Microbial Metabolism and Metabolic Diversity and Functional Diversity (Lec 12)
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Questions and Answers

Which type of work involves the synthesis of complex molecules?

  • Metabolic work
  • Mechanical work
  • Chemical work (correct)
  • Transport work
  • What is the most commonly used form of cellular energy?

  • ATP (correct)
  • Poly-β-hydroxybutyrate
  • Phosphoenolpyruvate
  • Glycogen
  • Which compounds are produced by microorganisms for longer-term energy storage?

  • Glycogen (correct)
  • Elemental sulfur
  • ATP
  • Phosphoenolpyruvate
  • What is the tendency to donate or accept electrons expressed as?

    <p>Reduction potential</p> Signup and view all the answers

    Which type of electron donors have low reduction potential?

    <p>Good electron donors</p> Signup and view all the answers

    What is the best electron acceptor in terms of potential energy?

    <p>Highest potential energy</p> Signup and view all the answers

    What is continuously hydrolyzed and resynthesized in cells?

    <p>ATP</p> Signup and view all the answers

    What should you consider when analyzing microbial metabolism?

    <p>Both A and B</p> Signup and view all the answers

    What is the best terminal electron acceptor in microbial metabolism?

    <p>Oxygen</p> Signup and view all the answers

    Which type of bacteria is involved in nitrogen fixation and not photosynthesis?

    <p>Filamentous (with heterocysts)</p> Signup and view all the answers

    Where are cyanobacteria widely distributed?

    <p>Terrestrial, freshwater, and marine habitats</p> Signup and view all the answers

    What is the main function of heterocysts in cyanobacteria?

    <p>Nitrogen fixation</p> Signup and view all the answers

    Which type of bacteria uses hydrogen sulfide (H2S) as an electron donor for photosynthesis?

    <p>Purple Sulfur Bacteria</p> Signup and view all the answers

    What is the storage form of elemental sulfur (S) in purple sulfur bacteria?

    <p>Globules inside or outside of cells</p> Signup and view all the answers

    Which type of bacteria uses light for energy but uses an organic compound for carbon building blocks?

    <p>Photoheterotrophs</p> Signup and view all the answers

    In which type of bacteria do all four possible combinations of obtaining carbon and ATP exist?

    <p>Chemoautotrophs</p> Signup and view all the answers

    What do microorganisms play key roles in the cycling of?

    <p>Carbon, sulfur, nitrogen, and iron</p> Signup and view all the answers

    Which type of bacteria can be a phototrophic component of lichens?

    <p>Cyanobacteria</p> Signup and view all the answers

    Where are extensive crusts of cyanobacteria often found?

    <p>Desert soils</p> Signup and view all the answers

    Which type of microorganisms conserve energy from chemicals?

    <p>Chemotrophs</p> Signup and view all the answers

    What is the main difference between chemoorganotrophs and chemolithotrophs?

    <p>Chemoorganotrophs use organic compounds as electron donors, while chemolithotrophs use inorganic compounds</p> Signup and view all the answers

    Which process involves the reduction of an inorganic compound used as an electron acceptor for anaerobic respiration?

    <p>Dissimilative metabolism</p> Signup and view all the answers

    Which group of microorganisms can use hydrogen sulfide (H2S) as an electron donor?

    <p>Sulfur bacteria</p> Signup and view all the answers

    Which type of bacteria can use hydrogen gas (H2) as an electron donor?

    <p>Hydrogen-oxidizing bacteria</p> Signup and view all the answers

    What is the main difference between anoxygenic and oxygenic phototrophs?

    <p>Anoxygenic phototrophs use bacteriochlorophylls as photosynthetic pigments, while oxygenic phototrophs use chlorophyll</p> Signup and view all the answers

    Which group of microorganisms can use H2O as an electron donor for photosynthesis?

    <p>Cyanobacteria</p> Signup and view all the answers

    Which group of microorganisms can use H2S or H2 as electron donors for photosynthesis?

    <p>Purple and green bacteria</p> Signup and view all the answers

    Which type of bacteria can use lactate or pyruvate as electron donors for sulfur reduction?

    <p>Sulfur-reducing bacteria</p> Signup and view all the answers

    Which type of bacteria can grow autotrophically with H2 as the sole electron donor and O2 as the electron acceptor?

    <p>Hydrogen-oxidizing bacteria</p> Signup and view all the answers

    Reduction of nitrate (NO3-) to nitrate (NO2-) at the last step of the electron transport chain in Pseudomonas is an example of:

    <p>Anaerobic Respiratory</p> Signup and view all the answers

    Oxidation of H2 in aerobic H2-oxidizing bacteria is mediated by and the proton motive force generates .

    <p>Hydrogenases: ATP</p> Signup and view all the answers

    Which of the following is NOT a substrate that sulfur-oxidizing bacteria commonly use to support growth?

    <p>SO4 2-(Sulfate)</p> Signup and view all the answers

    Microorganism that obtain energy from the oxidation of lactose would be categorized as:

    <p>Chemoorganotrophs</p> Signup and view all the answers

    Which of the following is NOT a terminal electron accepter in anaerobic conditions?

    <p>Hydrogen sulfide</p> Signup and view all the answers

    Dependent on the presence or absence of oxygen, pyruvate can be metabolized along two major routes?

    <p>Respiration and Fermentation</p> Signup and view all the answers

    Aerobic H2 oxidation is mediated by. , and energy is ultimately produced using. and ATPase?

    <p>hydrogenase/ a proton motive force</p> Signup and view all the answers

    Study Notes

    Organic Chemistry

    • Synthesis of complex molecules involves work in organic chemistry
    • ATP is the most commonly used form of cellular energy

    Energy Storage

    • Microorganisms produce glycogen, starch, and polyhydroxyalkanoates for longer-term energy storage

    Electron Transfer

    • Electron-donating tendency is expressed as reduction potential
    • Electron donors with low reduction potential include hydrogen gas (H2) and hydrogen sulfide (H2S)
    • Oxygen (O2) is the best electron acceptor in terms of potential energy

    Cellular Metabolism

    • ATP is continuously hydrolyzed and resynthesized in cells
    • When analyzing microbial metabolism, consider the electron donors, acceptors, and ATP yield

    Microbial Metabolism

    • Oxygen (O2) is the best terminal electron acceptor in microbial metabolism
    • Nitrogen-fixing bacteria, such as cyanobacteria, are not photosynthetic
    • Cyanobacteria are widely distributed in aquatic and terrestrial environments

    Cyanobacteria

    • Heterocysts are specialized cells in cyanobacteria that function in nitrogen fixation
    • Green sulfur bacteria use hydrogen sulfide (H2S) as an electron donor for photosynthesis
    • Purple sulfur bacteria store elemental sulfur (S) in the form of sulfur globules

    Phototrophic Bacteria

    • Some bacteria use light for energy but use organic compounds for carbon building blocks
    • In some bacteria, all four possible combinations of obtaining carbon and ATP exist
    • Microorganisms play key roles in the cycling of nutrients, including carbon, nitrogen, and sulfur

    Chemotrophic Bacteria

    • Chemolithotrophs conserve energy from chemicals, whereas chemoorganotrophs conserve energy from organic compounds
    • The main difference between chemoorganotrophs and chemolithotrophs is the source of energy

    Anaerobic Respiration

    • Anaerobic respiration involves the reduction of an inorganic compound used as an electron acceptor

    Electron Donors

    • Hydrogen sulfide (H2S) and hydrogen gas (H2) can be used as electron donors
    • Sulfur-reducing bacteria can use lactate or pyruvate as electron donors for sulfur reduction

    Autotrophic Growth

    • Some bacteria can grow autotrophically with H2 as the sole electron donor and O2 as the electron acceptor

    Nitrate Reduction

    • Reduction of nitrate (NO3-) to nitrate (NO2-) is an example of anaerobic respiration in Pseudomonas

    H2 Oxidation

    • Oxidation of H2 in aerobic H2-oxidizing bacteria is mediated by hydrogenases and the proton motive force generates ATP
    • Aerobic H2 oxidation is mediated by hydrogenases, and energy is ultimately produced using oxidative phosphorylation and ATPase

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    Description

    Test your knowledge on microbial metabolism and metabolic diversity in this quiz. Explore the various types of work microbial cells must perform to survive and reproduce. Challenge yourself with questions on chemical synthesis, nutrient transport, and mechanical functions.

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