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Questions and Answers
Match the following microbial types with their cellular characteristics:
Match the following microbial types with their cellular characteristics:
Bacteria = Prokaryotic, unicellular Archaea = Prokaryotic, unicellular Fungi = Eukaryotic, unicellular or multicellular Viruses = Non-cellular, protein + nucleic acid
Match the following symbiotic relationships with their descriptions:
Match the following symbiotic relationships with their descriptions:
Mutualism = Host and Microbe benefit Commensalism = Host unaffected, Microbe benefits Parasitism = Host hurt, Microbe benefits Opportunistic Pathogens = Prey on weak host
Match the following terms with their definitions:
Match the following terms with their definitions:
Endemic diseases = Occurring in a specific community Sporadic diseases = Occurring without concentration Pandemics = Internationally occurring Emerging diseases = New or re-populating disease
Match the following scientists with their contributions to microbiology:
Match the following scientists with their contributions to microbiology:
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Match the following bond types with their descriptions:
Match the following bond types with their descriptions:
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Match the following chemical reactions with their descriptions:
Match the following chemical reactions with their descriptions:
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Match the following functional groups with their characteristics:
Match the following functional groups with their characteristics:
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Match the following macromolecules with their building blocks:
Match the following macromolecules with their building blocks:
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Match the following types of carbohydrates with their examples:
Match the following types of carbohydrates with their examples:
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Match the following terms related to bacterial structure with their corresponding descriptions:
Match the following terms related to bacterial structure with their corresponding descriptions:
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Match the following types of lipids with their characteristics:
Match the following types of lipids with their characteristics:
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Match the following types of bacteria with their characteristic shapes:
Match the following types of bacteria with their characteristic shapes:
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Match the following terms related to eukaryotic cell structures with their corresponding functions:
Match the following terms related to eukaryotic cell structures with their corresponding functions:
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Match the following types of staining methods with their applications:
Match the following types of staining methods with their applications:
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Match the following types of media with their characteristics:
Match the following types of media with their characteristics:
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Match the following terms related to eukaryotic cell processes with their definitions:
Match the following terms related to eukaryotic cell processes with their definitions:
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Match the following terms related to bacterial cell walls with their descriptions:
Match the following terms related to bacterial cell walls with their descriptions:
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Match the following types of cellular structures with their primary function:
Match the following types of cellular structures with their primary function:
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Flashcards
Prokaryotes
Prokaryotes
Unicellular organisms without a nucleus, such as bacteria and archaea.
Eukaryotes
Eukaryotes
Organisms with a nucleus, can be unicellular or multicellular, like protists and fungi.
Mutualism
Mutualism
A type of symbiosis where both host and microbe benefit.
Commensalism
Commensalism
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Parasitism
Parasitism
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Viruses
Viruses
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Prions
Prions
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Koch’s Postulates
Koch’s Postulates
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Abiogenesis
Abiogenesis
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Acids
Acids
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Covalent Bonds
Covalent Bonds
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Lipids
Lipids
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Cell Wall
Cell Wall
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Flagella
Flagella
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Biofilms
Biofilms
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Penicillin sensitivity
Penicillin sensitivity
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Plasmids
Plasmids
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Cytoskeleton
Cytoskeleton
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Endocytosis
Endocytosis
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Mitochondria
Mitochondria
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Negative stain
Negative stain
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Rough ER
Rough ER
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Selective media
Selective media
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Study Notes
Chapter 1: Cellular and Non-Cellular Organisms
- Prokaryotes: Bacteria and Archaea; unicellular; no nucleus.
- Eukaryotes: Protists, Fungi, Helminths; unicellular or multicellular; have a nucleus and organelles.
- Viruses: Non-cellular; protein + nucleic acid.
- Prions: Non-cellular; protein only.
- Reproduction: Prokaryotes reproduce asexually (binary fission); Eukaryotes reproduce asexually (mitosis) or sexually (meiosis).
Chapter 1: Microbial Interactions & Disease
- Symbiotic Relationships:
- Mutualism: Both host and microbe benefit.
- Commensalism: Host unaffected; microbe benefits.
- Parasitism: Host harmed; microbe benefits.
- Types of Pathogens:
- Normal Pathogens: Present on the skin.
- Transient Pathogens: Come and go.
- Opportunistic Pathogens: Attack weakened hosts.
- True Pathogens: Always cause disease in a healthy host.
- Emerging Pathogens: New or re-emerging diseases.
- Re-emerging Pathogens: Formerly controlled diseases re-emerging.
- Disease Patterns: Endemic, sporadic, pandemic
Chapter 1: Historical Figures and Concepts
- Biogenesis: Life comes from life.
- Abiogenesis: Life comes from non-living things.
- Leeuwenhoek: Invented the microscope.
- Pasteur: Demonstrated that dust in the air is a source of microorganisms via swan-neck flask experiment.
- Koch's Postulates: Criteria to establish a specific microbe as the cause of a particular disease.
- Redi: Experiments with meat in jars to validate biogenesis against spontaneous generation.
- Lister: Pioneered sterilization techniques.
- Jenner: Developed smallpox vaccination.
- Semmelweis: Introduced aseptic techniques.
- Needham: Results supported abiogenesis.
Chapter 1 : Key Terms
- Etiology: The cause of a disease (intrinsic, extrinsic, idiopathic)
- Taxonomic Categories: Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species (e.g., Genus species)
Chapter 2: Atoms, Molecules, and Bonds
- Atoms: Simplest form of an element, indivisible.
- Elements: Made of atoms; 95% of body weight: C, H, O, N, S, P
- Subatomic Particles: Proton (positive), neutron (neutral), electron (negative).
- Atomic Number: Number of protons.
- Mass Number: Number of protons + neutrons.
- Isotopes: Forms of an element with different numbers of neutrons.
- Valence Electrons: Electrons in the outermost shell; determine bonding behavior.
- Molecule vs. Compound: Molecules (covalent bonds) form compounds (covalent or ionic bonds).
- Covalent Bonds: Sharing of electrons.
- Ionic Bonds: Transfer of electrons creating ions (cations + / anions -).
- Hydrogen Bonds: Weak polar bonds between hydrogen and electronegative atoms.
- Chemical Reactions: Synthesis (A+B=AB), Decomposition (AB=A+B), Exchange Reactions (AB+CD=AD+CB).
- Solutions: Solute + Solvent
- pH Scale: Measures hydrogen ion concentration; scale of 0-14. Acids (pH < 7); Bases (pH > 7).
- Organic Molecules: Molecules containing carbon and hydrogen.
- Carbon's Importance: 4 valence electrons, forms single, double and triple bonds.
- Functional Groups: Groups of atoms that impart specific chemical properties, found in organic molecules.
Chapter 2: Macromolecules
- Macromolecules: Large polymers built from monomers.
- Carbohydrates: Monosaccharides (glucose, fructose, galactose) → Disaccharides (maltose, sucrose, lactose), Polysaccharides (starch, glycogen, cellulose).
- Lipids: Fatty substances (triglycerides, fatty acids, phospholipids, steroids).
- Proteins: Chains of amino acids linked by peptide bonds.
- Nucleic Acids: DNA and RNA; composed of nucleotides.
Chapter 3: Microbial Morphology
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Structures for Attachment and Motility:
- Flagella: Mobility; monotrichous, lophotrichous, amphitrichous, peritrichous, periplasmic.
- Fimbriae: Short, hair-like structures for attachment.
- Pili: Long, hollow appendages for conjugation.
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External Appendages:
- Glycocalyx: General term for sticky outer layer
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Slime Layer: Loose glycocalyx protecting from desiccation and nutrient loss.
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Capsule: Polysaccharides and/or proteins bound firmly; protects from phagocytosis via immune cells.
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Biofilms: Groups of microorganisms encased in a polysaccharide matrix
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Cell Wall: Rigid structure for shape and protection, prevents lysis.
- Peptidoglycan: Present in bacteria; composed of alternating NAG and NAM subunits.
- Gram-Positive: Thick peptidoglycan layer. Susceptible to penicillin; contains teichoic acid; lipoteichoic acid.
- Gram-Negative: Thin peptidoglycan layer surrounded by an outer membrane.
- Mycobacteria: Has mycolic acid and PG
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Cell Wall Variations:
- Mycoplasmas: Lack a cell wall.
- L-forms: Loss of cell wall due to mutation or treatment.
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Protoplasts /Spheroplasts: Cells that have lost their cell wall.
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Plasma Membrane: Phospholipid bilayer; regulates permeability.
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Intracellular Structures:
- Nucleoid: Region where the DNA is located.
- Plasmids: Small circles of DNA replicating independently of the chromosome.
- Ribosomes: Site of protein synthesis.
- Cytoskeleton: Provides structure; made of protein filaments.
- Endospores: Dormant (protective) structures formed by some bacteria.
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Bacterial Shapes: Coccus (spherical), Bacillus (rod-shaped), Spirillum (spiral), Spirochete (corkscrew).
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Arrangement/ Grouping of Bacteria:
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Streptococcus: Sphere-shaped bacteria arranged in chains.
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Staphylococcus: Sphere-shaped bacteria arranged in clusters.
Chapter 4: Eukaryotic Cell Structure
- General Features: All eukaryotic cells contain membrane-bound organelles.
- Eukaryotic Cell Membrane: Phospholipid bilayer with sterols and proteins.
- Endomembrane system: ER, Golgi, lysosomes
- Endocytosis vs Exocytosis:
- Endocytosis: Uptake of materials by vesicles.
- Phagocytosis: Engulfing large particles.
- Pinocytosis: Uptake of fluids
- Exocytosis: Secretion of materials in vesicles.
- Constitutive vs regulated: Controlled secretion and non-controlled secretion
- Nucleus: Contains chromosomes, directs cell activity.
- Nucleolus: Site of ribosome synthesis within nucleus.
- Endoplasmic Reticulum (ER): Transport and synthesis of materials.
- Rough ER: Protein synthesis.
- Smooth ER: Lipid synthesis, metabolism, detoxification.
- Golgi Apparatus: Modifies and packages proteins.
- Lysosomes: Contain digestive enzymes.
- Mitochondria: Site of cellular respiration, ATP production
- Chloroplasts: Site of photosynthesis
- Ribosomes: Composed of 40S + 60S subunits.
- Cytoskeleton: Provides structure and movement.
Chapter 5: Staining and Culture Methods
- Staining: Techniques using dyes to visualize cells.
- Positive Stain: Dyes bind to cell components.
- Negative Stain: Dyes do not enter the cell; highlight background; cell outline is visible.
- Acid-Fast Stain: Differentiates mycobacteria.
- Specialized Stain: to identify special structures (flagella, capsule)
- Types of Media: Liquid, semi-solid, solid
- Simple media: general support for many bacteria
- Enriched media: Supplements for increased growth
- Reducing Media: Anaerobic growth
- Selective media: Grow desired bacteria.
- Differential Media: Distinguish microorganisms based on biochemical reactions (e.g. color change)
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Explore the fundamental concepts of cellular and non-cellular organisms, including prokaryotes and eukaryotes. Understand microbial interactions and various types of pathogens. This quiz will test your knowledge on single-celled life forms and their roles in disease.