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Questions and Answers
What factor largely determines whether bacteria are pathogenic?
What factor largely determines whether bacteria are pathogenic?
Which of the following describes pleomorphic bacteria?
Which of the following describes pleomorphic bacteria?
Which method is used to classify bacteria based on their cell wall composition?
Which method is used to classify bacteria based on their cell wall composition?
What do bacteria that can metabolize lactose indicate about their metabolic activity?
What do bacteria that can metabolize lactose indicate about their metabolic activity?
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Which term refers to the classification of bacteria based on their observable characteristics?
Which term refers to the classification of bacteria based on their observable characteristics?
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What is the role of the mosquito in the life cycle of Plasmodium?
What is the role of the mosquito in the life cycle of Plasmodium?
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Which of the following is NOT a habitat where parasites can reside?
Which of the following is NOT a habitat where parasites can reside?
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From which of the following sources can parasitic infections NOT commonly originate?
From which of the following sources can parasitic infections NOT commonly originate?
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What are endoparasites known for?
What are endoparasites known for?
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How can cats contribute to the transmission of toxoplasmosis?
How can cats contribute to the transmission of toxoplasmosis?
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Why is it important for pregnant individuals to avoid contact with litter trays?
Why is it important for pregnant individuals to avoid contact with litter trays?
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Which of the following types of hosts is characterized as carrying a parasite but not necessarily showing symptoms of disease?
Which of the following types of hosts is characterized as carrying a parasite but not necessarily showing symptoms of disease?
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What is a key feature that differentiates permanent parasites from temporary parasites?
What is a key feature that differentiates permanent parasites from temporary parasites?
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In which type of host does the adult or sexually reproducing form of the parasite primarily live?
In which type of host does the adult or sexually reproducing form of the parasite primarily live?
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Opportunistic parasites are most likely to cause diseases in which type of hosts?
Opportunistic parasites are most likely to cause diseases in which type of hosts?
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What factor can result in some genes being absent in certain organisms due to their evolutionary history?
What factor can result in some genes being absent in certain organisms due to their evolutionary history?
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What mechanism allows unrelated bacteria to acquire resistance genes from others?
What mechanism allows unrelated bacteria to acquire resistance genes from others?
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How do Gram-positive bacteria generally develop resistance to Penicillin?
How do Gram-positive bacteria generally develop resistance to Penicillin?
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Which of the following is NOT a component of functional diversity in microorganisms?
Which of the following is NOT a component of functional diversity in microorganisms?
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What is a key reason why function and phylogenetic correlation may not always align?
What is a key reason why function and phylogenetic correlation may not always align?
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What type of testing can be used to assess the metabolic activities of microorganisms?
What type of testing can be used to assess the metabolic activities of microorganisms?
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How do Gram-negative bacteria primarily resist Penicillin?
How do Gram-negative bacteria primarily resist Penicillin?
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What type of environment can microorganisms inhabit?
What type of environment can microorganisms inhabit?
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How do fungi primarily grow and spread?
How do fungi primarily grow and spread?
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What is a method of asexual reproduction in yeast?
What is a method of asexual reproduction in yeast?
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What enables mycelium to develop and disperse spores?
What enables mycelium to develop and disperse spores?
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Which process introduces genetic variation in fungi?
Which process introduces genetic variation in fungi?
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Which factor is NOT mentioned as a potential driver for dimorphism in Ascomycetes?
Which factor is NOT mentioned as a potential driver for dimorphism in Ascomycetes?
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What happens during sporulation?
What happens during sporulation?
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In sexual reproduction of yeast, how many haploid ascospores are formed after meiosis?
In sexual reproduction of yeast, how many haploid ascospores are formed after meiosis?
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What best characterizes the spores produced by fungi?
What best characterizes the spores produced by fungi?
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What can result from the feeding habits of a parasite?
What can result from the feeding habits of a parasite?
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Which of the following statements about direct life cycles of parasites is true?
Which of the following statements about direct life cycles of parasites is true?
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What differentiates protozoa from metazoa in terms of classification?
What differentiates protozoa from metazoa in terms of classification?
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Which of the following is an example of a nematode?
Which of the following is an example of a nematode?
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What role do ticks play in relation to zoonotic diseases?
What role do ticks play in relation to zoonotic diseases?
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What is a common characteristic of arthropods?
What is a common characteristic of arthropods?
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Which organism is classified as a systemic protozoan parasite?
Which organism is classified as a systemic protozoan parasite?
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What is a defining feature of helminths?
What is a defining feature of helminths?
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Which disease is caused by the protozoan Trypanosoma brucei?
Which disease is caused by the protozoan Trypanosoma brucei?
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How do nematodes typically enter a new host?
How do nematodes typically enter a new host?
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What is swine flu scientifically known as?
What is swine flu scientifically known as?
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What type of parasite are fleas classified as?
What type of parasite are fleas classified as?
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What is a significant impact of viruses on human social behavior?
What is a significant impact of viruses on human social behavior?
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What is the primary concern regarding the governmental response to swine flu in Egypt?
What is the primary concern regarding the governmental response to swine flu in Egypt?
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Study Notes
- Microbial diversity, bacteria, session 1, 2/12/2024
Bacterial Nomenclature
- Bacteria can be beneficial or harmful; environment is critical
- Bacteria have relationships with their environment
- Bacteria can be classified as pathogenic or not
Bacterial Taxonomy/Nomenclature
- Bacterial names have different origins
- Scientists, places, colors
- Staphylococcus aureus = grape-like clusters under microscope
- Numerous ways to classify bacteria
Phenotypic Classifications
- Cell morphology – appearance under microscope, movement
- Pleiomorphic bacteria – change shape based on environment
- Metabolic activity – respiration, lactose metabolism or alternative sources
- Staining – distinguishing by cell wall or membrane, Gram staining
- Pathogenicity – antibiotic and heavy metal resistance, toxins, harm
Nutritional needs
- DNA based
- G-C content
16S rRNA sequencing
- Used to classify archaea and bacteria, useful to understand diversity
- 16s has regions that vary depending on species or organism
18s RNA sequencing
- Used to classify eukaryotic species (or organisms)
Metagenomics
- Sequencing of the whole sample, looking at overall genetic variation
- No need for culture, identifying organisms without isolating
Functional Diversity
- Physiological functions – metabolism, biochemistry, and processes
- Morphology – appearance on plates, under microscope, staining
- Ecological role, interaction with other microbial species, environment
Microbial Habitats
- Microbes are found everywhere
- Thermophiles – thrive in high temperatures
- Psychrophiles – thrive in low temperatures
- Halophiles – thrive in high-salt environments
Diversity of Bacteria
- 55 bacterial phyla
- Many more are in Candidatus status
Bacteria Phyla
- Proteobacteria
- Alpha, beta, gamma, delta, epsilon, and zeta proteobacteria
- Six classes
- 1 Alphaproteobacteria
- Many are anaerobic, prefer low-nutrition
- 2 Betaproteobacteria
- Can be nitrogen fixers
- 3 Gammaproteobacteria
Classification of Bacteria
- Shape
- Growth needs (oxygen demand)
- pH
- Mode of nutrition
- Osmotic pressure
- Temperature
- Number of flagella
- Spore formation
- Others
Bacterial Phyla – Specific Examples
- Thermophilic phyla (e.g., Aquificae/Aquificota)
- Have a flagella that enables motility
- Don't have endospores
- Most are chemolithoautotrophs – produce energy from chemical sources
- Deinococcota
- Very thick cell wall, two membranes
- Heterotrophic
- Strictly aerobic
- Can resist ionizing and UV radiation
Chloroflexi/ Chloroflexota
- Tend to be green due to proteins/chemicals inside
- Lack an outer membrane
- Generally, do not stain Gram positive but mostly negative
- Found in moderate thermophiles (around 80 degrees Celcius)
- Long and filamentous morphology
Cyanobacteria
- Photosynthetic species
- Often green from chlorophyll
- Chlorophyll is present in carboxysomes – CO2 fixation occurs there
- Responsible for free oxygen in the atmosphere
- Found in aquatic and land environments
Spirochaetota
- Spiral/helical shape
- Almost all are Gram-negative
- Majority are anaerobes or facultative anaerobes
- Flagella helps them swim, which are called endo flagella
- Treponema pallidum – syphilis, Borrelia burgdorferi – Lyme disease
Chlamydiota
- Intracellular parasites
- Require a host cell to reproduce
- Stain gram negative
- Unique life cycles, (elementary bodies, inclusion bodies)
Firmicutes
- Gram-positive, very low GC content
- Includes cocci bacteria
- Many are facultative anaerobes
- Some produce endospores for survival
- Examples: bacteria used for cheese production
Bacteriodetes
- Gram-negative
- Many can break down complex polysaccharides
- Are mostly obligate anaerobes
- Found in animal intestines, but are generally commensal
Diversity of Archaea
- Very recent research
- Many are identified and characterized by more research
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Description
Explore the fascinating world of bacteria in this quiz focused on bacterial nomenclature and taxonomy. Understand the various classifications of bacteria based on their morphology, metabolism, and pathogenicity. Dive into the significance of 16S rRNA sequencing in unraveling microbial diversity.