Medical Parasitology: Protozoa and Amoebae

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Questions and Answers

What characteristic is unique to organisms classified under Ciliophora?

  • Formation of cysts as an infective stage.
  • Presence of pseudopodia for motility.
  • Coating of cilia on their cell surfaces. (correct)
  • Reproduction through binary fission.

Which of the following describes the key difference between a trophozoite and a cyst stage in protozoa?

  • Trophozoites are resistant to environmental changes, while cysts are susceptible.
  • Trophozoites are infective, while cysts are reproductive.
  • Trophozoites are motile and feeding, while cysts are quiescent and resistant. (correct)
  • Trophozoites reproduce sexually, while cysts reproduce asexually.

Entamoeba histolytica utilizes which mechanism for motility?

  • Ciliary action
  • Flagellar movement
  • Undulating membrane
  • Extension of pseudopodia (correct)

Which characteristic is commonly associated with Entamoeba coli?

<p>A cyst stage containing up to 8 nuclei. (D)</p> Signup and view all the answers

Which of the following protozoan groups is characterized by having at least one whiplike flagellum?

<p>Flagellates (B)</p> Signup and view all the answers

Which feature distinguishes Endolimax nana from other intestinal amoebae?

<p>A large, irregularly shaped karyosome in its trophozoite stage. (C)</p> Signup and view all the answers

What structural features are found in the trophozoites of Balantidium coli?

<p>Cilia covering the body and two types of nuclei (C)</p> Signup and view all the answers

Which of the following parasites reproduces through longitudinal binary fission?

<p>Giardia lamblia (D)</p> Signup and view all the answers

If a lab technician observes a stool sample containing cysts with 8 nuclei, what is the MOST likely parasite?

<p>Entamoeba coli (C)</p> Signup and view all the answers

Which parasite is typically transmitted sexually?

<p>Trichomonas vaginalis (A)</p> Signup and view all the answers

Which of the following is a key characteristic of Sporozoa?

<p>They undergo a complex life cycle with both sexual and asexual reproductive phases. (A)</p> Signup and view all the answers

Which of the following protozoa is known to possess both a macronucleus and a micronucleus?

<p>Balantidium coli (D)</p> Signup and view all the answers

What is the primary mode of transmission for Giardia lamblia?

<p>Ingestion of contaminated food or water (C)</p> Signup and view all the answers

What motility structure is absent in the trophozoite stage of Entamoeba histolytica?

<p>Flagella (A)</p> Signup and view all the answers

A stool sample is examined and found to contain trophozoites with a single nucleus and a characteristically large, irregularly shaped karyosome. Which organism is MOST likely present?

<p>Endolimax nana (A)</p> Signup and view all the answers

What is the typical shape and average size of Trichomonas vaginalis?

<p>Pear-shaped, 15-30 μm (D)</p> Signup and view all the answers

How does Entamoeba histolytica infection MOST commonly occur?

<p>By ingesting mature cysts in contaminated food or water (A)</p> Signup and view all the answers

What is the function of the cyst stage in protozoan parasites?

<p>To provide protection and survival outside the host (A)</p> Signup and view all the answers

Which protozoan parasite is commonly associated with causing infections in human females?

<p>Trichomonas vaginalis (B)</p> Signup and view all the answers

In which group of protozoa would you classify Plasmodium and Toxoplasma?

<p>Sporozoa (C)</p> Signup and view all the answers

Which of the following best describes the structure of Giardia lamblia?

<p>A pear-shaped cell with flagella and two nuclei (B)</p> Signup and view all the answers

What is a defining characteristic of Iodamoeba butschlii?

<p>Presence of a large glycogen vacuole in the cyst stage (B)</p> Signup and view all the answers

What is the mode of transmission for Balantidium coli to humans??

<p>Through the ingestion of cysts. (C)</p> Signup and view all the answers

An investigator suspects a waterborne outbreak of parasitic infection in a remote village. Testing reveals the presence of binucleate trophozoites in stool samples. Which parasite is the MOST likely culprit?

<p>Giardia lamblia (A)</p> Signup and view all the answers

What is one major factor differentiating pathogenic from non-pathogenic amoebae?

<p>The ability to ingest red blood cells (D)</p> Signup and view all the answers

Flashcards

What are Protozoa?

A diverse group of unicellular eukaryotic organisms.

What is a Trophozoite?

The active, motile feeding stage of a protozoan parasite.

What is a Cyst?

A quiescent, resistant, infective stage of a protozoan parasite.

How do amoebae move?

Motility is accomplished via extension of pseudopodia

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What is Entamoeba histolytica?

A parasitic amoeba that can cause amebic dysentery.

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What is Entamoeba coli?

Non-pathogenic intestinal ameba.

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What is Endolimax nana?

A non-pathogenic amoeba whose trophozoites measure 6–12 µm.

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What is Entamoeba hartmanni?

Trophozoites contain a single nucleus that contains a small, centrally located karyosome

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What is Iodamoeba butschlii?

Trophozoyt (8-20 µm) with a single nucleus with a large, usually centrally located karyosome

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What are Ciliophora?

Protozoa that move using numerous cilia.

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What is Ciliophora?

Protozoa possessing two types of nuclei within single cells.

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What is Balantidium coli?

The only ciliate known to be pathogenic to humans.

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What are Flagellates?

Protozoa that move using one or more flagella.

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What is Giardia lamblia?

A flagellate with two equal-sized nuclei and eight flagella.

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What is Trichomonas vaginalis?

A pear-shaped flagellate that causes infections in human females.

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What is an Axostyle?

A structure found in trophozoites that serves as an attachment point and skeletal support.

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What is a Trophozoite?

Actively motile feeding stage of a protozoan.

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What is a Cyst?

An infective dormant stage of a protozoan parasite.

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What is Pseudopodia?

Extension of the protoplasm

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What is Amebiasis?

Infection caused by Entamoeba histolytica

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What is Ciliophora?

Have a coating of cilia on the surfaces of their cell.

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What is micronucleus and macronucleus?

Two types of nuclei.

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What is a Cyst?

The infective stage and is acquired by the host through ingestion of contaminated food or water.

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What is Trichomonas vaginalis?

A flagellate that causes infections in human females.

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What is Gardia Lambia?

Two equal sized nuclei.

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Study Notes

  • Medical parasitology classifies parasites into protozoa and helminths.
  • Protozoa are unicellular organisms.
  • Helminths are multicellular organisms.

Protozoa Characteristics

  • Protozoa are unicellular organisms belonging to the Kingdom Protista.
  • They can transform from an active, motile trophozoite stage to an inactive, non-motile cyst stage.
  • Four main groups of protozoa are: Sarcomastigophora (amoebae), Zoomastigophora (flagellates), Apicomplexa (malaria and coccidia), and Ciliophora (ciliates).

Sarcomastigophora (Amoebae)

  • Trophozoite is the actively motile feeding stage.
  • Cyst is the quiescent, resistant, infective stage.
  • Reproduction occurs via binary fission.
  • Motility is achieved through the extension of pseudopodia.
  • Entamoeba histolytica is an example of an amoeba.

Entamoeba Histolytica

  • Mature cysts ingested.
  • Cysts and trophozoites passed in feces.
  • Multiplication occurs.
  • Excystation occurs.

Laboratory Diagnosis of Entamoeba Histolytica

  • Trophozoites have a single nucleus.
  • Cysts contain 1-4 nuclei.

Nonpathogenic Amoebae

  • Several types of nonpathogenic amoebae exist in the intestines, including: Entamoeba coli, Entamoeba hartmanni, Entamoeba polecki, Endolimax nana, and Iodamoeba buetschlii.

Entamoeba Coli

  • Trophozoites have a single nucleus and a large karyosome.
  • Cysts have 8 nuclei

Endolimax Nana

  • Trophozoites measure 6–12 µm and have a single nucleus with a characteristically large, irregularly shaped karyosome.
  • Cysts vary from spherical to ellipsoidal.
  • Cysts measure 5–10 µm and have four small nuclei with large, usually centrally located karyosomes.

Entamoeba Hartmanii

  • Trophozoites have a single nucleus that contains a small, centrally located karyosome.
  • Cysts are 7-9µ in diameter and contain 1 - 4 nuclei.

Iodamoeba Butschlii

  • Trophozoites measure 8–20 µm and have a single nucleus with a large, usually centrally located karyosome.
  • Cysts are nearly spherical to ellipsoidal in shape, measure 5–20 μm, and contain a single nucleus.

Ciliophora

  • Ciliophora are characterized by a coating of cilia on their cell surfaces.
  • Ciliophora has two types of nuclei within single cells: a micronucleus and a macronucleus.

Balantidium Coli

  • Trophozoites are covered with cilia and have a macronucleus and micronucleus.
  • Cysts are spherical with a single nucleus.

Balantidium Coli Infection

  • Cysts shed in formed stools
  • Both cysts and trophozoites may be shed in diarrheal stools.
  • binary fission occurs
  • The cyst is the infectious stage and is acquired by the host through ingestion of contaminated food or water.

Flagellates

  • Flagellates, such as Giardia and Trichomonas, are examples of flagellates.

Giardia Lamblia

  • Giardia Lamblia have two equal-sized nuclei
  • Giardia Lamblia have 8 flagella
  • Asexual reproduction is by longitudinal binary fission

Giardia Lamblia (Intestinalis)

  • Cysts are the dormant stage and can survive for weeks to months in cold water.
  • They absorb nutrients from the lumen of the small intestine and are anaerobes.

Trichomonas Vaginalis

  • Trichomonas Vaginalis is a 15 to 30 μm pear-shaped protozoon.
  • It has a small, very pale, eccentrically placed nucleus.
  • Causes infections in human females and is sexually transmitted.

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