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Questions and Answers

What is the main component of the cell wall in Gram-negative bacteria?

  • Chitin
  • Lipopolysaccharides (correct)
  • Peptidoglycan
  • Cellulose
  • Which of the following correctly describes functions of the cytoplasmic membrane in bacteria?

  • It participates in DNA replication.
  • It stores nutrients and waste products.
  • It provides structural support and rigidity.
  • It is selectively permeable and involved in energy production. (correct)
  • Which of these structures in bacteria serves as an external attachment mechanism?

  • Ribosome
  • Pilus (correct)
  • Cytoplasm
  • Plasma membrane
  • What is the purpose of the bacterial glycocalyx?

    <p>To serve as a protective barrier and aid in adhesion. (A)</p> Signup and view all the answers

    In a typical bacterium, which component is NOT part of the cytoplasmic region?

    <p>Nucleus (C)</p> Signup and view all the answers

    Which type of cellular arrangement is characterized by pairs of cocci?

    <p>Diplococci (C)</p> Signup and view all the answers

    What defines a spirochete amongst spiral bacteria?

    <p>Flexible and thin structure. (C)</p> Signup and view all the answers

    Which arrangement results from bacteria dividing along multiple random planes?

    <p>Clusters of cells (B)</p> Signup and view all the answers

    What is the primary function of the outer membrane in Gram-negative bacteria?

    <p>To serve as a protective barrier (B)</p> Signup and view all the answers

    Which of the following is a characteristic feature of Gram-positive bacteria?

    <p>Lack of an outer membrane (C)</p> Signup and view all the answers

    What role does the bacterial glycocalyx play in bacterial virulence?

    <p>Prevents phagocytosis by white blood cells (B)</p> Signup and view all the answers

    What is primarily found in the periplasmic space of Gram-negative bacteria?

    <p>Cell wall components and enzymes (C)</p> Signup and view all the answers

    Which structure is responsible for the segregation of DNA during binary fission in bacteria?

    <p>Mesosome (C)</p> Signup and view all the answers

    Which of the following best describes the composition of capsules in bacteria?

    <p>An extensive, tightly bound accumulation of gelatinous material (B)</p> Signup and view all the answers

    What type of DNA do plasmids represent in bacterial cells?

    <p>Small circular extra-chromosomal DNA (A)</p> Signup and view all the answers

    How do capsules assist bacteria in establishing infections?

    <p>By allowing adherence to surfaces (B)</p> Signup and view all the answers

    What is the primary function of the peptidoglycan layer in bacterial cell walls?

    <p>Provides structural rigidity (B)</p> Signup and view all the answers

    Which of the following is a characteristic of Gram-negative bacteria?

    <p>Double membrane structure (B)</p> Signup and view all the answers

    Which component primarily distinguishes Gram-positive from Gram-negative bacteria?

    <p>Thickness of the peptidoglycan layer (D)</p> Signup and view all the answers

    What role do common pili (fimbriae) play in bacterial attachment?

    <p>Enable specific adherence to surfaces (A)</p> Signup and view all the answers

    What is the main structural component of the bacterial cell wall?

    <p>Peptidoglycan (C)</p> Signup and view all the answers

    During conjugation, what is the function of the sex pilus?

    <p>Transfers DNA between bacteria (D)</p> Signup and view all the answers

    Which part of the Gram-negative cell wall is responsible for the protein transport system?

    <p>Periplasmic space (D)</p> Signup and view all the answers

    What is the primary role of teichoic acids in Gram-positive bacteria?

    <p>Assist in maintaining cell shape (D)</p> Signup and view all the answers

    What does the glycocalyx primarily assist bacteria with?

    <p>Adhesion and protection (D)</p> Signup and view all the answers

    Which chemical structure provides rigidity to the bacterial cell wall?

    <p>Peptidoglycan (C)</p> Signup and view all the answers

    Flashcards

    Bacterial Capsule

    A tightly bound accumulation of gelatinous material surrounding the cell wall.

    Capsule Function

    Enhances bacterial virulence by preventing phagocytosis and allows adherence to surfaces.

    Bacterial Outer Membrane

    A layer external to the cell wall of Gram-negative bacteria, made of phospholipids, proteins, lipoproteins, and lipopolysaccharide

    Outer Membrane Function

    Acts as a protective barrier, hindering entry of harmful substances (like bile, antibiotics).

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    Periplasmic Space

    The space between the plasma membrane and cell wall, only in Gram-negative bacteria

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    Nuclear Area (Nucleoid)

    Region containing the single, circular chromosome (ccDNA) of bacteria.

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    Plasmids

    Small, circular DNA fragments, separate from the chromosome, with genes for extra functions (e.g., antibiotic resistance

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    Exoenzymes

    Enzymes secreted by bacteria to break down large molecules outside the cell.

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    Prokaryotic Cell

    A cell lacking a membrane-bound nucleus and organelles; its cell wall is usually peptidoglycan. It reproduces via binary fission.

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    Eukaryotic Cell

    A cell with a membrane-bound nucleus and organelles. Its cell wall (if present) is composed of cellulose or chitin; mitosis is its reproduction method

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    Bacterial Shape: Coccus

    A spherical-shaped bacterium.

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    Bacterial Shape: Bacillus

    A rod-shaped bacterium.

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    Bacterial Shape: Spiral

    A spiral-shaped bacterium.

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    Bacterial Arrangement: Diplococci

    Pairs of cocci (spherical bacteria) after cell division.

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    Bacterial Arrangement: Streptococci

    Chains of cocci after multiple divisions in a single plane

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    Bacterial Arrangement: Staphylococcus

    Clusters of cocci after divisions in multiple planes

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    Bacterial Pili

    Short, hair-like structures on bacterial surfaces. Used for attachment and conjugation (DNA transfer).

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    Fimbraie (Pili)

    Short, hair-like structures that help bacteria attach to surfaces.

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    Conjugation Pilus

    Long pili involved in transferring DNA between bacteria.

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    Cell Envelope

    The three layers (plasma membrane, cell wall, and capsule) surrounding a bacterial cell's protoplasm.

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    Bacterial Cell Wall

    Rigid structure surrounding the plasma membrane, providing shape and protection from bursting.

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    Gram-positive Cell Wall

    Thick peptidoglycan layer, composed of many layers.

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    Gram-negative Cell Wall

    Thin peptidoglycan layer, with an outer membrane.

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    Peptidoglycan

    Main component of bacterial cell walls, a polymer of sugars linked by amino acids.

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    Teichoic Acid

    Sugar polymers in Gram-positive walls that help maintain shape and cell division.

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    Periplasm

    Region in gram-negative bacteria between the outer membrane and the cytoplasmic membrane, holding proteins involved in transport systems.

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    Study Notes

    Prokaryotic vs Eukaryotic Cells

    • Prokaryotic cells lack a nucleus and other membrane-bound organelles.
    • Eukaryotic cells have a nucleus and other membrane-bound organelles.

    Prokaryotic Cell Structure

    • Appendages: Flagella (for movement), pili (for attachment and conjugation).
    • Cell Envelope: Capsule (protection and attachment), cell wall (strength and shape), plasma membrane (controls what enters and leaves the cell).
    • Cytoplasmic Region: Chromosome (genetic material), ribosomes (protein synthesis), plasmids (small, extra-chromosomal DNA circles), inclusions (storage granules), and gas vesicles.

    Eukaryotic Cell Structure

    • Nucleus: Contains the cell's genetic material (DNA) organized into chromosomes.
    • Organelles: Specialized structures with specific functions such as mitochondria, endoplasmic reticulum, Golgi apparatus, vacuoles, and lysosomes.
    • Cell membrane: Acts as a barrier to the entry and exit of substances into and out of the cell.
    • Cell wall: Present in some eukaryotic cells like plant and algal cells making them rigid and provides the cell with strength and structure, helping to retain shape.
    • Cytoplasm: Contains all cell components found between the cell membrane and the nucleus where organelles, cytoskeleton, cytosol and inclusions (storage granules) are located.

    Bacterial Morphology

    • Cocci (cocci): Spherical-shaped bacteria.
    • Bacilli (bacilli): Rod-shaped bacteria.
    • Spirilla: Spiral-shaped bacteria.
    • Vibrio: Curved, comma-shaped bacteria.
    • Coccobacilli :Short round bacteria
    • Pleomorphic bacteria: bacteria that can change shape.

    Bacterial Arrangements

    • Diplococci: Pairs of cocci.
    • Streptococci: Chains of cocci.
    • Staphylococci: Clusters of cocci.
    • Tetrads: Groups of four cocci.
    • Sarcina: Cubical packets of eight cocci.
    • Diplobacilli: Pairs of bacilli
    • Streptobacilli: Chains of bacilli
    • Coccobacilli: Short bacilli

    Bacterial Cell Walls

    • Gram-positive: Thick peptidoglycan layer, teichoic acids.
    • Gram-negative: Thin peptidoglycan layer, outer membrane, lipopolysaccharides, periplasmic space.

    Bacterial Cell Envelope

    • The outer covering of a bacterial cell consisting of capsule, cell wall and plasma membrane.
    • The cell wall is an essential structure. Peptidoglycan protects from osmotic rupture or lysis.

    Bacterial Motility

    • Flagella: Filamentous protein structures that enable movement by rotating like propellers.
    • Types of flagella include: monotrichous, lophotrichous, amphitrichous, peritrichous.
    • Pili and Fimbriae: Hair-like structures that help cells adhere to surfaces and in bacterial conjugation.
    • Types of pili include: common pili, sex pilus or F-pilus.

    Bacterial Internal Structures

    • Chromosome: Circular DNA molecule containing genetic information.
    • Plasmids: Smaller, circular DNA molecules separate from the chromosome and often carry genes for antibiotic resistance.
    • Ribosome: Site of protein synthesis, composed of large 50S subunit and small 30S subunit which combine to form 70S ribosome.
    • Inclusions/Granules: storage for nutrients.
    • Gas vesicles: allow bacteria to float.
    • Endospores: dormant structures resistant to harsh conditions.

    Techniques to Demonstrate Bacterial Motility

    • Flagellar stains.
    • Motility medium test.
    • Direct microscopic observation of living bacteria in wet mounts.

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