Microbial Secondary Metabolites

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Questions and Answers

What is the primary amino acid involved in the biosynthesis of penicillin?

  • Valine
  • Cysteine
  • Phenylalanine
  • L-α-aminoadipate (correct)

What is the primary reason for adding phenyl-alanine or phenyl acetic acid during penicillin fermentation?

  • To enhance the growth of Penicillium chrysogenum
  • To increase the yield of penicillin
  • To produce only the desired penicillin G (correct)
  • To reduce the production of pigments

Which of the following is NOT a characteristic of Penicillium notatum?

  • Low yield of penicillin
  • High yield of penicillin
  • Production of pigments
  • Non-pigmented (correct)

What is the main advantage of using Penicillium chrysogenum mutants in penicillin production?

<p>Increased yield of penicillin (B)</p> Signup and view all the answers

Which of the following is an example of a secondary metabolite produced by microorganisms?

<p>All of the above (D)</p> Signup and view all the answers

What is the primary function of CaCO3 and KH2PO4 in penicillin fermentation?

<p>To act as buffers to maintain pH (A)</p> Signup and view all the answers

What is the advantage of using fungal spores for steroid transformation?

<p>Simple medium and ease of product recovery (D)</p> Signup and view all the answers

What is the result of incubating Streptomyces spores with chloramphenicol?

<p>Chloramphenicol inactivated (A)</p> Signup and view all the answers

What is the optimal incubation time for spore-catalyzed chloramphenicol transformation activity?

<p>12 hours (C)</p> Signup and view all the answers

What is the effect of adding glucose or acetate to the spores during incubation?

<p>Maximum activity is maintained (B)</p> Signup and view all the answers

What is the role of penicillin G acylase in biotransformation?

<p>It transforms natural penicillin G to semi-synthetic penicillins (D)</p> Signup and view all the answers

What is the benefit of using microbial fermentation for antibiotic production?

<p>It reduces production costs (B)</p> Signup and view all the answers

What is the primary role of penicillin acylase in the industrial production of β-lactam antibiotics?

<p>To hydrolyze the side chain of penicillin G and related β-lactam antibiotics (D)</p> Signup and view all the answers

What is the difference between fermentation and biotransformation?

<p>Fermentation is a metabolic process, while biotransformation is a chemical change in a compound (D)</p> Signup and view all the answers

Which of the following microorganisms is used in the industrial production of penicillin acylase?

<p>Escherichia coli strain ATCC 11105 (D)</p> Signup and view all the answers

What is the product of penicillin acylase hydrolysis of penicillin G?

<p>6-APA (B)</p> Signup and view all the answers

What is the primary application of enzymatic biotransformation in the industrial production of β-lactam antibiotics?

<p>To convert penicillin G into semi-synthetic penicillins (D)</p> Signup and view all the answers

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