Podcast
Questions and Answers
Which bacterial component is specifically targeted by penicillin antibiotics?
Which bacterial component is specifically targeted by penicillin antibiotics?
- Ribosomes
- DNA
- Peptidoglycan (correct)
- Cell membrane
Which of the following mechanisms enables bacteria to resist macrolide antibiotics?
Which of the following mechanisms enables bacteria to resist macrolide antibiotics?
- Methylating the 50S ribosomal subunit (correct)
- Modifying the antibiotic molecule
- Producing enzymes that degrade the antibiotic
- Altering the bacterial cell wall to prevent entry
Which of these best describes the function of bacterial ribosomes?
Which of these best describes the function of bacterial ribosomes?
- Cell motility
- Protein synthesis (correct)
- Energy production
- DNA replication
What role do lipopolysaccharides (LPS) play in Gram-negative bacteria?
What role do lipopolysaccharides (LPS) play in Gram-negative bacteria?
Which feature differentiates Gram-positive from Gram-negative bacteria?
Which feature differentiates Gram-positive from Gram-negative bacteria?
What is the primary purpose of a Gram stain in microbiology?
What is the primary purpose of a Gram stain in microbiology?
Which of these factors increases the risk of infection?
Which of these factors increases the risk of infection?
How do bacteria acquire antibiotic resistance through horizontal gene transfer involving a pilus?
How do bacteria acquire antibiotic resistance through horizontal gene transfer involving a pilus?
What role does the enzyme beta-lactamase play in antibiotic resistance?
What role does the enzyme beta-lactamase play in antibiotic resistance?
Why are viruses considered obligate intracellular parasites?
Why are viruses considered obligate intracellular parasites?
What is the function of the viral capsid?
What is the function of the viral capsid?
How does viral budding contribute to persistent infections?
How does viral budding contribute to persistent infections?
What is the role of antibodies in adaptive immunity?
What is the role of antibodies in adaptive immunity?
What is the primary goal of vaccination?
What is the primary goal of vaccination?
How do live attenuated vaccines work?
How do live attenuated vaccines work?
What is the difference between general-purpose and enriched media in culturing bacteria?
What is the difference between general-purpose and enriched media in culturing bacteria?
A selective and differential medium is used to distinguish bacteria. What properties must it have?
A selective and differential medium is used to distinguish bacteria. What properties must it have?
What is the main advantage of using nucleic acid amplification testing (NAAT) over traditional culture methods?
What is the main advantage of using nucleic acid amplification testing (NAAT) over traditional culture methods?
What is the role of the mucociliary escalator in the respiratory tract's defense?
What is the role of the mucociliary escalator in the respiratory tract's defense?
Why are immunosuppressed individuals more susceptible to infections?
Why are immunosuppressed individuals more susceptible to infections?
Which route of transmission involves pathogens that are excreted from the gut of an infected person and enter a new host through ingestion?
Which route of transmission involves pathogens that are excreted from the gut of an infected person and enter a new host through ingestion?
Which method of transmission requires a vector to carry the pathogen from one host to another?
Which method of transmission requires a vector to carry the pathogen from one host to another?
In healthcare settings, what is the most common mode of transmission of pathogens?
In healthcare settings, what is the most common mode of transmission of pathogens?
Which of the following is an example of a fomite that can transmit pathogens?
Which of the following is an example of a fomite that can transmit pathogens?
Which public health strategy is most effective in reducing the spread of vaccine-preventable diseases?
Which public health strategy is most effective in reducing the spread of vaccine-preventable diseases?
What is the primary method for preventing the spread of influenza?
What is the primary method for preventing the spread of influenza?
How accurate is it to say that the influenza vaccine provides protection against Haemophilus influenzae?
How accurate is it to say that the influenza vaccine provides protection against Haemophilus influenzae?
Which vaccination is given to women who are pregnant to protect them and their babies?
Which vaccination is given to women who are pregnant to protect them and their babies?
Normal microbiota in the gut becomes pathogenic when?
Normal microbiota in the gut becomes pathogenic when?
Cell-based non-culture systems, nucleic acid-based tests and serological tests can be used for which of the organisms?
Cell-based non-culture systems, nucleic acid-based tests and serological tests can be used for which of the organisms?
Serology can be used for originally tested blood serum but is also good for ?
Serology can be used for originally tested blood serum but is also good for ?
Vaccinations are created by antibodies, B cells, T cells and what other constituent?
Vaccinations are created by antibodies, B cells, T cells and what other constituent?
What kind of organisms can produce infection
What kind of organisms can produce infection
Which type of cell causes high risk of infection?
Which type of cell causes high risk of infection?
Which characteristic is exclusive to Gram-positive bacteria?
Which characteristic is exclusive to Gram-positive bacteria?
What is the primary function of fimbriae on bacterial cells?
What is the primary function of fimbriae on bacterial cells?
Why are endospores clinically significant?
Why are endospores clinically significant?
Which feature distinguishes a bacterial cell from a virus?
Which feature distinguishes a bacterial cell from a virus?
Which best describes the method by which aminoglycosides inhibit bacterial growth?
Which best describes the method by which aminoglycosides inhibit bacterial growth?
How does bacterial conjugation contribute to antibiotic resistance?
How does bacterial conjugation contribute to antibiotic resistance?
Why is it important to identify if an isolated microorganism is a potential pathogen or normal flora?
Why is it important to identify if an isolated microorganism is a potential pathogen or normal flora?
What information does the Gram stain provide?
What information does the Gram stain provide?
In a broth dilution test, how do you interpret the MIC?
In a broth dilution test, how do you interpret the MIC?
Which of the mechanisms enables antibiotic resistance by horizontal gene transfer?
Which of the mechanisms enables antibiotic resistance by horizontal gene transfer?
What is the primary role of the viral capsid?
What is the primary role of the viral capsid?
What is the critical factor that dictates the host range of a virus?
What is the critical factor that dictates the host range of a virus?
Which of the following best describes a persistent viral infection?
Which of the following best describes a persistent viral infection?
During viral replication, what process does the term 'genome copying' describe?
During viral replication, what process does the term 'genome copying' describe?
How does an inactivated vaccine stimulate an adaptive immune response?
How does an inactivated vaccine stimulate an adaptive immune response?
What feature differentiates inactivated vaccines from live attenuated vaccines?
What feature differentiates inactivated vaccines from live attenuated vaccines?
Why are booster doses often required for inactivated vaccines?
Why are booster doses often required for inactivated vaccines?
Which method of bacterial identification relies on observing bacterial growth patterns and characteristics?
Which method of bacterial identification relies on observing bacterial growth patterns and characteristics?
Why is NAAT more advantageous than traditional culture methods?
Why is NAAT more advantageous than traditional culture methods?
A patient presents with a wound infection. The lab performs a Gram stain and reports 'Gram-positive cocci in clusters.' What type of bacteria is most likely?
A patient presents with a wound infection. The lab performs a Gram stain and reports 'Gram-positive cocci in clusters.' What type of bacteria is most likely?
What is a key characteristic of bacteria identified as acid-fast positive?
What is a key characteristic of bacteria identified as acid-fast positive?
An organism that grows equally well in the presence or absence of oxygen is classified as what type of anaerobe?
An organism that grows equally well in the presence or absence of oxygen is classified as what type of anaerobe?
Which of the following best describes the role of lysozymes in host defense?
Which of the following best describes the role of lysozymes in host defense?
The dryness and acidity of the skin contribute to host defense primarily by:
The dryness and acidity of the skin contribute to host defense primarily by:
Which function do macrophages perform in innate immunity?
Which function do macrophages perform in innate immunity?
Exposure to which factor would most directly compromise the effectiveness of the mucociliary escalator in the respiratory tract?
Exposure to which factor would most directly compromise the effectiveness of the mucociliary escalator in the respiratory tract?
Acidity in the stomach functions as a defense mechanism by:
Acidity in the stomach functions as a defense mechanism by:
What is the MOST likely mode of disease transmission for influenza?
What is the MOST likely mode of disease transmission for influenza?
Which situation below illustrates indirect contact transmission?
Which situation below illustrates indirect contact transmission?
In what way does sanitation reduce the spread of infectious diseases?
In what way does sanitation reduce the spread of infectious diseases?
Which type of transmission is typically associated with Clostridium tetani infections?
Which type of transmission is typically associated with Clostridium tetani infections?
In which scenario is the avoidance of air travel most appropriate?
In which scenario is the avoidance of air travel most appropriate?
What is the rationale of routine vaccination programs?
What is the rationale of routine vaccination programs?
What type of information about vaccination might a pharmacist obtain with the Centers for Disease Control and Prevention (CDC) resource?
What type of information about vaccination might a pharmacist obtain with the Centers for Disease Control and Prevention (CDC) resource?
When a patient in his last trimester of pregnancy with influenza asks if he avoid what not eat what is your advice?
When a patient in his last trimester of pregnancy with influenza asks if he avoid what not eat what is your advice?
If for instance a family member needs a Hep A vaxcine who should you not give it do and where does one obtain?
If for instance a family member needs a Hep A vaxcine who should you not give it do and where does one obtain?
After a patient has just had a vaccine what to you suggest at the end of a session?
After a patient has just had a vaccine what to you suggest at the end of a session?
How do bacteria, such as some Gram-negative organisms, prevent drugs like vancomycin from reaching their targets?
How do bacteria, such as some Gram-negative organisms, prevent drugs like vancomycin from reaching their targets?
What is the rationale behind administering a combination vaccine like Tdap?
What is the rationale behind administering a combination vaccine like Tdap?
Which factor primarily determines the likelihood that a microorganism will cause an infection in a host?
Which factor primarily determines the likelihood that a microorganism will cause an infection in a host?
A patient is diagnosed with a latent viral infection. Which statement accurately describes this condition?
A patient is diagnosed with a latent viral infection. Which statement accurately describes this condition?
A researcher is investigating the mechanism of action of a new antiviral drug. The drug prevents viral entry into cells by blocking the interaction between viral surface proteins and host cell receptors. Which step of the viral life cycle is being targeted?
A researcher is investigating the mechanism of action of a new antiviral drug. The drug prevents viral entry into cells by blocking the interaction between viral surface proteins and host cell receptors. Which step of the viral life cycle is being targeted?
Flashcards
Cell Wall Synthesis Inhibitors
Cell Wall Synthesis Inhibitors
Drugs that target and disrupt cell wall formation in bacteria.
Protein Synthesis Inhibitors
Protein Synthesis Inhibitors
Substances that interfere with protein production in bacteria to inhibit growth.
DNA Inhibitors
DNA Inhibitors
Medications that target bacterial DNA to halt replication and cell division.
RNA Inhibitors
RNA Inhibitors
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Membrane Attackers
Membrane Attackers
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Folate Synthesis Inhibitors
Folate Synthesis Inhibitors
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Normal Flora
Normal Flora
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Pathogenic Bacteria
Pathogenic Bacteria
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Bacterial Cell Walls
Bacterial Cell Walls
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Gram-Positive
Gram-Positive
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Identifying Bacteria
Identifying Bacteria
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Gram-Negative
Gram-Negative
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Bacterial Culture
Bacterial Culture
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Aerobes
Aerobes
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Anaerobes
Anaerobes
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Pili
Pili
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Flagella
Flagella
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Cell Wall
Cell Wall
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Gram Stain
Gram Stain
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Minimum Inhibitory Concentration
Minimum Inhibitory Concentration
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Disc-Diffusion
Disc-Diffusion
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Bacterial Conjugation
Bacterial Conjugation
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Obligate Intracellular Parasites
Obligate Intracellular Parasites
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Viral Capsid
Viral Capsid
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Virion
Virion
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Host - Innate Immune Response
Host - Innate Immune Response
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Host - Adaptive Immune Response
Host - Adaptive Immune Response
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Immunisation
Immunisation
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Immunisation - Passive Immunity
Immunisation - Passive Immunity
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Immunisation - Active Immunity
Immunisation - Active Immunity
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Inactivated Vaccine
Inactivated Vaccine
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Live Attenuated Vaccine
Live Attenuated Vaccine
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Vaccine Hesitancy
Vaccine Hesitancy
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Person-to-person transmission
Person-to-person transmission
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Fomites
Fomites
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Zoonoses Transmission
Zoonoses Transmission
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Respiratory droplet.
Respiratory droplet.
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Horizontal Gene Transfer
Horizontal Gene Transfer
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Altered Permeability / Efflux Pumps
Altered Permeability / Efflux Pumps
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Study Notes
Microbial world introduction
- Harmless or beneficial microorganisms reside on and in the body
- Staphylococci on the skin and Escherichia coli in the gut are examples
- Some normal flora turn into opportunistic pathogens
- Can cause disease if they move to different body sites or immunity is low
Bacterial Basics
- Prokaryotic cells contrast human eukaryotic cells
- Small, ranging from 1-10 μm
- Not cells, unlike fungi, yeasts, and viruses
Pathogens
- Pathogenic Bacteria (Disease-Causing) can be acquired from the environment through inhalation, ingestion, or contact
- Bacteria can also be aquired from normal flora if bacteria access new body sites or immunity is weak
Factors Affecting Infection Risk
- Microorganism's ability to cause harm
- Number of microorganisms acquired
- Patient's immune system strength
Cell Structures
- A bacterial cell is prokaryotic and lacks a nucleus.
- Animal cells are eukaryotic with a nucleus
- Bacterial cells have a cell wall made of peptidoglycan, which is absent in animal cells
- Bacterial DNA is a circular chromosome found in the nucleoid region, while animal DNA is linear and enclosed in a nucleus
- Bacteria lack membrane-bound organelles like mitochondria
- Bacteria are smaller than animal cells, have smaller ribosomes (70S), and reproduce asexually through binary fission
- Some bacteria have flagella or pili for movement, while animal cells use cilia or cytoskeleton
Bacteria Cell Wall
- Plasmids, present in bacteria, are absent in animal cells.
- Bacteria can have capsules for protection, while animal cells do not
- Bacteria provide shape, structural support, and resistance to osmotic pressure
Features of Bacteria
- Not all bacteria have a cell wall
- Capsules are protective, adhesion and receptor functions.
- Endospores are dormant forms of bacterium, allowing survival amid adverse conditions
- Fimbriae are hair-like structures involved for adhesion
- Flagella are tail(s) enabling motility
- Inclusion granules store additional material
- Pilus allows adhesion and translocation of proteins and DNA
- Plasmids allows a source of genes
- Outer membrane is part of the cell wall
Gram Positive Bacteria
- Thick peptidoglycan with alternating repeats
- Contains teichoic acid and lipoteichoic acid
Gram Negative Bacteria
- Thin peptidoglycan
- Additional outer membrane which controls the flow of substances
- Contains lipopolysaccharides with endotoxins for stabilization and protection
Identifying Microorganisms: Microscopy
- Stains that visualize
- Provides quick insight into Gram reaction (positive/negative), cell shape, and arrangement
Identifying Microorganisms: Culture
- Samples are taken from the affected site and placed in solid or liquid media
- Grown under ~37°C, varies by pathogen
- Flora through subculture
- Bacteria characteristics through microscopy or growth patterns
Antibiotic Susceptibility
- Determines antibiotics resistant to
Rapid Stage & Microscopy
- Occurs within hours
- Cell shape, arrangement, and Gram reaction
Intermediate Result
- Takes 24-48 hours
- Involves preliminary identification of the microorganism
Final result
- Takes several days
- Provides definitive identification and antibiotic susceptibility
Culture Identification
- Identify if isolated microorganisms are potential pathogens or normal flora
- Normal flora are the expected microorganisms at body locations
- Potential pathogens may induce disease if present
- Culture provides live sample
- Culture is limited by growth in artificial media
Cell Arrangement
- Round spherical (coccus), rod shaped, or curved spirals
- Bacterial specific arrangements aid the identification
- Chains divide in one plane
- Clusters with multiple divide, grapelike
- Some genera are pairs
- Four cell groups in square formation
Gram Stain
- Makes bateria easier to see
- Classifies bacteria based on cell wall composition
- Differential stain for cell characteristics
Gram Positive Properties
- Thick peptidoglycan layer
- Retains purple stain
Gram Negative Properties
- Thin peptidoglycan
- Decolourized by alchohol and stain with pink/red
Atypical Bacteria
- Does not clearly stain
- Contains mycoplasmas which lacks a cell wall
Culture of Microbes
- Grow on artificial nutrient environments Provides nutrients/conditions to specific/select/differentiate growth
- OBLIGATE aerobes require oxygen at tube tops.
- OBLIGATE ANAEROBES cannot with oxygen, grow bottom tube.
- FACULTATIVE ANAEROBES grow tube, oxygen presence
- AEROTOLERANT anaerobes grow with or without,
- MICROAEROPHILES require reduced oxygen, middle tube
Media
- General supports bacteria
- Enriched contains things that supports bacteria
- Selective inhibits some to grow
- Differential is bacteria distinction for identification
Non-Culture Identification
- Useful for hard to culture
Biochemical
- Different pathways aid identification Tests run on strips Positive show color
DNA/RNA
- Bacteria identification
- PCR tests detect SARS_19 or polymerase test
- results 24 compared traditional days
- Can't detect organism susceptibility
Testing Serum
- based antigens -detect pathogens
- Antibodies and antigens
- Multi -well micro plates
Process
- Coat with antigens for prep
- Patent Serum with antibody binds
- Serum bonds with and remotes
- Chlorogenic enzymes
Results
- Development on visual
- Colour light, intensity and concentration
Antimicrobial Agents
- Inhibits bacterial growth
- Low drug controls. High ones work
Resistance
- First of tube with growth on agar
- Tests Simpler
- Antibiotic disc plates create cleaning
Results
- Based on radius of cleaning Suspectible at .06 Resistant is at 2
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