Microbiology: Anaerobes Quiz

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Questions and Answers

What characterizes obligate anaerobes?

  • They require a minimum of 5% oxygen concentration for growth.
  • They can survive in aerobic conditions.
  • Oxygen is toxic to them. (correct)
  • They use oxygen as a terminal electron acceptor.

Which type of anaerobes can survive in the presence of oxygen but do not use it for energy production?

  • Aerotolerant anaerobes (correct)
  • Exogenous anaerobes
  • Obligate anaerobes
  • Facultative anaerobes

What is a common characteristic of anaerobes regarding oxygen levels?

  • Most can tolerate 3%-5% oxygen concentration. (correct)
  • They all require at least 10% oxygen for growth.
  • They thrive in high oxygen environments.
  • They can reproduce effectively in pure oxygen.

Where are anaerobes typically found in the human body?

<p>In the intestinal tract. (B)</p> Signup and view all the answers

Which of the following is an example of an endogenous anaerobe?

<p>Bacteroides fragilis (D)</p> Signup and view all the answers

Which anaerobic bacterium is known for causing tetanus?

<p>Clostridium tetani (B)</p> Signup and view all the answers

What is the primary method through which anaerobes generate energy?

<p>Fermentation (C)</p> Signup and view all the answers

Which of the following bacterial genera is NOT typically classified as an anaerobe?

<p>Escherichia (C)</p> Signup and view all the answers

Where do the spores of Clostridium tetani commonly enter the body?

<p>Through accidental puncture wounds (C)</p> Signup and view all the answers

Which toxin produced by Clostridium tetani is highly lethal and stable?

<p>Tetanospasmin (C)</p> Signup and view all the answers

What is the earliest symptom of tetanus?

<p>Trismus (lock jaw) (D)</p> Signup and view all the answers

What does the term 'opisthotonos' refer to in relation to tetanus symptoms?

<p>A form of rigidity in the back (C)</p> Signup and view all the answers

What treatment options are available for a patient with tetanus?

<p>Cleansing of wounds and antibiotic therapy (A)</p> Signup and view all the answers

What is the fatality rate associated with severe cases of tetanus?

<p>10-70% (D)</p> Signup and view all the answers

What type of organism is Clostridium tetani classified as?

<p>Obligate anaerobe (B)</p> Signup and view all the answers

Which of the following is a form of tetanus characterized by spasms that spread upward along the spinal cord?

<p>Ascending tetanus (B)</p> Signup and view all the answers

What condition is primarily associated with Clostridium perfringens?

<p>Gas gangrene (A)</p> Signup and view all the answers

Which factor is NOT a predisposing factor for anaerobic cellulitis or myonecrosis?

<p>High blood pressure (B)</p> Signup and view all the answers

What is a notable virulence factor of Clostridium difficile?

<p>Cytotoxin (Toxin B) (B)</p> Signup and view all the answers

Which of the following clinical signs is associated with gas gangrene?

<p>Blackened necrotic tissue filled with bubbles of gas (B)</p> Signup and view all the answers

What is a key management step for antibiotic-associated colitis caused by C. difficile?

<p>Discontinue antibiotics (C)</p> Signup and view all the answers

Which toxin is associated with pseudomembranous colitis?

<p>Toxin A (B)</p> Signup and view all the answers

What is the primary method for diagnosing Clostridium difficile infection?

<p>Stool sample toxin detection (A)</p> Signup and view all the answers

Which group of individuals is at a higher risk for developing tetanus?

<p>Agricultural workers and gardeners (A)</p> Signup and view all the answers

What is the recommended vaccination schedule for babies aged 1 to 3 months?

<p>Three injections given 2 months apart (A)</p> Signup and view all the answers

Which type of food poisoning is caused by C.perfringens type A?

<p>Watery diarrhea and acute abdominal pain (B)</p> Signup and view all the answers

What is a key characteristic of the botulism toxin produced by C.botulinum?

<p>It is a neurotoxic protein that causes flaccid paralysis (D)</p> Signup and view all the answers

What is a common source of infant botulism?

<p>Raw honey (B)</p> Signup and view all the answers

Which statement about the heat resistance of botulinum spores is true?

<p>They can survive 100C for 3-5 hours (B)</p> Signup and view all the answers

What is the incubation period for foodborne botulism?

<p>1-2 days (D)</p> Signup and view all the answers

What are common symptoms of botulism?

<p>Diplopia and dysphagia (D)</p> Signup and view all the answers

What factor can reduce the heat resistance of botulinum spores?

<p>Acid pH or high salt concentrations (B)</p> Signup and view all the answers

What is the most common clinical disease caused by Lactobacillus acidophilus?

<p>Endocarditis (B)</p> Signup and view all the answers

Which of the following is NOT appropriate for anaerobic culture specimen collection?

<p>Blood (A)</p> Signup and view all the answers

What is a key characteristic of Lactobacillus acidophilus colonies when grown on SBA?

<p>Pin-point alpha hemolytic colonies (A)</p> Signup and view all the answers

What type of antibiotic resistance is commonly observed in Lactobacillus acidophilus?

<p>Cephalosporins (B)</p> Signup and view all the answers

Which organism is considered the most pathogenic anaerobic cocci?

<p>Finegoldia magna (C)</p> Signup and view all the answers

What should be done immediately upon arrival of a specimen for anaerobic culture in the lab?

<p>Place it in an anaerobic chamber (C)</p> Signup and view all the answers

What is essential for transporting anaerobic specimens?

<p>Avoid exposure to oxygen (D)</p> Signup and view all the answers

What method is used for Gram staining in anaerobic cultures?

<p>Standard Gram stain method (B)</p> Signup and view all the answers

What immediate effect does the germination of spores in the intestine have?

<p>Releases a neurotoxin (D)</p> Signup and view all the answers

Which symptom is NOT associated with infant botulism?

<p>Severe headache (C)</p> Signup and view all the answers

What is a primary treatment method for botulism?

<p>Type A, B, and E trivalent antitoxins (B)</p> Signup and view all the answers

What chronic condition is characterized by the formation of sinus tracts and fistulae?

<p>Actinomycosis (D)</p> Signup and view all the answers

What type of discharge is typically associated with bacterial vaginosis?

<p>Gray-white and malodorous (B)</p> Signup and view all the answers

Which of the following functions does Lactobacillus perform in the female genital tract?

<p>Lowers vaginal pH (C)</p> Signup and view all the answers

Which bacterial genus is associated with an overgrowth in bacterial vaginosis?

<p>Gardnerella (B)</p> Signup and view all the answers

What is a common risk factor for wound botulism?

<p>Drug abuse (C)</p> Signup and view all the answers

Flashcards

Anaerobes

Bacteria that can replicate without oxygen.

Obligate Anaerobe

Anaerobe that cannot survive in the presence of oxygen.

Aerotolerant Anaerobe

Anaerobe that can tolerate oxygen but doesn't use it for energy production.

Facultative Anaerobe

Anaerobe that can grow with or without oxygen, using oxygen if available.

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Exogenous Anaerobic Infection

Infection caused by anaerobes already present outside the body.

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Endogenous Anaerobic Infection

Infection caused by anaerobes already present inside the body.

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Oxygen Toxicity

Harmful effects of oxygen on anaerobes due to the formation of reactive oxygen species (ROS).

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Anaerobic Environment

An environment with little to no oxygen.

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Gas gangrene

A serious infection caused by anaerobic bacteria, leading to tissue damage, gas production, and potential death.

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Clostridia in infections

Bacteria that cause various diseases, including wound infections, food poisoning, and myonecrosis.

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Septicum

A bacterial marker potentially associated with gastrointestinal malignancy.

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Antibiotic-associated colitis

Inflammation of the colon caused by an antibiotic, often by C. difficile.

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Virulence factors (C. difficile)

Microbes like C. difficile possess toxins (A & B) causing disease.

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Tetanus

A serious nervous system infection, often caused by spores.

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Predisposing factors (wound infections)

Conditions making one more vulnerable to infections in injuries, e.g., surgical sites, or diabetic wounds.

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Treatment for Antibiotic-Associated Colitis

Stopping the antibiotic, administering metronidazole or vancomycin, often orally, is critical for managing the disease.

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Tetanus

A serious neuromuscular disease caused by Clostridium tetani bacterium.

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Clostridium tetani

A bacterium that produces toxins causing tetanus.

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Tetanospasmin

The highly lethal toxin produced by Clostridium tetani, causing muscle spasms.

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Trismus

A symptom of tetanus; difficulty opening the jaw.

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Opisthotonos

Severe back arching caused by tetanus.

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Treatment for Tetanus

Includes supportive care, antimicrobial drugs, muscle relaxants and antitoxin.

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Incubation period of Tetanus

Time between infection and symptom appearance. Typically 4-10 days.

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Prevention of Tetanus

Immunization with tetanus toxoid (vaccine) and the use of immunoglobulin.

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Neonatal tetanus prevention

Vaccination of pregnant women protects newborns from tetanus.

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Clostridial food poisoning

Food poisoning caused by C. perfringens bacteria, leading to diarrhea and abdominal pain.

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Botulism toxins

Neurotoxic proteins produced by C. botulinum bacteria preventing acetylcholine release.

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Infant botulism

Botulism caused by C. botulinum spores germinating and producing toxin in the body.

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Botulism diagnosis

Diagnose by isolating the organism in food/faeces or detecting toxin in faeces/serum.

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Botulism characteristics

Foodborne botulism (1-2 day incubation) vs. Wound botulism (>4 days) and symptoms include diplopia, dysphagia etc.

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Heat resistance of C.botulinum spores

Spores of this bacteria withstand high temperatures (100C for 3-5 hrs), but are less resistant to acid and high salt.

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Botulism incubation period

Symptoms typically appear 12–36 hours after toxin ingestion for food borne botulism.

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Infant Botulism

A form of botulism caused by spores ingested by infants, leading to flaccid paralysis and weak sucking.

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Wound Botulism

Botulism resulting from spores entering a wound, leading to symptoms similar to foodborne botulism.

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Botulism Treatment

Involves antitoxins, respiratory support, and penicillin, with attention to preserved foods.

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Actinomycosis

A chronic infection with sinus tracts and fistulae, potentially with sulfur granules, often in the maxilla or female genital tract.

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Bacterial Vaginosis

Vaginal infection caused by overgrowth of certain anaerobic bacteria, with a characteristic gray-white discharge.

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Lactobacillus

Gram-positive bacteria vital for vaginal health, producing lactic acid to lower pH and prevent overgrowth of other bacteria.

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Spores in Botulism

Spores of the bacterium Clostridium botulinum present in common environments cause botulism.

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Symptoms of Botulism

Botulism causes flaccid paralysis, weakness, respiratory issues, and a decreased response in infants' sucking.

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Lactobacillus acidophilus

A type of bacteria commonly found in the mouth, gut, and female genital tract; often resistant to certain antibiotics.

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Clinical infections from anaerobic gram-negative bacilli

Infections (e.g., peritoneal infections, septicemia, abscesses) caused by anaerobic gram-negative bacteria.

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Finegoldia magna

A pathogenic anaerobic coccus, often found in pure culture, involved in various infections.

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Appropriate anaerobic specimen collection

Collecting samples for anaerobic bacteria with minimal exposure to oxygen, using a 2 tube system with O2-free environment and PRAS.

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Anaerobic transport method

Moving samples to the lab while maintaining an anaerobic environment to prevent oxygen contact and preserve bacteria types.

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Anaerobic laboratory processing

Immediately processing anaerobic specimens in an anaerobic chamber to maintain and allow for visualization and culturing of bacteria types.

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Anaerobic culture

Cultivation of bacteria in the absence of oxygen to encourage growth and identification, which is necessary for successful treatment.

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Specimen types for anaerobic cultures

Specific body fluids (e.g., pus, pleural fluid, sputum) suitable for identifying anaerobic bacteria.

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