Microbiology and DNA Repair
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Questions and Answers

Which of the following is used as a counterstain in the acid fast stain technique?

  • Alcohol
  • Crystal violet
  • Methylene blue
  • Safranin (correct)
  • Which stain is primarily used to identify Mycobacterium tuberculosis?

  • Ziehl neelsen stain (correct)
  • Eosin stain
  • Giemsa stain
  • Gram stain
  • What is the role of methylene blue in the Ziehl neelsen stain method?

  • Primary stain
  • Decolorizer
  • Counterstain (correct)
  • Fixative
  • Which process involves post replication repair mechanisms?

    <p>All of these options</p> Signup and view all the answers

    Which substance is used as a primary stain in the Ziehl neelsen method?

    <p>Carbol fuchsin</p> Signup and view all the answers

    Study Notes

    Mycobacterium tuberculosis

    • Mycobacterium tuberculosis is identified using acid-fast stain, which involves two dyes: crystal violet and safranin.
    • Another method used to identify Mycobacterium tuberculosis is the Ziehl-Neelsen stain, which involves the following steps: decolorization with alcohol, and then counterstaining with methylene blue, followed by staining with carbol fuchsin.

    DNA Repair

    • Post-replication repair is a mechanism of DNA repair that occurs after DNA replication.
    • DNA repair involves the removal of lesions, which are damaged areas of DNA.
    • Excision repair is a type of DNA repair that involves the removal of damaged DNA nucleotides.
    • Recombination repair is a type of DNA repair that involves the exchange of genetic material between two DNA molecules.

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    Description

    This quiz covers the detection of Mycobacterium tuberculosis using acid-fast stains and Ziehl-Neelsen stain, as well as various mechanisms of DNA repair including post-replication repair and excision repair.

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