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Questions and Answers
What is a requirement for students regarding personal protective equipment in the lab?
What is a requirement for students regarding personal protective equipment in the lab?
How should students prepare for lab experiments according to the guidelines?
How should students prepare for lab experiments according to the guidelines?
What must be done immediately if a student exhibits symptoms like fever or cough?
What must be done immediately if a student exhibits symptoms like fever or cough?
What is the maximum number of justified absences allowed?
What is the maximum number of justified absences allowed?
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What should a student do with their laboratory notebook during the lab?
What should a student do with their laboratory notebook during the lab?
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How should students communicate and move in the lab?
How should students communicate and move in the lab?
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Which formula is essential for making calculations of reagent concentrations?
Which formula is essential for making calculations of reagent concentrations?
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What is a key characteristic of the lab environment as outlined in the guidelines?
What is a key characteristic of the lab environment as outlined in the guidelines?
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What is the primary purpose of using a Pipetman in a laboratory setting?
What is the primary purpose of using a Pipetman in a laboratory setting?
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Which Pipetman should be used to measure a volume of 25 μl?
Which Pipetman should be used to measure a volume of 25 μl?
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How can the accuracy of a Pipetman be verified?
How can the accuracy of a Pipetman be verified?
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In the context of a liquid solution, what is the role of the solvent?
In the context of a liquid solution, what is the role of the solvent?
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What is a key characteristic of an aqueous solution?
What is a key characteristic of an aqueous solution?
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What is the % by mass concentration if 5 g of NaOH is dissolved in 100 g of solution?
What is the % by mass concentration if 5 g of NaOH is dissolved in 100 g of solution?
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What is an essential piece of equipment for checking the accuracy of a pipetted volume of liquid?
What is an essential piece of equipment for checking the accuracy of a pipetted volume of liquid?
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What is the volume range for the P200 Pipetman?
What is the volume range for the P200 Pipetman?
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Study Notes
Course Information
- Course name: Medical Biochemistry I
- Course code: MD100
- Lab Exercise 1 title: Introduction to Techniques - Use of Pipetman, Sample Preparation and Dilutions
- Semester: Fall 2024
- Instructors: Andreou M & Kyriakou TC
- University: European University Cyprus
- School: School of Medicine
Objectives
- Introduction: Lab characteristics/guidelines
- Part A: Use of Pipetman (theoretical background and exercises)
- Part B: Sample Preparation (main units of concentration and exercises)
- Part C: Dilutions (dilution formula and exercises)
COVID-19 Safety Protocol
- Be on time to avoid crowds at the lab entrance.
- Carry all protective gear (lab coat with cuffed sleeves, mask, goggles).
- Students without protective gear are not allowed in the lab.
- Practice hand hygiene (wash hands with soap, then gloves).
- Disinfect working surfaces before and after experiments.
- Report any symptoms (fever, cough, breathing difficulties) immediately.
Absences
- Mandatory participation: physical presence or synchronous participation required (permission from the department).
- Excused absences: only two justified absences allowed (severe health condition or emergency).
- Announcements: justified absences must be announced before the beginning of the class.
- Repeating experiments: Lab experiments are not repeatable.
Preparation
- Study uploaded materials.
- Print the protocol/experimental methodology.
- Mark materials/equipment needed on the protocol.
- Mental preparation for demanding applications.
- Required attire: Closed-toe shoes, lab coat, goggles, gloves, hair tied back (ponytail).
- No food or drinks in the lab.
Lab Notebook
- Record all information and explanations.
- Note down protocol applications with dates.
- Calculate reagent concentrations (e.g., C1V1=C2V2).
- Evaluate results and identify areas for improvement.
Lab Etiquette
- Move cautiously (like in a bubble).
- Be aware of your surroundings.
- Work collaboratively as a team.
- Raise your hand to ask questions or for clarification.
- Avoid sudden or rushed movements.
- Work quietly and respectfully.
Contact Information
- Email addresses: [email protected] & [email protected]
- Office location: N30 (2nd floor - Medical Building)
- Email policy: Students must use their EUC email when contacting faculty members.
Materials and Equipment
- Pipetman (P1000, P200, P10)
- Pipet tips
- Distilled Water
- 1.0 M CuSO4 solution
- Cuvettes
- Balance machine
- Spectrophotometer
- Weighing boats
- Waste containers
Pipetman Use
- For volumes less than 1 mL, Pipetman is the most common method.
- Pipettes are basic tools in a fully equipped laboratory.
- Types of Pipetman:
- P1000 (200-1000 μL)
- P100 (10-100 μL)
- P10 (0.5-10 μL)
- 1L = 1000 mL and 1 mL = 1000 μL
Pipetman Procedure (Diagram-based)
- Illustration of different steps in using a Pipetman (A-E).
- Diagram displays steps: upper stop, first stop, second stop.
- Illustrations show important measurements (1 cm, 30 degrees).
Part A - Pipetman Accuracy
- Accuracy checking method: weighing water.
- Water density at room temperature: 1 g/mL
- Each microliter (μL) should weigh 0.001 g (water).
- Accurate pipette volume delivery equals water weight.
Part A – 1000 μL Pipetman Exercise
- Instructions to accurately weigh different volumes of distilled water (1000 μL, 650 μL, 358 μL).
- Table to record readings and calculate averages.
Part A – 200 μL Pipetman Exercise
- Instructions for accurately weighing various volumes of distilled water (200 μL, 65 μL, 47.5 μL).
- Table for recording readings and calculating averages.
Part B – Sample Preparation
- Solvent: the liquid that makes up most of a solution.
- Aqueous solution: a solution using water as a solvent.
- Solute: the substance dissolved in the solvent.
- Definitions: Solution concentration = amount of solute / amount of solution.
Concentration Units
- % by mass (mass/mass)
- % by volume (volume/volume)
- % weight/volume (mass/volume)
- Molarity (M)
Part B – Example 1: % by Mass
- Calculation example to determine the % by mass concentration of a 100 g solution, where 5 g of NaOH is dissolved in water.
- Formula for calculation presented (g solute/g solution * 100%).
Part C – Dilutions
- Many biochemical solutions are prepared by diluting a concentrated stock solution.
- Dilutions require consideration of both the final desired concentration and volume of the diluted material.
- Important formula: C1V1 = C2V2
- Definitions:
- C1: Initial/stock solution concentration
- V1: Volume of the stock solution
- C2: Desired final solution concentration
- V2: Desired final solution volume
- Parallel dilutions: Using the same equation to dilute solutions with known concentration factors (eg 10X, 20X, 100X).
Part C – Parallel Dilution Example
- Example calculating the volume needed to prepare 50 mL of 1X NaOH from a 10X NaOH stock solution.
- Dilution factors indicated by numerical suffixes like 0.5X, 1X, 2X.
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Description
This quiz covers the foundational techniques in Medical Biochemistry I, focusing on the use of Pipetman, sample preparation, and dilutions. Understand lab safety protocols and guidelines crucial for conducting experiments effectively. Prepare to apply theoretical knowledge in practical scenarios with a strong emphasis on accuracy and precision.
