water</p> Signup and view all the answers

Match the following functional groups with their characteristics:

<p>Hydroxyl = OH, often found in carbohydrates Carboxyl = Found in fatty acids, proteins, and organic acids Amino = Found in proteins and nucleic acids Phosphate = Found in DNA, RNA, and ATP</p> Signup and view all the answers

Match the following macromolecules with their building blocks:

<p>Carbohydrates = Monosaccharides Lipids = Glycerol and fatty acids Proteins = Amino acids Nucleic acids = Nucleotides</p> Signup and view all the answers

Match the following types of carbohydrates with their examples:

<p>Monosaccharides = Glucose, galactose, fructose Disaccharides = Maltose, lactose, sucrose Polysaccharides = Cellulose, glycogen, starch</p> Signup and view all the answers

Match the following terms related to bacterial structure with their corresponding descriptions:

<p>Cell wall = Provides structural support and protection, composed of peptidoglycan in most bacteria Plasma membrane = A phospholipid bilayer that regulates the passage of molecules into and out of the cell Nucleoid = The region within the cytoplasm where the bacterial chromosome is located Plasmids = Small, circular DNA molecules that replicate independently of the bacterial chromosome</p> Signup and view all the answers

Match the following types of lipids with their characteristics:

<p>Triglycerides = 1 glycerol + 3 fatty acids Fatty acids = Saturated (single bonds) or unsaturated (double bonds) Phospholipids = 1 glycerol + 2 fatty acids + phosphate group Steroids = Cholesterol</p> Signup and view all the answers

Match the following types of bacteria with their characteristic shapes:

<p>Coccus = Sphere-shaped Bacillus = Rod-shaped Spirilla = Spiral-shaped Vibrio = Comma-shaped</p> Signup and view all the answers

Match the following terms related to eukaryotic cell structures with their corresponding functions:

<p>Nucleus = Contains the cell's genetic material (DNA) and controls cellular activities Endoplasmic reticulum (ER) = A network of membranes involved in protein synthesis, lipid metabolism, and other processes Golgi apparatus = Modifies and packages proteins and lipids for secretion or delivery to other organelles Lysosomes = Organelles responsible for intracellular digestion</p> Signup and view all the answers

Match the following types of staining methods with their applications:

<p>Simple stain = Used to visualize the shape, size, and arrangement of bacteria Negative stain = Used to stain the background, highlighting the bacteria's shape and capsule Acid-fast stain = Used to identify bacteria with a waxy cell wall, such as Mycobacteria Structural stain = Used to visualize specific structures, such as capsules or flagella</p> Signup and view all the answers

Match the following types of media with their characteristics:

<p>Liquid media = Contains no agar and allows for the growth of bacteria in a liquid environment Semi-solid media = Contains a low concentration of agar, creating a semi-solid consistency for studying bacterial motility General media = Supports the growth of a wide range of bacteria Enriched media = Contains additional nutrients to support the growth of fastidious bacteria</p> Signup and view all the answers

Match the following terms related to eukaryotic cell processes with their definitions:

<p>Endocytosis = The process by which cells take in material from their surroundings Phagocytosis = The engulfment of solid particles by cells Pinocytosis = The uptake of fluid by cells Exocytosis = The process by which cells release material from their interior</p> Signup and view all the answers

Match the following terms related to bacterial cell walls with their descriptions:

<p>Gram-negative bacteria = Have a thin layer of peptidoglycan and an outer membrane Gram-positive bacteria = Have a thick layer of peptidoglycan and lack an outer membrane Mycobacteria = Have a cell wall containing peptidoglycan and mycolic acids Mycoplasmas = Lack a cell wall and have a plasma membrane that is resistant to lysis</p> Signup and view all the answers

Match the following types of cellular structures with their primary function:

<p>Cilia = Short, hair-like projections that aid in feeding or locomotion Flagella = Long, whip-like structures that propel bacteria Endospores = Resistant structures formed by some bacteria to survive harsh conditions Ribosomes = Organelles responsible for protein synthesis</p> Signup and view all the answers

Flashcards

Prokaryotes

Unicellular organisms without a nucleus, such as bacteria and archaea.

Eukaryotes

Organisms with a nucleus, can be unicellular or multicellular, like protists and fungi.

Mutualism

A type of symbiosis where both host and microbe benefit.

Commensalism

A symbiotic relationship where the microbe benefits and the host is unaffected.

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Parasitism

A relationship where the microbe benefits at the host's expense, causing harm.

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Viruses

Non-cellular entities made of protein and nucleic acid that can infect living cells.

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Prions

Infectious agents composed entirely of protein, can cause disease.

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Koch’s Postulates

Set of criteria to establish a microbe as the cause of a disease.

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Abiogenesis

The theory that life can arise from non-living matter.

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Acids

Substances that release hydrogen ions (H+) in a solution, pH less than 7.

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Covalent Bonds

Chemical bonds formed by the sharing of electrons between atoms.

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Lipids

Fatty substances that include triglycerides, phospholipids, and steroids.

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Cell Wall

A protective layer that maintains cell shape and prevents lysis in a hypotonic solution.

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Flagella

Tail-like structures that provide motility to bacteria.

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Biofilms

Communities of microorganisms embedded in a matrix, can be harmful like dental plaque.

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Penicillin sensitivity

The susceptibility of bacteria to penicillin due to cell wall structure.

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Plasmids

Small circular DNA that replicate independently of chromosomes in bacteria.

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Cytoskeleton

Network providing shape and support in cells, made of actin filaments.

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Endocytosis

The process of a cell taking in materials by engulfing them in a membrane.

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Mitochondria

The organelle responsible for ATP production and energy metabolism in cells.

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Negative stain

A staining technique that colors the background but leaves the specimen clear.

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Rough ER

Endoplasmic reticulum studded with ribosomes, involved in protein synthesis.

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Selective media

Growth medium that allows only certain types of bacteria to grow.

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Study Notes

Chapter 1: Cellular and Non-Cellular Organisms

  • Prokaryotes: Bacteria and Archaea; unicellular; no nucleus.
  • Eukaryotes: Protists, Fungi, Helminths; unicellular or multicellular; have a nucleus and organelles.
  • Viruses: Non-cellular; protein + nucleic acid.
  • Prions: Non-cellular; protein only.
  • Reproduction: Prokaryotes reproduce asexually (binary fission); Eukaryotes reproduce asexually (mitosis) or sexually (meiosis).

Chapter 1: Microbial Interactions & Disease

  • Symbiotic Relationships:
    • Mutualism: Both host and microbe benefit.
    • Commensalism: Host unaffected; microbe benefits.
    • Parasitism: Host harmed; microbe benefits.
  • Types of Pathogens:
    • Normal Pathogens: Present on the skin.
    • Transient Pathogens: Come and go.
    • Opportunistic Pathogens: Attack weakened hosts.
    • True Pathogens: Always cause disease in a healthy host.
    • Emerging Pathogens: New or re-emerging diseases.
    • Re-emerging Pathogens: Formerly controlled diseases re-emerging.
  • Disease Patterns: Endemic, sporadic, pandemic

Chapter 1: Historical Figures and Concepts

  • Biogenesis: Life comes from life.
  • Abiogenesis: Life comes from non-living things.
  • Leeuwenhoek: Invented the microscope.
  • Pasteur: Demonstrated that dust in the air is a source of microorganisms via swan-neck flask experiment.
  • Koch's Postulates: Criteria to establish a specific microbe as the cause of a particular disease.
  • Redi: Experiments with meat in jars to validate biogenesis against spontaneous generation.
  • Lister: Pioneered sterilization techniques.
  • Jenner: Developed smallpox vaccination.
  • Semmelweis: Introduced aseptic techniques.
  • Needham: Results supported abiogenesis.

Chapter 1 : Key Terms

  • Etiology: The cause of a disease (intrinsic, extrinsic, idiopathic)
  • Taxonomic Categories: Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species (e.g., Genus species)

Chapter 2: Atoms, Molecules, and Bonds

  • Atoms: Simplest form of an element, indivisible.
  • Elements: Made of atoms; 95% of body weight: C, H, O, N, S, P
  • Subatomic Particles: Proton (positive), neutron (neutral), electron (negative).
  • Atomic Number: Number of protons.
  • Mass Number: Number of protons + neutrons.
  • Isotopes: Forms of an element with different numbers of neutrons.
  • Valence Electrons: Electrons in the outermost shell; determine bonding behavior.
  • Molecule vs. Compound: Molecules (covalent bonds) form compounds (covalent or ionic bonds).
  • Covalent Bonds: Sharing of electrons.
  • Ionic Bonds: Transfer of electrons creating ions (cations + / anions -).
  • Hydrogen Bonds: Weak polar bonds between hydrogen and electronegative atoms.
  • Chemical Reactions: Synthesis (A+B=AB), Decomposition (AB=A+B), Exchange Reactions (AB+CD=AD+CB).
  • Solutions: Solute + Solvent
  • pH Scale: Measures hydrogen ion concentration; scale of 0-14. Acids (pH < 7); Bases (pH > 7).
  • Organic Molecules: Molecules containing carbon and hydrogen.
  • Carbon's Importance: 4 valence electrons, forms single, double and triple bonds.
  • Functional Groups: Groups of atoms that impart specific chemical properties, found in organic molecules.

Chapter 2: Macromolecules

  • Macromolecules: Large polymers built from monomers.
  • Carbohydrates: Monosaccharides (glucose, fructose, galactose) → Disaccharides (maltose, sucrose, lactose), Polysaccharides (starch, glycogen, cellulose).
  • Lipids: Fatty substances (triglycerides, fatty acids, phospholipids, steroids).
  • Proteins: Chains of amino acids linked by peptide bonds.
  • Nucleic Acids: DNA and RNA; composed of nucleotides.

Chapter 3: Microbial Morphology

  • Structures for Attachment and Motility:

    • Flagella: Mobility; monotrichous, lophotrichous, amphitrichous, peritrichous, periplasmic.
    • Fimbriae: Short, hair-like structures for attachment.
    • Pili: Long, hollow appendages for conjugation.
  • External Appendages:

    • Glycocalyx: General term for sticky outer layer
  • Slime Layer: Loose glycocalyx protecting from desiccation and nutrient loss.

  • Capsule: Polysaccharides and/or proteins bound firmly; protects from phagocytosis via immune cells.

  • Biofilms: Groups of microorganisms encased in a polysaccharide matrix

  • Cell Wall: Rigid structure for shape and protection, prevents lysis.

    • Peptidoglycan: Present in bacteria; composed of alternating NAG and NAM subunits.
    • Gram-Positive: Thick peptidoglycan layer. Susceptible to penicillin; contains teichoic acid; lipoteichoic acid.
    • Gram-Negative: Thin peptidoglycan layer surrounded by an outer membrane.
    • Mycobacteria: Has mycolic acid and PG
  • Cell Wall Variations:

    • Mycoplasmas: Lack a cell wall.
    • L-forms: Loss of cell wall due to mutation or treatment.
  • Protoplasts /Spheroplasts: Cells that have lost their cell wall.

  • Plasma Membrane: Phospholipid bilayer; regulates permeability.

  • Intracellular Structures:

    • Nucleoid: Region where the DNA is located.
    • Plasmids: Small circles of DNA replicating independently of the chromosome.
    • Ribosomes: Site of protein synthesis.
    • Cytoskeleton: Provides structure; made of protein filaments.
    • Endospores: Dormant (protective) structures formed by some bacteria.
  • Bacterial Shapes: Coccus (spherical), Bacillus (rod-shaped), Spirillum (spiral), Spirochete (corkscrew).

  • Arrangement/ Grouping of Bacteria:

  • Streptococcus: Sphere-shaped bacteria arranged in chains.

  • Staphylococcus: Sphere-shaped bacteria arranged in clusters.

Chapter 4: Eukaryotic Cell Structure

  • General Features: All eukaryotic cells contain membrane-bound organelles.
  • Eukaryotic Cell Membrane: Phospholipid bilayer with sterols and proteins.
  • Endomembrane system: ER, Golgi, lysosomes
  • Endocytosis vs Exocytosis:
  • Endocytosis: Uptake of materials by vesicles.
  • Phagocytosis: Engulfing large particles.
  • Pinocytosis: Uptake of fluids
  • Exocytosis: Secretion of materials in vesicles.
  • Constitutive vs regulated: Controlled secretion and non-controlled secretion
  • Nucleus: Contains chromosomes, directs cell activity.
  • Nucleolus: Site of ribosome synthesis within nucleus.
  • Endoplasmic Reticulum (ER): Transport and synthesis of materials.
    • Rough ER: Protein synthesis.
    • Smooth ER: Lipid synthesis, metabolism, detoxification.
  • Golgi Apparatus: Modifies and packages proteins.
  • Lysosomes: Contain digestive enzymes.
  • Mitochondria: Site of cellular respiration, ATP production
  • Chloroplasts: Site of photosynthesis
  • Ribosomes: Composed of 40S + 60S subunits.
  • Cytoskeleton: Provides structure and movement.

Chapter 5: Staining and Culture Methods

  • Staining: Techniques using dyes to visualize cells.
  • Positive Stain: Dyes bind to cell components.
  • Negative Stain: Dyes do not enter the cell; highlight background; cell outline is visible.
  • Acid-Fast Stain: Differentiates mycobacteria.
  • Specialized Stain: to identify special structures (flagella, capsule)
  • Types of Media: Liquid, semi-solid, solid
    • Simple media: general support for many bacteria
    • Enriched media: Supplements for increased growth
    • Reducing Media: Anaerobic growth
    • Selective media: Grow desired bacteria.
    • Differential Media: Distinguish microorganisms based on biochemical reactions (e.g. color change)

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Explore the fundamental concepts of cellular and non-cellular organisms, including prokaryotes and eukaryotes. Understand microbial interactions and various types of pathogens. This quiz will test your knowledge on single-celled life forms and their roles in disease.

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