Metabolism Regulation and Study Methods Quiz

Metabolism refers to the chemical processes that occur within a living organism in order to maintain life. It involves the breakdown of molecules into simpler forms and the synthesis of complex molecules from simpler forms. Metabolism is regulated by various mechanisms to maintain a steady-state and respond to changes in the environment.

Methods to study metabolism include purification of proteins, subcellular fractionation, in vivo use of radioisotopes, purification based on solubility, charge, size, and binding abilities, amino acid sequence determination using Edman degradation, location determination using antibodies, and 3D structure determination using X-ray crystallography.

Radioisotopes can be used to label metabolic intermediates or products to track their movement within the organism. This can help identify the enzymes and pathways involved in metabolism.