Microbes and Their Impact on Human History
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Questions and Answers

What is the main characteristic that distinguishes prokaryotes from eukaryotes?

  • Size and complexity
  • Presence of a nucleus (correct)
  • Type of genetic material
  • Mode of reproduction

Who is credited with the discovery of the largest bacterium?

  • Louis Pasteur
  • Alexander Fleming
  • Sergei Winogradsky (correct)
  • Robert Koch

Which type of microbe requires a microscope to be seen?

  • Bacteriophage
  • Prion
  • Protozoa (correct)
  • Yeast

What is the significance of metagenomes in understanding microbial populations?

<p>They capture the genetic diversity of entire microbial communities (B)</p> Signup and view all the answers

What was Lynn Margulis's proposed mechanism for the evolution of eukaryotic organelles?

<p>Endosymbiosis (B)</p> Signup and view all the answers

What did Carl Woese's analysis of DNA from prokaryotes living in hot springs reveal?

<p>They were a distinct form of life (C)</p> Signup and view all the answers

What did microbiologists do to define microbial species despite the difficulty in defining them?

<p>Devised working definitions of microbial species (C)</p> Signup and view all the answers

How did Woese's discovery impact the classification scheme of five kingdoms?

<p>It replaced the five kingdoms with three equally distinct groups called domains (B)</p> Signup and view all the answers

What substance did Alexander Fleming discover that kills bacteria?

<p>Penicillium mold (A)</p> Signup and view all the answers

What is the main purpose of immunization?

<p>To stimulate an immune response by inoculation with an attenuated pathogen (B)</p> Signup and view all the answers

What did Sergei Winogradsky and others demonstrate about the importance of bacteria?

<p>Bacteria play a crucial role in geochemical cycling (C)</p> Signup and view all the answers

What are endosymbionts?

<p>Microbes that live inside a larger host organism (A)</p> Signup and view all the answers

What is resolution in the context of observing microbes?

<p>The smallest distance by which two objects can be separated and still be distinguished (B)</p> Signup and view all the answers

What does detection refer to in the context of observing microbes?

<p>The ability to determine the presence of an object (C)</p> Signup and view all the answers

What does magnification mean in the context of observing microbes?

<p>An increase in the apparent size of an image to resolve smaller separations between objects (B)</p> Signup and view all the answers

Which type of microscopy is described as the simplest?

<p>Light microscopy (C)</p> Signup and view all the answers

What is the main difference between a simple stain and a differential stain?

<p>A simple stain adds dark color specifically to cells, but not to the external medium or surrounding tissue, while a differential stain stains types of cell differently to distinguish them (D)</p> Signup and view all the answers

Which type of bacteria retains the crystal violet stain in the Gram stain?

<p>Gram-positive bacteria (D)</p> Signup and view all the answers

What is the main use of fluorescence microscopy in microbiology?

<p>To label specific cells even in environmental samples (C)</p> Signup and view all the answers

What do molecules with fluorescence properties (fluorophores) do in fluorescence microscopy?

<p>They absorb light of lower energy and then emit light of higher energy (D)</p> Signup and view all the answers

What is the size range of prokaryotes?

<p>0.4–10 µm (C)</p> Signup and view all the answers

Which shapes of bacteria are common to many taxonomic groups?

<p>Bacilli: rods, Cocci: spheres (D)</p> Signup and view all the answers

What must exist for electromagnetic radiation to resolve an object?

<p>Contrast between object and its medium, Wavelength smaller than the object, Enough light intensity (A)</p> Signup and view all the answers

What is the wavelength range of visible light?

<p>~400–750 nm (A)</p> Signup and view all the answers

What type of interaction occurs when the wavefront bounces off the surface of an object?

<p>Reflection (B)</p> Signup and view all the answers

In bright-field microscopy, what is used to increase resolution?

<p>Shorter-wavelength light (D)</p> Signup and view all the answers

What is the total magnification when the ocular lens has a magnification of 5X and the objective lens has a magnification of 20X?

<p>100X (B)</p> Signup and view all the answers

How does fixation enhance the detection and resolution of cells under a microscope?

<p>By making cells adhere to a slide in a fixed position (A)</p> Signup and view all the answers

What is the size range of prokaryotes?

<p>0.4–10 µm (A)</p> Signup and view all the answers

What are the common shapes of bacteria found in many taxonomic groups?

<p>Rods and spheres (D)</p> Signup and view all the answers

What is the wavelength range of visible light?

<p>~400–750 nm (B)</p> Signup and view all the answers

What conditions must exist for electromagnetic radiation to resolve an object?

<p>Contrast between object and its medium, wavelength smaller than the object, and others (C)</p> Signup and view all the answers

What is part of the spectrum of electromagnetic radiation?

<p>Light (A)</p> Signup and view all the answers

What is a subcellular structure that is too small to resolve by light microscopy?

<p>Subcellular structures are too small to resolve by light microscopy (A)</p> Signup and view all the answers

What is the main characteristic that distinguishes prokaryotes from eukaryotes?

<p>Lack of a true nucleus and other membrane-bound organelles (A)</p> Signup and view all the answers

What is the main purpose of a simple stain?

<p>To add dark color specifically to cells (B)</p> Signup and view all the answers

What distinguishes a differential stain from a simple stain?

<p>A differential stain stains types of cell differently to distinguish them (C)</p> Signup and view all the answers

Which type of bacteria retains the crystal violet stain in the Gram stain?

<p>Gram-positive bacteria (B)</p> Signup and view all the answers

What does fluorescence microscopy allow scientists to do?

<p>Label specific cells even in environmental samples (D)</p> Signup and view all the answers

What happens when a specimen absorbs light of a defined wavelength and then emits light of lower energy?

<p>It fluoresces (B)</p> Signup and view all the answers

What is the most famous differential stain mentioned in the text?

<p>Gram stain (D)</p> Signup and view all the answers

What is the main characteristic of a differential stain?

<p>It stains types of cell differently to distinguish them (D)</p> Signup and view all the answers

Which type of bacteria do not retain the crystal violet stain in the Gram stain?

<p>Gram-negative bacteria (A)</p> Signup and view all the answers

What can fluorescence microscopy be used for in live organisms?

<p>To observe fluorescence. (A)</p> Signup and view all the answers

What is the definition of resolution in the context of observing microbes?

<p>The smallest distance by which two objects can be separated and still be distinguished (C)</p> Signup and view all the answers

What is the main difference between light microscopy and other types of microscopy?

<p>Light microscopy is the simplest, while other types are more complex (B)</p> Signup and view all the answers

What does detection refer to in the context of observing microbes?

<p>The ability to determine the presence of an object (A)</p> Signup and view all the answers

Which organisms fall within the size range that structures can be seen under a light microscope?

<p>Protozoa, algae, fungi (C)</p> Signup and view all the answers

What is the definition of magnification in the context of observing microbes?

<p>The increase in the apparent size of an image to resolve smaller separations between objects (C)</p> Signup and view all the answers

What is the significance of powerful microscopes in observing microbes?

<p>They enable detection of microbes in familiar and unexpected habitats (C)</p> Signup and view all the answers

What is meant by the term 'microbes differ in size, over a range of a few orders of magnitude'?

<p>Microbes have a wide range of sizes spanning several orders of magnitude (A)</p> Signup and view all the answers

What is the definition of absorption in the context of light interacting with an object?

<p>The acquisition of the photon’s energy by the absorbing object (D)</p> Signup and view all the answers

In bright-field microscopy, what technique is used to increase resolution?

<p>Use shorter-wavelength light (D)</p> Signup and view all the answers

What is the main purpose of fixation in microscopy?

<p>To enhance detection and resolution of cells (C)</p> Signup and view all the answers

What does refraction refer to in the context of magnification by a lens?

<p>The bending of light as it enters a substance that slows its speed (D)</p> Signup and view all the answers

How does total magnification in a compound microscope relate to the ocular and objective lenses?

<p>Total magnification is the product of the magnification of the ocular and objective lenses (A)</p> Signup and view all the answers

What occurs when the wavefront interacts with an object smaller than the wavelength of light?

<p>Scattering (D)</p> Signup and view all the answers

What is the main function of glass with curvature (lens) in magnification?

<p>To bend light rays to intersect at a focal point (A)</p> Signup and view all the answers

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