Medical Biochemistry I Lab: Techniques Overview

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Questions and Answers

What should a student do if they develop symptoms such as fever or cough before attending lab?

Report the symptoms immediately. (correct)

Contact their friends for advice.

Attend the lab if they feel capable.

Wait to see if the symptoms improve.

Which of the following is NOT part of the mandatory safety gear required in the lab?

Lab coat with cuffed sleeves

Sunglasses (correct)

Safety shoes

Mask

What is the formula used to calculate the concentration of reagents during experiments?

C1/V1 = C2/V2 (correct)

C1/V2 = C2/V1

C1 + C2 = C3

C1 * V1 = C2 + V2

What must a student do before attending a lab if they have a justified absence?

Announce it before the beginning of the class. Signup and view all the answers

Which behavior is encouraged while working in the lab?

Working in silence. Signup and view all the answers

How should hair be styled for safety in the lab?

Tied back in a pony-tail. Signup and view all the answers

What is an essential practice regarding hand hygiene in the lab?

Wash hands with soap before wearing gloves. Signup and view all the answers

What should students do with regards to their laboratory notebook?

Take notes on everything explained. Signup and view all the answers

What is the primary purpose of using a Pipetman in a laboratory?

To accurately dispense liquid volumes Signup and view all the answers

What is the main purpose of disinfecting work surfaces before and after an experiment?

To prevent cross-contamination. Signup and view all the answers

What is the primary focus of Part C in the lab exercise?

Learning about dilutions. Signup and view all the answers

Which Pipetman would be most appropriate for dispensing 50 μl of a solution?

P100 Signup and view all the answers

What would be the weight of 100 μl of distilled water at room temperature?

0.1 g Signup and view all the answers

In a solution, the substance dissolved in the solvent is called what?

Solute Signup and view all the answers

What is the meaning of an aqueous solution?

A solution where water is the solvent Signup and view all the answers

How is the accuracy of a pipette commonly checked?

By weighing dispensed water Signup and view all the answers

When preparing a dilution, which component is typically concentrated?

The solute Signup and view all the answers

Which Pipetman would be used for volumes from 0.5 to 10 μl?

P10 Signup and view all the answers

If you dissolve 5 g of NaOH in 100 g of solution, what is the % by mass concentration?

5% Signup and view all the answers

What must a student do to communicate with a faculty member via email?

Use an EUC email account Signup and view all the answers

Study Notes

Course Information

Course: Medical Biochemistry I
Lab Exercise 1: Introduction to Techniques - Use of Pipetman, Sample Preparation and Dilutions
Semester: Fall 2024
Instructors: Andreou M & Kyriakou TC
Institution: European University Cyprus, School of Medicine

Objectives

Introduction: Lab characteristics/guidelines
Part A: Use of Pipetman (Theoretical Background and Exercises)
Part B: Sample Preparation (Main units of concentration and exercises)
Part C: Dilutions (Dilution Formula and exercises)

COVID-19 Safety Protocol

Be on time to avoid crowds.
Always bring protective gear (lab coat with cuffed sleeves, mask, goggles).
Students without all gear will not be admitted.
Practice hand hygiene (wash hands with soap, then gloves).
Disinfect working surfaces before and after experiments.
Report any symptoms immediately (fever, cough, breathing difficulties).

Absences

Mandatory participation required.
Physical presence or synchronous participation (permission required).
Two justified absences excused (severe health condition or emergency).
Absences must be announced before class begins.
Lab experiments cannot be repeated.

Preparation

Study uploaded material.
Print protocol/experimental methodology.
Identify materials/equipment needed from the protocol.
Be prepared for technically-demanding applications.
Closed-toe shoes, lab coat, goggles, and gloves required.
Hair must be tied back (ponytail).
No food/drinks allowed.

Lab Notebook

Record everything explained.
Note protocol applications with dates.
Calculate reagent concentrations (e.g., C1V1=C2V2).
Evaluate results and identify areas needing adjustment.

Lab Etiquette

Move carefully (as if in a bubble).
Be aware of your surroundings.
Work cooperatively as a team.
Respect others.
Ask questions before proceeding.
Avoid sudden movements.
Work quietly.

Contact Information

Email: [email protected] & [email protected]
Office: N30 (2nd floor - Medical Building)
EUC account policy: Faculty emails must come through EUC account.

Materials and Equipment

Pipetman (P1000, P200, P10)
Pipet tips
Distilled Water
1.0 M CuSO4 solution
Cuvettes
Balance machine
Spectrophotometer
Weighing boats
Waste containers

Lab Exercise 1 Details

Introduction to techniques: use of Pipetman.
Sample preparation.
Dilutions.

Pipetman Use

For volumes under 1 mL, the Pipetman is the standard method.
Types: P1000 (200-1000 µL), P100 (10-100 µL), P10 (0.5-10 µL)
1 L = 1000 mL and 1 mL = 1000 µL

Pipetman Procedure (diagram reference)

Pipette Accuracy Check

Weigh water to check pipette accuracy.
Density of water at room temperature is 1 g/mL, meaning each microliter should weigh 0.001 g.

Part A - Pipette Use Exercises

Tables for calculating volume using 1000µl, and 200µl pipettes (various measurement exercises for volumes).

Part B - Sample Preparation

Solvent and solute definitions.
Aqueous solutions defined.
Common concentration units (%w/w, %v/v, %w/v, Molarity).
Concentration calculation example of mass concentration (100g solution, 5g NaOH).

Part C - Dilutions

Dilution formulas (C1V1 = C2V2) for calculating dilutions.
Definitions of C1 (initial/stock solution concentration), V1 (volume of stock solution), C2 (final solution concentration), V2 (final solution volume).
Parallel dilution, and various dilution factors (e.g., 10X, 20X, 100X solutions).
Example dilution problem for converting 10X NaOH to 1X.

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Description

Dive into the foundational techniques of Medical Biochemistry I with a focus on the use of Pipetman, sample preparation, and dilutions. This lab exercise will guide students through important safety protocols and practical exercises to ensure effective laboratory practice. Get ready to engage with key concepts and procedures essential for success in the course.