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Questions and Answers
What is the most important safety protocol to follow before starting a lab experiment?
What is the most important safety protocol to follow before starting a lab experiment?
Which of the following items is NOT required to be worn in the lab?
Which of the following items is NOT required to be worn in the lab?
Which formula is used to calculate reagent concentrations during experiments?
Which formula is used to calculate reagent concentrations during experiments?
What is the maximum number of justified absences permitted?
What is the maximum number of justified absences permitted?
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What is the first step that should be taken if a student has symptoms such as cough or fever?
What is the first step that should be taken if a student has symptoms such as cough or fever?
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Which of the following is an appropriate action while working in the lab?
Which of the following is an appropriate action while working in the lab?
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What is the purpose of a Pipetman in a laboratory setting?
What is the purpose of a Pipetman in a laboratory setting?
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Why is it important to keep a laboratory notebook?
Why is it important to keep a laboratory notebook?
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What should students do with their hair while in the lab?
What should students do with their hair while in the lab?
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Which Pipetman is suitable for dispensing volumes from 10 to 100 μl?
Which Pipetman is suitable for dispensing volumes from 10 to 100 μl?
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How can you check the accuracy of a pipette?
How can you check the accuracy of a pipette?
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What is defined as the substance dissolved in the solvent?
What is defined as the substance dissolved in the solvent?
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If the density of water is 1 g/mL, how much would 500 μl of water weigh?
If the density of water is 1 g/mL, how much would 500 μl of water weigh?
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What is the term for a solution where water acts as the solvent?
What is the term for a solution where water acts as the solvent?
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In a dilution process, what is typically used to prepare a less concentrated solution?
In a dilution process, what is typically used to prepare a less concentrated solution?
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What is the mass concentration percentage if 5 g of NaOH is dissolved in 100 g of solution?
What is the mass concentration percentage if 5 g of NaOH is dissolved in 100 g of solution?
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Study Notes
Course Information
- Course title: Medical Biochemistry I
- Lab exercise 1: Introduction to Techniques - Use of Pipetman, Sample Preparation and Dilutions
- Fall Semester 2024
- Instructors: Andreou M & Kyriakou TC
- Institution: European University Cyprus, School of Medicine
Objectives
- Introduction: Lab characteristics/guidelines
- Part A: Use of Pipetman (Theoretical background and exercises)
- Part B: Sample Preparation (Units of concentration and exercises)
- Part C: Dilutions (Dilution formula and exercises)
COVID-19 Safety Protocol
- Be punctual to avoid overcrowding at the lab entrance.
- Always bring protective gear (lab coat with cuffed sleeves, mask, goggles).
- Students without appropriate gear will not be admitted.
- Maintain hand hygiene (wash hands with soap and put on gloves).
- Disinfect working surfaces before and after experiments.
- Report any symptoms (fever, cough, or difficulty breathing) immediately.
Absences
- Mandatory participation is required.
- Physical presence or synchronous participation (only after permission).
- Only two justified absences are excused (severe health conditions or emergencies).
- Notify the instructor of any justified absence before class.
- Lab experiments cannot be repeated.
Preparation in Advance
- Study uploaded materials.
- Print the protocol or experimental methodology.
- Mark materials/equipment needed on the protocol.
- Prepare mentally for potentially complex procedures.
- Wear closed-toe shoes, lab coat, goggles, and gloves.
- Tie hair back (ponytail).
- No food or drinks allowed in the lab.
Laboratory Notebook
- Record all information and explanations.
- Document protocol applications with dates.
- Perform calculations of reagent concentrations (e.g., C1V1=C2V2).
- Analyze results and identify areas for improvement.
Lab Conduct
- Move as if in a bubble (avoid contact with others).
- Be aware of the surroundings in the lab.
- Work as a team with mutual respect.
- Raise your hand to ask questions.
- Avoid sudden movements.
- Work quietly.
Contact Information
- Email: [email protected] & [email protected]
- For questions, clarifications, or issues: contact through email
- Office number: N30, 2nd floor, Medical Building
EUC Account Policy
- If a student sends an email to a faculty member through a personal email account not associated with the institution, inform them that the message must be sent from their EUC account.
Materials and Equipment
- Pipetman (P1000, P200, P10)
- Pipet tips
- Distilled water
- 1.0 M CuSO4 solution
- Cuvettes
- Balance machine
- Spectrophotometer
- Weighing boats
- Waste containers
Lab Exercise 1 Details
- Introduction to Techniques (Use of Pipetman, Sample Preparation, and Dilutions)
Pipetman Use
- For volumes under 1 mL, a Pipetman is the standard method.
- Pipettes are essential in well-equipped laboratories.
- Pipetman types in this course:
- P1000 (200-1000 µL)
- P100 (10-100 µL)
- P10 (0.5-10 µL)
- 1 liter = 1000 milliliters and 1 milliliter = 1000 microliters
Pipetman Procedure (Diagram)
- Instructions and steps involved in using the Pipetman are not included.
- Diagrams are present of the different types of pipetmen and use of it
Part A - Use of Pipetman
- Method to check pipette accuracy: Weigh water.
- Water density at room temperature = 1 g/mL.
- Each microliter (μL) of water should weigh 0.001 g.
- Accurate dispensing equals weight of the dispensed water.
Part A - Use of 1000μl Pipetman
- Need to weigh accurately the volumes of distilled water. (Measurements need to be taken and recorded).
- Table to record readings.
Part A - Use of 200μl Pipetman
- Need to weigh accurately the volumes of distilled water. (Measurements need to be taken and recorded).
- Table to record readings.
Part B - Sample Preparation
- Solvent: Liquid forming the bulk of the solution.
- Aqueous solution: Solution with water as the solvent.
- Solute: Substance dissolved in the solvent.
- Solution concentration: Amount of solute per unit volume (can be expressed using mass or volume).
Part B - Sample Preparation (Units of Concentration)
- % by mass (mass/mass)
- % by volume (volume/volume)
- % Weight by volume (mass/volume)
- Molarity (M)
Example Calculation
- Calculate the % by mass concentration given mass of solute and total mass of solution. (example calculation needed).
Part C - Dilutions
- Many biochemical solutions prepared by diluting a stock (more concentrated) solution.
- To dilute, consider final concentration and desired volume (or final amount) of the diluted solution.
- Dilution formula: C1V1 = C2V2
- C1 = Initial concentration
- V1 = Initial volume
- C2 = Final Concentration
- V2 = Final volume
Parallel Dilutions
- Can apply the dilution formula even if the concentration of the initial solution is unknown, as long as the concentration multiple is known ('10X'/'20X' and so on).
- The 'X' factor indicates the dilution multiple, which should be converted to the desired ratio before use.
- Example of calculation (10X stock solution diluted to a 1X solution to make a total of 50 mL).
Specific Procedures
- Specific procedures, measurements, and detailed exercises are not included. These are referenced as required tasks.
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Description
This quiz covers the introductory lab techniques for Medical Biochemistry I, focusing on the use of Pipetman, sample preparation, and dilution methods. It includes theoretical backgrounds and exercises designed to enhance practical skills in a laboratory setting. Ensure familiarity with safety protocols for lab participation.
