Microbiology and Immunology Quiz

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Questions and Answers

Which type of microscope primarily relies on transmitted light?

  • Electron Microscopy
  • Phase Contrast Microscope
  • Darkfield Microscope
  • Light Microscope (correct)

What is the main characteristic of light used in a light microscope?

  • It is refracted/undeviated light. (correct)
  • It is reflected light.
  • It is illuminated from above the specimen.
  • It can only be used in electron microscopy.

Which of the following microscopes does NOT use transmitted light as its primary illumination method?

  • Phase Contrast Microscope
  • Electron Microscopy (correct)
  • Light Microscope
  • Darkfield Microscope

In a darkfield microscope, how is the light utilized compared to a light microscope?

<p>Reflected light only. (A)</p> Signup and view all the answers

What type of specimen is most effectively viewed under a light microscope?

<p>Transparent specimens that allow light to pass through. (B)</p> Signup and view all the answers

What stage of Giardia lamblia is relevant for human infection?

<p>Cysts (B)</p> Signup and view all the answers

Which larval form is associated with Schistosoma hematobium?

<p>Cercaria larva (A)</p> Signup and view all the answers

Which of the following is the infective form of Leishmania?

<p>Promastigote (A)</p> Signup and view all the answers

Identify the correct stage for Ascaris that is known for causing infection.

<p>Embryonated egg (A)</p> Signup and view all the answers

Which stage is indicative of Toxoplasma's presence in undercooked meat?

<p>Bradyzoites (A)</p> Signup and view all the answers

Which immunoglobulin type has the shortest half-life?

<p>IgG3 (C)</p> Signup and view all the answers

Which immunoglobulin types can cross the placenta?

<p>IgG1, IgG3, IgG4 (D)</p> Signup and view all the answers

Which immunoglobulin type has the lowest affinity for opsonization?

<p>IgG2 (C)</p> Signup and view all the answers

Which immunoglobulin types activate complement?

<p>IgG1, IgG2, IgG3 (A)</p> Signup and view all the answers

Which immunoglobulin type has the highest serum concentration?

<p>IgG1 (D)</p> Signup and view all the answers

What is the primary purpose of proficiency testing?

<p>To evaluate laboratory performance against established standards (D)</p> Signup and view all the answers

Who performs external quality control testing?

<p>Laboratory personnel using external specimens (B)</p> Signup and view all the answers

Which chart is commonly used for monitoring internal quality control data?

<p>Levey-Jennings Chart (B)</p> Signup and view all the answers

What distinguishes external quality control from internal quality control?

<p>External QC involves external specimens sent for testing (B)</p> Signup and view all the answers

What federal regulation pertains to proficiency testing?

<p>All laboratories providing results for human diagnosis must conduct proficiency testing (D)</p> Signup and view all the answers

Which of the following elements is NOT significantly affected by fist pumping during venipuncture?

<p>Calcium (D)</p> Signup and view all the answers

What is a consequence of using a tourniquet for more than one minute during venipuncture?

<p>Increased total protein levels (C)</p> Signup and view all the answers

Which process is likely to cause an increase in lactic acid levels in a blood sample?

<p>Fist pumping (C)</p> Signup and view all the answers

What is the expected effect of prolonged tourniquet application on calcium levels?

<p>Calcium levels may increase (C)</p> Signup and view all the answers

How does fist pumping affect blood pH during venipuncture?

<p>Decreases blood pH (C)</p> Signup and view all the answers

Flashcards

Proficiency Testing

A process where lab personnel analyze samples from an external agency to check accuracy and reliability.

Internal Quality Control

When a lab checks its own performance using control materials with known values.

Levey-Jennings Control Charts

A system of charts used to track and analyze the performance of lab controls over time.

Westgard Multirules

A set of rules used to determine if lab results are acceptable or indicate an issue.

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PDCA Cycle

A quality management framework that includes plan, do, check, and act steps to identify and address problems.

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E. histolytica

A single-celled parasite that can cause amoebiasis. Its infective stage is a quadrinucleated cyst.

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Giardia lamblia

A flagellated parasite that causes giardiasis. Its infective stage is a cyst, which is only found in humans.

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Toxoplasma

A protozoan parasite that causes toxoplasmosis. It can be found in soil as a sporulated oocyst, in cats as tachyzoites, and in undercooked meat as bradyzoites.

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Echinococcus granulosus

A tapeworm that can cause hydatid disease. Its infective stage is an embryonated egg.

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Schistosoma hematobium

A fluke that causes schistosomiasis. Its infective stage is a cercaria larva.

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IgG1 - Most Abundant

IgG1 is the most abundant immunoglobulin in the blood. It is able to cross the placenta and activate the complement system.

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IgG2 - Placenta Pass?

IgG2 is present in lower levels than IgG1 and doesn't cross the placenta. It activates complement but has lower affinity for phagocytes.

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IgG3 - Short Half-Life

IgG3 has a shorter half-life than IgG1 and IgG2, but it is an effective activator of complement.

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IgG4 - No Complement Activator

IgG4 does not activate complement and has a moderate affinity for phagocytes.

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IgG Serum Concentration Order

These serum concentrations indicate the relative abundance of each IgG type in the blood.

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Fist Pumping

The process of repeatedly clenching and unclenching the fist during venipuncture.

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Effects of Fist Pumping

Increased levels of potassium, lactic acid, calcium, and phosphorus in the blood, and a lower pH.

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Serological tests

A type of blood test that measures the presence of antibodies to specific diseases in a blood sample.

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Parasitological Test

A common test used to diagnose a parasitic infection in a patient.

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Blood tests

A test that analyzes a sample of blood to figure out if the patient has a specific disease or condition.

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Transmitted Light Microscope

A microscope that uses light that passes through the specimen, illuminating it from below.

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Darkfield Microscope

A type of microscope that uses light directed at the specimen from the side, creating a bright image against a dark background.

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Phase Contrast Microscope

A type of microscope that uses specialized optics to enhance the contrast of transparent specimens, allowing for clearer visualization of details.

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Electron Microscope

A powerful microscope that uses a beam of electrons instead of light to create high-resolution images of small objects.

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Light Microscope

A standard microscope that illuminates the specimen from below using a light source and lenses to magnify the image.

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Study Notes

Proficiency Testing

  • Proficiency testing is involved in external quality control
  • Internal QC is performed by lab personnel using control materials of known values
  • Control material values are assessed using Levy-Jennings control charts and Westgard multirules
  • External QC is performed when samples are submitted to an external agency
  • Results are submitted to the agency for assessment
  • This type of assessment is known as proficiency testing
  • It is required by federal regulations for all laboratories providing results for human diagnosis and/or treatment

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