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Questions and Answers
What is the purpose of flooding the smear with Gram's Crystal Violet for 1 minute?
What is the purpose of flooding the smear with Gram's Crystal Violet for 1 minute?
- To counterstain with Safranin
- To apply the decolorizer
- To fix the bacterial suspension (correct)
- To wash the slide
Why is it important to air dry or blot dry the slide between sheets of clean paper after counterstaining with Safranin?
Why is it important to air dry or blot dry the slide between sheets of clean paper after counterstaining with Safranin?
- To prevent the bacteria from spreading out on the slide
- To remove excess Safranin dye (correct)
- To enhance the color intensity of the stain
- To fix the Safranin stain
What is the purpose of adding decolorizer (95% ethanol) drop-by-drop to the smear during the Gram staining process?
What is the purpose of adding decolorizer (95% ethanol) drop-by-drop to the smear during the Gram staining process?
- To fix the bacteria to the slide
- To enhance the color of the stain
- To remove excess crystal violet
- To differentiate between Gram-positive and Gram-negative bacteria (correct)
What does spreading the bacterial suspension thin on the slide with a cover slip help achieve?
What does spreading the bacterial suspension thin on the slide with a cover slip help achieve?
Why is it crucial to rinse the smear with distilled water after applying Gram's Iodine?
Why is it crucial to rinse the smear with distilled water after applying Gram's Iodine?
Why should one examine the finished smear under HPO and take a picture as part of the Gram staining process?
Why should one examine the finished smear under HPO and take a picture as part of the Gram staining process?
What is the purpose of staining in microbiology?
What is the purpose of staining in microbiology?
What is the main difference between simple stains and differential stains?
What is the main difference between simple stains and differential stains?
What is the primary stain used in the Gram staining method?
What is the primary stain used in the Gram staining method?
Why does peptidoglycan not dissolve in organic solvents during the Gram staining process?
Why does peptidoglycan not dissolve in organic solvents during the Gram staining process?
Which type of bacteria would appear purple after the Gram staining process?
Which type of bacteria would appear purple after the Gram staining process?
Which reagent in the Gram staining method acts as a mordant?
Which reagent in the Gram staining method acts as a mordant?
What is the purpose of the malachite green staining procedure in microbiology?
What is the purpose of the malachite green staining procedure in microbiology?
Which type of bacteria retains the blue stain in the gram staining procedure?
Which type of bacteria retains the blue stain in the gram staining procedure?
What is the characteristic distinction between gram-positive and gram-negative bacteria?
What is the characteristic distinction between gram-positive and gram-negative bacteria?
Which type of bacteria is characterized by sometimes retaining and sometimes not retaining the blue color in gram staining?
Which type of bacteria is characterized by sometimes retaining and sometimes not retaining the blue color in gram staining?
What is a typical example of a gram-positive bacteria mentioned in the text?
What is a typical example of a gram-positive bacteria mentioned in the text?
What is one of the objectives of the mentioned activity in the text related to microbiology?
What is one of the objectives of the mentioned activity in the text related to microbiology?
Which component of the cell wall is primarily affected by the decolorizer in the Gram staining process?
Which component of the cell wall is primarily affected by the decolorizer in the Gram staining process?
What is the purpose of the counterstain safranin in Gram staining?
What is the purpose of the counterstain safranin in Gram staining?
What color will Gram-positive cells appear after the Gram staining process?
What color will Gram-positive cells appear after the Gram staining process?
What is released by Gram-negative cells when they die and contribute to fever and malaise?
What is released by Gram-negative cells when they die and contribute to fever and malaise?
How would rod-shaped, purple (blue) cells be reported after a Gram stain procedure?
How would rod-shaped, purple (blue) cells be reported after a Gram stain procedure?
What is the staining reaction displayed by cells that appear pink or red after a Gram stain procedure?
What is the staining reaction displayed by cells that appear pink or red after a Gram stain procedure?
What is the purpose of a mordant in the gram staining procedure?
What is the purpose of a mordant in the gram staining procedure?
Why is it important to use a mordant in the gram staining process?
Why is it important to use a mordant in the gram staining process?
Which step of the gram staining process involves the application of a mordant?
Which step of the gram staining process involves the application of a mordant?
Why is counterstain used in the gram staining technique?
Why is counterstain used in the gram staining technique?
Which part of the gram staining process ensures that Gram-positive bacteria retain the crystal violet stain?
Which part of the gram staining process ensures that Gram-positive bacteria retain the crystal violet stain?
In gram staining, what would happen if a mordant was omitted from the process?
In gram staining, what would happen if a mordant was omitted from the process?
