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Questions and Answers
During which phase of bacterial growth are the number of cells dividing and dying approximately equal?
During which phase of bacterial growth are the number of cells dividing and dying approximately equal?
Which bacterial structure is primarily associated with adherence to surfaces rather than motility?
Which bacterial structure is primarily associated with adherence to surfaces rather than motility?
What is the primary characteristic of bacteria described as pleomorphic?
What is the primary characteristic of bacteria described as pleomorphic?
Which of the following is a key trait of endospores that allows them to survive adverse conditions?
Which of the following is a key trait of endospores that allows them to survive adverse conditions?
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Which bacterial morphology is characterized by a rod-like shape?
Which bacterial morphology is characterized by a rod-like shape?
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Which bacterial structure is known for its role in shielding the bacteria from immune responses?
Which bacterial structure is known for its role in shielding the bacteria from immune responses?
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What is the primary function of lipopolysaccharide in Gram-negative bacteria?
What is the primary function of lipopolysaccharide in Gram-negative bacteria?
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Which term describes the genetic material of all microbes living in and on the human body?
Which term describes the genetic material of all microbes living in and on the human body?
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What characteristic of a biofilm is critical for its formation and function?
What characteristic of a biofilm is critical for its formation and function?
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What is the most significant difference between microbiota and microbiome?
What is the most significant difference between microbiota and microbiome?
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Which of the following best describes the cellular structure of prokaryotes?
Which of the following best describes the cellular structure of prokaryotes?
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Which of these microorganisms is classified as a Gram-negative cocci?
Which of these microorganisms is classified as a Gram-negative cocci?
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In scientific nomenclature, which of the following is the correct way to write the species name?
In scientific nomenclature, which of the following is the correct way to write the species name?
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Which of the following is a defining characteristic of eukaryotic cells?
Which of the following is a defining characteristic of eukaryotic cells?
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Which of the following describes the cell wall of prokaryotic cells?
Which of the following describes the cell wall of prokaryotic cells?
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Which of the following is a correct descending order of taxonomic classification?
Which of the following is a correct descending order of taxonomic classification?
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Which of the following is NOT a Gram-positive cocci?
Which of the following is NOT a Gram-positive cocci?
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What is the term for a genetic variant or subtype of a microorganism, such as E. coli O157:H7?
What is the term for a genetic variant or subtype of a microorganism, such as E. coli O157:H7?
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What is the primary purpose of the Gram stain in clinical microbiology?
What is the primary purpose of the Gram stain in clinical microbiology?
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Which component of Gram-positive bacteria contributes to their purple staining after the Gram stain procedure?
Which component of Gram-positive bacteria contributes to their purple staining after the Gram stain procedure?
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What is the role of iodine in the Gram staining process?
What is the role of iodine in the Gram staining process?
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What is the function of the alcohol during the Gram stain procedure?
What is the function of the alcohol during the Gram stain procedure?
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Which reagent is used as a counterstain in the Gram stain?
Which reagent is used as a counterstain in the Gram stain?
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What is the expected color of Gram-negative bacteria after the complete Gram stain procedure?
What is the expected color of Gram-negative bacteria after the complete Gram stain procedure?
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Why do acid-fast bacteria not stain well with the Gram stain method?
Why do acid-fast bacteria not stain well with the Gram stain method?
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Which bacterial cell structure is directly responsible for the differential staining observed in Gram-positive and Gram-negative bacteria?
Which bacterial cell structure is directly responsible for the differential staining observed in Gram-positive and Gram-negative bacteria?
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Which characteristic is LEAST associated with the Bacillus genus?
Which characteristic is LEAST associated with the Bacillus genus?
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What is a primary concern with Clostridium perfringens infections?
What is a primary concern with Clostridium perfringens infections?
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Which Clostridium species is MOST likely to overgrow following prolonged antibiotic usage?
Which Clostridium species is MOST likely to overgrow following prolonged antibiotic usage?
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Which symptom is MOST specifically associated with Clostridium tetani?
Which symptom is MOST specifically associated with Clostridium tetani?
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Which of these options is a key factor that contributes to food botulism?
Which of these options is a key factor that contributes to food botulism?
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What is a defining Gram staining characteristic for both Bacillus and Clostridium species?
What is a defining Gram staining characteristic for both Bacillus and Clostridium species?
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What is a common method of transmission for Bacillus cereus food poisoning?
What is a common method of transmission for Bacillus cereus food poisoning?
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Which of these options is an element of treatment MOST specific to Clostridium tetani infection?
Which of these options is an element of treatment MOST specific to Clostridium tetani infection?
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What is the primary function of soap in hand hygiene when disinfecting a surface or hands?
What is the primary function of soap in hand hygiene when disinfecting a surface or hands?
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Which of the following methods can achieve sterilization?
Which of the following methods can achieve sterilization?
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When performing hand hygiene, what is the first step according to the instructions?
When performing hand hygiene, what is the first step according to the instructions?
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Which type of personal protective equipment (PPE) is specifically required when dealing with highly infectious airborne pathogens?
Which type of personal protective equipment (PPE) is specifically required when dealing with highly infectious airborne pathogens?
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What is the correct sequence of steps for removing PPE after leaving an isolation room, assuming a gown is worn?
What is the correct sequence of steps for removing PPE after leaving an isolation room, assuming a gown is worn?
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What is the purpose of using a High-Efficiency Particulate Air (HEPA) filter?
What is the purpose of using a High-Efficiency Particulate Air (HEPA) filter?
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In addition to wearing gloves, what is the first step when entering an isolation room where the use of a gown is indicated?
In addition to wearing gloves, what is the first step when entering an isolation room where the use of a gown is indicated?
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Which of the following is NOT a typical control measure for infection control
Which of the following is NOT a typical control measure for infection control
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Flashcards
Flagella
Flagella
Protein filaments used by bacteria for motility.
Cocci
Cocci
Spherical-shaped bacteria that can form clusters or chains.
Bacilli
Bacilli
Rod-like shaped bacteria that can be single or chain-like.
Pili
Pili
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Endospores
Endospores
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Botulinum
Botulinum
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Lipopolysaccharide
Lipopolysaccharide
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Capsules
Capsules
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Microbiome
Microbiome
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Biofilm
Biofilm
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Gram-Positive Cocci (GPC)
Gram-Positive Cocci (GPC)
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Gram-Negative Cocci (GNC)
Gram-Negative Cocci (GNC)
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Gram-Positive Bacilli (GPR)
Gram-Positive Bacilli (GPR)
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Prokaryotes vs Eukaryotes
Prokaryotes vs Eukaryotes
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Taxonomic Classification
Taxonomic Classification
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Genus Name
Genus Name
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Species Name
Species Name
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Strain Definition
Strain Definition
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Gram Stain Procedure
Gram Stain Procedure
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Gram-positive Bacteria
Gram-positive Bacteria
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Gram-negative Bacteria
Gram-negative Bacteria
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Crystal Violet
Crystal Violet
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Iodine
Iodine
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Alcohol Decolorization
Alcohol Decolorization
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Safranin
Safranin
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Cell Wall Composition
Cell Wall Composition
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Autoclave
Autoclave
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Pasteurization
Pasteurization
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Flame sterilization
Flame sterilization
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UV light
UV light
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Soap
Soap
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PPE
PPE
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Glove removal
Glove removal
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Hand hygiene
Hand hygiene
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Saprophytes
Saprophytes
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Clostridium spp.
Clostridium spp.
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Bacillus anthracis
Bacillus anthracis
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Bacillus cereus
Bacillus cereus
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Clostridium perfringens
Clostridium perfringens
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Clostridioides difficile
Clostridioides difficile
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Clostridium tetani
Clostridium tetani
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Clostridium botulinum
Clostridium botulinum
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Study Notes
Gram Stain List
- Gram-Positive Cocci (GPC) are bacteria ending in "-coccus"
- Streptococcus
- Staphylococcus
- Enterococcus
- Gram-Negative Cocci (GNC) are
- Neisseria
- Moraxella
- (Few species)
- Gram-Positive Bacilli (GPR): Other bacteria
- No gram-negative bacilli on Exam 1
Characteristics of Bacteria (Prokaryotes and Eukaryotes)
- Prokaryotes: No true nucleus
- Nucleoid is a single chromosome of double-stranded DNA
- DNA is in a circular chromosome without a surrounding nuclear membrane
- No nuclear membrane
- Cell wall; protein and peptidoglycan; rigid
- Asexual division
- Eukaryotes: True nucleus
- Nucleus contains double-stranded DNA
- Nuclear bound membrane
- Most do not have cell walls
- Sexual and asexual reproduction
Characteristics of Bacteria (Taxonomy)
- Domain → Bacteria
- Phylum → Firmicutes
- Class → Bacilli
- Order → Bacillales
- Family → Staphylococcaceae
- Genus → Staphylococcus
- Species → aureus.
- Genus is capitalized, underlined/italicized
- Species is lowercase, underlined/italicized
- Strain is not italicized / underlined
Characteristics of Bacteria (Gram Stain Procedure)
- Gram stain is the most commonly used stain in clinical microbiology
- Classified into Gram Positive and Gram Negative based on the colour they stain
- Different wall structures lead to different staining outcomes
- Gram-positive bacteria have a thick peptidoglycan layer
- Gram-negative bacteria have a thin peptidoglycan layer, and an outer membrane
Characteristics of Bacteria (Bacterial Replication)
- Replication is done by binary fission
- Generation time/doubling time is the time for one cell to divide into two cells
Characteristics of Bacteria (Bacterial Structure):
- Cocci = spherical
- Coccus (round)
- Streptococci (chain)
- Diplococci (pair)
- Bacilli = rod-like
- Pleomorphic = varied in shape
- Spirochete = helical (spiral)
- Flagella = protein filaments for exterior motility
- Pili = hair-like protein appendages for adherence; don't provide motility
- Capsule = protective layer
- DNA in nucleoid region
Characteristics of Bacteria (Bacterial Structures Continued)
- Endospores: Dormant, highly durable dehydrated cells with thick walls; resistant to heat, drying, and chemicals
- Gram-positive bacteria form endospores
- Important in food industry; survive processing
- Lipopolysaccharides: essential component of the outer membranes of Gram-negative bacteria
- Important for virulence factors (fever, shock, attachment site, barrier)
- Capsule: Organized polysaccharide layers; shields bacteria from immune and phagocytic responses. Important virulence factor
Characteristics of Bacteria (Microbial Growth Factors)
- Temperature:
- Psychrophiles: cold (4-15°C)
- Mesophiles: moderate (30-35°C)
- Thermophiles: hot (50-60°C+)
- Osmotic Pressure
- Isotonic: equal solute concentration inside and outside the cell
- Hypotonic: lower solute concentration outside the cell compared to inside
- Hypertonic: higher solute concentration outside the cell compared to inside
- Chemical Requirements: Carbon, water, nitrogen, sulfur, phosphorus, trace elements (iron, copper, zinc, etc.)
- Hydrogen Ion Concentration (pH): medically important bacteria prefer pH 6.0-8.0 (body pH is ~7).
- Atmosphere:
- Obligate Aerobes: require oxygen
- Obligate Anaerobes: don't grow in the presence of oxygen
- Facultative anaerobes: grow with or without oxygen
- Capnophilic: grows better with increased CO2
Host-Parasite Interactions and Terms
- Host: organism being infected
- Parasite: any invader or agent
- Pathogen: microorganism that causes infection
- Primary pathogen: regularly cause infections
- Opportunistic pathogen: cause disease only when defenses are weakened
- Host barriers:
- Microbiome
- Anatomical barriers (skin)
- Stomach acid
- White blood cells
- Antibodies
Host-Parasite Interactions and Terms (Continued)
- Host factors → disease: behavior, occupation, age, sex, race
- Organism factors → disease: Evasion of immune response, ability to attach, exotoxins/endotxins, enterotoxins
- Bacteremia: bacteria multiplying in the bloodstream
- Virulence: ability of an organism to cause disease
- Pyogenic: pus producing
- Disease Transmission:
- Endogenous (infected with own bacteria)
- Exogenous (outside bacteria)
- Contact, droplets/aerosol
Hospital Epidemiology
- HAI: Healthcare-associated infections acquired during treatment
- Colonization: presence of microorganisms on a body surface without disease
- Infection: organisms invading body tissues
- Nosocomial infection: acquired by a patient after admission to a hospital
- Steps in an outbreak:
- Identify the etiologic agent and conditions
- Eliminate the reservoir
- Prevent transmission
- Prevent future outbreaks
Sterilization & Disinfection
- Approaches to control: body's defense mechanisms, chemotherapeutic agents, public health measures, sterilization & disinfection
- Antiseptic: Inhibits microorganism growth (e.g., alcohol, hydrogen peroxide) applied to skin;reduces number of normal flora organisms
- Aseptic: used to kill microorganisms (e.g., povidone-iodine) used in procedures such as surgery
- Cide/Cidal: to kill
- Static/Stasis: to prevent multiplication without necessarily killing (e.g., preventing bacterial reproduction within a culture)
- Disinfectant : stronger agents used for surfaces and equipment, possibly too strong for skin
Sterilization & Disinfection (Hospital/Laboratory control)
- Hospital:
- Surgery (cleaning patient's skin, instruments)
- Medical waste
- Handwashing
- PPE
- Needle stick injury procedures (back bleed, wash wounds)
- Laboratory:
- Universal/standard precautions
- Lab specimens handled as infectious
- No pipetting by mouth
- Proper specimen handling
Sterilization & Disinfection (Methods)
- Heat: Moist or dry heat, autoclave, pasteurization, flame
- Filters: Membrane filter, HEPA
- Radiation: UV light (lab hoods), ionization radiation
- Soap: removing organic matter/loose skin cells; harbor infectious bacteria
Sterilization & Disinfection (PPE)
- PPE for controlling transmission of highly contagious diseases: gloves, lab coat, surgical mask, N95 respirator
- PPE to prevent transmission of airborne pathogens-N95 respirator, gown, gloves
- Removal protocol
Bacteria (Specific)
- Staphylococcus
- Gram-positive cocci in grape-like clusters
- Normal flora of skin, mucous membranes, feces
- Catalase positive
- Coagulase: enzyme that binds plasma fibrinogen to form a clot
- Staph aureus is coagulase positive
- Other Staph species are negative
Bacteria (Specific, continued)
- Staphylococcus aureus
- Golden colored colony on agar
- Often found on skin, nose, armpits, and groin
- Most virulent
- Virulence factors: coagulase and protein A (prevent phagocytosis), enterotoxins, lipase, hyaluronidase, hemolysins.
Bacteria (Specific, continued)
- Staph aureus Disease Associations
- Skin Infections (impetigo, furuncles, carbuncles, pyogenic abscess)
- Scalded skin syndrome (blisters/erythema)
- Food Poisoning (meat and milk products, toxin-mediated)
- Toxic Shock Syndrome (associated with menstruating females and tampon use)
- Causes: bacteremia, endocarditis, pneumonia, empyema, osteomyelitis, septic arthritis, gastroenteritis
Bacteria (Specific, continued)
- Methicillin Resistant S. aureus (MRSA)
- Antibiotic resistance
- Related to acquisition of mec A or mec C gene
- Resistant to B-lactams
- Vancomycin, Daptomycin, Tigecycline/ Linezolid alternatives
Bacteria (Specific, continued)
- Streptococcus
- Gram-positive cocci in chains.
- Normal flora of upper and lower respiratory & intestinal tracts
- Catalase negative
Bacteria (Specific, continued)
- Enterococcus
- Gram-positive cocci
- "Streptococci of fecal origin"
- Normal intestinal flora
- Relatively low virulence
- Causes UTIs, endocarditis, wound infections
Bacteria (Specific, continued)
- Classification by Hemolysis:
- Streptococcus are classified by how they breakdown red blood cells (hemolysis)
- Alpha (partially lysed)
- Beta (completely lysed)
- Gamma (not lysed)
- Streptococcus are classified by how they breakdown red blood cells (hemolysis)
- Streptococcal Diseases:
- S. pyogenes (Group A): Pharyngitis, scarlet fever, impetigo, sequelae → Rheumatic fever, glomerulonephritis, cellulitis
- S. agalactiae (Group B): Neonatal Infections (meningitis, septicemia, pneumonia), normal flora in woman's lower genital tract (UTI, bacteremia, in elderly and immunocompromised)
- S. pneumonia, Viridans strep, strep group D - can cause various other illnesses
Bacteria (Specific, continued)
- Antibiotic Treatment of Strep and Enterococcus:
- Streptococcus: Penicillin=drug of choice, but alternatives include Vancomycin or 3rd-generation cephalosporins
- Enterococcus: Resistant to penicillins, Cephalosporins, and other B lactams. Requires a combination therapy with penicillins and aminoglycosides, and alternatives such as Vancomycin, Synercid, or Linezolid.
Bacteria (Specific, continued)
- Gram-Positive Bacilli
- Spore-formers:
- Bacillus spp.: low virulence saprophytes in soil, water, dust, animals; common lab contaminants; large box-car rods
- Clostridium spp.: inhabit soil, water, vegetation, and the large bowel of humans and other animals; anaerobes; large box-car GPRs
- Opportunistic Pathogens:
- Corynebacterium spp.: normal flora of skin/mucous membranes of respiratory and urogenital tract
- Gardnerella vaginalis
- Gardnerella vaginalis,
- Strict Pathogens:
- Listeria monocytogenes: Stillbirths & meningitis, infect neonates, pregnant women with foodborne illness
- Corynebacterium diphtheriae: Diphtheria (throat infection)
- Nocardia spp
- Bacillus anthracis
- Clostridium botulinum
- Spore-formers:
Bacteria (Specific, continued)
- Non-Spore-Forming GPRs - Characteristics:
- Corynebacterium spp.: Found in the environment/flora of humans & animals
- Normal on skin, oral cavity, and genitourinary tract
- Pleomorphic rods.
- Nocardia spp.: found in environment but NOT normal flora
- Partially acid-fast
- Affects lungs, brain, and skin
- Corynebacterium spp.: Found in the environment/flora of humans & animals
Bacteria (Specific, continued)
- Gram-Positive Bacilli - Common Diseases:
- Bacillus anthracis, Bacillus cereus, Clostridium perfringens, Clostridium difficile, Clostridium tetani, Clostridium botulinum
Bacteria (Specific, continued)
- Neisseria
- Two primary pathogens
- N. gonorrhoeae: Gram-negative diplococci, pili for attachment to epithelial cells, Normal in nasopharynx but can cause gonorrhea.
- N. meningitidis: Gram-negative diplococci, pili for attachment to epithelial cells, lives in upper respiratory tract but can cause life threatening infections like meningitis and other diseases
- Two primary pathogens
Bacteria (Specific, continued)
- Moraxella catarrhalis:
- Gram-negative diplococci
- Normal flora of upper respiratory tract
- Not fastidious
- Otitis media, sinusitis pneumonia, bronchitis
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Description
Test your knowledge of fundamental microbiology concepts, including bacterial growth phases, structures, and characteristics. This quiz covers key traits of bacteria, their interactions with the environment, and comparisons between microbiota and microbiome. Perfect for anyone studying microbiology or related fields!