Microbiology 101 Quiz
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Questions and Answers

During which phase of bacterial growth are the number of cells dividing and dying approximately equal?

  • Lag phase
  • Exponential phase
  • Stationary phase (correct)
  • Decline phase
  • Which bacterial structure is primarily associated with adherence to surfaces rather than motility?

  • Endospores
  • Pili (correct)
  • Flagella
  • Cocci
  • What is the primary characteristic of bacteria described as pleomorphic?

  • They are spherical
  • They are helical
  • Their shape varies (correct)
  • They are rod-shaped
  • Which of the following is a key trait of endospores that allows them to survive adverse conditions?

    <p>Resistance to drying and heat (B)</p> Signup and view all the answers

    Which bacterial morphology is characterized by a rod-like shape?

    <p>Bacilli (C)</p> Signup and view all the answers

    Which bacterial structure is known for its role in shielding the bacteria from immune responses?

    <p>Capsule (C)</p> Signup and view all the answers

    What is the primary function of lipopolysaccharide in Gram-negative bacteria?

    <p>To serve as an essential component of the outer membrane and act as a virulence factor (A)</p> Signup and view all the answers

    Which term describes the genetic material of all microbes living in and on the human body?

    <p>Microbiome (D)</p> Signup and view all the answers

    What characteristic of a biofilm is critical for its formation and function?

    <p>A complex of polysaccharide material is essential for its structure. (D)</p> Signup and view all the answers

    What is the most significant difference between microbiota and microbiome?

    <p>Microbiota refers to the community of microbes like bacteria, fungi, and viruses, and microbiome refers to their genetic material. (B)</p> Signup and view all the answers

    Which of the following best describes the cellular structure of prokaryotes?

    <p>Contain a nucleoid with a single circular chromosome and lack a nuclear membrane. (D)</p> Signup and view all the answers

    Which of these microorganisms is classified as a Gram-negative cocci?

    <p>Neisseria (B)</p> Signup and view all the answers

    In scientific nomenclature, which of the following is the correct way to write the species name?

    <p>Staphylococcus aureus (C)</p> Signup and view all the answers

    Which of the following is a defining characteristic of eukaryotic cells?

    <p>DNA organized into linear chromosomes within a nucleus. (C)</p> Signup and view all the answers

    Which of the following describes the cell wall of prokaryotic cells?

    <p>Rigid structures containing peptidoglycan and proteins. (C)</p> Signup and view all the answers

    Which of the following is a correct descending order of taxonomic classification?

    <p>Domain, Phylum, Class, Order (D)</p> Signup and view all the answers

    Which of the following is NOT a Gram-positive cocci?

    <p>Moraxella (C)</p> Signup and view all the answers

    What is the term for a genetic variant or subtype of a microorganism, such as E. coli O157:H7?

    <p>Strain (C)</p> Signup and view all the answers

    What is the primary purpose of the Gram stain in clinical microbiology?

    <p>To differentiate bacteria based on their cell wall structure. (C)</p> Signup and view all the answers

    Which component of Gram-positive bacteria contributes to their purple staining after the Gram stain procedure?

    <p>A thick peptidoglycan layer. (D)</p> Signup and view all the answers

    What is the role of iodine in the Gram staining process?

    <p>To act as a mordant, setting the crystal violet dye within the bacterial cell wall. (D)</p> Signup and view all the answers

    What is the function of the alcohol during the Gram stain procedure?

    <p>To remove the crystal violet from Gram-negative cells with thin peptidoglycan layers. (D)</p> Signup and view all the answers

    Which reagent is used as a counterstain in the Gram stain?

    <p>Safranin. (C)</p> Signup and view all the answers

    What is the expected color of Gram-negative bacteria after the complete Gram stain procedure?

    <p>Pink or Red (A)</p> Signup and view all the answers

    Why do acid-fast bacteria not stain well with the Gram stain method?

    <p>Their cell walls contain a waxy layer of glycolipids and fatty acids. (D)</p> Signup and view all the answers

    Which bacterial cell structure is directly responsible for the differential staining observed in Gram-positive and Gram-negative bacteria?

    <p>The thickness of the peptidoglycan layer. (C)</p> Signup and view all the answers

    Which characteristic is LEAST associated with the Bacillus genus?

    <p>Gram-variable staining (B)</p> Signup and view all the answers

    What is a primary concern with Clostridium perfringens infections?

    <p>Rapid tissue necrosis (B)</p> Signup and view all the answers

    Which Clostridium species is MOST likely to overgrow following prolonged antibiotic usage?

    <p>Clostridioides difficile (A)</p> Signup and view all the answers

    Which symptom is MOST specifically associated with Clostridium tetani?

    <p>Simultaneous muscle contraction and relaxation (A)</p> Signup and view all the answers

    Which of these options is a key factor that contributes to food botulism?

    <p>Ingestion of a neurotoxin (C)</p> Signup and view all the answers

    What is a defining Gram staining characteristic for both Bacillus and Clostridium species?

    <p>Gram-positive rods (D)</p> Signup and view all the answers

    What is a common method of transmission for Bacillus cereus food poisoning?

    <p>Unrefrigerated rice dishes (D)</p> Signup and view all the answers

    Which of these options is an element of treatment MOST specific to Clostridium tetani infection?

    <p>Neutralizing antitoxin (B)</p> Signup and view all the answers

    What is the primary function of soap in hand hygiene when disinfecting a surface or hands?

    <p>To remove organic material and loose skin cells. (B)</p> Signup and view all the answers

    Which of the following methods can achieve sterilization?

    <p>Moist heat, such as in an autoclave (D)</p> Signup and view all the answers

    When performing hand hygiene, what is the first step according to the instructions?

    <p>Turn on water and wet hands (B)</p> Signup and view all the answers

    Which type of personal protective equipment (PPE) is specifically required when dealing with highly infectious airborne pathogens?

    <p>N95 respirator (C)</p> Signup and view all the answers

    What is the correct sequence of steps for removing PPE after leaving an isolation room, assuming a gown is worn?

    <p>Gloves, goggles, gown, mask, hands (D)</p> Signup and view all the answers

    What is the purpose of using a High-Efficiency Particulate Air (HEPA) filter?

    <p>To remove particulate matter from the air (A)</p> Signup and view all the answers

    In addition to wearing gloves, what is the first step when entering an isolation room where the use of a gown is indicated?

    <p>Perform hand hygiene (B)</p> Signup and view all the answers

    Which of the following is NOT a typical control measure for infection control

    <p>Turning off the lights (C)</p> Signup and view all the answers

    Flashcards

    Flagella

    Protein filaments used by bacteria for motility.

    Cocci

    Spherical-shaped bacteria that can form clusters or chains.

    Bacilli

    Rod-like shaped bacteria that can be single or chain-like.

    Pili

    Hair-like appendages that help bacteria adhere to surfaces.

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    Endospores

    Dormant, durable forms of bacteria that resist harsh conditions.

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    Botulinum

    A bacterium causing botulism, among the most resistant.

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    Lipopolysaccharide

    Component of Gram-negative bacterial outer membranes; acts as a virulence factor.

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    Capsules

    Polysaccharide layers that protect bacteria from immune responses.

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    Microbiome

    The total genetic material of all microbes within or on the human body.

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    Biofilm

    A group of microbial cells sticking to a surface, surrounded by a polysaccharide matrix.

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    Gram-Positive Cocci (GPC)

    Bacteria that end in '-coccus', including Streptococcus, Staphylococcus, and Enterococcus.

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    Gram-Negative Cocci (GNC)

    Bacteria including Neisseria and Moraxella; only two species in this group.

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    Gram-Positive Bacilli (GPR)

    Bacteria that are rod-shaped excluding Gram-negative bacilli.

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    Prokaryotes vs Eukaryotes

    Prokaryotes have no true nucleus; eukaryotes have a true nucleus with a nuclear membrane.

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    Taxonomic Classification

    System for naming and organizing organisms, starting from Domain to Species.

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    Genus Name

    First name in scientific nomenclature, capitalized and either underlined or italicized.

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    Species Name

    Second name in scientific nomenclature, not capitalized and either underlined or italicized.

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    Strain Definition

    A genetic variant or subtype of a species, not italicized or underlined.

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    Gram Stain Procedure

    A method to classify bacteria into Gram-positive or Gram-negative based on cell wall properties and staining characteristics.

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    Gram-positive Bacteria

    Bacteria with a thick peptidoglycan layer that stains purple during the Gram stain procedure.

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    Gram-negative Bacteria

    Bacteria with a thin peptidoglycan layer and an outer membrane that stains pink/red after the Gram stain procedure.

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    Crystal Violet

    The primary stain used in the Gram stain process, which has a positive charge that binds to negatively charged bacteria.

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    Iodine

    A mordant that interacts with crystal violet to form a complex that helps retain the dye in Gram-positive bacteria.

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    Alcohol Decolorization

    Step in the Gram stain where alcohol removes the crystal violet stain from Gram-negative bacteria due to their thin peptidoglycan layer.

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    Safranin

    A counterstain added after decolorization to visualize Gram-negative bacteria, which become pink/red at this stage.

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    Cell Wall Composition

    The structural differences between Gram-positive (thick peptidoglycan) and Gram-negative (thin peptidoglycan and outer membrane) bacteria.

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    Autoclave

    A device that uses steam under pressure for sterilization.

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    Pasteurization

    A process that heats liquids to kill harmful microbes.

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    Flame sterilization

    Using fire to eliminate bacteria on instruments.

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    UV light

    A type of radiation used to disinfect surfaces in labs.

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    Soap

    Detergent used to remove germs and organic materials.

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    PPE

    Personal protective equipment used to prevent infection.

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    Glove removal

    The proper way to take off gloves to avoid contamination.

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    Hand hygiene

    The practice of cleaning hands to prevent infection.

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    Saprophytes

    Low virulence organisms found in soil, water, and dust, can contaminate labs.

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    Clostridium spp.

    Anaerobic, large, box-car Gram-positive rods inhabiting soil and intestines.

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    Bacillus anthracis

    Highly virulent bacterium causing anthrax in humans and animals.

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    Bacillus cereus

    Bacterium causing food poisoning from rice dishes due to spores surviving cooking.

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    Clostridium perfringens

    Common cause of food poisoning; also causes gas gangrene after wounds or surgeries.

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    Clostridioides difficile

    Bacteria that can overgrow and cause pseudomembranous colitis after antibiotics.

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    Clostridium tetani

    Causes tetanus through neurotoxins, leading to muscle spasms.

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    Clostridium botulinum

    Bacterium that causes food botulism via neurotoxin ingestion from improperly processed foods.

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    Study Notes

    Gram Stain List

    • Gram-Positive Cocci (GPC) are bacteria ending in "-coccus"
      • Streptococcus
      • Staphylococcus
      • Enterococcus
    • Gram-Negative Cocci (GNC) are
      • Neisseria
      • Moraxella
      • (Few species)
    • Gram-Positive Bacilli (GPR): Other bacteria
    • No gram-negative bacilli on Exam 1

    Characteristics of Bacteria (Prokaryotes and Eukaryotes)

    • Prokaryotes: No true nucleus
      • Nucleoid is a single chromosome of double-stranded DNA
      • DNA is in a circular chromosome without a surrounding nuclear membrane
      • No nuclear membrane
      • Cell wall; protein and peptidoglycan; rigid
      • Asexual division
    • Eukaryotes: True nucleus
      • Nucleus contains double-stranded DNA
      • Nuclear bound membrane
      • Most do not have cell walls
      • Sexual and asexual reproduction

    Characteristics of Bacteria (Taxonomy)

    • Domain → Bacteria
    • Phylum → Firmicutes
    • Class → Bacilli
    • Order → Bacillales
    • Family → Staphylococcaceae
    • Genus → Staphylococcus
    • Species → aureus.
    • Genus is capitalized, underlined/italicized
    • Species is lowercase, underlined/italicized
    • Strain is not italicized / underlined

    Characteristics of Bacteria (Gram Stain Procedure)

    • Gram stain is the most commonly used stain in clinical microbiology
    • Classified into Gram Positive and Gram Negative based on the colour they stain
    • Different wall structures lead to different staining outcomes
      • Gram-positive bacteria have a thick peptidoglycan layer
      • Gram-negative bacteria have a thin peptidoglycan layer, and an outer membrane

    Characteristics of Bacteria (Bacterial Replication)

    • Replication is done by binary fission
    • Generation time/doubling time is the time for one cell to divide into two cells

    Characteristics of Bacteria (Bacterial Structure):

    • Cocci = spherical
      • Coccus (round)
      • Streptococci (chain)
      • Diplococci (pair)
    • Bacilli = rod-like
    • Pleomorphic = varied in shape
    • Spirochete = helical (spiral)
    • Flagella = protein filaments for exterior motility
    • Pili = hair-like protein appendages for adherence; don't provide motility
    • Capsule = protective layer
    • DNA in nucleoid region

    Characteristics of Bacteria (Bacterial Structures Continued)

    • Endospores: Dormant, highly durable dehydrated cells with thick walls; resistant to heat, drying, and chemicals
    • Gram-positive bacteria form endospores
    • Important in food industry; survive processing
    • Lipopolysaccharides: essential component of the outer membranes of Gram-negative bacteria
    • Important for virulence factors (fever, shock, attachment site, barrier)
    • Capsule: Organized polysaccharide layers; shields bacteria from immune and phagocytic responses. Important virulence factor

    Characteristics of Bacteria (Microbial Growth Factors)

    • Temperature:
      • Psychrophiles: cold (4-15°C)
      • Mesophiles: moderate (30-35°C)
      • Thermophiles: hot (50-60°C+)
    • Osmotic Pressure
      • Isotonic: equal solute concentration inside and outside the cell
      • Hypotonic: lower solute concentration outside the cell compared to inside
      • Hypertonic: higher solute concentration outside the cell compared to inside
    • Chemical Requirements: Carbon, water, nitrogen, sulfur, phosphorus, trace elements (iron, copper, zinc, etc.)
    • Hydrogen Ion Concentration (pH): medically important bacteria prefer pH 6.0-8.0 (body pH is ~7).
    • Atmosphere:
      • Obligate Aerobes: require oxygen
      • Obligate Anaerobes: don't grow in the presence of oxygen
      • Facultative anaerobes: grow with or without oxygen
      • Capnophilic: grows better with increased CO2

    Host-Parasite Interactions and Terms

    • Host: organism being infected
    • Parasite: any invader or agent
    • Pathogen: microorganism that causes infection
    • Primary pathogen: regularly cause infections
    • Opportunistic pathogen: cause disease only when defenses are weakened
    • Host barriers:
      • Microbiome
      • Anatomical barriers (skin)
      • Stomach acid
      • White blood cells
      • Antibodies

    Host-Parasite Interactions and Terms (Continued)

    • Host factors → disease: behavior, occupation, age, sex, race
    • Organism factors → disease: Evasion of immune response, ability to attach, exotoxins/endotxins, enterotoxins
    • Bacteremia: bacteria multiplying in the bloodstream
    • Virulence: ability of an organism to cause disease
    • Pyogenic: pus producing
    • Disease Transmission:
      • Endogenous (infected with own bacteria)
      • Exogenous (outside bacteria)
        • Contact, droplets/aerosol

    Hospital Epidemiology

    • HAI: Healthcare-associated infections acquired during treatment
    • Colonization: presence of microorganisms on a body surface without disease
    • Infection: organisms invading body tissues
    • Nosocomial infection: acquired by a patient after admission to a hospital
    • Steps in an outbreak:
      • Identify the etiologic agent and conditions
      • Eliminate the reservoir
      • Prevent transmission
      • Prevent future outbreaks

    Sterilization & Disinfection

    • Approaches to control: body's defense mechanisms, chemotherapeutic agents, public health measures, sterilization & disinfection
    • Antiseptic: Inhibits microorganism growth (e.g., alcohol, hydrogen peroxide) applied to skin;reduces number of normal flora organisms
    • Aseptic: used to kill microorganisms (e.g., povidone-iodine) used in procedures such as surgery
    • Cide/Cidal: to kill
    • Static/Stasis: to prevent multiplication without necessarily killing (e.g., preventing bacterial reproduction within a culture)
    • Disinfectant : stronger agents used for surfaces and equipment, possibly too strong for skin

    Sterilization & Disinfection (Hospital/Laboratory control)

    • Hospital:
      • Surgery (cleaning patient's skin, instruments)
      • Medical waste
      • Handwashing
      • PPE
      • Needle stick injury procedures (back bleed, wash wounds)
    • Laboratory:
      • Universal/standard precautions
      • Lab specimens handled as infectious
      • No pipetting by mouth
      • Proper specimen handling

    Sterilization & Disinfection (Methods)

    • Heat: Moist or dry heat, autoclave, pasteurization, flame
    • Filters: Membrane filter, HEPA
    • Radiation: UV light (lab hoods), ionization radiation
    • Soap: removing organic matter/loose skin cells; harbor infectious bacteria

    Sterilization & Disinfection (PPE)

    • PPE for controlling transmission of highly contagious diseases: gloves, lab coat, surgical mask, N95 respirator
    • PPE to prevent transmission of airborne pathogens-N95 respirator, gown, gloves
    • Removal protocol

    Bacteria (Specific)

    • Staphylococcus
      • Gram-positive cocci in grape-like clusters
      • Normal flora of skin, mucous membranes, feces
      • Catalase positive
      • Coagulase: enzyme that binds plasma fibrinogen to form a clot
      • Staph aureus is coagulase positive
      • Other Staph species are negative

    Bacteria (Specific, continued)

    • Staphylococcus aureus
      • Golden colored colony on agar
      • Often found on skin, nose, armpits, and groin
      • Most virulent
      • Virulence factors: coagulase and protein A (prevent phagocytosis), enterotoxins, lipase, hyaluronidase, hemolysins.

    Bacteria (Specific, continued)

    • Staph aureus Disease Associations
      • Skin Infections (impetigo, furuncles, carbuncles, pyogenic abscess)
      • Scalded skin syndrome (blisters/erythema)
      • Food Poisoning (meat and milk products, toxin-mediated)
      • Toxic Shock Syndrome (associated with menstruating females and tampon use)
      • Causes: bacteremia, endocarditis, pneumonia, empyema, osteomyelitis, septic arthritis, gastroenteritis

    Bacteria (Specific, continued)

    • Methicillin Resistant S. aureus (MRSA)
      • Antibiotic resistance
      • Related to acquisition of mec A or mec C gene
      • Resistant to B-lactams
      • Vancomycin, Daptomycin, Tigecycline/ Linezolid alternatives

    Bacteria (Specific, continued)

    • Streptococcus
      • Gram-positive cocci in chains.
      • Normal flora of upper and lower respiratory & intestinal tracts
      • Catalase negative

    Bacteria (Specific, continued)

    • Enterococcus
      • Gram-positive cocci
      • "Streptococci of fecal origin"
      • Normal intestinal flora
      • Relatively low virulence
      • Causes UTIs, endocarditis, wound infections

    Bacteria (Specific, continued)

    • Classification by Hemolysis:
      • Streptococcus are classified by how they breakdown red blood cells (hemolysis)
        • Alpha (partially lysed)
        • Beta (completely lysed)
        • Gamma (not lysed)
    • Streptococcal Diseases:
      • S. pyogenes (Group A): Pharyngitis, scarlet fever, impetigo, sequelae → Rheumatic fever, glomerulonephritis, cellulitis
      • S. agalactiae (Group B): Neonatal Infections (meningitis, septicemia, pneumonia), normal flora in woman's lower genital tract (UTI, bacteremia, in elderly and immunocompromised)
      • S. pneumonia, Viridans strep, strep group D - can cause various other illnesses

    Bacteria (Specific, continued)

    • Antibiotic Treatment of Strep and Enterococcus:
      • Streptococcus: Penicillin=drug of choice, but alternatives include Vancomycin or 3rd-generation cephalosporins
      • Enterococcus: Resistant to penicillins, Cephalosporins, and other B lactams. Requires a combination therapy with penicillins and aminoglycosides, and alternatives such as Vancomycin, Synercid, or Linezolid.

    Bacteria (Specific, continued)

    • Gram-Positive Bacilli
      • Spore-formers:
        • Bacillus spp.: low virulence saprophytes in soil, water, dust, animals; common lab contaminants; large box-car rods
        • Clostridium spp.: inhabit soil, water, vegetation, and the large bowel of humans and other animals; anaerobes; large box-car GPRs
      • Opportunistic Pathogens:
        • Corynebacterium spp.: normal flora of skin/mucous membranes of respiratory and urogenital tract
        • Gardnerella vaginalis
        • Gardnerella vaginalis,
      • Strict Pathogens:
        • Listeria monocytogenes: Stillbirths & meningitis, infect neonates, pregnant women with foodborne illness
        • Corynebacterium diphtheriae: Diphtheria (throat infection)
        • Nocardia spp
        • Bacillus anthracis
        • Clostridium botulinum

    Bacteria (Specific, continued)

    • Non-Spore-Forming GPRs - Characteristics:
      • Corynebacterium spp.: Found in the environment/flora of humans & animals
        • Normal on skin, oral cavity, and genitourinary tract
        • Pleomorphic rods.
      • Nocardia spp.: found in environment but NOT normal flora
        • Partially acid-fast
        • Affects lungs, brain, and skin

    Bacteria (Specific, continued)

    • Gram-Positive Bacilli - Common Diseases:
      • Bacillus anthracis, Bacillus cereus, Clostridium perfringens, Clostridium difficile, Clostridium tetani, Clostridium botulinum

    Bacteria (Specific, continued)

    • Neisseria
      • Two primary pathogens
        • N. gonorrhoeae: Gram-negative diplococci, pili for attachment to epithelial cells, Normal in nasopharynx but can cause gonorrhea.
        • N. meningitidis: Gram-negative diplococci, pili for attachment to epithelial cells, lives in upper respiratory tract but can cause life threatening infections like meningitis and other diseases

    Bacteria (Specific, continued)

    • Moraxella catarrhalis:
      • Gram-negative diplococci
      • Normal flora of upper respiratory tract
      • Not fastidious
      • Otitis media, sinusitis pneumonia, bronchitis

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