General Microbiology & Immunology Notes (Part I)
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Questions and Answers

What is the main origin of tertiary cell cultures?

  • Animal tissue
  • Cancer tissue (correct)
  • Plant tissue
  • Bacterial culture
  • What cellular process is involved in the penetration of a virus into a host cell?

  • Osmosis
  • Active transport
  • Exocytosis
  • Receptor-mediated endocytosis (correct)
  • Which step of viral replication involves the removal of the viral capsid?

  • Replication
  • Uncoating (correct)
  • Attachment
  • Assembly
  • What type of organisms do fungi belong to?

    <p>Non-photosynthetic eukaryotes (D)</p> Signup and view all the answers

    How are naked viruses typically released from the host cell?

    <p>Lysis of the infected host cell (C)</p> Signup and view all the answers

    Which type of fungi primarily decompose plant material in soil and water?

    <p>Moulds (A)</p> Signup and view all the answers

    Where do most DNA viruses assemble within the host cell?

    <p>Nucleus (B)</p> Signup and view all the answers

    What characteristic do all fungi share regarding their nutritional needs?

    <p>They require organic compounds. (C)</p> Signup and view all the answers

    What was the main criterion used to distinguish the plant kingdom from the animal kingdom?

    <p>Presence of chlorophyll (C)</p> Signup and view all the answers

    Which type of organism is NOT included in the kingdom Protista?

    <p>Bacteria (D)</p> Signup and view all the answers

    Which of the following is a characteristic unique to eukaryotic cells?

    <p>Complex cellular organization (B)</p> Signup and view all the answers

    What energy source do members of the plant kingdom primarily utilize?

    <p>Light through photosynthesis (C)</p> Signup and view all the answers

    Which of these statements about microorganisms is NOT true?

    <p>They are included only in the kingdom Protista. (B)</p> Signup and view all the answers

    Which protist is characterized by containing chlorophyll?

    <p>Algae (C)</p> Signup and view all the answers

    Which of these organisms share features with both fungi and protozoa?

    <p>Slime moulds (D)</p> Signup and view all the answers

    What was a significant result of the introduction of the electron microscope?

    <p>Ability to classify prokaryotes and eukaryotes (C)</p> Signup and view all the answers

    What does the tRNA molecule specifically bind to during protein synthesis?

    <p>Codons on mRNA (C)</p> Signup and view all the answers

    Which of the following statements is true regarding ribosomal RNA (rRNA)?

    <p>rRNA is formed from a large precursor molecule that is enzymatically cleaved (C)</p> Signup and view all the answers

    What is the primary role of the promoter in a gene?

    <p>To initiate the transcription process (B)</p> Signup and view all the answers

    In eukaryotes, what distinguishes exons from introns?

    <p>Exons contain coding information while introns are non-coding sequences. (D)</p> Signup and view all the answers

    What defines the template strand of DNA?

    <p>It contains the controlling elements for gene regulation. (B)</p> Signup and view all the answers

    What characterizes obligate aerobes in terms of oxygen requirement?

    <p>They require atmospheric oxygen for growth. (D)</p> Signup and view all the answers

    Which enzyme is absent in obligate anaerobes that contributes to the accumulation of hydrogen peroxide in the presence of oxygen?

    <p>Superoxide dismutase (A), Catalase (B)</p> Signup and view all the answers

    How do facultative anaerobes adapt their metabolism?

    <p>They switch between fermentation and respiration as needed. (A)</p> Signup and view all the answers

    What is the primary condition that microaerophiles require for growth?

    <p>Low tension of oxygen (C)</p> Signup and view all the answers

    Which of these minerals is crucial for the synthesis of cytochromes?

    <p>Iron (D)</p> Signup and view all the answers

    Which heavy metal ions are specifically required for various enzymatic reactions?

    <p>Copper, zinc, and magnesium (B)</p> Signup and view all the answers

    What happens to microaerophilic organisms in high oxygen tension conditions?

    <p>They accumulate hydrogen peroxide and can die. (B)</p> Signup and view all the answers

    What role does sulfur play in cellular processes?

    <p>It is a component of certain amino acids. (C)</p> Signup and view all the answers

    What is the function of conidiophores in fungi?

    <p>They are specialized for the formation of asexual spores. (C)</p> Signup and view all the answers

    Which class of fungi is characterized by having no known sexual process?

    <p>Deuteromycotina (A)</p> Signup and view all the answers

    What structure is responsible for the formation of basidiospores?

    <p>Basidium (C)</p> Signup and view all the answers

    Which of the following is an example of a dimorphic fungus?

    <p>Histoplasma capsulatum (C)</p> Signup and view all the answers

    What characteristic defines slime moulds during their life cycle?

    <p>Presence of a plasmodium stage. (A)</p> Signup and view all the answers

    Which type of asexual spores are characterized by being formed from segments of a septated mycelium?

    <p>Arthrospores (D)</p> Signup and view all the answers

    What is the primary advantage of dimorphism in fungi?

    <p>Enables rapid reproduction in adverse conditions. (D)</p> Signup and view all the answers

    Which type of fungal spores are borne inside a sac and are formed on aerial hyphae?

    <p>Sporangiospores (A)</p> Signup and view all the answers

    What is the primary function of oxidative phosphorylation?

    <p>Synthesis of ATP through electron transport (D)</p> Signup and view all the answers

    Which statements accurately describe photophosphorylation?

    <p>It requires chlorophyll to oxidize light-sensitive pigments. (C)</p> Signup and view all the answers

    What role do coenzymes such as NAD and FAD play in metabolic reactions?

    <p>They act as electron carriers during oxidation and reduction. (C)</p> Signup and view all the answers

    Which of the following processes conserves free energy from the oxidation of organic molecules?

    <p>Both substrate level phosphorylation and oxidative phosphorylation (B)</p> Signup and view all the answers

    What is the estimated amount of ATP synthesized from the oxidation of 1 mole of glucose to CO2 and water?

    <p>38 moles of ATP (A)</p> Signup and view all the answers

    Which of the following best describes chemoorganotrophic metabolism?

    <p>It entails the oxidation of organic molecules to generate energy. (D)</p> Signup and view all the answers

    During ATP synthesis, which of the following statements is true?

    <p>Some substances are oxidized while others are reduced. (B)</p> Signup and view all the answers

    What is the significance of the free energy released during electron transport?

    <p>It powers the synthesis of ATP molecules. (C)</p> Signup and view all the answers

    Flashcards

    Two-Kingdom Classification

    The initial classification of living organisms, before the discovery of microorganisms, categorized organisms into two kingdoms: Animal and Plant. This classification relied on structural and physiological characteristics such as energy source, presence of chlorophyll, reserve material, movement, cell wall, and growth mode.

    Kingdom Protista

    A third kingdom, Protista, was proposed by Haeckel in 1866 to accommodate microorganisms. It included unicellular or coencytic, and multicellular organisms that lacked specialized cell types and tissues.

    Impact of Microorganisms on Classification

    The discovery of microorganisms led to the need for a new kingdom to classify them, as they didn't fit neatly into the existing animal and plant kingdoms.

    Electron Microscope and Cell Differentiation

    The introduction of the electron microscope allowed scientists to differentiate between prokaryotic and eukaryotic cells based on their internal structure.

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    Prokaryotes vs. Eukaryotes

    Eukaryotic cells have a nucleus and other membrane-bound organelles, while prokaryotic cells lack a nucleus and have simpler internal structures.

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    Revised Kingdom Protista

    The classification of Kingdom Protista was revised after the discovery of prokaryotes. Protista now only encompasses eukaryotic organisms, while prokaryotes were placed in their own distinct groups.

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    Algae

    Algae are chlorophyll-containing protists that can produce their own food through photosynthesis.

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    Protozoa

    Protozoa are nonphotosynthetic, heterotrophic eukaryotic organisms that lack a cell wall. They are typically unicellular and obtain nutrition by consuming other organisms.

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    Continuous Cell Lines

    Cells derived from cancer tissue, capable of continuous division in culture.

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    Chick Embryo Culture

    A method of cultivating viruses in fertilized chicken eggs, often used for producing vaccines.

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    Viral Attachment

    The process of a virus attaching to a host cell's surface.

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    Receptor-mediated Endocytosis

    A method of viral entry into a host cell where the cell's membrane engulfs the virus.

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    Membrane Fusion (Viral Entry)

    The process of a virus fusing its envelope with the host cell's membrane to enter.

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    Viral Uncoating

    The process of a virus removing its outer coat to release genetic material.

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    Viral Replication

    The replication process where viral genetic material is used to produce viral proteins and new viruses.

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    Viral Release

    The final stage of viral replication where new viruses are assembled and released from the host cell.

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    What are Ascomycetes?

    Fungi that reproduce sexually by forming spores inside a sac-like structure called an ascus.

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    What is a basidium?

    A club-shaped structure in Basidiomycetes where sexual spores (basidiospores) are formed.

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    What are Deuteromycetes?

    Fungi that lack a known sexual reproduction process.

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    What are sporangiospores?

    Asexual spores produced within a sac called a sporangium, formed at the tip of a specialized hypha called a sporangiophore.

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    What are conidia?

    Asexual spores formed on the ends or sides of hyphae or special structures called conidiophores.

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    What are arthrospores?

    Conidia that are formed by splitting a filament of a septated mycelium into individual cells.

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    What are chlamydospores?

    Thick-walled, enlarged conidia formed inside hyphae.

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    What are blastospores?

    Blastic spores that bud off from a parent cell and then separate.

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    Strict Aerobes

    These organisms require oxygen to grow and cannot survive without it.

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    Strict Anaerobes

    These organisms cannot grow in the presence of oxygen and are harmed by it.

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    Facultative Anaerobes

    These organisms can grow with or without oxygen.

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    Microaerophiles

    These organisms need a small amount of oxygen to grow, but too much is harmful.

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    Sulfur

    An essential element for the synthesis of certain amino acids, like cysteine, and coenzymes.

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    Iron

    Needed for the production of cytochromes, critical for energy production.

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    Heavy Metal Ions (Mg, Zn, Co, Cu, Mn)

    These minerals play vital roles in various enzymatic reactions within cells.

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    Phosphorus

    Crucial for the synthesis of nucleotides, nucleic acids, and lipids, essential for cell structure and function.

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    Oxidative Phosphorylation

    The process of ATP synthesis driven by the movement of electrons through a membrane-bound electron transport system (ETS). Energy released from electron transport is used to power ATP production.

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    Photophosphorylation

    ATP synthesis using electrons from chlorophyll, powered by solar energy. Similar to oxidative phosphorylation, but driven by light.

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    Chemoorganotrophic Metabolism

    The oxidation of organic molecules (like carbohydrates) to release energy and generate ATP. This is a common metabolic pathway in many organisms.

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    Substrate-Level Phosphorylation

    A process where ATP is directly generated during the breakdown of organic molecules, without involving electron transport systems.

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    Reduced

    Molecules that gain electrons in a chemical reaction.

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    Oxidized

    Molecules that lose electrons in a chemical reaction.

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    Electron Carriers

    Electron carriers, like NAD and FAD, play a crucial role in transporting electrons during metabolic reactions. This is essential for generating ATP.

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    Glucose Oxidation to ATP

    The energy released from the oxidation of glucose is stored in ATP, with approximately 38 ATP molecules generated per mole of glucose.

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    Transcription

    The process by which RNA polymerase reads DNA and creates a complementary RNA strand. It starts at a promoter site on the DNA and continues until it reaches a termination codon.

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    Ribosomal RNA (rRNA)

    A type of RNA that forms part of the ribosome, the cellular machinery responsible for protein synthesis.

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    Transfer RNA (tRNA)

    A type of RNA that carries specific amino acids to the ribosome during protein synthesis. Each tRNA has an anticodon that matches a specific codon on mRNA, ensuring the correct amino acid is incorporated into the growing protein chain.

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    Gene

    The basic unit of inheritance in a living organism. It is a sequence of DNA that encodes a specific protein, tRNA, or rRNA. Genes have a defined start and end point and contain control elements that regulate their expression.

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    Study Notes

    General Microbiology & Immunology Notes (Part I)

    • Microbiology is the study of minute or small organisms not visible to the naked eye.
    • Antonie van Leeuwenhoek, known as the "Father of Microbiology", was the first person to observe microorganisms using a simple microscope.
    • He observed small swimming organisms in pond water, calling them "animalcules."
    • The theory of spontaneous generation (abiogenesis) proposed that life arose from non-living matter.
    • Francesco Redi challenged this theory by demonstrating that maggots on decaying meat came from fly eggs, not the meat itself.
    • Lazzaro Spallanzani further challenged spontaneous generation by showing that sealed and boiled flasks did not develop microorganisms, suggesting that air carried germs.
    • John Needham, however, countered Spallanzani's experiment by stating that vital force was required.
    • Louis Pasteur definitively disproved spontaneous generation by demonstrating that broth in swan-necked flasks remained sterile, even though exposed to air.
    • He developed the process of pasteurization to prevent spoilage in liquids.
    • Edward Jenner pioneered the concept of vaccination by demonstrating that cowpox infection provides immunity to smallpox.
    • Robert Koch established postulates for establishing a link between a microorganism and a specific disease. These include proving the microorganism is present in every case of the disease; isolating and culturing the microbe; re-introducing the isolated microorganism into a healthy animal to cause the disease; and isolating the microorganism again from this diseased animal to show its identity.
    • Koch also developed culture media and techniques for isolating and cultivating microbes, and identified many pathogens.
    • Koch's laboratory helped make significant advancements in microbiology with discoveries like the petri dish, the use of agar, and the gram stain.
    • Julius Petri developed the Petri dish.
    • Frau Hesse introduced agar.
    • Christian Gram developed the Gram stain.
    • Paul Ehrlich pioneered chemotherapy using arsphenamine, a 'magic bullet' for treating certain infections.
    • Joseph Lister introduced antiseptic surgery by using phenol.

    Development of Microbiology

    • Before the discovery of microorganisms, all living organisms were classified into two kingdoms: Animalia and Plantae.
    • Criteria differentiating plant and animal kingdoms included energy source (light vs. oxidation), presence of chlorophyll, principal reserve materials (starch vs. glycogen), active movement, and cell wall.
    • The kingdom Protista was proposed by Haeckel in 1866
    • This kingdom comprised unicellular and multicellular organisms that lacked specialization/differentiation of cell types and tissues.
    • The kingdom Protista was later modified to include only eukaryotic organisms like algae/chlorophyll containing protists.
    • Protozoa were grouped as non-photosynthetic heterotrophic, unicellular eukaryotes.
    • Fungi are non-photosynthetic eukaryotes with cell walls.
    • Slime molds share features with fungi and protozoa.
    • A major taxonomy differentiation occurred with three kingdoms. Eubacteria, Archaea and Eucarya.

    Classification of Living Organisms

    • Currently, a system of classification using three domains has become prevalent
    • Domain Protista includes unicellular eukaryotes like algae. Protozoa, fungi, algae and bacteria were all categorized under this kingdom for a time

    Viruses

    • Viruses are submicroscopic infectious agents, made of genetic material (DNA or RNA) surrounded by a protein coat (capsid).
    • They are obligate intracellular parasites, replicating only inside living cells.
    • Viruses can have icosahedral, helical, or complex symmetry.
    • Viral cultivation methods include tissue cultures (primary and secondary) and animal inoculation. Steps in viral replication include; attachment, penetration, uncoating, replication, assembly, and release.

    Fungi

    • Fungi are non-photosynthetic eukaryotic organisms that are heterotrophic, requiring organic compounds for energy and carbon.
    • They are commonly aerobic or facultatively anaerobic saprophytes, and frequently decompose plant material.
    • Fungi form a mass of branching filaments called hyphae, which together form a mycelium in some fungi.
    • Yeasts are unicellular fungi that reproduce by budding.

    Protozoa

    • Protozoa are unicellular, non-photosynthetic, chemoheterotrophic eukaryotes lacking cell walls.
    • They move by cilia or pseudopods and reproduce asexually or sexually.
    • Different groups of protozoa including the sarcodina, ciliophora, mastigophora and sporozoa

    Bacteria

    • Bacteria are a diverse group of prokaryotic microscopic organisms.
    • They inhabit various environments and play essential ecological roles (e.g., nutrient recycling).
    • Bacteria have a cell wall containing peptidoglycan (except for mycoplasmas).
    • Bacteria reproduce by binary fission, except for actinomycetes, which form conidiospores.
    • They have three fundamental forms: spherical (coccus), rod-shaped (bacillus), and curved/spiral (vibrio, spirillum, spirochaete)

    Bacterial Cell Structure

    • A typical bacterium has a cell wall, cell membrane and cytoplasm containing ribosomes, genetic material and other cytoplasmic components like slime layer or capsule.
    • Some types of bacteria have dormant spores under adverse environmental conditions.
    • The nucleoid carries the cell’s genetic information as a single circular DNA molecule.
    • The cytoplasm is an aqueous mixture containing nutrients, metabolites, waste products, enzymes, and reserve food.
    • The cell membrane functions in selective permeability, transport, excretion, and biosynthesis. Note: Additional information in the text, like sections on the cell wall, bacterial morphology, special types of bacteria and/or other structural components, are not included in this summary.

    Bacterial Metabolism

    • Metabolism involves anabolism (building complex molecules) and catabolism (breaking down complex molecules).
    • Organisms may obtain their energy via phototrophy (light), or chemotrophy (chemical reactions using organic or inorganic molecules). Different types of chemotrophs exist.
    • Most eukaryotic cells and bacteria carry out chemoorganotrophic metabolism by the oxidation of organic molecules.
    • The oxidation of organic molecules produces ATP via various pathways such as substrate-level phosphorylation and oxidative phosphorylation.
    • Oxygen, nitrate, or other inorganic molecules may serve as the final electron acceptors during respiration.
    • When oxygen is not available or when the organism cannot use respiration (e.g., in some bacteria), fermentation occurs.

    Nucleic Acids/DNA

    • Nucleic acids (DNA and RNA) carry genetic material.
    • DNA has a double helix structure with four bases (adenine, guanine, cytosine, and thymine).
    • RNA has a single-stranded structure with four bases (adenine, guanine, cytosine, and uracil).

    DNA Replication

    • DNA replication is semi-conservative; each new DNA molecule contains one original strand and one new strand
    • It typically occurs from a specific origin of replication and proceeds bidirectionally; Replication is typically bi-directional.
    • DNA polymerases are involved in copying the DNA
    • Two growing strands are involved, one leading and the other lagging; DNA nucleotides are added to the 3' end of a growing DNA chain.

    Transcription and Prokaryotic Gene Expression

    • Transcription is the synthesis of RNA using DNA as a template. This process involves three stages - initiation, elongation, and termination.
    • Prokaryotic organisms have only one RNA polymerase consisting of multiple subunits.
    • Gene expression is regulated with constitutive enzymes present at relatively constant levels and induced enzymes produced only when substrates are present.

    Translation

    • Translation is the synthesis of proteins using mRNA as a template. It follows three steps - Initiation, Elongation, and Termination
    • Ribosomes that are composed of RNA and protein are essential for this process

    Regulation of Gene Expression

    • Regulation of gene expression controls the levels of proteins being produced.
    • Mechanisms include; induction (an increase in enzyme level) with an inducer; end product repression (decrease in enzyme level) by the effect of an end product) as well as feedback inhibition (the end product of the pathway inhibits the first enzyme)
    • Genes and operons are controlled with regulatory proteins.

    Bacterial Growth and Growth Curve

    • Some prokaryotes grow using a closed system, where the medium and growth is contained in a batch culture.
    • In a batch culture, bacterial growth is best characterized by four phases: the lag phase, log phase, stationary phase, and death phase.
    • An open system, like a continuous culture system, is a more controllable method for measuring cell growth.

    Microbial Variation

    • Variation in microorganisms is an important aspect of adaptation and evolution
    • Genotype or phenotype changes can be reversible or irreversible.
    • Mutation is a permanent change in the genetic material (DNA)
    • Environmental factors, like radiation or chemicals, can induce mutations.
    • Organisms have repair mechanisms to fix DNA damage

    Microbial Control,

    • A variety of methods are used to control microorganisms in various environments.

    Genetic Transfer

    • Genes can be transferred vertically (from parent to offspring) or horizontally (among different organisms).
    • Horizontal gene transfer (HGT) in microorganisms occurs through transformation, conjugation, and transduction.

    Microbial Growth and Measurement

    • Microbial growth is measured using various methods, including; observing cell mass (dry weight or optical density); and viable cell counts (based on the number of live cells).

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    Description

    Explore the foundational concepts of microbiology and immunology in this quiz. Learn about key figures such as Antonie van Leeuwenhoek and Louis Pasteur, as well as important theories like spontaneous generation. This quiz will test your understanding of the early discoveries that shaped the field of microbiology.

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