5 Questions
Which of the following is used as a counterstain in the acid fast stain technique?
Safranin
Which stain is primarily used to identify Mycobacterium tuberculosis?
Ziehl neelsen stain
What is the role of methylene blue in the Ziehl neelsen stain method?
Counterstain
Which process involves post replication repair mechanisms?
All of these options
Which substance is used as a primary stain in the Ziehl neelsen method?
Carbol fuchsin
Study Notes
Mycobacterium tuberculosis
- Mycobacterium tuberculosis is identified using acid-fast stain, which involves two dyes: crystal violet and safranin.
- Another method used to identify Mycobacterium tuberculosis is the Ziehl-Neelsen stain, which involves the following steps: decolorization with alcohol, and then counterstaining with methylene blue, followed by staining with carbol fuchsin.
DNA Repair
- Post-replication repair is a mechanism of DNA repair that occurs after DNA replication.
- DNA repair involves the removal of lesions, which are damaged areas of DNA.
- Excision repair is a type of DNA repair that involves the removal of damaged DNA nucleotides.
- Recombination repair is a type of DNA repair that involves the exchange of genetic material between two DNA molecules.
This quiz covers the detection of Mycobacterium tuberculosis using acid-fast stains and Ziehl-Neelsen stain, as well as various mechanisms of DNA repair including post-replication repair and excision repair.
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