Microbial: Bacterial and Archaeal Traits

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Questions and Answers

Compare and contrast the cell wall composition of bacteria and fungi, and briefly explain how these differences are exploited in antimicrobial therapies.

Bacterial cell walls contain peptidoglycan, while fungal cell walls contain chitin. Antimicrobials like penicillin target peptidoglycan synthesis, selectively inhibiting bacteria. Antifungal medications target chitin.

Describe three different mechanisms of horizontal gene transfer in bacteria and briefly explain how these mechanisms contribute to the spread of antibiotic resistance.

Conjugation (transfer of plasmid DNA via direct contact), transduction (transfer of DNA via bacteriophages), and transformation (uptake of free DNA). These mechanisms spread resistance genes among bacteria.

Explain the difference between aerobic respiration, anaerobic respiration, and fermentation in the context of microbial metabolism. Give an example of a microorganism that utilizes each process.

Aerobic respiration uses oxygen as the final electron acceptor, anaerobic respiration uses other inorganic molecules, and fermentation uses organic molecules. Escherichia coli for aerobic, Pseudomonas for anaerobic, and Saccharomyces cerevisiae for fermentation are examples.

Briefly define 'bacteriophage' and explain its potential application in treating bacterial infections, including a possible advantage over traditional antibiotics.

<p>A bacteriophage is a virus that infects bacteria. They can be used in phage therapy to target and kill specific bacteria. An advantage is their specificity, which reduces the risk of off-target effects on the host microbiome.</p> Signup and view all the answers

Describe the main differences between bacteria and archaea, focusing on cell wall structure, membrane lipids, and typical environments inhabited.

<p>Bacteria have peptidoglycan in their cell walls, archaea do not. Archaeal membrane lipids differ chemically, and archaea often inhabit extreme environments.</p> Signup and view all the answers

Explain the concept of 'microbial ecology' and provide two examples of how microbial communities contribute to ecosystem functions.

<p>Microbial ecology studies the interactions of microbes with each other and their environment. Examples: nutrient cycling (e.g., nitrogen fixation) and decomposition of organic matter.</p> Signup and view all the answers

Discuss the roles of fungi as both beneficial organisms and as agents of disease, providing an example of each.

<p>Beneficial: decomposers, nutrient cyclers, food production (e.g., yeast in bread), medicine (e.g. penicillin). Disease: pathogens in plants and animals (e.g., athlete's foot).</p> Signup and view all the answers

Outline the key structural and functional differences between viruses and bacteria, explaining why viruses are considered non-living.

<p>Viruses are acellular, containing only genetic material (DNA or RNA) and a protein coat (capsid); bacteria are cellular, with organelles and metabolic capabilities. Viruses require a host to replicate, lacking independent reproductive machinery.</p> Signup and view all the answers

Describe the variety of nutritional strategies exhibited by protists, and provide an example of a protist for each strategy.

<p>Autotrophy (e.g., algae), heterotrophy (e.g., protozoa), and mixotrophy (both autotrophic and heterotrophic, e.g., euglena).</p> Signup and view all the answers

Explain the concept of 'virulence factors' in microbial pathogenesis, and provide two examples of how these factors contribute to disease.

<p>Virulence factors are traits that enhance a pathogen's ability to cause disease. Examples: toxins (damage host tissues) and adhesion factors (allow attachment to host cells).</p> Signup and view all the answers

Flashcards

Bacteria

Single-celled prokaryotic organisms lacking a nucleus and membrane-bound organelles, with peptidoglycan cell walls, reproducing via binary fission.

Archaea

Prokaryotic microorganisms often in extreme environments; cell walls lack peptidoglycan; genetically distinct from bacteria and eukaryotes.

Fungi

Eukaryotic organisms, can be unicellular (yeasts) or multicellular (molds); chitin cell walls; heterotrophic and reproduce via spores.

Protists

Diverse eukaryotic microorganisms, neither fungi, animals, nor plants; unicellular or multicellular; varied nutrition and reproduction.

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Virus

Acellular entities with DNA or RNA in a capsid, require a host to replicate.

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Microbial Ecology

The study of interactions between microorganisms and their environment.

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Microbial Genetics

Explores inheritance and variation in microorganisms; involves horizontal gene transfer and mutations.

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Microbial Metabolism

Chemical processes microbes use for energy and nutrients; includes aerobic/anaerobic respiration and fermentation.

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Microbial Pathogenesis

Study of how microorganisms cause disease through toxins, tissue invasion, and evading host defenses.

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Study Notes

  • Microbial pertains to microorganisms, which are microscopic organisms like bacteria, archaea, fungi, protists, and viruses.

Bacterial Characteristics

  • Bacteria are single-celled prokaryotic organisms lacking a nucleus and other membrane-bound organelles.
  • Their cell wall usually consists of peptidoglycan.
  • Reproduction typically occurs through binary fission, a form of asexual reproduction.
  • Bacteria come in diverse shapes, including cocci (spherical), bacilli (rod-shaped), and spirilla (spiral).
  • Many bacteria use flagella for movement.
  • Bacteria are vital in nutrient cycling, decomposition, and symbiotic relationships.
  • Some bacteria are pathogenic, causing diseases in humans, animals, and plants.

Archaeal Traits

  • Archaea are prokaryotic microorganisms that often live in extreme environments like hot springs and salt lakes.
  • Their cell walls don't have peptidoglycan, and their membrane lipids differ from bacteria and eukaryotes.
  • Genetically, archaea are distinct from bacteria and eukaryotes.
  • They participate in biogeochemical cycles, including methanogenesis.
  • Some archaea are extremophiles, thriving in high temperatures, salinity, or acidity.
  • No archaea are known to be pathogenic.

Fungal Attributes

  • Fungi are eukaryotic organisms with cells containing a nucleus and other organelles.
  • Fungi can be unicellular (yeasts) or multicellular (molds and mushrooms).
  • Fungal cell walls are made of chitin.
  • Fungi are heterotrophic, getting nutrients from organic matter.
  • They reproduce sexually and asexually through spores.
  • Fungi function as decomposers, pathogens, and in symbiotic relationships like mycorrhizae.
  • Certain fungi are used in food production (e.g., yeast in bread) and medicine (e.g., penicillin).

Protistan Features

  • Protists are a diverse group of eukaryotic microorganisms that are not fungi, animals, or plants.
  • They can be unicellular or multicellular.
  • Protists use a wide range of nutritional strategies, including autotrophy, heterotrophy, and mixotrophy.
  • They reproduce sexually and asexually.
  • Algae, protozoa, and slime molds are types of protists.
  • Certain protists are key primary producers in aquatic ecosystems.
  • Others are human pathogens, causing diseases like malaria and giardiasis.

Viral Properties

  • Viruses are acellular and not considered living organisms.
  • They consist of genetic material (DNA or RNA) inside a protein coat called a capsid.
  • Viruses need a host cell to replicate because they lack the machinery for independent reproduction.
  • They infect diverse organisms, including bacteria, archaea, fungi, protists, plants, and animals.
  • Viral infections can cause various diseases.
  • Bacteriophages are viruses infecting bacteria.

Microbial Ecology

  • Microbial ecology studies how microorganisms interact with each other and their environment.
  • Microorganisms live in diverse habitats, including soil, water, air, and plant and animal bodies.
  • They form complex communities and participate in ecological processes.
  • Microbes are essential for nutrient cycling, decomposition, and bioremediation.
  • Microbial interactions may be competitive, cooperative, or symbiotic.
  • The human microbiome includes microorganisms living in and on the human body.

Microbial Genetics

  • Microbial genetics studies the inheritance and variation of microorganisms.
  • Bacteria and archaea have a single circular chromosome.
  • Microorganisms exchange genetic material through horizontal gene transfer mechanisms like conjugation, transduction, and transformation.
  • Genetic mutations can result in antibiotic resistance and other adaptations.
  • Microbial genetics is applied in biotechnology, medicine, and agriculture.

Microbial Metabolism

  • Microbial metabolism includes the chemical processes that microorganisms use for energy and nutrients.
  • Microbes have diverse metabolic pathways, including aerobic respiration, anaerobic respiration, and fermentation.
  • Some microbes are autotrophs, using inorganic compounds or light as energy sources.
  • Others are heterotrophs, getting energy from organic compounds.
  • Microbial metabolism is critical in biogeochemical cycles like the carbon and nitrogen cycles.

Microbial Pathogenesis

  • Microbial pathogenesis studies how microorganisms cause disease.
  • Pathogens may produce toxins, invade tissues, and evade host defenses.
  • Virulence factors enhance a pathogen's ability to cause disease.
  • The immune system defends against microbial infections through innate and adaptive immunity.
  • Antibiotics and other antimicrobial drugs treat bacterial infections.
  • Vaccines can protect against viral and bacterial diseases.

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